##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033180_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2660110 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.762481250775345 34.0 31.0 34.0 31.0 34.0 2 32.84756156700287 34.0 31.0 34.0 31.0 34.0 3 32.93779204619358 34.0 31.0 34.0 31.0 34.0 4 36.369237362364714 37.0 37.0 37.0 35.0 37.0 5 36.306975651382835 37.0 37.0 37.0 35.0 37.0 6 36.39416715850096 37.0 37.0 37.0 35.0 37.0 7 36.36891519523629 37.0 37.0 37.0 35.0 37.0 8 36.361042212540085 37.0 37.0 37.0 35.0 37.0 9 38.17609008650018 39.0 39.0 39.0 37.0 39.0 10-11 38.158250034773 39.0 39.0 39.0 37.0 39.0 12-13 38.121636699234244 39.0 39.0 39.0 37.0 39.0 14-15 39.65751867403979 41.0 40.0 41.0 37.0 41.0 16-17 39.676967493825444 41.0 40.0 41.0 37.0 41.0 18-19 39.6944466582209 41.0 40.0 41.0 37.0 41.0 20-21 39.68919255218769 41.0 40.0 41.0 37.0 41.0 22-23 39.62260150896016 41.0 40.0 41.0 37.0 41.0 24-25 39.57678216314363 41.0 40.0 41.0 37.0 41.0 26-27 39.482582863114686 41.0 39.5 41.0 36.5 41.0 28-29 39.39357113051716 41.0 39.0 41.0 36.0 41.0 30-31 39.30092721729552 41.0 39.0 41.0 36.0 41.0 32-33 39.33860009548478 41.0 39.0 41.0 36.0 41.0 34-35 39.28435290269951 41.0 39.0 41.0 35.0 41.0 36-37 39.20377296427591 41.0 39.0 41.0 35.0 41.0 38-39 39.11877422362233 41.0 39.0 41.0 35.0 41.0 40-41 39.01082342459522 41.0 39.0 41.0 35.0 41.0 42-43 38.899819368371986 41.0 39.0 41.0 35.0 41.0 44-45 38.76301637902192 41.0 39.0 41.0 35.0 41.0 46-47 38.55933927544349 41.0 39.0 41.0 35.0 41.0 48-49 38.38010157474691 41.0 38.0 41.0 35.0 41.0 50-51 38.24893951753875 41.0 38.0 41.0 34.5 41.0 52-53 38.11984523196409 40.0 38.0 41.0 34.0 41.0 54-55 37.99022728383413 40.0 38.0 41.0 34.0 41.0 56-57 37.83801929243528 40.0 37.0 41.0 34.0 41.0 58-59 37.65669633962506 40.0 37.0 41.0 34.0 41.0 60-61 37.470736548488595 40.0 36.5 41.0 33.0 41.0 62-63 37.245004905812166 39.5 36.0 41.0 33.0 41.0 64-65 37.00144486506197 39.0 35.5 41.0 33.0 41.0 66-67 36.72681524448237 39.0 35.0 41.0 33.0 41.0 68-69 36.40842239606633 38.5 35.0 41.0 32.0 41.0 70-71 36.08148046509355 37.0 35.0 40.0 32.0 41.0 72-73 35.73336986064486 37.0 35.0 39.5 32.0 41.0 74-75 35.396761036197745 36.5 35.0 39.0 31.0 41.0 76-77 35.055108811289756 36.0 35.0 39.0 31.0 40.5 78-79 34.70881711658541 35.5 35.0 38.0 31.0 39.5 80-81 34.366440297581676 35.0 35.0 37.0 31.0 39.0 82-83 34.04097593708531 35.0 34.5 37.0 31.0 39.0 84-85 33.764637552582414 35.0 34.0 36.0 30.0 38.0 86-87 33.526184255538304 35.0 34.0 36.0 30.0 37.0 88-89 33.302279604978736 35.0 34.0 36.0 30.0 37.0 90-91 33.10189522237802 35.0 34.0 35.0 30.0 36.5 92-93 32.9376343459481 35.0 34.0 35.0 30.0 36.0 94-95 32.78743303848337 35.0 34.0 35.0 30.0 36.0 96-97 32.665427745469174 35.0 34.0 35.0 29.5 36.0 98-99 32.55938495024642 35.0 34.0 35.0 29.5 36.0 100 32.46655138321348 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0810146358513677 1101 2 0.07896643007671145 1101 3 0.10095482913170173 1101 4 0.11867277413769983 1101 5 0.04561532321530848 1101 6 0.05113487543690809 1101 7 0.06018488108476561 1101 8 0.08353878200880871 1101 9 0.07887310206939446 1101 10-11 0.10045346007449751 1101 12-13 0.1281627923413282 1101 14-15 0.17818368446655342 1101 16-17 0.13463646942404495 1101 18-19 0.11765671314908133 1101 20-21 0.16158317486838314 1101 22-23 0.1970291744887902 1101 24-25 0.12566683282241797 1101 26-27 0.12836895800531067 1101 28-29 0.12782116647098718 1101 30-31 0.14273819323757664 1101 32-33 0.10544600139660076 1101 34-35 0.09430953562623756 1101 36-37 0.1530479057814702 1101 38-39 0.11724196453494073 1101 40-41 0.194660223175525 1101 42-43 0.1401012800666166 1101 44-45 0.08085267524208462 1101 46-47 0.12100376063214924 1101 48-49 0.013033880641053486 1101 50-51 0.0617222154149033 1101 52-53 0.013726693550715652 1101 54-55 0.0457289774521783 1101 56-57 0.00676120241224254 1101 58-59 -0.017460813342026427 1101 60-61 0.0015152828092581672 1101 62-63 