##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033180_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2660110
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.652470762487265	33.0	31.0	34.0	30.0	34.0
2	31.739347996887346	34.0	31.0	34.0	30.0	34.0
3	31.823654284973177	34.0	31.0	34.0	30.0	34.0
4	35.1886121250625	37.0	35.0	37.0	33.0	37.0
5	35.26289439158531	37.0	35.0	37.0	33.0	37.0
6	35.35221889320366	37.0	36.0	37.0	33.0	37.0
7	35.260559901658205	37.0	36.0	37.0	33.0	37.0
8	35.28389953798903	37.0	36.0	37.0	33.0	37.0
9	36.94182909729297	39.0	38.0	39.0	34.0	39.0
10-11	36.94005943363244	39.0	38.0	39.0	34.0	39.0
12-13	36.91982399224092	39.0	38.0	39.0	34.0	39.0
14-15	38.3850346414246	41.0	39.0	41.0	34.0	41.0
16-17	38.402427343230165	41.0	39.0	41.0	34.0	41.0
18-19	38.39507783512712	41.0	39.0	41.0	34.0	41.0
20-21	38.42260244877092	41.0	39.0	41.0	34.0	41.0
22-23	38.39546071403062	41.0	39.0	41.0	34.0	41.0
24-25	38.39224543345952	41.0	39.0	41.0	34.0	41.0
26-27	38.34940077665961	40.5	39.0	41.0	34.0	41.0
28-29	38.32330148001398	40.0	39.0	41.0	34.0	41.0
30-31	38.3266532211074	40.5	39.0	41.0	34.0	41.0
32-33	38.39048479198229	41.0	39.0	41.0	34.0	41.0
34-35	38.36262391404867	41.0	39.0	41.0	34.0	41.0
36-37	38.28837096962155	41.0	39.0	41.0	34.0	41.0
38-39	38.21191924394104	41.0	39.0	41.0	33.0	41.0
40-41	38.15303746837537	41.0	38.5	41.0	33.0	41.0
42-43	38.04781118074065	40.5	38.0	41.0	33.0	41.0
44-45	37.93310934510227	40.0	38.0	41.0	33.0	41.0
46-47	37.814000360887334	40.0	38.0	41.0	33.0	41.0
48-49	37.6987844487634	40.0	38.0	41.0	32.5	41.0
50-51	37.58166166060802	40.0	38.0	41.0	32.0	41.0
52-53	37.439507388792194	40.0	37.0	41.0	32.0	41.0
54-55	37.29292980365474	40.0	37.0	41.0	31.5	41.0
56-57	37.1434008368075	40.0	37.0	41.0	31.0	41.0
58-59	36.95519226648522	40.0	36.0	41.0	31.0	41.0
60-61	36.74193623571958	39.5	36.0	41.0	31.0	41.0
62-63	36.489193304036306	39.0	35.0	41.0	31.0	41.0
64-65	36.229300480055336	39.0	35.0	41.0	30.5	41.0
66-67	35.94739484457409	38.5	35.0	40.5	30.0	41.0
68-69	35.64469627195868	37.5	35.0	40.0	30.0	41.0
70-71	35.3074079643323	37.0	35.0	40.0	30.0	41.0
72-73	34.943501584520945	36.5	35.0	39.0	29.0	41.0
74-75	34.57450481371071	36.0	34.0	39.0	29.0	41.0
76-77	34.24102311558545	36.0	34.0	39.0	29.0	40.0
78-79	33.869300329685615	35.0	34.0	37.5	29.0	39.5
80-81	33.50366150271981	35.0	34.0	37.0	28.0	39.0
82-83	33.26928548067561	35.0	34.0	37.0	29.0	39.0
84-85	32.98717910161609	35.0	34.0	36.0	28.0	37.5
86-87	32.72434354218434	35.0	34.0	36.0	28.0	37.0
88-89	32.355439060790715	35.0	34.0	35.5	27.0	37.0
90-91	32.1571173748454	35.0	34.0	35.0	27.0	36.0
92-93	31.97489784257042	35.0	34.0	35.0	26.5	36.0
94-95	31.800423666690477	35.0	33.0	35.0	26.0	36.0
96-97	31.625282601095442	35.0	33.0	35.0	25.5	36.0
98-99	31.434481280849287	35.0	33.0	35.0	25.0	35.5
100	31.274218359391153	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.04431409485729887
1101	2	0.07232561381103153
1101	3	0.06738776202490371
1101	4	0.018814440054782722
1101	5	0.026632630175491556
1101	6	-0.02290225489215203
1101	7	0.02548887566617708
1101	8	0.0019723521158141466
1101	9	-0.03144608571855656
1101	10-11	-0.021961107429667948
1101	12-13	0.014241904338597067
1101	14-15	0.026721737576053783
1101	16-17	-0.04018694502705955
1101	18-19	0.0257313963933683
1101	20-21	0.03138560307475302
1101	22-23	-0.016413066270303034
1101	24-25	-0.0022548699202857847
1101	26-27	0.07711549326403855
1101	28-29	0.0861047642637871
