##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033181_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2772871 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.78497953925733 34.0 31.0 34.0 31.0 34.0 2 32.86835233229386 34.0 31.0 34.0 31.0 34.0 3 32.96179194776821 34.0 31.0 34.0 31.0 34.0 4 36.380768885389905 37.0 37.0 37.0 35.0 37.0 5 36.32050499283955 37.0 37.0 37.0 35.0 37.0 6 36.40864901396423 37.0 37.0 37.0 35.0 37.0 7 36.37942370921691 37.0 37.0 37.0 35.0 37.0 8 36.37328314227384 37.0 37.0 37.0 35.0 37.0 9 38.19362061920659 39.0 39.0 39.0 37.0 39.0 10-11 38.17854581046143 39.0 39.0 39.0 37.0 39.0 12-13 38.14643378649782 39.0 39.0 39.0 37.0 39.0 14-15 39.693542721605155 41.0 40.0 41.0 37.0 41.0 16-17 39.70983341814315 41.0 40.0 41.0 37.0 41.0 18-19 39.72823546425347 41.0 40.0 41.0 37.0 41.0 20-21 39.726411903042006 41.0 40.0 41.0 37.0 41.0 22-23 39.65700045187822 41.0 40.0 41.0 37.0 41.0 24-25 39.61462577956205 41.0 40.0 41.0 37.0 41.0 26-27 39.522601844802736 41.0 40.0 41.0 37.0 41.0 28-29 39.4366104661919 41.0 39.0 41.0 36.5 41.0 30-31 39.34861178179584 41.0 39.0 41.0 36.0 41.0 32-33 39.38233783684852 41.0 39.0 41.0 36.0 41.0 34-35 39.32929876651312 41.0 39.0 41.0 36.0 41.0 36-37 39.247510432328085 41.0 39.0 41.0 35.0 41.0 38-39 39.15772731583979 41.0 39.0 41.0 35.0 41.0 40-41 39.04973094673355 41.0 39.0 41.0 35.0 41.0 42-43 38.943135111586514 41.0 39.0 41.0 35.0 41.0 44-45 38.814109635825105 41.0 39.0 41.0 35.0 41.0 46-47 38.62067276119228 41.0 39.0 41.0 35.0 41.0 48-49 38.45459164887224 41.0 39.0 41.0 35.0 41.0 50-51 38.34080994031096 41.0 38.5 41.0 35.0 41.0 52-53 38.21726614761379 41.0 38.0 41.0 34.0 41.0 54-55 38.092619526836984 40.0 38.0 41.0 34.0 41.0 56-57 37.95090864306346 40.0 38.0 41.0 34.0 41.0 58-59 37.77320654296575 40.0 37.0 41.0 34.0 41.0 60-61 37.59087187972322 40.0 37.0 41.0 33.5 41.0 62-63 37.369504387329954 40.0 36.0 41.0 33.0 41.0 64-65 37.12626263536962 39.0 36.0 41.0 33.0 41.0 66-67 36.857314133978825 39.0 35.0 41.0 33.0 41.0 68-69 36.54057599506072 39.0 35.0 41.0 32.5 41.0 70-71 36.206158166030804 37.5 35.0 40.0 32.0 41.0 72-73 35.85259141157306 37.0 35.0 40.0 32.0 41.0 74-75 35.512464698141386 36.5 35.0 39.0 31.5 41.0 76-77 35.163830015893275 36.0 35.0 39.0 31.0 41.0 78-79 34.81133435345532 36.0 35.0 38.5 31.0 39.5 80-81 34.45843026956537 35.0 35.0 37.0 31.0 39.0 82-83 34.12316422220868 35.0 35.0 37.0 31.0 39.0 84-85 33.83861744740379 35.0 34.0 36.5 31.0 38.0 86-87 33.59213987956886 35.0 34.0 36.0 30.5 37.0 88-89 33.36307909744088 35.0 34.0 36.0 30.0 37.0 90-91 33.15457228266298 35.0 34.0 35.5 30.0 36.5 92-93 32.985762951107354 35.0 34.0 35.0 30.0 36.0 94-95 32.83414554806191 35.0 34.0 35.0 30.0 36.0 96-97 32.709257300465836 35.0 34.0 35.0 30.0 36.0 98-99 32.60412997936074 35.0 34.0 35.0 30.0 36.0 100 32.50706037172303 35.0 34.0 35.0 29.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14253297830102696 1101 2 0.149760261279404 1101 3 0.15131647078440125 1101 4 0.1136168784875835 1101 5 0.09651797337698298 1101 6 0.09623474663629139 1101 7 0.15787431196597623 1101 8 0.12751070439308876 1101 9 0.18386186910094438 1101 10-11 0.1633682056684691 1101 12-13 0.19401013922242782 1101 14-15 0.1999595804908907 1101 16-17 0.19971561860640463 1101 18-19 0.2261633260374012 1101 20-21 0.24993791576603996 1101 22-23 0.2646337145562967 1101 24-25 0.21875326530462758 1101 26-27 0.2695164740981255 1101 28-29 0.21809026617857796 1101 30-31 0.2557160099344955 1101 32-33 0.21717078903979115 1101 34-35 0.21320640191362372 1101 36-37 0.29679932753499827 1101 38-39 0.22080707437391567 1101 40-41 0.24061238188311762 1101 42-43 0.17989900976949258 1101 44-45 0.140186211052594 1101 46-47 0.28196007235939646 1101 48-49 0.21296579725053277 1101 50-51 0.25048141406400504 1101 52-53 0.2664917372974287 1101 