0.007748906119957155 1101 64-65 0.02195512298609259 1101 66-67 -0.006952388080023297 1101 68-69 -0.010573018332962647 1101 70-71 -0.035458273431949294 1101 72-73 0.0734628183580881 1101 74-75 0.0049979059903009215 1101 76-77 0.020777025338595934 1101 78-79 -0.04147918164806441 1101 80-81 0.008665996049394664 1101 82-83 6.634281919417617E-4 1101 84-85 0.0616837015695495 1101 86-87 0.035421822500232736 1101 88-89 0.008873433050027302 1101 90-91 0.07129517409946118 1101 92-93 0.10320576520192759 1101 94-95 -0.036274238534723224 1101 96-97 0.05142020966138716 1101 98-99 0.06433037569060218 1101 100 0.021082608718117513 1102 1 0.11682857268474578 1102 2 0.10796755330130026 1102 3 0.0973367911894698 1102 4 0.03893344628795603 1102 5 0.01595692642715818 1102 6 0.041369805605661725 1102 7 0.07600302316683383 1102 8 0.10574765916745577 1102 9 0.1288339571524162 1102 10-11 0.1017288696830363 1102 12-13 0.1600293146110232 1102 14-15 0.18360175232790255 1102 16-17 0.15566807670649752 1102 18-19 0.15971108662990474 1102 20-21 0.18908120282574004 1102 22-23 0.21462481837200187 1102 24-25 0.22436939818688728 1102 26-27 0.21194198706132994 1102 28-29 0.173262488972874 1102 30-31 0.16706193908726163 1102 32-33 0.20150164237075785 1102 34-35 0.20780460333710238 1102 36-37 0.14343535389836148 1102 38-39 0.20530914967137903 1102 40-41 0.20655277596419808 1102 42-43 0.15321005529014542 1102 44-45 0.14194700761149903 1102 46-47 0.12380135337141951 1102 48-49 0.1638496056669112 1102 50-51 0.16198669330042748 1102 52-53 0.21060561331376704 1102 54-55 0.15127827807433647 1102 56-57 0.16450082797346965 1102 58-59 0.15270367965102594 1102 60-61 0.23781604154443414 1102 62-63 0.24990941699996227 1102 64-65 0.17800132952476133 1102 66-67 0.10419498339539501 1102 68-69 0.22289652194990595 1102 70-71 0.17122947767887808 1102 72-73 0.16367901578637856 1102 74-75 0.17454100764383185 1102 76-77 0.1665481165769549 1102 78-79 0.15220954945439757 1102 80-81 0.12708177440290314 1102 82-83 0.11253665636841248 1102 84-85 0.1576404259797144 1102 86-87 0.14052425996121087 1102 88-89 0.14881832387262506 1102 90-91 0.17247442674597835 1102 92-93 0.1398360411280919 1102 94-95 0.14886668094689526 1102 96-97 0.17633133642092957 1102 98-99 0.11734477104701568 1102 100 0.16572481559474994 1103 1 0.12457317152405523 1103 2 0.1224783713660571 1103 3 0.09995369903759155 1103 4 0.10118170424699002 1103 5 0.04752641420764547 1103 6 0.07681645894197686 1103 7 0.08001028630443585 1103 8 0.09972953681762675 1103 9 0.1344798200850974 1103 10-11 0.07276738042782682 1103 12-13 0.07466075560418517 1103 14-15 0.14324792268589448 1103 16-17 0.1414841127147568 1103 18-19 0.10982034569913424 1103 20-21 0.12621154643363042 1103 22-23 0.14305304350750703 1103 24-25 0.170314854811501 1103 26-27 0.12649306524682657 1103 28-29 0.09604134469522307 1103 30-31 0.1097135224492547 1103 32-33 0.11692322222484108 1103 34-35 0.12348122597525446 1103 36-37 0.15121254869394107 1103 38-39 0.12732395045391343 1103 40-41 0.1492982829103937 1103 42-43 0.040944465299048716 1103 44-45 0.11323512251758672 1103 46-47 0.16874237508541512 1103 48-49 0.12750228777383654 1103 50-51 0.13705372074815614 1103 52-53 0.10393500650801712 1103 54-55 0.06961667719854603 1103 56-57 0.0776886008617339 1103 58-59 0.19584834572889065 1103 60-61 0.19705034356804418 1103 62-63 0.12373877133668998 1103 64-65 0.1478349706412061 1103 66-67 0.14455747189736456 1103 68-69 0.17423920341705923 1103 70-71 0.20413293081791295 1103 72-73 0.26864661805359447 1103 74-75 0.2901015085295384 1103 76-77 0.20606124963443762 1103 78-79 0.10315288573174541 1103 80-81 0.1714071941107278 1103 82-83 0.11483712224369924 1103 84-85 0.161391859164155 1103 86-87 0.21190418025103952 1103 88-89 0.14345763083178298 1103 90-91 0.18111561117496677 1103 92-93 0.1669441407688126 1103 94-95 0.22671405401337097 1103 96-97 0.29924635808310285 1103 98-99 0.3134090186428864 1103 100 