FastQCFastQC Report
Wed 25 May 2016
SRR1033180_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033180_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2660110
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50020.18803733680186158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42100.1582641319343937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37300.049.32121
GTATCAA68800.041.032551
TCAACGC76350.036.4545364
ATCAACG77250.036.0298233
CAACGCA79250.035.239875
AACGCAG82600.034.081796
TATCAAC84850.033.02492
ACGCAGA96100.029.0984067
CGCAGAG98700.028.2350568
GTACATG71150.026.517661
TACATGG71300.025.3214632
GCAGAGT110600.024.8571829
ACATGGG73750.023.714823
AGAGTAC102150.020.564610-11
GAGTACT75550.020.55795712-13
CAGAGTA108150.020.44486410-11
CATGGGG50650.020.142774
GTGGTAT20600.019.870911
TGGTATC19100.019.692632
GTATAGA12150.018.9751641
Produced by FastQC (version 0.11.5)