##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033179_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3736057 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.781471214170445 34.0 31.0 34.0 31.0 34.0 2 32.8633133809254 34.0 31.0 34.0 31.0 34.0 3 32.95309546936784 34.0 31.0 34.0 31.0 34.0 4 36.3811834241287 37.0 37.0 37.0 35.0 37.0 5 36.32276702416478 37.0 37.0 37.0 35.0 37.0 6 36.397872676996094 37.0 37.0 37.0 35.0 37.0 7 36.37118170306288 37.0 37.0 37.0 35.0 37.0 8 36.36365879856758 37.0 37.0 37.0 35.0 37.0 9 38.189599891008086 39.0 39.0 39.0 37.0 39.0 10-11 38.168990462404615 39.0 39.0 39.0 37.0 39.0 12-13 38.13179764120301 39.0 39.0 39.0 37.0 39.0 14-15 39.67352666193262 41.0 40.0 41.0 37.0 41.0 16-17 39.679122534800726 41.0 40.0 41.0 37.0 41.0 18-19 39.68506837556279 41.0 40.0 41.0 37.0 41.0 20-21 39.67030254088736 41.0 40.0 41.0 37.0 41.0 22-23 39.60264672086106 41.0 40.0 41.0 37.0 41.0 24-25 39.545665122346904 41.0 40.0 41.0 37.0 41.0 26-27 39.447938561965195 41.0 39.0 41.0 36.0 41.0 28-29 39.358577773304845 41.0 39.0 41.0 36.0 41.0 30-31 39.26632409516236 41.0 39.0 41.0 36.0 41.0 32-33 39.308045353697764 41.0 39.0 41.0 35.5 41.0 34-35 39.24490820134704 41.0 39.0 41.0 35.0 41.0 36-37 39.15866982222167 41.0 39.0 41.0 35.0 41.0 38-39 39.068478746443105 41.0 39.0 41.0 35.0 41.0 40-41 38.950311250604585 41.0 39.0 41.0 35.0 41.0 42-43 38.82266491116169 41.0 39.0 41.0 35.0 41.0 44-45 38.66594005391245 41.0 38.0 41.0 35.0 41.0 46-47 38.437875412500404 41.0 38.0 41.0 35.0 41.0 48-49 38.229271930273015 40.0 38.0 41.0 34.0 41.0 50-51 38.059438065318595 40.0 37.5 41.0 34.0 41.0 52-53 37.88800277940085 40.0 37.0 41.0 34.0 41.0 54-55 37.71576022528564 40.0 36.5 41.0 34.0 41.0 56-57 37.506215376264336 40.0 36.0 41.0 33.5 41.0 58-59 37.26834748506246 39.0 35.0 41.0 33.0 41.0 60-61 37.02483072394238 39.0 35.0 41.0 33.0 41.0 62-63 36.75249654916935 39.0 35.0 41.0 33.0 41.0 64-65 36.47071123379541 38.0 35.0 41.0 33.0 41.0 66-67 36.15620559322302 37.0 35.0 40.0 32.0 41.0 68-69 35.81679307890646 37.0 35.0 40.0 32.0 41.0 70-71 35.4796744268088 36.0 35.0 39.0 32.0 41.0 72-73 35.13835696296925 35.5 35.0 39.0 31.0 41.0 74-75 34.82678168454068 35.0 35.0 38.0 31.0 40.5 76-77 34.52477130300743 35.0 35.0 37.0 31.0 39.5 78-79 34.23147625959668 35.0 35.0 37.0 31.0 39.0 80-81 33.952758215412665 35.0 34.0 36.0 31.0 39.0 82-83 33.69416499801797 35.0 34.0 36.0 30.5 37.0 84-85 33.47811998050351 35.0 34.0 35.5 30.0 37.0 86-87 33.30583272685615 35.0 34.0 35.0 30.0 37.0 88-89 33.14159848203601 35.0 34.0 35.0 30.0 36.0 90-91 32.986055887262964 35.0 34.0 35.0 30.0 36.0 92-93 32.85634306435903 35.0 34.0 35.0 30.0 36.0 94-95 32.7318604882099 35.0 34.0 35.0 30.0 35.5 96-97 32.635890191182845 35.0 34.0 35.0 29.5 35.0 98-99 32.5532441020038 35.0 34.0 35.0 29.5 35.0 100 32.47062531433541 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06481893514303749 1101 2 0.08538929975424736 1101 3 0.09249570219896697 1101 4 0.0252713332913288 1101 5 0.0480994793532048 1101 6 0.0666038489162517 1101 7 0.09856902061054029 1101 8 0.11450396316634937 1101 9 0.12434402003258072 