##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033181_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2772871
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.669521229079894	33.0	31.0	34.0	30.0	34.0
2	31.73663397972715	34.0	31.0	34.0	30.0	34.0
3	31.91997283681787	34.0	31.0	34.0	30.0	34.0
4	35.25942065101478	37.0	35.0	37.0	33.0	37.0
5	35.3733513026751	37.0	35.0	37.0	33.0	37.0
6	35.427767465561864	37.0	36.0	37.0	35.0	37.0
7	35.375530632330175	37.0	37.0	37.0	33.0	37.0
8	35.392116690606954	37.0	37.0	37.0	35.0	37.0
9	37.05219427806054	39.0	38.0	39.0	35.0	39.0
10-11	37.042946822985996	39.0	38.0	39.0	34.5	39.0
12-13	36.99601099365964	39.0	38.0	39.0	34.0	39.0
14-15	38.46436671594171	41.0	39.0	41.0	35.0	41.0
16-17	38.47530609970677	41.0	39.0	41.0	34.5	41.0
18-19	38.47097160307854	41.0	39.0	41.0	34.0	41.0
20-21	38.50939838167733	41.0	39.0	41.0	34.0	41.0
22-23	38.47299531784927	41.0	39.0	41.0	34.0	41.0
24-25	38.468181173952914	41.0	39.0	41.0	34.0	41.0
26-27	38.429638089907534	41.0	39.0	41.0	34.0	41.0
28-29	38.39878991846357	40.5	39.0	41.0	34.0	41.0
30-31	38.39380284910477	41.0	39.0	41.0	34.0	41.0
32-33	38.45930138834443	41.0	39.0	41.0	34.0	41.0
34-35	38.432094929767736	41.0	39.0	41.0	34.0	41.0
36-37	38.35048006200072	41.0	39.0	41.0	34.0	41.0
38-39	38.27442567649199	41.0	39.0	41.0	34.0	41.0
40-41	38.21937966100839	41.0	39.0	41.0	33.0	41.0
42-43	38.11027469362982	40.5	38.0	41.0	33.0	41.0
44-45	37.98029316906556	40.0	38.0	41.0	33.0	41.0
46-47	37.864664457884984	40.0	38.0	41.0	33.0	41.0
48-49	37.7546499638822	40.0	38.0	41.0	33.0	41.0
50-51	37.64135944297445	40.0	38.0	41.0	32.5	41.0
52-53	37.50474165585056	40.0	37.5	41.0	32.0	41.0
54-55	37.363831746951085	40.0	37.0	41.0	32.0	41.0
56-57	37.21831614236652	40.0	37.0	41.0	31.5	41.0
58-59	37.034040169917745	40.0	36.5	41.0	31.0	41.0
60-61	36.8229993389523	40.0	36.0	41.0	31.0	41.0
62-63	36.57030330657287	39.0	35.5	41.0	31.0	41.0
64-65	36.30913194302944	39.0	35.0	41.0	30.5	41.0
66-67	36.02941463919527	38.5	35.0	41.0	30.0	41.0
68-69	35.72920305344172	38.0	35.0	40.0	30.0	41.0
70-71	35.38239878450891	37.0	35.0	40.0	30.0	41.0
72-73	35.012281314204664	37.0	35.0	39.5	29.0	41.0
74-75	34.64203022787572	36.0	34.0	39.0	29.0	41.0
76-77	34.304027305994396	36.0	34.0	39.0	29.0	40.5
78-79	33.9277454667022	35.0	34.0	37.5	29.0	39.5
80-81	33.550920327703665	35.0	34.0	37.0	28.5	39.0
82-83	33.30987359310981	35.0	34.0	37.0	29.0	39.0
84-85	33.021848834655486	35.0	34.0	36.0	28.0	38.0
86-87	32.75115917761771	35.0	34.0	36.0	28.0	37.0
88-89	32.37214208666757	35.0	34.0	36.0	27.0	37.0
90-91	32.169454330908295	35.0	34.0	35.0	27.0	36.5
92-93	31.983889441665337	35.0	34.0	35.0	26.5	36.0
94-95	31.806602795441982	35.0	33.5	35.0	26.0	36.0
96-97	31.623159173290066	35.0	33.0	35.0	25.5	36.0
98-99	31.427149153350445	35.0	33.0	35.0	25.0	35.5
100	31.265372604783995	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.15628342718706278
1101	2	0.1165133093530315
1101	3	0.18090292918626005
1101	4	0.15050776202862437
1101	5	0.15581510571442436
1101	6	0.17224076113341624
1101	7	0.18833606042098694
1101	8	0.14940454059081532
1101	9	0.26505990655277856
1101	10-11	0.27512826807232926
1101	12-13	0.2721092472353348
1101	14-15	0.2742358502933726
1101	16-17	0.1986477919545777
1101	18-19	0.23282493935896298
1101	20-21	0.14102115773108892