1101	30-31	0.13159029857020244
1101	32-33	0.10087066000531308
1101	34-35	0.10891279988670277
1101	36-37	0.20142122255445827
1101	38-39	0.11867025414876764
1101	40-41	0.08673183267737272
1101	42-43	0.08572986233659918
1101	44-45	0.11184607890660914
1101	46-47	0.1955808956583951
1101	48-49	0.17105667075701092
1101	50-51	0.11905335577719711
1101	52-53	0.09917093936541477
1101	54-55	0.10808761460585714
1101	56-57	0.16299425325402694
1101	58-59	0.0485510935655995
1101	60-61	0.03159625363540641
1101	62-63	-0.010735845150058765
1101	64-65	0.04685089793613173
1101	66-67	0.016457999775603582
1101	68-69	0.04474908814184886
1101	70-71	0.09568499693206434
1101	72-73	0.06987662173112597
1101	74-75	-0.006423368752727754
1101	76-77	-0.0692851007394566
1101	78-79	-0.07369606962091524
1101	80-81	0.03015127069814838
1101	82-83	-0.027041069281267482
1101	84-85	-0.05327575850442656
1101	86-87	-0.01204889374321283
1101	88-89	0.17836172973860442
1101	90-91	0.23386669649575964
1101	92-93	0.23700944539398705
1101	94-95	0.2569217797069818
1101	96-97	0.16993277613791946
1101	98-99	0.23216265961380245
1101	100	0.19115444025831962
1102	1	0.13821180847157777
1102	2	0.15068129350839854
1102	3	0.12263410570026068
1102	4	0.12467195788380536
1102	5	-0.005721224485370158
1102	6	0.0411523979521391
1102	7	0.07547392350642923
1102	8	0.04054189578714329
1102	9	0.13465383909893802
1102	10-11	0.09447079084652188
1102	12-13	0.13272645078949807
1102	14-15	0.16816602446753137
1102	16-17	0.21651433775134876
1102	18-19	0.21550545083758266
1102	20-21	0.14352077148667064
1102	22-23	0.12780321490782853
1102	24-25	0.08442463493757657
1102	26-27	0.1753994203507645
1102	28-29	0.2514594855728376
1102	30-31	0.28291122311529193
1102	32-33	0.27838298166511777
1102	34-35	0.33895292435141045
1102	36-37	0.27838445000736556
1102	38-39	0.1567878667571705
1102	40-41	0.207637245507172
1102	42-43	0.2135464874514028
1102	44-45	0.1719718131789847
1102	46-47	0.23651404211808824
1102	48-49	0.23530667826638307
1102	50-51	0.26050774148018263
1102	52-53	0.24614745137944993
1102	54-55	0.2616995266756206
1102	56-57	0.14269168398320886
1102	58-59	0.11360552874906205
1102	60-61	0.22595305996062365
1102	62-63	0.2791131402560083
1102	64-65	0.277855329427787
1102	66-67	0.15267369174542722
1102	68-69	0.20608995728477453
1102	70-71	0.2368355304822387
1102	72-73	0.2980565303194993
1102	74-75	0.27459078423220973
1102	76-77	0.20033677904221747
1102	78-79	0.2693035896089313
1102	80-81	0.19721546555060598
1102	82-83	0.1744228958657743
1102	84-85	0.2211897406261869
1102	86-87	0.1952740837089877
1102	88-89	0.3519853801045798
1102	90-91	0.385034104309149
1102	92-93	0.44226183355944926
1102	94-95	0.4537276403025423
1102	96-97	0.46281832249693977
1102	98-99	0.4388860340047849
1102	100	0.4465825329472075
1103	1	0.09071445514872778
1103	2	0.10832157813156584
1103	3	0.09339435217291836
1103	4	0.13692009418943485
1103	5	0.05357543564206679
1103	6	0.1057458473027495
1103	7	0.16458897800071526
1103	8	0.08598115975256349
1103	9	0.11141489020152306
1103	10-11	0.18247189374196893
1103	12-13	0.1933680966427005
1103	14-15	0.23349794576264316
1103	16-17	0.17716618817834728
1103	18-19	0.23000074020308148
1103	20-21	0.24105527919040526
1103	22-23	0.23665617644546444
1103	24-25	0.18859182936532903
1103	26-27	0.30388545232157327
1103	28-29	0.3508797999452682
1103	30-31	0.27075697461344816
1103	32-33	0.19538902554936044
1103	34-35	0.16292016208777937
1103	36-37	0.20136965137166385