54-55 0.2913885007000232 1101 56-57 0.31142922149901864 1101 58-59 0.2915881610046185 1101 60-61 0.2805411704306806 1101 62-63 0.2859321894630966 1101 64-65 0.32396327522221213 1101 66-67 0.28765094922508183 1101 68-69 0.31909055733683545 1101 70-71 0.3141340984945771 1101 72-73 0.3218764038756632 1101 74-75 0.29431880512972697 1101 76-77 0.2684377415615202 1101 78-79 0.2557517686831261 1101 80-81 0.21487797015758048 1101 82-83 0.20907692678102308 1101 84-85 0.22694805144845986 1101 86-87 0.16709280973923768 1101 88-89 0.2131753984169933 1101 90-91 0.2529165574443084 1101 92-93 0.23206213997432457 1101 94-95 0.21354763774408525 1101 96-97 0.23983339282291638 1101 98-99 0.2502566657589753 1101 100 0.2533618444000538 1102 1 0.09910132751835476 1102 2 0.12070300194295669 1102 3 0.10417780868847615 1102 4 0.07070709247453522 1102 5 0.03493039681272592 1102 6 0.048857651592562945 1102 7 0.1114305872320358 1102 8 0.09718533199177415 1102 9 0.137702390648073 1102 10-11 0.10264079988817087 1102 12-13 0.1421485296961862 1102 14-15 0.1936297049443354 1102 16-17 0.1547050367444882 1102 18-19 0.20828523267023513 1102 20-21 0.21762108867125818 1102 22-23 0.19476217107049365 1102 24-25 0.21236037097297356 1102 26-27 0.26276684661143435 1102 28-29 0.22038205272345834 1102 30-31 0.20731177995889283 1102 32-33 0.1718069354746703 1102 34-35 0.2364520872138911 1102 36-37 0.18932796697674092 1102 38-39 0.20261877383682503 1102 40-41 0.2221060350057371 1102 42-43 0.21531587241542383 1102 44-45 0.20109880450670659 1102 46-47 0.18798287640416333 1102 48-49 0.17237274059715446 1102 50-51 0.16753199646345962 1102 52-53 0.17730916118722462 1102 54-55 0.14443113723496026 1102 56-57 0.17280693227395005 1102 58-59 0.20885722210005753 1102 60-61 0.12667943221987343 1102 62-63 0.08096842679162819 1102 64-65 0.06019677580475502 1102 66-67 0.1004267200395077 1102 68-69 0.13796642437962703 1102 70-71 0.1900238443303408 1102 72-73 0.14301911464855976 1102 74-75 0.1299070707265173 1102 76-77 0.07873959237946337 1102 78-79 0.11107428029630029 1102 80-81 0.11446756413742776 1102 82-83 0.03612589247885012 1102 84-85 0.09943610638536882 1102 86-87 0.14430077824572862 1102 88-89 0.13884336699678101 1102 90-91 0.13807108276319013 1102 92-93 0.08875223048925562 1102 94-95 0.0938899305364913 1102 96-97 0.0643026606281154 1102 98-99 0.06290263212913771 1102 100 0.12487641690871243 1103 1 0.09490451971122837 1103 2 0.10855940094040051 1103 3 0.11687159755476983 1103 4 0.07861540272795509 1103 5 0.037106259129423336 1103 6 0.07877295103122606 1103 7 0.081737122909594 1103 8 0.08352145776821374 1103 9 0.10924721020707295 1103 10-11 0.1163077934628376 1103 12-13 0.07605292756095139 1103 14-15 0.16429897190208465 1103 16-17 0.15039997103650649 1103 18-19 0.15911706838668493 1103 20-21 0.13910836769478152 1103 22-23 0.11882518038664358 1103 24-25 0.14949933891826106 1103 26-27 0.19540547746979087 1103 28-29 0.18772471232555432 1103 30-31 0.2207353984770961 1103 32-33 0.18352134660094777 1103 34-35 0.22559346185347806 1103 36-37 0.17819943565786645 1103 38-39 0.20030257355583814 1103 40-41 0.19774712076057455 1103 42-43 0.09433059220641127 1103 44-45 0.14937917519856825 1103 46-47 0.18128513706029992 1103 48-49 0.13607958306125312 1103 50-51 0.09420294747678071 1103 52-53 0.08972004789331578 1103 54-55 0.06402937006673426 1103 56-57 0.08908239947722763 1103 58-59 0.09816944524123272 1103 60-61 0.1306667509740862 1103 62-63 -0.02548268023652156 1103 64-65 0.07974190743021126 1103 66-67 0.07769065641367234 1103 68-69 0.06066680262885171 1103 70-71 0.05018080613753284 1103 72-73 0.032994084975321414 1103 74-75 -0.0020761220784066836 1103 76-77 0.013823728830779203 1103 78-79 -0.00403798416478196 1103 80-81 0.0690121172053324 1103 82-83 0.057830140430880306 1103 84-85 0.047405921048635946 