1101	22-23	0.21978798921090004
1101	24-25	0.18112511952211463
1101	26-27	0.20209689175537449
1101	28-29	0.18244336373750514
1101	30-31	0.20480883944058803
1101	32-33	0.2579753565940308
1101	34-35	0.22253642012036323
1101	36-37	0.22806700477574537
1101	38-39	0.2305547290139387
1101	40-41	0.24857295984447347
1101	42-43	0.22305965827937513
1101	44-45	0.21851392088599653
1101	46-47	0.21407614836203237
1101	48-49	0.1757417705249864
1101	50-51	0.1273611850335854
1101	52-53	0.08807230709136604
1101	54-55	0.07353172381229456
1101	56-57	0.09339880687578983
1101	58-59	0.0861584813985985
1101	60-61	0.03305132159982094
1101	62-63	0.023450623959476502
1101	64-65	0.040852058075671493
1101	66-67	0.08971051858588197
1101	68-69	0.10304991183271994
1101	70-71	0.05914147472292797
1101	72-73	0.11881634969711996
1101	74-75	-0.013415695411076456
1101	76-77	-0.016365809135848508
1101	78-79	-0.0021253559869833794
1101	80-81	-0.03259434150606921
1101	82-83	-0.04582344598399146
1101	84-85	-0.04809143307990382
1101	86-87	0.006086024090230069
1101	88-89	0.22357456017451938
1101	90-91	0.24243629469449957
1101	92-93	0.2440486885106523
1101	94-95	0.2542706608425753
1101	96-97	0.21701898622922755
1101	98-99	0.25090234870510386
1101	100	0.26447412545284266
1102	1	0.21978376131897548
1102	2	0.2292405791186347
1102	3	0.20604447202457976
1102	4	0.2653226944875513
1102	5	0.16789801603798082
1102	6	0.1478470955088511
1102	7	0.09299545966769784
1102	8	0.17845182280134964
1102	9	0.21366650929253694
1102	10-11	0.2099627719872288
1102	12-13	0.17979273370736593
1102	14-15	0.24997585779454568
1102	16-17	0.19259462894095947
1102	18-19	0.2289880217940521
1102	20-21	0.2556464229902886
1102	22-23	0.29950400355678397
1102	24-25	0.2340630369579344
1102	26-27	0.25647375508544457
1102	28-29	0.23925006445671215
1102	30-31	0.2876823087153184
1102	32-33	0.2560241158264347
1102	34-35	0.26423734497956985
1102	36-37	0.30767021651438853
1102	38-39	0.3067161377352505
1102	40-41	0.3429369221399057
1102	42-43	0.27391052386631287
1102	44-45	0.21298954554570315
1102	46-47	0.18244346704405956
1102	48-49	0.19800308000444033
1102	50-51	0.23773242778032255
1102	52-53	0.12467298626894774
1102	54-55	0.16144109648726612
1102	56-57	0.21115841678238212
1102	58-59	0.22691047977620116
1102	60-61	0.09366846995805389
1102	62-63	0.17539614315078467
1102	64-65	0.19786188493384316
1102	66-67	0.18246134251322843
1102	68-69	0.15331640290197157
1102	70-71	0.16804618176804098
1102	72-73	0.215428626774532
1102	74-75	0.17208951570287212
1102	76-77	0.1570389807804986
1102	78-79	0.15151159146858362
1102	80-81	0.14717890896417885
1102	82-83	0.19648032125657977
1102	84-85	0.20295596152601547
1102	86-87	0.1995448370891637
1102	88-89	0.28413683434023795
1102	90-91	0.31443530756988025
1102	92-93	0.3812661373229034
1102	94-95	0.3767268500610079
1102	96-97	0.4118853884409788
1102	98-99	0.3004953837753135
1102	100	0.34131224801022597
1103	1	0.03432769971054128
1103	2	-0.005835821341953817
1103	3	-0.04139834961302924
1103	4	-0.020999522266500037
1103	5	-0.03602269964491711
1103	6	-0.028198245014621648
1103	7	0.04875925215769428
1103	8	-0.006661766891419063
1103	9	0.011051389804315193
1103	10-11	0.036210778393531484
1103	12-13	-0.005889037579706269
1103	14-15	-0.00718636934596617
1103	16-17	-0.007274246672842821