1103	38-39	0.21298161368632407
1103	40-41	0.24588418130838363
1103	42-43	0.19796390289477017
1103	44-45	0.18138163244066874
1103	46-47	0.22746739404865224
1103	48-49	0.3253337754887724
1103	50-51	0.2564826239164759
1103	52-53	0.14385733319960536
1103	54-55	0.18290239832569455
1103	56-57	0.17059793729736583
1103	58-59	0.23432967994105525
1103	60-61	0.2209098823050013
1103	62-63	0.23805500896393283
1103	64-65	0.1524352852690356
1103	66-67	0.26204740141428573
1103	68-69	0.2554949791086827
1103	70-71	0.23518115152501196
1103	72-73	0.27632955433544737
1103	74-75	0.20244215754886596
1103	76-77	0.13772184341046767
1103	78-79	0.18913209981415235
1103	80-81	0.21270777612516412
1103	82-83	0.1609868140671722
1103	84-85	0.1847424164786844
1103	86-87	0.16268660739741136
1103	88-89	0.24001598037253302
1103	90-91	0.2763954674455604
1103	92-93	0.20925960501219976
1103	94-95	0.25016725305542664
1103	96-97	0.33410411883225066
1103	98-99	0.38497593615680614
1103	100	0.38666923318162816
1104	1	0.10698898358267428
1104	2	0.06124840061153236
1104	3	0.024354770362297273
1104	4	0.014827746091498284
1104	5	0.003159180801780792
1104	6	-0.07119142305649007
1104	7	0.06675102707053782
1104	8	-0.10571090887241752
1104	9	-0.04035726914248272
1104	10-11	0.0049080234638196885
1104	12-13	-0.02384884410187027
1104	14-15	-0.10210278055992461
1104	16-17	-0.041887124605828774
1104	18-19	-0.06215262880748185
1104	20-21	-0.06363965120802106
1104	22-23	-0.10601867478632698
1104	24-25	-0.04784939980564218
1104	26-27	-0.071436005330618
1104	28-29	0.07100177047639278
1104	30-31	0.05664982859801171
1104	32-33	0.04488932916633814
1104	34-35	0.0204901376105866
1104	36-37	-0.0015454487095212244
1104	38-39	5.553294084137406E-4
1104	40-41	0.01739060141255777
1104	42-43	-0.04218396933903534
1104	44-45	-0.03528065375066092
1104	46-47	-0.0010809932029189895
1104	48-49	-0.07848435375622387
1104	50-51	-0.08528223326425888
1104	52-53	-0.11148311062549254
1104	54-55	-0.07368315619655164
1104	56-57	-0.08275178957074303
1104	58-59	-0.1075687166130237
1104	60-61	-0.15086921724750368
1104	62-63	-0.15330251885207957
1104	64-65	-0.17455530486668636
1104	66-67	-0.12613599558333277
1104	68-69	-0.15975571090187657
1104	70-71	-0.13793104526760658
1104	72-73	-0.152596684903763
1104	74-75	-0.16032817570590652
1104	76-77	-0.15715838863544462
1104	78-79	-0.18288333289523706
1104	80-81	-0.12751733369177742
1104	82-83	-0.16945345181053995
1104	84-85	-0.1110949048695602
1104	86-87	-0.03374484076854145
1104	88-89	-0.0320978793554616
1104	90-91	-0.10062724617596075
1104	92-93	-0.17929818895025562
1104	94-95	-0.16354420373163236
1104	96-97	-0.10577495399867232
1104	98-99	-0.12849628772208987
1104	100	-0.09164398629773629
1105	1	0.05916095330503879
1105	2	0.06373175998318459
1105	3	0.032888177700080234
1105	4	-0.011233006089334197
1105	5	0.036785456454865084
1105	6	0.02350445877119256
1105	7	-0.03181276814586198
1105	8	-0.029522439091081765
1105	9	-0.06089007877229591
1105	10-11	-0.055115758411496074
1105	12-13	0.0022554717434530858
1105	14-15	0.025687261412002727
1105	16-17	0.0454569690075175
1105	18-19	0.04002178087523589
1105	20-21	0.0660395325320593
1105	22-23	0.13729673938162534
1105	24-25	0.0906727139073098
1105	26-27	0.13230196782165393
1105	28-29	0.174752605281995
1105	30-31	0.18712603312346232
1105	32-33	0.17392437654602588
1105	34-35	0.14229562528885964
1105	36-37	0.1375251786263476
1105	38-39	0.20561298853614574
1105	40-41	0.14290140431229048