1103 86-87 0.058089203233471665 1103 88-89 0.05045888484255556 1103 90-91 0.06080731713245768 1103 92-93 0.05593439126835875 1103 94-95 0.12436047447120302 1103 96-97 0.15025033853719094 1103 98-99 0.19139392480531114 1103 100 0.21093307509468673 1104 1 0.05104073193054148 1104 2 0.06320900198135604 1104 3 0.08963947564314623 1104 4 0.06848794227197885 1104 5 -0.012457810471801167 1104 6 0.03668822970826113 1104 7 0.05035576435316358 1104 8 0.042712279364231165 1104 9 0.0897040034583938 1104 10-11 0.08135400765270617 1104 12-13 0.0994551364584737 1104 14-15 0.15877456987147553 1104 16-17 0.16388250348028066 1104 18-19 0.12778775748639504 1104 20-21 0.10275404952221123 1104 22-23 0.09264017348969844 1104 24-25 0.08062199184735164 1104 26-27 0.027463580670527676 1104 28-29 0.10119210590779915 1104 30-31 0.054210768112419316 1104 32-33 0.12933491965377897 1104 34-35 0.07142107901543682 1104 36-37 0.049874594475156186 1104 38-39 0.059247232258961446 1104 40-41 0.11445651404880408 1104 42-43 0.1397213406104143 1104 44-45 0.18535355173708723 1104 46-47 0.1384761275609776 1104 48-49 0.1480120086884682 1104 50-51 0.18918128701891845 1104 52-53 0.22874033834676766 1104 54-55 0.20779807078332624 1104 56-57 0.23143930752114983 1104 58-59 0.19398039088834196 1104 60-61 0.22265719847757737 1104 62-63 0.2075415888598684 1104 64-65 0.21821666713206156 1104 66-67 0.16990255668486043 1104 68-69 0.23661531173249983 1104 70-71 0.29294821504632296 1104 72-73 0.34863956661945394 1104 74-75 0.3081813636819959 1104 76-77 0.2162654645450388 1104 78-79 0.17260337808252757 1104 80-81 0.23926509884164204 1104 82-83 0.225014781367733 1104 84-85 0.23017761513102641 1104 86-87 0.27307120237643545 1104 88-89 0.24221586858080002 1104 90-91 0.23484085408972533 1104 92-93 0.23757967898492183 1104 94-95 0.14140639464186222 1104 96-97 0.15530893906829846 1104 98-99 0.18437796957130104 1104 100 0.19190596589783127 1105 1 0.0025707746934600095 1105 2 -0.012814892101900455 1105 3 0.03695048528292233 1105 4 0.05250705270660916 1105 5 0.0075915964040902395 1105 6 -0.025447500281345015 1105 7 -0.04322327456834074 1105 8 -0.026333769293685805 1105 9 3.89297362865193E-4 1105 10-11 -0.03662943077594605 1105 12-13 -0.021543087793176596 1105 14-15 -0.05268516775264942 1105 16-17 0.0028349931519429106 1105 18-19 -0.017701902101443068 1105 20-21 0.05429671580231599 1105 22-23 0.0849124065838538 1105 24-25 -0.004154046421838586 1105 26-27 -0.008748095780873655 1105 28-29 -0.004249535995029419 1105 30-31 0.02780061856299909 1105 32-33 0.00994640524242385 1105 34-35 0.1318734116481366 1105 36-37 0.09068328477015086 1105 38-39 0.06166176681961133 1105 40-41 0.1715050270105607 1105 42-43 0.1614471988887871 1105 44-45 0.1712289835942542 1105 46-47 0.18576406455211014 1105 48-49 0.091151761291691 1105 50-51 0.09571906093112403 1105 52-53 0.16535234337200677 1105 54-55 0.20305839307561513 1105 56-57 0.1724923142134287 1105 58-59 0.0795270073025307 1105 60-61 0.04799676505001571 1105 62-63 0.05279641478821162 1105 64-65 -0.02912393831356752 1105 66-67 -0.014093239502713573 1105 68-69 0.001961703460167996 1105 70-71 0.04943585728739208 1105 72-73 0.044002749436970134 1105 74-75 -0.015573981682940996 1105 76-77 -0.048883239279454926 1105 78-79 -0.07150741895064527 1105 80-81 0.0446416299716077 1105 82-83 0.052311476301412085 1105 84-85 0.026657949020034266 1105 86-87 0.0213120639163904 1105 88-89 0.005965234384959217 1105 90-91 -0.019311497870845074 1105 92-93 0.06747905324503023 1105 94-95 -0.01633411658038142 1105 96-97 -0.08332409709898059 1105 98-99 -0.12847778409495447 1105 100 -0.06900401453270177 1106 1 0.06472246376873869 1106 2 0.08839150268894969 1106 3 0.054093199042746676 1106 4 0.018489138615251477 1106 5 -0.01844670490471856 1106 6 -0.007042668920611561 1106 7 -0.008693055206173028 