FastQCFastQC Report
Wed 25 May 2016
SRR1033179_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033179_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3736057
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG157350.028.414751
TACATGG163550.026.9587942
ACATGGG168300.025.3171843
CATGGGG133050.023.3366284
GTATCAA94450.021.249661
GAGTACA104750.020.5994591
GGTATCA76050.020.0100251
ATGGGGC53050.019.834445
AGTACAT109450.018.8966642
TCAACGC102950.018.8440914
ATCAACG105300.018.5591133
CAACGCA105500.018.4779175
GTATATA18200.018.37941
AACGCAG107050.018.2927446
TCTATAC11200.018.03613
ATATCGT2705.501679E-717.3926476
TAGGACA9800.016.7761964
GTATAGA13200.016.7752231
TATCAAC118350.016.7607082
TATACTG19200.016.391965
Produced by FastQC (version 0.11.5)