1105	42-43	0.12678940927767712
1105	44-45	0.1260147195510939
1105	46-47	0.12059996100663284
1105	48-49	0.13238235374547713
1105	50-51	0.10238657593564682
1105	52-53	0.07894410502807858
1105	54-55	0.025921853407545825
1105	56-57	0.0903025462058693
1105	58-59	0.15443916462353258
1105	60-61	0.11705505744711786
1105	62-63	0.14574725324376203
1105	64-65	0.13782922919605056
1105	66-67	0.2549579535586801
1105	68-69	0.16105557690129046
1105	70-71	0.13283876320966925
1105	72-73	0.18086857970878611
1105	74-75	0.17743757664187143
1105	76-77	0.14100763048132592
1105	78-79	0.15366418445993446
1105	80-81	0.13186596023889052
1105	82-83	0.1795918980629807
1105	84-85	0.16911267681494735
1105	86-87	0.21780631216046942
1105	88-89	0.35638170792310575
1105	90-91	0.3184026861775351
1105	92-93	0.3358391801587146
1105	94-95	0.3325590709322306
1105	96-97	0.381552563689727
1105	98-99	0.3070776294771811
1105	100	0.3698509039774791
1106	1	0.045488108447521824
1106	2	0.08062945188538606
1106	3	0.07591962126896945
1106	4	0.048393472420492856
1106	5	-0.00424093933745695
1106	6	0.01036311971721915
1106	7	-0.05658128797862361
1106	8	-0.007041534212277156
1106	9	-0.0017003346943056385
1106	10-11	-0.023573705908155773
1106	12-13	-0.00431607125239708
1106	14-15	0.053134491882438795
1106	16-17	0.026363813116468293
1106	18-19	-0.03762807253485079
1106	20-21	-0.02899256963235075
1106	22-23	-0.025358667106758048
1106	24-25	0.038678387961049054
1106	26-27	0.038797999434244446
1106	28-29	0.09359424581374043
1106	30-31	0.05311036047164208
1106	32-33	0.11298398249802233
1106	34-35	0.07512215742966788
1106	36-37	0.10995216863954482
1106	38-39	0.03765911992070414
1106	40-41	0.05302538885014485
1106	42-43	0.06899568681105706
1106	44-45	0.08012528586026235
1106	46-47	0.09875122921791046
1106	48-49	0.08362837891623087
1106	50-51	0.1311549280623936
1106	52-53	-0.011746420149442827
1106	54-55	0.10504830593166758
1106	56-57	0.05755860054610906
1106	58-59	-0.01718873239972396
1106	60-61	0.03392571548798884
1106	62-63	0.0333125941739425
1106	64-65	0.02575705970465947
1106	66-67	0.013945838982273528
1106	68-69	0.037086106385018525
1106	70-71	0.03205748898455596
1106	72-73	-0.03966462062435738
1106	74-75	-0.010061468001559604
1106	76-77	-0.024626959933833348
1106	78-79	0.029567842591198712
1106	80-81	0.042057420246457866
1106	82-83	0.01394835459196031
1106	84-85	-0.05569113115990376
1106	86-87	-0.06308380501975108
1106	88-89	0.03732056466591871
1106	90-91	0.09982190712192107
1106	92-93	0.11971175677394541
1106	94-95	0.07038018538242241
1106	96-97	0.04647418514491619
1106	98-99	0.05486065631756887
1106	100	0.08652730254306817
1107	1	-0.02449600721069345
1107	2	-0.05713555111986679
1107	3	-0.05006627819039977
1107	4	-0.02783931218340996
1107	5	-0.04692612188261336
1107	6	-0.08852409627203173
1107	7	-0.09348605540903776
1107	8	-0.06820037925768929
1107	9	-0.04766709168630001
1107	10-11	-0.15835096856787345
1107	12-13	-0.12474471158083134
1107	14-15	-0.1654141828561464
1107	16-17	-0.15648903661016078
1107	18-19	-0.10343594029630765
1107	20-21	-0.12262012808225364
1107	22-23	-0.08567398615019073
1107	24-25	-0.09536846382462016
1107	26-27	-0.047696102514528604
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2216	4	0.07334917626335624
2216	5	0.10838353654844468
2216	6	0.11319634148603086
2216	7	0.16312134279813506
2216	8	0.08596149574053413
2216	9	0.0926757533183391
2216	10-11	0.08644310762809226
2216	12-13	0.025386774121237465
