FastQCFastQC Report
Wed 25 May 2016
SRR1033181_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033181_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2772871
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42430.15301829764168617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36890.1330390054207354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36700.051.6642231
GTATCAA66250.041.616221
TCAACGC78250.034.962744
ATCAACG79150.034.6245773
CAACGCA79450.034.4352955
AACGCAG82000.033.411526
TATCAAC85600.032.01562
ACGCAGA94300.028.9039927
CGCAGAG96100.028.5087958
GTACATG69000.025.501951
GCAGAGT108150.025.0282029
TACATGG67800.024.2663942
ACATGGG71150.022.1988893
GAGTACT73300.021.76534812-13
AGAGTAC99400.021.06177910-11
CAGAGTA108450.020.32249310-11
GTCGTAT1200.00503543519.6037731
GTGGTAT19500.018.8196221
AGTACTT81900.018.76262712-13
GTACTTT86800.018.40725314-15
Produced by FastQC (version 0.11.5)