0.28292227037653106 1104 1 0.031336776259024646 1104 2 0.09932889597425287 1104 3 0.06671685955534201 1104 4 0.050855389682794794 1104 5 -0.018737632619426847 1104 6 0.04311435676626019 1104 7 0.06794855491847329 1104 8 0.07658348902780432 1104 9 0.08821158061527257 1104 10-11 0.09972084277883653 1104 12-13 0.10711150884714016 1104 14-15 0.10878979434029645 1104 16-17 0.10226477100354003 1104 18-19 0.06740956377948493 1104 20-21 0.08704732056234121 1104 22-23 0.07701158515335749 1104 24-25 0.07304266386500302 1104 26-27 0.017607953118314867 1104 28-29 0.09197600308464615 1104 30-31 0.07147512615018314 1104 32-33 0.09398965148208305 1104 34-35 0.04763624325105553 1104 36-37 0.09560796700466057 1104 38-39 0.037167255572597924 1104 40-41 0.04023531251486645 1104 42-43 0.0065326095802547 1104 44-45 -0.0040579315566802165 1104 46-47 0.02513801989206854 1104 48-49 -0.018363770589459705 1104 50-51 0.050414876612322246 1104 52-53 0.06538726912979342 1104 54-55 0.050443185833564996 1104 56-57 0.05238117581207291 1104 58-59 0.10474789491696157 1104 60-61 0.07100028056186858 1104 62-63 0.07234307922912109 1104 64-65 0.08714938032108677 1104 66-67 0.0740260263226773 1104 68-69 0.1310102529035504 1104 70-71 0.17719677785692767 1104 72-73 0.157720540322984 1104 74-75 0.12992219558824303 1104 76-77 0.0705577575249805 1104 78-79 0.05069597377353574 1104 80-81 0.13135922961958357 1104 82-83 0.08089200308722866 1104 84-85 0.12885185116550701 1104 86-87 0.16334472595818283 1104 88-89 0.0892451011367541 1104 90-91 0.13240698764371217 1104 92-93 0.0730111882283424 1104 94-95 0.016893741248807714 1104 96-97 0.08313728685218535 1104 98-99 0.13830445278912862 1104 100 0.16522472499200092 1105 1 0.02602945090536224 1105 2 0.055788676174984175 1105 3 0.06060260210639967 1105 4 0.014448547072483109 1105 5 0.014222181530008982 1105 6 0.016503707989031113 1105 7 0.008074430530015775 1105 8 -0.1017768879128127 1105 9 0.015167399815325666 1105 10-11 0.012158897915092837 1105 12-13 -0.03702551010611188 1105 14-15 -0.007359397677213053 1105 16-17 0.06291143754683759 1105 18-19 0.03246156061866401 1105 20-21 0.05144280013572455 1105 22-23 0.07994704513988182 1105 24-25 -0.014916984656899501 1105 26-27 0.06455779523871996 1105 28-29 0.09255945325179482 1105 30-31 0.08179029724409048 1105 32-33 0.0011624960998091183 1105 34-35 0.020597037335981838 1105 36-37 -0.024606113338066393 1105 38-39 0.04121643727049218 1105 40-41 0.03809064993306066 1105 42-43 0.12676904799417343 1105 44-45 0.09670521954970468 1105 46-47 0.10804039783593566 1105 48-49 0.020290700270734874 1105 50-51 0.011566526041981717 1105 52-53 0.009017105610297449 1105 54-55 0.04188791434368966 1105 56-57 0.01959298208541327 1105 58-59 0.030991120569566988 1105 60-61 -0.013254974720879886 1105 62-63 -0.0121547561868951 1105 64-65 -0.05364561737165019 1105 66-67 -0.025218883330630604 1105 68-69 -0.04994807263958023 1105 70-71 0.00868221715483486 1105 72-73 -0.027577984050900284 1105 74-75 -0.13943750679059264 1105 76-77 -0.14635221478722826 1105 78-79 -0.09497492740425173 1105 80-81 -0.016312208547049067 1105 82-83 -0.03680457161016193 1105 84-85 -0.05021963756526304 1105 86-87 -0.015170675723496174 1105 88-89 -0.01651213183099287 1105 90-91 -0.08002926304143898 1105 92-93 0.004171345579116803 1105 94-95 -0.06123217558851479 1105 96-97 -0.033937048564844474 1105 98-99 -0.06446906100336491 1105 100 -0.08663595057210216 1106 1 0.05482946627841301 1106 2 0.03705189558031918 1106 3 0.01418035240872939 1106 4 0.04467319016218596 1106 5 -0.005824802360166359 1106 6 0.006101567291544541 1106 7 0.010718857514788738 1106 8 0.04937864588366381 1106 9 0.07984165488832673 1106 10-11 0.0481235416424326 1106 12-13 0.0728210607206421 1106 14-15 0.06109937961487333 1106 16-17 0.0725785541955517 1106 18-19 0.1256325190018046 1106 20-21 0.08592429373253907 1106 22-23 0.07631778201131567 1106 24-25 0.11351285461173433 1106 26-27 