1106 8 5.35459828242324E-4 1106 9 0.016208551021797746 1106 10-11 0.025542580531890735 1106 12-13 0.038731815642975675 1106 14-15 0.06838607927736007 1106 16-17 -0.008604168963856296 1106 18-19 0.052171859543229004 1106 20-21 0.02951680297920234 1106 22-23 0.06284448557772748 1106 24-25 0.03148178333347573 1106 26-27 0.08712638694170494 1106 28-29 0.06710023258013109 1106 30-31 0.06826362897769656 1106 32-33 0.0984793007795659 1106 34-35 0.08865212791670274 1106 36-37 0.09242079229197486 1106 38-39 0.11656590601031525 1106 40-41 0.125871583888987 1106 42-43 0.14351894267162635 1106 44-45 0.1509572819937759 1106 46-47 0.18376011319318764 1106 48-49 0.10120218933629843 1106 50-51 0.009583841941292803 1106 52-53 0.0415564428779831 1106 54-55 0.04035588188364869 1106 56-57 0.013172312881359005 1106 58-59 -0.04286024159792845 1106 60-61 -0.04101472918158322 1106 62-63 -0.07717576774672352 1106 64-65 -0.08121944667811931 1106 66-67 -0.08381462776500825 1106 68-69 -0.14347260490863079 1106 70-71 -0.08256094573346218 1106 72-73 -0.0475546319910336 1106 74-75 -0.036913395459087894 1106 76-77 -0.08699710946046224 1106 78-79 -0.10384249030220616 1106 80-81 -0.14071135362121368 1106 82-83 -0.12593193466997832 1106 84-85 -0.06850690331162923 1106 86-87 -0.07379612899022447 1106 88-89 -0.13427817450507007 1106 90-91 -0.14954283945964164 1106 92-93 -0.09207186415201107 1106 94-95 -0.12233068995711704 1106 96-97 -0.0398779968631402 1106 98-99 -0.07281351246459167 1106 100 -0.02310230637822741 1107 1 -0.03951136221968454 1107 2 -0.024080140743926393 1107 3 4.0998661489766164E-5 1107 4 0.016644167032559665 1107 5 -0.1339208311248541 1107 6 -0.09788197912985908 1107 7 -0.029358669707065133 1107 8 -0.03542260006347675 1107 9 -0.05195400969494557 1107 10-11 -0.05216018410915524 1107 12-13 1.9068509121211719E-4 1107 14-15 0.007859028161540493 1107 16-17 0.015061111995336773 1107 18-19 0.0342548164456673 1107 20-21 -0.0126082442357216 1107 22-23 -0.010181737206039543 1107 24-25 -0.008586211683279998 1107 26-27 0.025282895312273013 1107 28-29 -0.010929447109390367 1107 30-31 0.01691441412603467 1107 32-33 0.007157984151298535 1107 34-35 0.04617330008385778 1107 36-37 0.02085936429170232 1107 38-39 0.019467608398336722 1107 40-41 0.016536530202024835 1107 42-43 0.11476143522216375 1107 44-45 0.1286978461873005 1107 46-47 0.06939109237002583 1107 48-49 0.10620630890769434 1107 50-51 0.055174133447906115 1107 52-53 0.05053738461421631 1107 54-55 0.11354429208250849 1107 56-57 0.1142029512271634 1107 58-59 0.07498967899263675 1107 60-61 0.06185588314355073 1107 62-63 0.06950910933984744 1107 64-65 5.793647206147057E-4 1107 66-67 -8.070314079020591E-4 1107 68-69 0.007251633412437286 1107 70-71 0.029304220584563723 1107 72-73 0.009720967334814645 1107 74-75 -0.04362462454922422 1107 76-77 -0.02610675190816636 1107 78-79 0.012779621303941724 1107 80-81 0.02684644186690832 1107 82-83 0.08371156226077403 1107 84-85 0.03880199496256864 1107 86-87 0.0020676832810195833 1107 88-89 0.05074823272063611 1107 90-91 -0.01367247232079194 1107 92-93 -9.004756245118983E-4 1107 94-95 -0.06584236502122565 1107 96-97 -0.06605312392927942 1107 98-99 -0.054828214806221354 1107 100 0.02414895655203253 1108 1 -0.015688113703411943 1108 2 -0.02997924709497113 1108 3 -0.08760268440011032 1108 4 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2214 7 -0.05958381839015203 2214 8 -0.01964095180519365 2214 9 -0.06100243790225335 2214 10-11 -0.09645712548553576 2214 12-13 -0.07364109060971913 2214 14-15 -0.0850679047186631 2214 16-17 -0.0816253790836825 2214 18-19 -0.07122521753904465 2214 20-21 -0.08930747957982277 2214 22-23 -0.12193832174300212 2214 24-25 -0.13595452094411797 2214 26-27 -0.13085559236826327 2214 28-29 -0.0849630070074312 2214 30-31 -0.07810470227158106 2214 