2216	14-15	0.03703721796090065
2216	16-17	0.008777753950298006
2216	18-19	-8.254315970575021E-4
2216	20-21	-0.018966034598896897
2216	22-23	0.06507217361917839
2216	24-25	0.03530210681120849
2216	26-27	-0.0717680011288806
2216	28-29	0.006105011208418887
2216	30-31	-0.002447142830689586
2216	32-33	-0.030577968906392528
2216	34-35	-0.06305599779729931
2216	36-37	-0.06709902202409523
2216	38-39	-0.05213017955492205
2216	40-41	-0.017537840052071374
2216	42-43	-0.039797665960094264
2216	44-45	0.04909199154219124
2216	46-47	0.10795319513216839
2216	48-49	0.08439963493108138
2216	50-51	0.09969220728939376
2216	52-53	0.12187230117892511
2216	54-55	0.06084770904208625
2216	56-57	0.005723112065446401
2216	58-59	-0.04471406581993165
2216	60-61	0.043188208064357525
2216	62-63	0.0863274088566115
2216	64-65	0.1253608569835194
2216	66-67	0.08987564460145592
2216	68-69	0.05625420994584829
2216	70-71	-0.013417037719442249
2216	72-73	0.07845751706048532
2216	74-75	0.0751096807665661
2216	76-77	0.041709305103132976
2216	78-79	0.055375573611854634
2216	80-81	0.09440233069363302
2216	82-83	0.13304900233842432
2216	84-85	0.07311756916640633
2216	86-87	0.0806550983178056
2216	88-89	0.07765081633634452
2216	90-91	0.04725790101703353
2216	92-93	0.11638589296332924
2216	94-95	0.12377773230739209
2216	96-97	0.11503827605436001
2216	98-99	0.11330019571509808
2216	100	0.09046988676006151
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28959.0
3	4928.0
4	3141.0
5	2533.0
6	2262.0
7	2287.0
8	2628.0
9	3011.0
10	3469.0
11	3695.0
12	4164.0
13	4540.0
14	5013.0
15	5315.0
16	5173.0
17	5293.0
18	5199.0
19	5298.0
20	5332.0
21	5611.0
22	6626.0
23	8633.0
24	13477.0
25	18897.0
26	20667.0
27	17473.0
28	17406.0
29	22154.0
30	27492.0
31	33307.0
32	43798.0
33	60185.0
34	84294.0
35	129187.0
36	241511.0
37	519675.0
38	878808.0
39	407711.0
40	958.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.447008894524714	20.245585740396955	13.72691976069044	23.580485604387896
2	14.968512223249316	20.186126408782552	41.25004140157115	23.59531996639698
3	17.73984529495563	25.988875049889987	30.27646106662443	25.99481858852995
4	11.9309611086478	17.07205992576973	39.83361557985772	31.163363385724757
5	12.496643376526276	38.619711296532955	36.14961251213838	12.73403281480239
6	30.202272858005962	38.985188112141294	18.11052840734139	12.702010622511347
7	26.749945110966078	33.05071746734479	23.028543801449068	17.17079362024007
8	24.62051426276821	37.451328218879766	20.51742107614079	17.410736442211235
9	24.682639053387927	17.380047300523767	21.365210427171107	36.5721032189172
10-11	23.466336129028498	27.427034837867414	29.337314775246753	19.769314257857335
12-13	24.030675626288712	24.688209213591634	29.370090493586343	21.911024666533315
14-15	21.343777659629666	26.77405151675351	26.25031866149389	25.631852162122936
16-17	21.156141587403408	30.15708140999333	26.87127438551978	21.81550261708349
18-19	21.102686365918867	29.2750923602198	28.616637454435594	21.005583819425734
20-21	22.196491907547596	28.48483082323324	28.18516050007097	21.13351676914819
22-23	21.890520140063593	28.177909942251777	28.185410932044043	21.746158985640587
24-25	21.80169858118159	28.333200471084414	28.09889914852846	21.766201799205533
26-27	21.54770803503748	28.348552688573115	28.2127403412304	21.890998935159004
28-29	21.519585375180338	28.341594721835673	28.33195027299618	21.80686962998781
30-31	21.708606086675825	28.683634197840558	28.016787173525554	21.590972541958063