0.02600222449451195 1106 28-29 0.028893617981914588 1106 30-31 0.1087724055446202 1106 32-33 0.06943500615670928 1106 34-35 0.09090254617308346 1106 36-37 0.12370431400221094 1106 38-39 0.09635197561773623 1106 40-41 0.06785017085285716 1106 42-43 0.07615698652467984 1106 44-45 0.14179455576140754 1106 46-47 0.18118533558161687 1106 48-49 0.11081111738705829 1106 50-51 0.054319114676140146 1106 52-53 0.09958119616980099 1106 54-55 0.07798947431540881 1106 56-57 0.1122178040615438 1106 58-59 0.057713153515940974 1106 60-61 0.12779976513606783 1106 62-63 0.1298701892103864 1106 64-65 0.10102257146008498 1106 66-67 0.15500045343572566 1106 68-69 0.18102046020786844 1106 70-71 0.11156374726264318 1106 72-73 0.18082513580518622 1106 74-75 0.1725352182706672 1106 76-77 0.0894173396694029 1106 78-79 0.10072992047896889 1106 80-81 0.10244789366628027 1106 82-83 0.11057037057711483 1106 84-85 0.1633081241139962 1106 86-87 0.08647533267664897 1106 88-89 0.06464884804385207 1106 90-91 0.077694974977085 1106 92-93 0.04711893613709606 1106 94-95 -0.01027160004241523 1106 96-97 0.0629513933020931 1106 98-99 0.06300629516448453 1106 100 0.1668320483675032 1107 1 -0.04763493651439887 1107 2 -0.06386783620089886 1107 3 -0.016101347962710122 1107 4 -0.010006053853814478 1107 5 -0.10014075663259803 1107 6 -0.13777111010345067 1107 7 -0.1100525340086449 1107 8 -0.05897648413905898 1107 9 -0.07538123495996274 1107 10-11 -0.08538871834357309 1107 12-13 -0.04458270687813126 1107 14-15 -0.025130998014844863 1107 16-17 -0.027723851699626323 1107 18-19 -0.04179444729506798 1107 20-21 -0.10589696109730795 1107 22-23 -0.07506859714300163 1107 24-25 -0.08222508310507948 1107 26-27 -0.08449865700779213 1107 28-29 -0.07802817790173577 1107 30-31 -0.04887976203117006 1107 32-33 -0.0317262971007537 1107 34-35 -0.05662254727121763 1107 36-37 -0.09514621205698148 1107 38-39 -0.12719046226674635 1107 40-41 -0.049754931426058135 1107 42-43 -0.09837136739613328 1107 44-45 -0.10142105324616324 1107 46-47 -0.16193064289920045 1107 48-49 -0.186828571055365 1107 50-51 -0.21996641394764538 1107 52-53 -0.20216379079257507 1107 54-55 -0.1688783096220945 1107 56-57 -0.1889639883150238 1107 58-59 -0.16065709288334773 1107 60-61 -0.1917487532968707 1107 62-63 -0.126110611176955 1107 64-65 -0.1499890599578393 1107 66-67 -0.11877709693968797 1107 68-69 -0.16567406137301077 1107 70-71 -0.17721603979639156 1107 72-73 -0.17772290478942665 1107 74-75 -0.12100697789163917 1107 76-77 -0.15459863497159176 1107 78-79 -0.10582110613802342 1107 80-81 -0.12906815105003488 1107 82-83 -0.15407071448901632 1107 84-85 -0.14492538529475496 1107 86-87 -0.07985658996767597 1107 88-89 -0.07437669692663462 1107 90-91 -0.13862575671413424 1107 92-93 -0.1780761641001476 1107 94-95 -0.19393973466394243 1107 96-97 -0.09064397088852871 1107 98-99 -0.14323869068271478 1107 100 -0.11632751726317281 1108 1 0.04437263216207299 1108 2 0.04363897150921758 1108 3 0.003887242687376613 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-0.09703496565592218 2214 16-17 -0.12152701233610941 2214 18-19 -0.150160195890372 2214 20-21 -0.20175652719073867 2214 22-23 -0.1588678041234246 2214 24-25 -0.15476171163260943 2214 26-27 -0.1484766043808392 2214 28-29 -0.11327327827747524 2214 30-31 -0.09617283075858296 2214 32-33 -0.14718701732989103 2214 34-35 -0.1299317953504726 2214 36-37 -0.11132569947422866 2214 38-39 -0.13579873357984695 2214 40-41 -0.08816048934136944 2214 42-43 -0.21056285167323097 2214 44-45 -0.22052788378756105 2214 46-47 -0.2396620913401577 2214 48-49 -0.21980930724444647 2214 50-51 -0.21424323787178423 2214 52-53 -0.2797832885692557 2214 54-55 -0.23993531314341254 2214 56-57 -0.26288949375641124 2214 58-59 -0.2674072976833841 2214 60-61 -0.3257325771986146 2214 62-63 -0.3217470947623866 2214 64-65 -0.23531352356666702 2214 66-67 -0.34694117274424485 2214 68-69 -0.23827831037927183 2214 70-71 -0.09735885728416349 2214 