32-33 -0.07603020616306111 2214 34-35 -0.11853570586346507 2214 36-37 -0.10184666799376885 2214 38-39 -0.1318280585515339 2214 40-41 -0.1118938011488595 2214 42-43 -0.12078444413212708 2214 44-45 -0.12334123622561322 2214 46-47 -0.10388843184408358 2214 48-49 -0.1392907174304625 2214 50-51 0.015560709488688929 2214 52-53 -0.05137425514789129 2214 54-55 -0.042902182004148415 2214 56-57 -0.07996654749840815 2214 58-59 -0.10983402534040465 2214 60-61 -0.14239220521668727 2214 62-63 -0.12228145990398787 2214 64-65 -0.02870058430232092 2214 66-67 -0.15040346301368857 2214 68-69 -0.13202194347796592 2214 70-71 -0.09167071569410723 2214 72-73 -0.19037461465329386 2214 74-75 -0.08320618784574663 2214 76-77 -0.07111524808488667 2214 78-79 -0.2645674475150912 2214 80-81 -0.12949383001074466 2214 82-83 -0.10165377333819947 2214 84-85 -0.13414830240361653 2214 86-87 -0.06279000575892013 2214 88-89 -0.12488898053339881 2214 90-91 -0.010656329585025048 2214 92-93 -0.06225349176724393 2214 94-95 -0.12317031744264284 2214 96-97 -0.1931346872257933 2214 98-99 -0.20988343995490766 2214 100 -0.40783914880055505 2215 1 -0.04897667532144112 2215 2 -0.11939224242993163 2215 3 -0.13467472525519497 2215 4 -0.09255514585741764 2215 5 -0.10558028810400089 2215 6 -0.09749567583666874 2215 7 -0.10551032414405626 2215 8 -0.13046801521205964 2215 9 -0.1393132639138841 2215 10-11 -0.14763901390431755 2215 12-13 -0.15896950723684 2215 14-15 -0.21224903871402745 2215 16-17 -0.24327980360229873 2215 18-19 -0.21950997387204296 2215 20-21 -0.21558640731453949 2215 22-23 -0.204073586010459 2215 24-25 -0.17702616407188998 2215 26-27 -0.13477589338178575 2215 28-29 -0.1725259484173307 2215 30-31 -0.19071336010028261 2215 32-33 -0.18727802284530526 2215 34-35 -0.1979957262357459 2215 36-37 -0.12330735793466374 2215 38-39 -0.15729007158565622 2215 40-41 -0.1678090190741912 2215 42-43 -0.11328923325901741 2215 44-45 -0.1452101116605391 2215 46-47 -0.22742063085641462 2215 48-49 -0.16297590466900402 2215 50-51 -0.15976345013675086 2215 52-53 -0.111836380489855 2215 54-55 -0.1968780040885747 2215 56-57 -0.19581029485783574 2215 58-59 -0.18514681156940327 2215 60-61 -0.15758821269103862 2215 62-63 -0.16379685267300204 2215 64-65 -0.1570010530360051 2215 66-67 -0.18648792527842772 2215 68-69 -0.18370288868921136 2215 70-71 -0.1775755567321724 2215 72-73 -0.1052906903965507 2215 74-75 -0.1588244335545994 2215 76-77 -0.17772804918130447 2215 78-79 -0.13291187139551397 2215 80-81 -0.05910079537821389 2215 82-83 -0.07871534405773417 2215 84-85 -0.1085356988263797 2215 86-87 -0.12084299437731971 2215 88-89 -0.15830479475984305 2215 90-91 -0.07215810458359329 2215 92-93 -0.09417757274155036 2215 94-95 -0.10903940715378013 2215 96-97 -0.11523021947757428 2215 98-99 -0.08721298072995864 2215 100 -0.04271426070729234 2216 1 -0.0870200254560558 2216 2 -0.08437814107395525 2216 3 -0.11397356757663601 2216 4 -0.044664169176016344 2216 5 -0.04714520519664944 2216 6 -0.09555678148805669 2216 7 -0.07059304962935187 2216 8 -0.09305412374299493 2216 9 -0.10999131786613958 2216 10-11 -0.10094208057035559 2216 12-13 -0.13237858329659957 2216 14-15 -0.1657263515957439 2216 16-17 -0.18338831870627814 2216 18-19 -0.15723195993032135 2216 20-21 -0.1045746863833088 2216 22-23 -0.16318424241897844 2216 24-25 -0.24265560758193772 2216 26-27 -0.2433563765258313 2216 28-29 -0.2548019887754265 2216 30-31 -0.24322007230531284 2216 32-33 -0.21678461346950684 2216 34-35 -0.19394592255006415 2216 36-37 -0.19866592035749875 2216 38-39 -0.2118985577257817 2216 40-41 -0.26316674457633127 2216 42-43 -0.23805861698227204 2216 44-45 -0.12418071099006056 2216 46-47 -0.10985314339001917 2216 48-49 -0.11533777233920972 2216 50-51 -0.029712456912271534 2216 52-53 -0.051321835919530656 2216 54-55 -0.014851246538270857 