1103	18-19	-0.05280752398272881
1103	20-21	-0.07097232650322383
1103	22-23	-0.04554055588117478
1103	24-25	-0.015278418771643487
1103	26-27	0.01235914973195662
1103	28-29	0.012668280915512753
1103	30-31	-0.011220809230437112
1103	32-33	-0.01761902226441947
1103	34-35	0.009625072441011184
1103	36-37	0.016445561898784433
1103	38-39	-0.011233376847748389
1103	40-41	-0.004483958967121282
1103	42-43	-0.03447525725137979
1103	44-45	-0.09182933967571216
1103	46-47	-0.019963837990260913
1103	48-49	-0.05666668710021128
1103	50-51	-0.049319782802015766
1103	52-53	-0.16851144172021293
1103	54-55	-0.15159376551198278
1103	56-57	-0.18659299733439383
1103	58-59	-0.16035321624376309
1103	60-61	-0.1687591890063871
1103	62-63	-0.13758148696457084
1103	64-65	-0.1549394827998185
1103	66-67	-0.19682594289347577
1103	68-69	-0.285104027311057
1103	70-71	-0.19043296316989
1103	72-73	-0.12375697041309053
1103	74-75	-0.15139504114502955
1103	76-77	-0.1537863299101616
1103	78-79	-0.1641918918155696
1103	80-81	-0.10295387688692159
1103	82-83	-0.09841830508626259
1103	84-85	-0.07972708889202096
1103	86-87	-0.037087922559386755
1103	88-89	-0.0031597691920737248
1103	90-91	-0.0067510755628745756
1103	92-93	0.03472560276726
1103	94-95	-0.031185965192104703
1103	96-97	-0.024117052139324358
1103	98-99	0.0062974293603943465
1103	100	0.10307604338972709
1104	1	0.08725760115154912
1104	2	0.03232411885328901
1104	3	0.07630079366503395
1104	4	0.12558112814152622
1104	5	0.017380572726345633
1104	6	0.042658070299083306
1104	7	0.10162284288294643
1104	8	0.05806474832407815
1104	9	0.10663196827147203
1104	10-11	0.10934568003784051
1104	12-13	0.13357938194192087
1104	14-15	0.05713577561802197
1104	16-17	0.10457012958416811
1104	18-19	0.026123747462698077
1104	20-21	0.04172902191892547
1104	22-23	0.017668663810610497
1104	24-25	0.015675079275006
1104	26-27	0.04856445646593244
1104	28-29	0.10670432016395637
1104	30-31	0.10918471833073795
1104	32-33	0.057250811677285185
1104	34-35	0.09632420633548122
1104	36-37	0.10842612535220297
1104	38-39	0.045387873234354004
1104	40-41	0.0654452261930416
1104	42-43	0.01496019458421216
1104	44-45	8.101395975046444E-4
1104	46-47	0.028910068430299418
1104	48-49	-0.05570023969680449
1104	50-51	-0.11170731263401734
1104	52-53	-0.07622365946959064
1104	54-55	-0.14960435020359597
1104	56-57	-0.20996536640983265
1104	58-59	-0.11876776456456639
1104	60-61	-0.06255563387621521
1104	62-63	-0.10653327719750649
1104	64-65	-0.20110841247606004
1104	66-67	-0.03130341758123478
1104	68-69	-0.09383583487235825
1104	70-71	-0.06584645114481447
1104	72-73	-0.024066409993700688
1104	74-75	-0.08736078477904385
1104	76-77	-0.10367507178943924
1104	78-79	-0.06194846267576537
1104	80-81	0.010986293331441743
1104	82-83	-0.008867872971862312
1104	84-85	0.03517818374824344
1104	86-87	0.10485213505587154
1104	88-89	0.27450509269709045
1104	90-91	0.16316675545114379
1104	92-93	0.15530488317087787
1104	94-95	0.18646572838983033
1104	96-97	0.14761124908648426
1104	98-99	0.18328420162374925
1104	100	0.22605045433474658
1105	1	0.1724958171888531
1105	2	0.12331612194574859
1105	3	0.21942683555820253
1105	4	0.1498815799305646
1105	5	0.12267037002527559
1105	6	0.15406501866950606
1105	7	0.06494385469711261
1105	8	0.09282447870024413
1105	9	0.14946096491325278