72-73 -0.2151773294272772 2214 74-75 -0.20436909195399977 2214 76-77 -0.1407656378156048 2214 78-79 -0.34577570985896955 2214 80-81 -0.16009895556724985 2214 82-83 -0.19347054288375176 2214 84-85 -0.23061571527889413 2214 86-87 -0.24199978823411072 2214 88-89 -0.39265558059115335 2214 90-91 -0.2645414055082256 2214 92-93 -0.24953647741342877 2214 94-95 -0.24922636279832489 2214 96-97 -0.5014339326257513 2214 98-99 -0.5395351219428122 2214 100 -0.6449273737038652 2215 1 -0.02850395185394916 2215 2 -0.07602134609258115 2215 3 -0.07447108967198801 2215 4 -0.060735897234032166 2215 5 -0.07557255880542613 2215 6 -0.06884370921586935 2215 7 -0.09078933163475256 2215 8 -0.06617028118454726 2215 9 -0.1174425945521449 2215 10-11 -0.07241247892400082 2215 12-13 -0.06513623345189501 2215 14-15 -0.15494767302877932 2215 16-17 -0.13790502147288208 2215 18-19 -0.12100452548882146 2215 20-21 -0.11188995857806816 2215 22-23 -0.12252490402137539 2215 24-25 -0.10200818517935062 2215 26-27 -0.10265787351424649 2215 28-29 -0.10619348785055394 2215 30-31 -0.12213430264964842 2215 32-33 -0.17622252918961578 2215 34-35 -0.18041714865537273 2215 36-37 -0.11426560897759686 2215 38-39 -0.20163525600471388 2215 40-41 -0.20635797534909983 2215 42-43 -0.1599971838979215 2215 44-45 -0.11080236993273473 2215 46-47 -0.21776042679746155 2215 48-49 -0.19874162199565149 2215 50-51 -0.1987628525492653 2215 52-53 -0.1679082424679379 2215 54-55 -0.10421872137347776 2215 56-57 -0.25514512156286884 2215 58-59 -0.2684265976794151 2215 60-61 -0.17100450961343228 2215 62-63 -0.2559633043038545 2215 64-65 -0.21527120887375162 2215 66-67 -0.1854308444334336 2215 68-69 -0.1771709949740341 2215 70-71 -0.16718383388962366 2215 72-73 -0.07371541045874608 2215 74-75 -0.12772463630953013 2215 76-77 -0.12304124113651227 2215 78-79 -0.09707262956536056 2215 80-81 -0.1098418745843901 2215 82-83 -0.04997556451348828 2215 84-85 -0.11348469655367666 2215 86-87 -0.16316501678034 2215 88-89 -0.16051349345269728 2215 90-91 -0.03602510477827536 2215 92-93 -0.07422547805521873 2215 94-95 0.004040164505632049 2215 96-97 0.05518044580359316 2215 98-99 -0.026175578244632902 2215 100 -0.04024199034487452 2216 1 -0.0376202656952529 2216 2 -0.07770960325736809 2216 3 -0.12428986141508602 2216 4 -0.04255439138010075 2216 5 -0.03358096962886492 2216 6 -0.0425730901355621 2216 7 -0.03244686531343177 2216 8 -0.059255000269871516 2216 9 -0.06481427521204353 2216 10-11 -0.1479577383478059 2216 12-13 -0.08081192225097311 2216 14-15 -0.17396533685739968 2216 16-17 -0.12072423915773811 2216 18-19 -0.11570995770990322 2216 20-21 -0.11621314289997287 2216 22-23 -0.13622108781809317 2216 24-25 -0.20572221351760334 2216 26-27 -0.20772314889759969 2216 28-29 -0.27054899894171314 2216 30-31 -0.17845990747103713 2216 32-33 -0.19071616312480444 2216 34-35 -0.19219140821913072 2216 36-37 -0.19728432089532788 2216 38-39 -0.22725068197070186 2216 40-41 -0.18997166564655288 2216 42-43 -0.22002238750915382 2216 44-45 -0.1833475888163889 2216 46-47 -0.2083031404366551 2216 48-49 -0.14926134913518752 2216 50-51 -0.09436195480230936 2216 52-53 -0.019147294048075025 2216 54-55 -0.012194528288709705 2216 56-57 0.007273440241746698 2216 58-59 -0.06839581464279121 2216 60-61 -7.95113961849836E-4 2216 62-63 0.11034282978879162 2216 64-65 0.09020950007973028 2216 66-67 0.11700227518225148 2216 68-69 0.01763342853000438 2216 70-71 0.025869163093226177 2216 72-73 0.060180011045538606 2216 74-75 0.07112040431194089 2216 76-77 0.0727906578309927 2216 78-79 0.06317494941981039 2216 80-81 0.09705856450905515 2216 82-83 0.08301975092216196 2216 84-85 0.06068412137543078 2216 86-87 0.027451081419386014 2216 88-89 0.028395497553105997 2216 90-91 0.02848155010654807 2216 92-93 0.015350272607683735 2216 94-95 -0.013241052047305857 2216 96-97 0.03437344925912811 2216 98-99 0.055504919029672806 2216 100 0.06375785757226282 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12.0 3 7.0 4 19.0 5 37.0 6 48.0 7 128.0 8 293.0 9 497.0 10 969.0 11 1591.0 12 2450.0 13 3777.0 14 4449.0 15 6177.0 16 7580.0 17 11356.0 18 13329.0 19 7128.0 20 4800.0 21 4673.0 22 4745.0 23 4906.0 24 5797.0 25 6770.0 26 8788.0 27 11271.0 28 14522.0 29 18525.0 30 24223.0 31 30926.0 32 40224.0 33 53320.0 34 74110.0 35 116085.0 36 217694.0 37 501603.0 38 950502.0 39 504771.0 40 2008.