32-33	21.511466128800578	28.431039656343277	28.39913393933514	21.658360275521
34-35	21.639128962706273	28.230544057099106	28.394211808930965	21.736115171263656
36-37	21.933116250076605	28.501069061725858	27.91066952551856	21.655145162678973
38-39	21.725627008794	28.64724237948364	28.070168512637554	21.556962099084807
40-41	21.502593985202687	28.384390295987643	28.38392033707709	21.729095381732584
42-43	21.522249418995585	28.292842390322555	28.33372840818796	21.851179782493904
44-45	21.589443686250625	28.4586044972799	28.33494997800611	21.61700183846336
46-47	21.648412276950406	28.5963654136257	28.16634318503306	21.58887912439083
48-49	21.634956445046623	28.44823988814924	28.114815451134277	21.80198821566986
50-51	21.64116356765306	28.33831878613733	28.185606593918465	21.834911052291144
52-53	21.554105620909137	28.370703299760518	28.285399020260236	21.789792059070113
54-55	21.69988650405807	28.659191498368237	28.04915550974114	21.59176648783255
56-57	21.5911575084318	28.440167449900272	28.085057271196696	21.883617770471233
58-59	21.456298215252076	28.12301751327015	28.440835688116383	21.979848583361388
60-61	21.756870732724646	28.451240443361485	28.076164761870924	21.715724062042938
62-63	21.514767090993132	28.509978968199295	28.12911709857766	21.846136842229917
64-65	21.573810920136538	28.260984797221095	28.195008345739165	21.970195936903206
66-67	21.4316554159531	28.3264791478818	28.12715984847673	22.11470558768837
68-69	21.736866640100448	28.488094074480642	28.018060014586354	21.75697927083255
70-71	21.702004481135624	28.29438655057819	28.090949760153983	21.912659208132208
72-73	21.508251410071313	28.34435532575293	28.243323789064195	21.904069475111562
74-75	21.542174608112465	28.409922021922583	28.154042651574173	21.89386071839078
76-77	21.571053733179774	28.32592952380344	28.055742010962764	22.047274732054024
78-79	21.659252191588756	28.43009932896604	28.102730846377977	21.807917633067227
80-81	21.585995412054224	28.2683098660383	28.2123515139394	21.93334320796808
82-83	21.466967765122153	28.306212166893857	28.327621635480394	21.899198432503596
84-85	21.43581762150061	28.274099700471893	28.247596115439087	22.042486562588405
86-87	21.60100569897338	28.323591313741275	28.172409405044775	21.90299358224057
88-89	21.426904676361023	28.28604655560388	28.20341130419984	22.08363746383526
90-91	21.42363545999823	28.17437623351935	28.26031125726117	22.14167704922125
92-93	21.494218942096378	28.33339605546502	28.15101648621289	22.02136851622571
94-95	21.366423384163678	28.054836036265662	28.398062337020924	22.180678242549735
96-97	21.373487369535624	28.12447057933747	28.306874905460592	22.195167145666314
98-99	21.37667985841325	28.155898953149734	28.244121240035525	22.223299948401497
100	21.496261181099204	28.00302372771627	28.255971635346604	22.24474345583792
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	398.0
1	373.0
2	337.5
3	295.5
4	245.0
5	201.0
6	196.5
7	204.5
8	192.0
9	208.0
10	210.5
11	202.5
12	228.5
13	262.5
14	291.0
15	337.0
16	419.5
17	589.5
18	850.5
19	1282.0
20	1928.5
21	2823.0
22	4285.5
23	6618.0
24	9764.5
25	13633.0
26	18697.5
27	25141.0
28	32613.5
29	41072.5
30	49214.0
31	57169.5
32	64923.0
33	71767.0
34	78404.0
35	83813.5
36	88316.5
37	92976.5
38	96380.0
39	97510.5
40	98203.0
41	98422.0
42	96762.5
43	94782.0
44	92877.0
45	90149.5
46	87881.5
47	85305.5
48	82761.0
49	81124.5
50	78533.5
51	75383.0
52	70471.5
53	66092.5