2216 56-57 0.013737724182867339 2216 58-59 0.040008768115214366 2216 60-61 0.009770330871603505 2216 62-63 0.02627318428622516 2216 64-65 0.05098721188762312 2216 66-67 0.08108530444064144 2216 68-69 0.07903986041173283 2216 70-71 0.06938050206849766 2216 72-73 0.028341675094971208 2216 74-75 0.02386592464745263 2216 76-77 0.03399979464828817 2216 78-79 0.011055412246172125 2216 80-81 -0.017376004541034717 2216 82-83 0.013821184078629756 2216 84-85 0.03576517433281623 2216 86-87 -0.005481895933215242 2216 88-89 -0.021228635313150335 2216 90-91 -0.015372866854328038 2216 92-93 8.122949609870034E-4 2216 94-95 -0.03563148459639365 2216 96-97 -0.05524245502242309 2216 98-99 -0.04540647645846718 2216 100 -0.05464299076349022 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19.0 3 7.0 4 4.0 5 23.0 6 50.0 7 130.0 8 278.0 9 552.0 10 1018.0 11 1722.0 12 2649.0 13 4257.0 14 5112.0 15 6886.0 16 8130.0 17 11343.0 18 13096.0 19 7248.0 20 4902.0 21 4464.0 22 4749.0 23 5009.0 24 5791.0 25 7141.0 26 8666.0 27 11248.0 28 14451.0 29 18860.0 30 24457.0 31 31575.0 32 40835.0 33 53535.0 34 74488.0 35 113605.0 36 208558.0 37 503892.0 38 1012242.0 39 559175.0 40 2704.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.77553192142186 20.15256902985142 13.32410685246691 23.74779219625981 2 15.537413680434039 20.367919343228376 40.5549769261507 23.539690050186884 3 17.99337221241089 25.462634215583773 30.550609819209047 25.99338375279629 4 11.909595310265736 16.761436276198083 39.729160994134226 31.599807419401955 5 11.995102184535371 38.23153361944811 36.495399459133395 13.277964736883119 6 30.545652043769877 38.985295358665006 17.550721888472072 12.918330709093045 7 26.641860995191426 32.934776375681494 22.883069642337418 17.540292986789662 8 24.842058494075797 36.666664262411906 20.470221742012402 18.021055501499895 9 24.878356993553243 16.07011224479492 21.609935994068234 37.441594767583595 10-11 23.480426426375526 26.742060584772 29.543718152224503 20.23379483662798 12-13 24.487651967942252 24.199701320400408 29.685693997304597 21.62695271435274 14-15 21.554357878853615 26.19251507960078 27.067385272687833 25.185741768857774 16-17 20.580834809841498 29.75780337419231 27.539074122092227 22.122287693873965 18-19 20.30368551555907 28.320257523932348 30.392371654119195 20.983685306389393 20-21 21.673042128537535 27.650835541934693 29.95950767273342 20.716614656794345 22-23 21.13539048657686 27.42582480694128 30.140025049939396 21.298759656542465 24-25 21.06580623383443 27.613431292077372 30.064665898268505 21.256096575819694 26-27 20.80773364300604 27.483482519600983 30.174515475220172 21.534268362172806 28-29 20.770141464307514 27.599777465797086 30.229756646045285 21.400324423850112 30-31 21.139297956108997 28.17036165033745 29.756899886392375 20.933440507161176 32-33 20.686365648277512 27.782767936343372 30.300080479834406 21.23078593554471 34-35 20.82737799339255 27.563670129602148 30.33621306035811 21.2727388166472 36-37 21.250269200342412 28.015810402153747 29.623473043350906 21.110447354152935 38-39 21.14367105925055 28.13909281818757 29.634240329969558 21.082995792592314 40-41 20.909030236673264 27.88126834213275 29.874781968747797 21.334919452446186 42-43 20.963767848011997 27.733842436988056 29.785828365093693 21.516561349906258 44-45 21.16829896344751 27.979865223466334 29.566428475966088 21.28540733712007 46-47 21.28151735625546 28.28351670627734 29.203405279395007 21.23156065807219 48-49 21.209783354360937 28.148171391926407 29.08631633163165 21.555728922081006 50-51 21.278722143456623 28.08425029836153 28.947451943867275 21.689575614314574 52-53 21.270407388586396 28.100406497760854 28.95341703727293 21.675769076379815 54-55 21.426866059208262 28.508288687604928 28.739088018955876 21.325757234230935 56-57 21.200333526159156 