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.2909340475343 20.29471325227584 13.900241637324276 23.514111062865588 2 15.237051586891347 20.473558076370026 40.76542954615942 23.523960790579213 3 17.826706414396398 26.004638905909903 30.683505569318566 25.485149110375133 4 12.01034845979822 17.190781119248634 39.5618069355131 31.237063485440054 5 12.217049587469948 38.19525482319254 36.35879189749534 13.228903691842175 6 30.006210278838513 39.08893104259755 17.800831996920426 13.104026681643512 7 26.507849444942984 33.08748092573037 23.060101195602854 17.344568433723797 8 24.839320252396053 36.78110450527329 20.368030585258854 18.011544657071806 9 24.988609485028427 16.46294060241163 21.56081632775812 36.98763358480182 10-11 23.633752315705898 26.9144609818593 29.289694808006928 20.16209189442787 12-13 24.522839281082362 24.2410088304619 29.57073203739695 21.66541985105879 14-15 21.57131678750456 26.20308622981824 26.982754261906432 25.242842720770764 16-17 20.781245888327927 29.745142118183082 27.53438767569762 21.93922431779137 18-19 20.613358670655227 28.366936298627028 30.156732387694856 20.862972643022886 20-21 21.738969441113337 27.509651856502177 29.93676953208702 20.81460917029747 22-23 21.371146154866935 27.284544996941847 29.990718404512904 21.353590443678307 24-25 21.215055701675325 27.4726576002445 29.91556547411506 21.396721223965113 26-27 21.00169206137134 27.535469944576622 30.010499727638646 21.45233826641339 28-29 21.052885843566056 27.469186219953873 30.090554513636754 21.387373422843318 30-31 21.21188588998076 27.843508816529173 29.75415480041953 21.190450493070536 32-33 21.06308194872036 27.562087847524147 30.10484359944537 21.269986604310123 34-35 21.16689482405183 27.464452289724345 30.057286587297604 21.31136629892622 36-37 21.49401401320852 27.69861994318685 29.556262184284954 21.251103859319677 38-39 21.285844951460966 27.888563739033245 29.62318442765113 21.202406881854664 40-41 21.20872147625454 27.650967971804587 29.75244401952019 21.387866532420684 42-43 21.231714172374183 27.621305468498836 29.639218576389215 21.507761782737763 44-45 21.328970161302756 27.83654122735082 29.468782677259505 21.36570593408692 46-47 21.433759985622434 27.983730489803545 29.17546308811636 21.40704643645766 48-49 21.494809537675426 27.98815563623321 28.844977151739553 21.672057674351805 50-51 21.55682398133014 27.948937977989797 28.76408063829407 21.730157402385988 52-53 21.510024126325327 28.03674494799259 28.819050680321016 21.634180245361062 54-55 21.568293895565496 28.344403226700827 28.613238571161386 21.474064306572295 56-57 21.450183596690657 28.10705092117889 28.670632142155622 21.77213333997483 58-59 21.30840973934396 27.758130264298792 29.044352671812256 21.88910732454499 60-61 21.58127297148857 28.02742462569889 28.725615025952116 21.665687376860415 62-63 21.363821509174855 28.054906084220733 28.80041292154209 21.780859485062326 64-65 21.386908388114954 27.8655346750593 28.876124211311033 21.871432725514715 66-67 21.268880448916637 27.949624317646037 28.75598710270656 22.025508130730767 68-69 21.62948919938092 28.07309482161996 28.665238889521703 21.632177089477416 70-71 21.551221336811903 27.93743462410682 28.623443313637942 21.887900725443334 72-73 21.473538984076683 28.000097740962016 28.686427257698742 21.839936017262556 74-75 21.499370603883815 28.040165565857837 28.6089998099688 21.851464020289544 76-77 21.50920755551111 27.96451484118254 28.513103652572198 22.013173950734146 78-79 21.5601625122293 28.125348317690168 