54	63399.5
55	60117.5
56	55156.5
57	49548.5
58	45175.5
59	41089.0
60	36766.0
61	31983.0
62	27693.0
63	23495.5
64	19043.0
65	15389.0
66	12294.5
67	9267.5
68	6957.0
69	5309.0
70	3891.5
71	2815.5
72	2127.0
73	1657.0
74	1259.5
75	895.5
76	581.0
77	391.0
78	276.5
79	184.0
80	120.5
81	76.5
82	56.0
83	42.0
84	24.0
85	18.5
86	17.5
87	17.0
88	13.5
89	7.0
90	6.5
91	6.5
92	4.5
93	3.0
94	2.5
95	1.0
96	0.5
97	1.5
98	1.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3314148663025213
2	0.12052133182462378
3	0.06620026991365019
4	0.04086297183199191
5	0.04465980730120183
6	0.010789027521418288
7	0.007443301216866972
8	0.0172173331178034
9	0.019548063801872854
10-11	0.022724624169677195
12-13	0.01781881200401487
14-15	0.02060065185274293
16-17	0.02125851938453673
18-19	0.02069463292871347
20-21	0.025844795891899208
22-23	0.01733011040896805
24-25	0.02684099529718696
26-27	0.020976576156625102
28-29	0.020638244283131147
30-31	0.02178481340997177
32-33	0.026765810436410523
34-35	0.017048167181056423
36-37	0.011315321546853324
38-39	0.014980583509704486
40-41	0.01139050640762976
42-43	0.010394307002342009
44-45	0.010525880508700769
46-47	0.009304126521083714
48-49	0.005150162963185732
50-51	0.007687652014390382
52-53	0.008270334685407747
54-55	0.0038720203299863537
56-57	0.0034021149501336414
58-59	0.0037028543932393775
60-61	0.0042667408490626325
62-63	0.0034021149501336414
64-65	0.002631470127175192
66-67	0.002123972316934262
68-69	0.003383318734939533
70-71	0.002631470127175192
72-73	0.001860825304216743
74-75	0.0025374890512046494
76-77	0.003966001405956896
78-79	0.004153963557897981
80-81	0.003608873317268835
82-83	0.002706654987951626
84-85	0.0034772998109100753
86-87	0.0026126739119810834
88-89	0.5999751889959438
90-91	0.6109521786693032
92-93	0.6080011728838282
94-95	0.5936032720451411
96-97	0.5905770813988895
98-99	0.5884531090819553
100	0.5899004176519016
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2660110.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.80289409103113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.81401980973384	47.6133985672605
2	14.192996294001528	17.8272248617315
3	4.60694311753105	8.679880820811219
4	2.0093462167864615	5.047710305802168
5	1.0433040811309287	3.276125785600312
6	0.610038979960722	2.2987328069924327
7	0.3719122146757283	1.6350014400608417
8	0.2691796988160208	1.3524211292958566
9	0.18873188518465014	1.0667617737167003
>10	0.8513657211104432	9.072344842171054
>50	0.035405421523119825	1.4974070288620456
>100	0.006696625823284736	0.6111593726662596
>500	5.993372226565569E-5	0.02183126502920124
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.759243038821703E-5	0.0	0.0	0.0	0.0
6	3.759243038821703E-5	0.0	0.0	0.0	0.0
7	3.759243038821703E-5	0.0	0.0	0.0	0.0
8	3.759243038821703E-5	0.0	0.0	0.0	0.0
9	3.759243038821703E-5	0.0	0.0	7.518486077643406E-5	0.0
10-11	3.759243038821703E-5	0.0	0.0	9.398107597054258E-5	0.0
12-13	3.759243038821703E-5	0.0	0.0	1.1277729116465109E-4	0.0
14-15	5.638864558232554E-5	5.638864558232554E-5	0.0	1.1277729116465109E-4	0.0
16-17	7.518486077643406E-5	1.1277729116465109E-4	0.0	1.1277729116465109E-4	0.0
18-19	7.518486077643406E-5	1.3157350635875962E-4	0.0	1.1277729116465109E-4	0.0
20-21	7.518486077643406E-5	1.5036972155286812E-4	0.0	1.3157350635875962E-4	0.0
22-23	7.518486077643406E-5	1.5036972155286812E-4	0.0	1.8796215194108516E-4	0.0
24-25	7.518486077643406E-5	1.6916593674697663E-4	0.0	3.5712808868806174E-4	0.0
