FastQCFastQC Report
Wed 25 May 2016
SRR1033180_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033180_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2660110
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21750.035.5196271
TCAACGC33100.032.2135474
GTATCAA35650.032.109281
ATCAACG33600.031.6003273
CAACGCA34750.030.8191575
AACGCAG36850.028.9266686
GTACATG73050.026.0522351
TATCAAC42600.025.600832
TACATGG73700.024.6842042
ACGCAGA43300.024.0759417
ACATGGG76900.022.7268353
CGCAGAG45300.022.7020028
CATGGGG50150.018.8263364
GTATAGG9550.018.250811
GCAGAGT54900.018.1335059
GTATTAG14050.017.7698331
GAGTACA54250.017.5402361
TAGACGG2705.4854536E-717.3971045
CTAGACG2203.2133823E-517.0807914
CTACACT13950.016.8359074
Produced by FastQC (version 0.11.5)