28.55187243372683 21.7626167363537 80-81 21.477385548088392 27.992651379219737 28.598138183994458 21.931824888697417 82-83 21.412032011632665 27.910802450513522 28.752294795431215 21.924870742422595 84-85 21.310897347338063 27.94521186503598 28.62274240287448 22.121148384751486 86-87 21.464246712065876 28.00118792303311 28.541523736844976 21.993041628056034 88-89 21.234370239270227 27.979750457640932 28.582037694312962 22.20384160877588 90-91 21.162752668122746 27.87984331475014 28.609437203724656 22.347966813402454 92-93 21.26135761303104 27.957960385096857 28.505663299637984 22.275018702234117 94-95 21.105179860983192 27.716429019852562 28.73104871603054 22.447342403133703 96-97 21.068226501911575 27.77599422580269 28.661201980369235 22.4945772919165 98-99 21.035727845840963 27.811105555785286 28.588986921593467 22.564179676780284 100 21.10653318847717 27.668141543018898 28.55588678663664 22.669438481867292 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 63.0 1 54.5 2 140.5 3 259.5 4 191.0 5 129.0 6 292.5 7 742.5 8 1319.5 9 1594.5 10 1347.5 11 1023.5 12 1033.5 13 1156.0 14 1351.5 15 1567.0 16 1753.5 17 1982.5 18 2280.0 19 2732.0 20 3330.5 21 4210.5 22 5547.0 23 7774.0 24 10940.0 25 14627.5 26 19308.0 27 25203.5 28 32417.0 29 40530.5 30 48431.5 31 56491.5 32 64268.5 33 71014.0 34 77489.0 35 82914.0 36 87148.5 37 91833.5 38 95184.0 39 96244.5 40 97102.5 41 97530.0 42 96273.0 43 94622.0 44 92738.5 45 90248.0 46 88260.5 47 85486.5 48 82453.5 49 80399.5 50 78095.0 51 75143.5 52 70124.0 53 65541.5 54 62670.5 55 59320.5 56 54836.5 57 49454.5 58 44998.5 59 41062.0 60 36727.0 61 31769.5 62 27343.0 63 23234.5 64 18763.0 65 15083.5 66 11953.0 67 8796.0 68 6413.5 69 4997.0 70 3702.0 71 2588.5 72 1952.5 73 1462.5 74 1077.0 75 767.5 76 470.5 77 288.5 78 202.0 79 131.5 80 66.5 81 29.5 82 20.5 83 8.0 84 2.5 85 2.5 86 2.0 87 2.5 88 1.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0905225723748266 2 0.001315735063587596 3 0.0 4 1.5036972155286812E-4 5 0.03875779573025176 6 1.5036972155286812E-4 7 0.004247944633868524 8 1.8796215194108513E-4 9 7.518486077643406E-5 10-11 5.262940254350385E-4 12-13 0.0 14-15 1.3157350635875962E-4 16-17 0.0 18-19 9.398107597054257E-5 20-21 0.0 22-23 2.6314701271751924E-4 24-25 5.638864558232555E-5 26-27 0.0015412896459168982 28-29 0.008289130900601854 30-31 0.03597595588152369 32-33 0.02528090943607595 34-35 0.02862663574062727 36-37 0.025957573183063857 38-39 0.02452906082831161 40-41 0.030543849690426336 42-43 0.022743420384871302 44-45 0.021690832334001226 46-47 0.0154504888895572 48-49 0.01050708429350666 50-51 0.007800429305555034 52-53 0.005732845634203097 54-55 0.004417110570615501 56-57 0.0025374890512046494 58-59 0.0012593464180052705 60-61 7.142561773761236E-4 62-63 0.0015224934307227897 64-65 0.002951005785475037 66-67 6.390713165996895E-4 68-69 0.0011653653420347278 70-71 4.699053798527129E-4 72-73 6.57867531793798E-4 74-75 7.706448229584491E-4 76-77 4.511091646586044E-4 78-79 9.398107597054257E-5 80-81 7.142561773761236E-4 82-83 5.45090240629147E-4 84-85 3.195356582998447E-4 86-87 1.8796215194108513E-4 88-89 1.8796215194108515E-5 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2660110.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.72485396318208 #Duplication Level Percentage of deduplicated Percentage of total 1 74.41454283156192 45.18812246183555 2 14.919164271607308 18.11928143292155 3 4.976865865997655 9.0665835872116 4 2.079758076934185 5.05172022002307 5 1.096743503523734 3.329979454327371 6 0.6440720920796097 2.346671023997728 7 0.41113571916624597 1.7476309553792684 8 0.28086768520365296 1.3644519333575058 9 0.1996852469496927 1.0913271713659836 >10 0.9273215983625034 9.574229466458638 >50 0.04007428032052433 1.657978802627214 >100 0.009335343181492211 0.922997104639702 >500 1.8577933350899856E-4 0.06253668758933115 >1k 1.857793334510649E-4 0.2883907738929961 >5k 6.19264444836883E-5 