26-27	7.518486077643406E-5	1.8796215194108513E-4	0.0	4.5110916465860434E-4	0.0
28-29	7.518486077643406E-5	1.8796215194108513E-4	0.0	0.0011841615572288364	0.0
30-31	9.398107597054258E-5	1.8796215194108513E-4	0.0	0.004586276507362478	0.0
32-33	1.1277729116465109E-4	1.8796215194108513E-4	0.0	0.011747634496317822	0.0
34-35	1.1277729116465109E-4	1.8796215194108513E-4	0.0	0.021408889106089596	0.0
36-37	1.1277729116465109E-4	1.8796215194108513E-4	0.0	0.038720203299863545	0.0
38-39	1.1277729116465109E-4	1.8796215194108513E-4	0.0	0.06770396712917887	0.0
40-41	1.1277729116465109E-4	2.0675836713519364E-4	0.0	0.10097326802275094	0.0
42-43	1.503697215528681E-4	2.2555458232930217E-4	0.0	0.13238174361210625	0.0
44-45	2.631470127175192E-4	2.2555458232930217E-4	0.0	0.17254925548191616	0.0
46-47	2.631470127175192E-4	2.2555458232930217E-4	0.0	0.21969016318874032	0.0
48-49	2.631470127175192E-4	2.2555458232930217E-4	0.0	0.27489464721383705	0.0
50-51	2.631470127175192E-4	2.2555458232930217E-4	0.0	0.33720410058230676	0.0
52-53	2.819432279116277E-4	2.2555458232930217E-4	0.0	0.3998894782546586	3.759243038821703E-5
54-55	3.0073944310573625E-4	2.2555458232930217E-4	0.0	0.46479280931991535	3.759243038821703E-5
56-57	3.0073944310573625E-4	2.2555458232930217E-4	0.0	0.5444699655277414	3.759243038821703E-5
58-59	3.195356582998448E-4	2.2555458232930217E-4	0.0	0.6356316092191676	3.759243038821703E-5
60-61	3.3833187349395326E-4	2.2555458232930217E-4	0.0	0.7334095206589202	3.759243038821703E-5
62-63	3.3833187349395326E-4	2.4435079752341065E-4	0.0	0.8334429779219656	3.759243038821703E-5
64-65	4.135167342703873E-4	2.631470127175192E-4	0.0	0.9410513099082369	3.759243038821703E-5
66-67	4.5110916465860434E-4	2.631470127175192E-4	0.0	1.0620989357582955	3.759243038821703E-5
68-69	4.5110916465860434E-4	2.631470127175192E-4	0.0	1.2002323212197992	3.759243038821703E-5
70-71	4.5110916465860434E-4	2.819432279116277E-4	0.0	1.345865396543752	3.759243038821703E-5
72-73	4.5110916465860434E-4	3.0073944310573625E-4	0.0	1.505219708959404	3.759243038821703E-5
74-75	4.5110916465860434E-4	3.0073944310573625E-4	0.0	1.6639913387040386	3.759243038821703E-5
76-77	4.5110916465860434E-4	3.0073944310573625E-4	0.0	1.8400366902120588	3.759243038821703E-5
78-79	5.638864558232554E-4	3.0073944310573625E-4	0.0	2.0412501738649906	3.759243038821703E-5
80-81	5.638864558232554E-4	3.0073944310573625E-4	0.0	2.2629139396491125	3.759243038821703E-5
82-83	5.638864558232554E-4	3.0073944310573625E-4	0.0	2.4896714797508377	3.759243038821703E-5
84-85	5.82682671017364E-4	3.0073944310573625E-4	0.0	2.7190792861949316	3.759243038821703E-5
86-87	6.014788862114725E-4	3.0073944310573625E-4	0.0	2.959990376337821	3.759243038821703E-5
88	6.014788862114725E-4	3.0073944310573625E-4	0.0	3.15460638845762	3.759243038821703E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2175	0.0	35.519627	1
TCAACGC	3310	0.0	32.213547	4
GTATCAA	3565	0.0	32.10928	1
ATCAACG	3360	0.0	31.600327	3
CAACGCA	3475	0.0	30.819157	5
AACGCAG	3685	0.0	28.926668	6
GTACATG	7305	0.0	26.052235	1
TATCAAC	4260	0.0	25.60083	2
TACATGG	7370	0.0	24.684204	2
ACGCAGA	4330	0.0	24.075941	7
ACATGGG	7690	0.0	22.726835	3
CGCAGAG	4530	0.0	22.702002	8
CATGGGG	5015	0.0	18.826336	4
GTATAGG	955	0.0	18.25081	1
GCAGAGT	5490	0.0	18.133505	9
GTATTAG	1405	0.0	17.769833	1
GAGTACA	5425	0.0	17.540236	1
TAGACGG	270	5.4854536E-7	17.397104	5
CTAGACG	220	3.2133823E-5	17.080791	4
CTACACT	1395	0.0	16.835907	4
>>END_MODULE