28.201551791044132 28.83930155698436 21.758813125812356 58-59 21.01565451337391 27.79836936284697 29.328946739672375 21.857029384106745 60-61 21.36625681811174 28.196026223527497 28.850983292121274 21.58673366623949 62-63 21.159319257558764 28.195914239825044 28.877926144288562 21.766840358327634 64-65 21.187436582525983 27.83182970694027 29.065493987748468 21.91523972278528 66-67 21.0469249372668 28.022896265003496 28.840735934888745 22.089442862840958 68-69 21.638281789271495 28.131214301015785 28.71172062702545 21.518783282687266 70-71 21.518327921050453 27.958899468220444 28.74440627085505 21.778366339874054 72-73 21.326012119996875 28.10196925155314 28.84023347051442 21.731785157935565 74-75 21.399907063314334 28.150330949449042 28.6702462497595 21.779515737477123 76-77 21.350991638775785 28.193927604337688 28.5425749122882 21.91250584459833 78-79 21.526508910715055 28.27871993254641 28.63126086532381 21.563510291414726 80-81 21.48364371806759 28.022622219962212 28.693556699264906 21.800177362705288 82-83 21.215325437257064 28.17061410373572 28.8275906340543 21.78646982495292 84-85 21.2185782742563 28.07283087423195 28.647201222563325 22.061389628948426 86-87 21.3866730715898 28.21884352909822 28.51882185478063 21.875661544531347 88-89 21.180700436479015 28.075557788299566 28.57810550869478 22.165636266526644 90-91 21.126197766213345 27.950931706138405 28.71259741711656 22.210273110531688 92-93 21.2658004161753 28.192450421404537 28.459736662735725 22.082012499684442 94-95 20.97091786815903 27.962912807700036 28.658653792405055 22.407515531735882 96-97 20.952795856713134 27.935522424231056 28.68418689509898 22.42749482395683 98-99 21.077756592354998 28.011995509347532 28.50240779322226 22.40784010507521 100 21.134448735624556 27.98056599098912 28.4061177025545 22.47886757083182 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 46.0 1 37.0 2 95.5 3 165.5 4 122.0 5 94.5 6 229.5 7 552.0 8 941.0 9 1137.0 10 998.5 11 793.5 12 829.0 13 989.5 14 1174.5 15 1335.0 16 1547.0 17 1852.5 18 2183.0 19 2576.0 20 3252.5 21 4251.5 22 5582.0 23 8111.5 24 11290.5 25 14759.0 26 19633.5 27 25714.0 28 33261.0 29 41839.0 30 50137.5 31 58838.5 32 67463.5 33 75491.5 34 83473.5 35 90283.0 36 95842.5 37 100441.0 38 102564.0 39 103028.5 40 103761.5 41 103792.0 42 101648.5 43 99133.0 44 97033.0 45 94635.5 46 92124.0 47 89164.5 48 86283.0 49 84135.5 50 81545.5 51 77625.0 52 72338.0 53 67823.0 54 64774.5 55 61272.5 56 56964.0 57 51931.5 58 46775.0 59 41798.5 60 36649.0 61 30929.0 62 25935.5 63 21576.5 64 17477.5 65 13748.5 66 10477.0 67 7790.0 68 5718.5 69 4295.5 70 3020.0 71 2140.0 72 1626.5 73 1262.5 74 935.0 75 665.0 76 435.5 77 282.5 78 187.0 79 106.5 80 65.0 81 29.0 82 12.5 83 5.0 84 3.5 85 3.0 86 1.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09318861209194369 2 0.0012622296529481538 3 0.0 4 2.163822262196835E-4 5 0.03689316957045604 6 1.8031852184973625E-4 7 0.004255517115653776 8 3.2457333932952524E-4 9 1.0819111310984175E-4 10-11 4.5079630462434056E-4 12-13 0.0 14-15 9.015926092486813E-5 16-17 0.0 18-19 9.015926092486813E-5 20-21 0.0 22-23 3.606370436994725E-4 24-25 1.0819111310984175E-4 26-27 0.0018392489228673098 28-29 0.0095027861014811 30-31 0.03786688958844461 32-33 0.026975650868720544 34-35 0.030618085010085214 36-37 0.027841179773599276 38-39 0.026560918268466148 40-41 0.03267371615917221 42-43 0.02446922341500921 44-45 0.02353156710139058 46-47 0.016949941053875206 48-49 0.011720703920232856 50-51 0.008222524596347973 52-53 0.005842320107931454 54-55 0.004183389706913881 56-57 0.002704777827746044 58-59 0.0010278155745434966 60-61 6.311148264740769E-4 62-63 0.0016408985488325998 64-65 0.0026867459755610702 66-67 6.130829742891032E-4 68-69 0.0011901022442082593 70-71 4.1473260025439335E-4 72-73 5.589874177341824E-4 74-75 5.589874177341824E-4 76-77 4.1473260025439335E-4 78-79 5.409555655492087E-5 80-81 7.934014961388394E-4 82-83 6.130829742891032E-4 84-85 3.065414871445516E-4 86-87 1.44254817479789E-4 88-89 0.0 90-91 3.606370436994725E-5 92-93 3.606370436994725E-5 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2772871.