FastQCFastQC Report
Wed 25 May 2016
SRR1033179_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033179_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3736057
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64360.17226717900717253No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57050.15270109636978238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA133250.032.600021
GGTATCA93100.032.3023831
TCAACGC159100.027.067924
GTACATG157300.026.987411
ATCAACG159450.026.9790213
CAACGCA161700.026.6323365
AACGCAG165650.026.0148566
TACATGG160450.025.8446162
TATCAAC173100.025.015192
ACATGGG169800.023.8393863
ACGCAGA187150.022.875587
CGCAGAG196050.021.7651968
CATGGGG129800.021.6961044
AGAGTAC157200.020.64186310-11
GCAGAGT205600.020.3882279
GAGTACT95100.019.54356212-13
GAGTACA109000.019.429851
GTACTTT96150.018.8902614-15
CTAGCGT2302.2731092E-618.3968834
GTATAGA11600.018.2573571
Produced by FastQC (version 0.11.5)