0.18809892437250833 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5002 0.18803733680186158 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4210 0.1582641319343937 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.759243038821703E-5 0.0 10-11 0.0 0.0 0.0 3.759243038821703E-5 0.0 12-13 0.0 1.8796215194108515E-5 0.0 3.759243038821703E-5 0.0 14-15 5.638864558232554E-5 5.638864558232554E-5 0.0 3.759243038821703E-5 0.0 16-17 1.1277729116465109E-4 7.518486077643406E-5 0.0 3.759243038821703E-5 0.0 18-19 1.1277729116465109E-4 7.518486077643406E-5 0.0 3.759243038821703E-5 0.0 20-21 1.1277729116465109E-4 7.518486077643406E-5 0.0 5.638864558232554E-5 0.0 22-23 1.3157350635875962E-4 7.518486077643406E-5 0.0 1.1277729116465109E-4 0.0 24-25 1.5036972155286812E-4 7.518486077643406E-5 0.0 2.819432279116277E-4 0.0 26-27 1.6916593674697663E-4 7.518486077643406E-5 0.0 3.7592430388217027E-4 0.0 28-29 1.8796215194108513E-4 9.398107597054258E-5 0.0 0.001090180481258294 0.0 30-31 2.0675836713519364E-4 1.1277729116465109E-4 0.0 0.004454703001003718 0.0 32-33 2.4435079752341065E-4 1.1277729116465109E-4 0.0 0.011578468559570846 0.0 34-35 2.631470127175192E-4 1.1277729116465109E-4 0.0 0.021314908030119054 0.0 36-37 2.631470127175192E-4 1.1277729116465109E-4 0.0 0.03860742600869889 0.0 38-39 2.631470127175192E-4 1.1277729116465109E-4 0.0 0.06759118983801421 0.0 40-41 2.631470127175192E-4 1.1277729116465109E-4 0.0 0.10093567559236272 0.0 42-43 2.631470127175192E-4 1.1277729116465109E-4 0.0 0.13260729819443556 0.0 44-45 2.631470127175192E-4 1.1277729116465109E-4 0.0 0.17309434572254528 0.0 46-47 2.631470127175192E-4 1.3157350635875962E-4 0.0 0.2205547890876693 0.0 48-49 2.631470127175192E-4 2.0675836713519364E-4 0.0 0.27606001255587176 0.0 50-51 3.195356582998448E-4 2.4435079752341065E-4 0.0 0.33904612967132935 0.0 52-53 3.3833187349395326E-4 2.631470127175192E-4 0.0 0.4020134505715929 0.0 54-55 3.3833187349395326E-4 2.631470127175192E-4 0.0 0.4671047437887907 0.0 56-57 3.3833187349395326E-4 2.631470127175192E-4 0.0 0.5474021750980222 0.0 58-59 3.759243038821703E-4 3.0073944310573625E-4 0.0 0.6389397430933308 0.0 60-61 4.1351673427038733E-4 3.195356582998448E-4 0.0 0.7369056166850243 0.0 62-63 4.1351673427038733E-4 3.3833187349395326E-4 0.0 0.8379164771381635 0.0 64-65 4.1351673427038733E-4 3.3833187349395326E-4 0.0 0.9460511031498697 0.0 66-67 4.1351673427038733E-4 3.3833187349395326E-4 0.0 1.0685836300002631 0.0 68-69 4.1351673427038733E-4 3.3833187349395326E-4 0.0 1.2079763618797719 0.0 70-71 4.1351673427038733E-4 3.3833187349395326E-4 0.0 1.3555266511535238 0.0 72-73 4.1351673427038733E-4 3.3833187349395326E-4 0.0 1.5159147554048515 0.0 74-75 4.1351673427038733E-4 3.5712808868806174E-4 0.0 1.6752878640356978 0.0 76-77 4.1351673427038733E-4 4.1351673427038733E-4 0.0 1.8525361733161412 0.0 78-79 4.1351673427038733E-4 4.1351673427038733E-4 0.0 2.0556668709188717 0.0 80-81 4.5110916465860434E-4 4.1351673427038733E-4 0.0 2.2790598885008517 0.0 82-83 4.5110916465860434E-4 4.1351673427038733E-4 0.0 2.508956396539993 0.0 84-85 4.5110916465860434E-4 4.1351673427038733E-4 0.0 2.7412024314783974 0.0 86-87 4.5110916465860434E-4 4.1351673427038733E-4 0.0 2.9859667457360786 0.0 88 4.5110916465860434E-4 4.1351673427038733E-4 0.0 3.1834397825653826 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3730 0.0 49.3212 1 GTATCAA 6880 0.0 41.03255 1 TCAACGC 7635 0.0 36.454536 4 ATCAACG 7725 0.0 36.029823 3 CAACGCA 7925 0.0 35.23987 5 AACGCAG 8260 0.0 34.08179 6 TATCAAC 8485 0.0 33.0249 2 ACGCAGA 9610 0.0 29.098406 7 CGCAGAG 9870 0.0 28.235056 8 GTACATG 7115 0.0 26.51766 1 TACATGG 7130 0.0 25.321463 2 GCAGAGT 11060 0.0 24.857182 9 ACATGGG 7375 0.0 23.71482 3 AGAGTAC 10215 0.0 20.5646 10-11 GAGTACT 7555 0.0 20.557957 12-13 CAGAGTA 10815 0.0 20.444864 10-11 CATGGGG 5065 0.0 20.14277 4 GTGGTAT 2060 0.0 19.87091 1 TGGTATC 1910 0.0 19.69263 2 GTATAGA 1215 0.0 18.975164 1 >>END_MODULE