1105	10-11	0.12848341529430485
1105	12-13	0.15728435403730145
1105	14-15	0.17135456714274966
1105	16-17	0.21939968190230275
1105	18-19	0.1691710886721367
1105	20-21	0.22401158334532312
1105	22-23	0.17421018249824272
1105	24-25	0.223648430969817
1105	26-27	0.2369889932009741
1105	28-29	0.242378681740135
1105	30-31	0.17119107236905506
1105	32-33	0.17475177807094866
1105	34-35	0.19377057148732746
1105	36-37	0.2076813378380038
1105	38-39	0.244482060376491
1105	40-41	0.21992506480530238
1105	42-43	0.18190186064688163
1105	44-45	0.17196612761422614
1105	46-47	0.24248663096805245
1105	48-49	0.23090216179144107
1105	50-51	0.28914800615508796
1105	52-53	0.22663142980103146
1105	54-55	0.12793750672182114
1105	56-57	0.13462113790269825
1105	58-59	0.1528684607017965
1105	60-61	0.16661985113260158
1105	62-63	0.21728162217674907
1105	64-65	0.22215576331095122
1105	66-67	0.20868281130910304
1105	68-69	0.14460784853708475
1105	70-71	0.18962357857179057
1105	72-73	0.2192818420417595
1105	74-75	0.17967401747265654
1105	76-77	0.1931990071166041
1105	78-79	0.2094427817976694
1105	80-81	0.22501416170862143
1105	82-83	0.15323530163131238
1105	84-85	0.21371917091936155
1105	86-87	0.23973073185227634
1105	88-89	0.24083050160241015
1105	90-91	0.20150486035316817
1105	92-93	0.1671729221165421
1105	94-95	0.17878567717096772
1105	96-97	0.2762338361223122
1105	98-99	0.27149891013526073
1105	100	0.32132442693748686
1106	1	0.11993708911662893
1106	2	0.07604608867626084
1106	3	0.0523607392168266
1106	4	0.05311838256289292
1106	5	0.08753239160581217
1106	6	0.06571545684343505
1106	7	-0.04331496185631778
1106	8	-0.0039232240491458015
1106	9	0.06883061377745037
1106	10-11	0.09570293148293985
1106	12-13	0.06349499597239827
1106	14-15	0.08989947447799551
1106	16-17	-0.0033046978943787053
1106	18-19	0.018810763636672334
1106	20-21	0.02779036806771984
1106	22-23	0.051783734526168246
1106	24-25	0.016395365463665712
1106	26-27	0.0686423938534162
1106	28-29	0.12924943835390934
1106	30-31	0.04351489740290049
1106	32-33	0.026788031083725627
1106	34-35	0.014029328143649877
1106	36-37	0.0709876130196534
1106	38-39	0.022364597062889402
1106	40-41	0.011889752338980486
1106	42-43	0.03557441472887746
1106	44-45	0.032481810480547324
1106	46-47	0.07175348460311426
1106	48-49	0.05683469498577409
1106	50-51	-0.015269526861168004
1106	52-53	-0.06405495172457165
1106	54-55	-0.0018696407837097695
1106	56-57	-0.03978223835149208
1106	58-59	-0.11471093534110821
1106	60-61	-0.09567671840516567
1106	62-63	-0.050022368589374366
1106	64-65	-0.03799520630526132
1106	66-67	-0.10376339114496602
1106	68-69	-0.11826294692886563
1106	70-71	-0.07298406855422712
1106	72-73	-0.018492354886042506
1106	74-75	-0.010695898037745621
1106	76-77	0.008731124685141367
1106	78-79	-0.12997405858602207
1106	80-81	-0.06737509571064493
1106	82-83	-0.13219831757387368
1106	84-85	-0.07079728829671694
1106	86-87	-0.0883027096967055
1106	88-89	-0.04249582060887036
1106	90-91	-0.06741866491762494
1106	92-93	-0.06506423249800974
1106	94-95	-0.05550260644622895
1106	96-97	-0.05770319953057168
1106	98-99	0.004012366170552895
1106	100	0.021195810042616614
1107	1	0.20131552200074765
1107	2	0.16603243776410181
1107	3	0.21474662267161193
1107	4	0.157173951494471
1107	5	0.18437318839497152
1107	6	0.1206048800371704
1107	7	0.1386183430560166
