FastQCFastQC Report
Wed 25 May 2016
SRR1033181_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033181_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2772871
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21300.033.6099171
TCAACGC29800.033.5742154
ATCAACG30550.032.448313
GTATCAA32550.032.4116021
CAACGCA31550.031.5636235
GTACATG67350.029.650481
AACGCAG33900.029.5104246
TACATGG67500.028.0660172
ACATGGG69900.026.0108573
ACGCAGA39000.025.1701057
TATCAAC40100.025.087012
CGCAGAG40350.023.9787778
TAGACCG1050.00234406922.36825
CATGGGG42300.020.654524
GAGTACA46950.018.7589911
GCAGAGT50900.018.362799
CTGTCGC7250.016.8437469
AGTACAT51100.016.4668922
CATGGGC27200.016.4057924
AGGACCG3151.5234218E-716.4033475
Produced by FastQC (version 0.11.5)