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.23456204253304 #Duplication Level Percentage of deduplicated Percentage of total 1 78.68308882729752 54.475891951116694 2 13.860832939886473 19.192973962755115 3 3.8771809079141093 8.05304766357312 4 1.4901683609332321 4.126846153554065 5 0.7086525122213723 2.4531623161993754 6 0.3904913941733864 1.622130039418353 7 0.2328047627878813 1.128269505512433 8 0.14937541712443994 0.8273553267625032 9 0.11318721817203427 0.7052820731058087 >10 0.46983850793039056 5.4507747627187415 >50 0.018351974133960922 0.8585353294028806 >100 0.005714583513157279 0.6746162484714463 >500 1.0419797084152815E-4 0.037477254053605774 >1k 2.0839594115678141E-4 0.39363741335581803 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4243 0.15301829764168617 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3689 0.1330390054207354 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.6063704369947246E-5 0.0 6 0.0 0.0 0.0 3.6063704369947246E-5 0.0 7 0.0 0.0 0.0 3.6063704369947246E-5 0.0 8 0.0 0.0 0.0 3.6063704369947246E-5 0.0 9 0.0 0.0 0.0 7.212740873989449E-5 0.0 10-11 0.0 0.0 0.0 7.212740873989449E-5 0.0 12-13 0.0 0.0 0.0 7.212740873989449E-5 0.0 14-15 0.0 0.0 0.0 1.0819111310984175E-4 0.0 16-17 0.0 0.0 0.0 1.2622296529481538E-4 0.0 18-19 0.0 0.0 0.0 1.8031852184973625E-4 0.0 20-21 0.0 0.0 0.0 2.163822262196835E-4 0.0 22-23 0.0 0.0 0.0 2.5244593058963076E-4 0.0 24-25 0.0 0.0 0.0 3.2457333932952524E-4 0.0 26-27 0.0 0.0 0.0 4.5079630462434067E-4 0.0 28-29 0.0 0.0 0.0 0.0012622296529481538 0.0 30-31 0.0 0.0 0.0 0.004922695646497799 0.0 32-33 0.0 0.0 0.0 0.012838678755701221 0.0 34-35 0.0 0.0 0.0 0.02363975821450042 0.0 36-37 0.0 0.0 0.0 0.04338463635704654 0.0 38-39 0.0 0.0 0.0 0.07649111696865812 0.0 40-41 0.0 0.0 0.0 0.11857745996838656 0.0 42-43 0.0 0.0 0.0 0.16028513407223055 0.0 44-45 0.0 0.0 0.0 0.20738433197938166 0.0 46-47 0.0 0.0 0.0 0.26633045677206046 0.0 48-49 0.0 0.0 0.0 0.33257948169965357 0.0 50-51 0.0 0.0 0.0 0.4076100186413288 0.0 52-53 0.0 0.0 0.0 0.48312741559199834 0.0 54-55 0.0 0.0 0.0 0.5625036289102523 0.0 56-57 0.0 0.0 0.0 0.6534202276268892 0.0 58-59 0.0 0.0 0.0 0.7613228311017715 0.0 60-61 0.0 0.0 0.0 0.8752661050586197 0.0 62-63 0.0 0.0 0.0 0.992671494634983 0.0 64-65 0.0 0.0 0.0 1.1197419569824922 0.0 66-67 0.0 0.0 0.0 1.261977207017564 0.0 68-69 0.0 0.0 0.0 1.4205312832800372 0.0 70-71 0.0 0.0 0.0 1.593510841290489 0.0 72-73 0.0 0.0 0.0 1.7818355055103536 0.0 74-75 0.0 0.0 0.0 1.9671488504153278 0.0 76-77 0.0 0.0 0.0 2.1621272680914476 0.0 78-79 0.0 0.0 0.0 2.3949184797994567 0.0 80-81 0.0 0.0 0.0 2.6470037733453884 0.0 82-83 0.0 3.6063704369947246E-5 0.0 2.913226039004339 0.0 84-85 0.0 3.6063704369947246E-5 0.0 3.1754272016260403 0.0 86-87 0.0 3.6063704369947246E-5 0.0 3.45266692897001 0.0 88 0.0 3.6063704369947246E-5 0.0 3.6773077434904113 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3670 0.0 51.664223 1 GTATCAA 6625 0.0 41.61622 1 TCAACGC 7825 0.0 34.96274 4 ATCAACG 7915 0.0 34.624577 3 CAACGCA 7945 0.0 34.435295 5 AACGCAG 8200 0.0 33.41152 6 TATCAAC 8560 0.0 32.0156 2 ACGCAGA 9430 0.0 28.903992 7 CGCAGAG 9610 0.0 28.508795 8 GTACATG 6900 0.0 25.50195 1 GCAGAGT 10815 0.0 25.028202 9 TACATGG 6780 0.0 24.266394 2 ACATGGG 7115 0.0 22.198889 3 GAGTACT 7330 0.0 21.765348 12-13 AGAGTAC 9940 0.0 21.061779 10-11 CAGAGTA 10845 0.0 20.322493 10-11 GTCGTAT 120 0.005035435 19.603773 1 GTGGTAT 1950 0.0 18.819622 1 AGTACTT 8190 0.0 18.762627 12-13 GTACTTT 8680 0.0 18.407253 14-15 >>END_MODULE