1107	8	0.12573868394193966
1107	9	0.16834824841454576
1107	10-11	0.1137976241938432
1107	12-13	0.10019499287995615
1107	14-15	0.06226413606569281
1107	16-17	0.08198244064912075
1107	18-19	0.1241674765500349
1107	20-21	0.10980794196046162
1107	22-23	0.14176235251488833
1107	24-25	0.08393543464595155
1107	26-27	0.11028535181610977
1107	28-29	0.1487078969494604
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2216	8	-0.0021314350181782515
2216	9	-0.033007866855250256
2216	10-11	-0.03828174005212048
2216	12-13	-0.13082389372539893
2216	14-15	-0.12443854616229544
2216	16-17	-0.14888155779074452
2216	18-19	-0.09902824508630204
2216	20-21	-0.05970177886580785
2216	22-23	-0.10025303129273055
2216	24-25	-0.11474126537355289
2216	26-27	-0.22509515552700776
2216	28-29	-0.14404142424284316
2216	30-31	-0.17091599902653343
2216	32-33	-0.16078770168893897
2216	34-35	-0.16563019589982275
2216	36-37	-0.22061386434321406
2216	38-39	-0.12246064944562107
2216	40-41	-0.07944112618394428
2216	42-43	-0.10392374017987294
2216	44-45	-0.02822791798924129
2216	46-47	0.007986916814267886
2216	48-49	0.039562477541572605
2216	50-51	0.0445694644105501
2216	52-53	-0.03576852402381547
2216	54-55	-0.039145102834233114
2216	56-57	-0.07672091007547976
2216	58-59	-0.16487709219074276
2216	60-61	-0.05569154533609577
2216	62-63	-0.05475464731026136
2216	64-65	-0.008677423434576781
2216	66-67	-0.00456258766624984
2216	68-69	-0.013205372949755656
2216	70-71	-0.05443824116918705
2216	72-73	0.02043044909117242
2216	74-75	0.012190123807442887
2216	76-77	-0.05801721355668121
2216	78-79	0.02750029185725822
2216	80-81	0.013079807391243037
2216	82-83	0.058130967762934915
2216	84-85	-0.002802465440282731
2216	86-87	-0.03538937852834323
2216	88-89	-0.05991269855590531
2216	90-91	-0.014310406033509082
2216	92-93	0.02973174047226479
2216	94-95	0.002773121925688571
2216	96-97	-0.0021377749509312594
2216	98-99	-0.031900735855195705
2216	100	0.013297645345982545
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28808.0
3	4691.0
4	2970.0
5	2509.0
6	2193.0
7	2427.0
8	2815.0
9	3037.0
10	3407.0
11	3911.0
12	4442.0
13	4809.0
14	5325.0
15	5517.0
16	5561.0
17	5512.0
18	5605.0
19	5659.0
20	5773.0
21	5938.0
22	6876.0
23	8849.0
24	13356.0
25	18885.0
26	20547.0
27	18063.0
28	18788.0
29	23621.0
30	28752.0
31	34566.0
32	45592.0
33	61644.0
34	86202.0
35	129850.0
36	241357.0
37	530076.0
38	931895.0
39	441867.0
40	1176.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.950255701081204	20.006013516300534	13.13063224417047	23.913098538447787
2	15.199506932943097	20.130646883325877	41.06761871647308	23.602227467257944
3	17.925018882590884	25.398988333391674	30.26349739865922	26.412495385358227
4	11.728097531268626	16.58236488504893	40.04591981782156	31.64361776586088
5	12.261256457233074	38.37340864697381	36.528691076733466	12.83664381905965
6	30.568120914054393	38.89134946299773	17.886766783980747	12.653762838967126
7	26.925332438895076	32.651360231399835	23.013932318434442	17.409375011270644
8	24.63272609752948	37.185071420481634	20.65516686660987	17.52703561537901
9	24.627295553156486	16.850630741700876	21.510793468839488	37.01128023630315
10-11	23.339586267496088	27.09980545024282	29.704016360431606	19.856591921829487
12-13	23.955405129766287	24.511253950285067	29.533749587229813	21.99959133271883
14-15	21.388443220298633	26.63623896429185	26.45112618689321	25.524191628516313
16-17	20.991353337963595	30.03170785417309	26.954327235659818	22.022611572203495
18-19	20.812696516422342	29.114170608267138	28.958797064296693	21.11433581101383
20-21	22.133314383428594	28.48525992601962	28.305999358276384	21.075426332275395
22-23	21.58454285755508	28.260755868476867	28.40629553750487	21.748405736463187
24-25	21.592764571724366	28.408633238853366	28.319534087189112	21.67906810223316
26-27	21.318886404838633	28.2549595037286	28.461697171429044	21.964456920003723
28-29	21.2528157469011	28.34068869246543	28.540031850177915	21.86646371045555
30-31	21.658541441713272	28.91629019380244	28.06680725821767	21.358361106266617
32-33	21.120576150985322	28.55439201497733	28.681601910402964	21.643429923634383
34-35	21.305241949227966	28.253010016586405	28.764434652869493	21.67731338131614
36-37	21.71337466133067	28.710376770530072	28.111087146579262	21.46516142156
38-39	21.51362456517297	28.804706059724573	28.240632452068258	21.441036923034197
40-41	21.19588455058211	28.4857439275517	28.597839845301863	21.720531676564324
42-43	21.2552747181407	28.267818629042146	28.64431628832962	21.83259036448753
44-45	21.382165091660983	28.498408824383876	28.55433034742576	21.565095736529376
46-47	21.414976919217803	28.73944197128166	28.397783638991246	21.447797470509293
48-49	21.303328513216734	28.563221157877482	28.446910824596984	21.6865395043088
50-51	21.376327317291675	28.40758351612761	28.43751825838948	21.778570908191227
52-53	21.38527373483634	28.397209904550312	28.4284073265252	21.789109034088142
54-55	21.53914982037049	28.869310537525283	28.200597917513058	21.390941724591165
56-57	21.34443137903358	28.558671999662433	28.26319455727161	21.833702064032373
58-59	21.166387977204987	28.151422416225913	28.713832356071904	21.9683572504972
60-61	21.533482918493878	28.594664667709786	28.292891663401292	21.578960750395044
62-63	21.295805917449133	28.602987702500666	28.337551993932486	21.76365438611771
64-65	21.325394559912176	28.243949402130585	28.49037860404511	21.94027743391213
66-67	21.153132495750718	28.372855658551345	28.32752276472228	22.146489080975655
68-69	21.70393665574504	28.469823661048366	28.2606837529776	21.565555930228992
70-71	21.560118327937168	28.304341906789247	28.34163269832597	21.79390706694761
72-73	21.288937471757	28.397031027358153	28.53083026574837	21.783201235136474
74-75	21.370586083089464	28.43510717390485	28.37727767877059	21.8170290642351
76-77	21.322450769605013	28.442178505950857	28.236913234443666	21.998457490000465
78-79	21.50872708905827	28.57345593736526	28.29501641767118	21.622800555905286
80-81	21.449892336691416	28.251046924468344	28.437088688923996	21.861972049916247
82-83	21.219645589999008	28.360391668294078	28.610805914803617	21.80915682690329
84-85	21.22990556055254	28.255773443422527	28.486083651725956	22.028237344298976
86-87	21.439075272256925	28.434779425593927	28.31733473216281	21.808810569986345
88-89	21.20851664105453	28.27826406869942	28.429070592437615	22.084148697808434
90-91	21.198081768674307	28.1362305651351	28.55613395687463	22.109553709315964
92-93	21.346199097132377	28.412245806553095	28.314277612471567	21.927277483842957
94-95	21.11774370237908	28.082708274038186	28.54292025453332	22.256627769049413
96-97	21.11817151149608	28.10141943676024	28.607373815246472	22.17303523649721
98-99	21.163502806760604	28.161929598713137	28.475863781551002	22.198703812975257
100	21.347446356026346	28.050503298851332	28.382093546179927	22.219956798942395
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	234.0
1	246.0
2	253.0
3	221.5
4	172.0
5	140.5
6	134.5
7	145.5
8	162.0
9	166.0
10	164.0
11	161.0
12	169.0
13	218.0
14	275.5
15	339.0
16	460.0
17	626.0
18	876.0
19	1297.5
20	1935.0
21	2861.0
22	4471.5
23	7085.0
24	10135.0
25	13859.0
26	18855.0
27	25278.0
28	33402.0
29	42083.0
30	50633.0
31	59408.0
32	68134.0
33	76568.5
34	84477.0
35	91223.5
36	96416.5
37	100640.0
38	103412.0
39	104380.0
40	104851.0
41	104108.0
42	101849.5
43	99443.5
44	96817.0
45	94558.5
46	92860.5
47	89929.5
48	86466.0
49	83879.5
50	81239.5
51	77682.5
52	72861.5
53	68853.5
54	65407.0
55	61602.5
56	57207.0
57	51705.5
58	46774.0
59	42243.0
60	37119.0
61	31309.5
62	26195.5
63	21778.5
64	17697.5
65	14168.0
66	11024.0
67	8181.5
68	6053.5
69	4637.0
70	3322.0
71	2431.5
72	1892.5
73	1456.5
74	1088.0
75	788.0
76	533.5
77	380.5
78	275.5
79	177.5
80	118.5
81	74.0
82	52.0
83	36.5
84	23.5
85	16.5
86	15.5
87	19.0
88	15.5
89	7.0
90	3.5
91	6.5
92	8.0
93	4.5
94	2.5
95	3.5
96	3.0
97	2.0
98	2.0
99	1.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3280715186534101
2	0.11785618588098762
3	0.06491466786590505
4	0.03905699183265287
5	0.04230272522594812
6	0.009268372023076442
7	0.006527530490960452
8	0.015038564722268003
9	0.018031852184973624
10-11	0.0206464707517948
12-13	0.016030316592441553
14-15	0.018284298115563254
16-17	0.01909573146388707
18-19	0.01875312627237257
20-21	0.023423375988280738
22-23	0.015543456583447265
24-25	0.02446922341500921
26-27	0.01864493515926273
28-29	0.018608871454892782
30-31	0.019384241098846646
32-33	0.024559382675934077
34-35	0.015128723983192871
36-37	0.009196244614336548
38-39	0.013000965425365983
40-41	0.00885363942282205
42-43	0.0076094416220588695
44-45	0.008493002379122577
46-47	0.007861887552648501
48-49	0.004201421559098854
50-51	0.006239020856000875
52-53	0.007429123100209134
54-55	0.0029031282017807536
56-57	0.002542491158081281
58-59	0.0025785548624512283
60-61	0.0032998289498501737
62-63	0.0025785548624512283
64-65	0.002398236340601492
66-67	0.0018031852184973622
68-69	0.0031014785758154634
70-71	0.0024703637493413864
72-73	0.002163822262196835
74-75	0.0023261089318615978
76-77	0.0042374852634688015
78-79	0.0034801474716999094
80-81	0.003840784515399382
82-83	0.002362172636231545
84-85	0.003209669688925305
86-87	0.0027588733843009645
88-89	0.6019212577866045
90-91	0.6130469105847333
92-93	0.6085569793906749
94-95	0.596223192496153
96-97	0.5949068672866499
98-99	0.5914988472236898
100	0.5935003828162219
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2772871.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.94469902397032
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.71845552492817	56.55601833871793
2	13.232522134536481	18.775546003254316
3	3.6879555405938693	7.849226875236474
4	1.3970318278668945	3.964480102196957
5	0.6729554347139413	2.3871310386162836
6	0.36095904732936757	1.5364878583656623
7	0.22603904001611322	1.1225390163127236
8	0.1400054656448622	0.7946116497508451
9	0.1015495050238021	0.6483959162952122
>10	0.4427294886787045	5.1940998126153906
>50	0.015695188042058106	0.7402958043746265
>100	0.004101802625806422	0.43116758426359836
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	3.6063704369947246E-5	0.0
9	0.0	0.0	0.0	7.212740873989449E-5	0.0
10-11	0.0	0.0	0.0	7.212740873989449E-5	0.0
12-13	0.0	1.8031852184973623E-5	0.0	9.015926092486811E-5	0.0
14-15	0.0	3.6063704369947246E-5	0.0	1.4425481747978899E-4	0.0
16-17	0.0	3.6063704369947246E-5	0.0	1.6228666966476262E-4	0.0
18-19	0.0	3.6063704369947246E-5	0.0	1.9835037403470986E-4	0.0
20-21	0.0	3.6063704369947246E-5	0.0	2.3441407840465712E-4	0.0
22-23	0.0	3.6063704369947246E-5	0.0	2.5244593058963076E-4	0.0
24-25	0.0	3.6063704369947246E-5	0.0	2.7047778277460434E-4	0.0
26-27	0.0	3.6063704369947246E-5	0.0	4.147326002543934E-4	0.0
28-29	0.0	3.6063704369947246E-5	0.0	0.0012622296529481538	0.0
30-31	0.0	3.6063704369947246E-5	0.0	0.00497679120305272	0.0
32-33	0.0	3.6063704369947246E-5	0.0	0.0127845831991463	0.0
34-35	0.0	3.6063704369947246E-5	0.0	0.023585662657945504	0.0
36-37	0.0	3.6063704369947246E-5	0.0	0.04340266820923151	0.0
38-39	0.0	3.6063704369947246E-5	0.0	0.07634686215117834	0.0
40-41	0.0	3.6063704369947246E-5	0.0	0.11832501403779694	0.0
42-43	0.0	3.6063704369947246E-5	0.0	0.15994252888071603	0.0
44-45	0.0	3.6063704369947246E-5	0.0	0.2068614082660174	0.0
46-47	0.0	3.6063704369947246E-5	0.0	0.26530264119751695	0.0
48-49	0.0	3.6063704369947246E-5	0.0	0.33111890167267066	0.0
50-51	0.0	3.6063704369947246E-5	0.0	0.40593305638812627	0.0
52-53	0.0	3.6063704369947246E-5	0.0	0.4808193385123217	0.0
54-55	0.0	3.6063704369947246E-5	0.0	0.5595464051519166	0.0
56-57	0.0	3.6063704369947246E-5	0.0	0.6504269401641836	0.0
58-59	0.0	3.6063704369947246E-5	0.0	0.7573017280645222	0.0
60-61	0.0	3.6063704369947246E-5	0.0	0.8701089953337173	0.0
62-63	0.0	3.6063704369947246E-5	0.0	0.986540664892092	0.0
64-65	0.0	3.6063704369947246E-5	0.0	1.1127095346303524	0.0
66-67	0.0	3.6063704369947246E-5	0.0	1.2541694150214706	0.0
68-69	0.0	3.6063704369947246E-5	0.0	1.4120202490487297	0.0
70-71	0.0	3.6063704369947246E-5	0.0	1.5838097048149733	0.0
72-73	0.0	3.6063704369947246E-5	0.0	1.7709442667906297	0.0
74-75	0.0	3.6063704369947246E-5	0.0	1.9547609679642508	0.0
76-77	0.0	3.6063704369947246E-5	0.0	2.1477558819000233	0.0
78-79	0.0	3.6063704369947246E-5	0.0	2.3782029528239863	0.0
80-81	0.0	3.6063704369947246E-5	0.0	2.626573684819813	0.0
82-83	0.0	3.6063704369947246E-5	0.0	2.889964949685723	0.0
84-85	0.0	3.6063704369947246E-5	0.0	3.1479827226005104	0.0
86-87	0.0	3.6063704369947246E-5	0.0	3.421580015803115	0.0
88	0.0	3.6063704369947246E-5	0.0	3.6428308421127418	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2130	0.0	33.609917	1
TCAACGC	2980	0.0	33.574215	4
ATCAACG	3055	0.0	32.44831	3
GTATCAA	3255	0.0	32.411602	1
CAACGCA	3155	0.0	31.563623	5
GTACATG	6735	0.0	29.65048	1
AACGCAG	3390	0.0	29.510424	6
TACATGG	6750	0.0	28.066017	2
ACATGGG	6990	0.0	26.010857	3
ACGCAGA	3900	0.0	25.170105	7
TATCAAC	4010	0.0	25.08701	2
CGCAGAG	4035	0.0	23.978777	8
TAGACCG	105	0.002344069	22.3682	5
CATGGGG	4230	0.0	20.65452	4
GAGTACA	4695	0.0	18.758991	1
GCAGAGT	5090	0.0	18.36279	9
CTGTCGC	725	0.0	16.843746	9
AGTACAT	5110	0.0	16.466892	2
CATGGGC	2720	0.0	16.405792	4
AGGACCG	315	1.5234218E-7	16.403347	5
>>END_MODULE