1101 10-11 0.10484635925364927 1101 12-13 0.15351412363768446 1101 14-15 0.21893824339790768 1101 16-17 0.25471874374893844 1101 18-19 0.24508139542792406 1101 20-21 0.24592305545159832 1101 22-23 0.2667539644876342 1101 24-25 0.16144039229681795 1101 26-27 0.17872507421942174 1101 28-29 0.21155418152009986 1101 30-31 0.20953896141263328 1101 32-33 0.17309395618151768 1101 34-35 0.19743890011915255 1101 36-37 0.2431905278581823 1101 38-39 0.22426160653216698 1101 40-41 0.23324610717148886 1101 42-43 0.15939048051734517 1101 44-45 0.14528498333957884 1101 46-47 0.24739546157488235 1101 48-49 0.2116051450943388 1101 50-51 0.30151045411289346 1101 52-53 0.33393280005861214 1101 54-55 0.3764111758605111 1101 56-57 0.3520059097487973 1101 58-59 0.3315307137441508 1101 60-61 0.32299426201117143 1101 62-63 0.26425514723572974 1101 64-65 0.27687414624403317 1101 66-67 0.264428642469241 1101 68-69 0.31225628877463407 1101 70-71 0.3322768387324757 1101 72-73 0.32530463882219607 1101 74-75 0.31139830722970885 1101 76-77 0.2695161015271168 1101 78-79 0.26531215926394225 1101 80-81 0.14020005732412244 1101 82-83 0.2154014375329183 1101 84-85 0.2782387771860826 1101 86-87 0.2188724505511459 1101 88-89 0.253692465354753 1101 90-91 0.21898220033411775 1101 92-93 0.21313940223598848 1101 94-95 0.16322818650409943 1101 96-97 0.17364956253265973 1101 98-99 0.1255819785071779 1101 100 0.14718854579867724 1102 1 0.15601040966782875 1102 2 0.17636870764163604 1102 3 0.1557364203959466 1102 4 0.06407320100230152 1102 5 0.09294551302865273 1102 6 0.09286458964931654 1102 7 0.10776918331184504 1102 8 0.15037916644769211 1102 9 0.14077457263005755 1102 10-11 0.12344746953150576 1102 12-13 0.12224513807086623 1102 14-15 0.19866057178897023 1102 16-17 0.1767852264110843 1102 18-19 0.12918322385092296 1102 20-21 0.17336383039278758 1102 22-23 0.23275143844010415 1102 24-25 0.17118733932238683 1102 26-27 0.2362216412859084 1102 28-29 0.2259186089653369 1102 30-31 0.23062790987465576 1102 32-33 0.24411943329714347 1102 34-35 0.28551256819660153 1102 36-37 0.2731900102692322 1102 38-39 0.23518292401634966 1102 40-41 0.2869925173986232 1102 42-43 0.2526970948772913 1102 44-45 0.27918194868277624 1102 46-47 0.31000966083122705 1102 48-49 0.25142879361927584 1102 50-51 0.26549842135126767 1102 52-53 0.28303823635865655 1102 54-55 0.25873161022663993 1102 56-57 0.23219687339270934 1102 58-59 0.3029705135751968 1102 60-61 0.28753579787673544 1102 62-63 0.21067439977629476 1102 64-65 0.19301943796067889 1102 66-67 0.22169321305565148 1102 68-69 0.21924429610899665 1102 70-71 0.23684370749005978 1102 72-73 0.23868706128789796 1102 74-75 0.21887958674619767 1102 76-77 0.2113799042828859 1102 78-79 0.24779253547717417 1102 80-81 0.2604110064572538 1102 82-83 0.18134795298534812 1102 84-85 0.24691376394906683 1102 86-87 0.23921876558459587 1102 88-89 0.270025217270792 1102 90-91 0.22155204092128855 1102 92-93 0.25148292930207816 1102 94-95 0.27162655894220933 1102 96-97 0.2658287439005562 1102 98-99 0.29532428476463934 1102 100 0.21168466494872007 1103 1 0.12429342484855965 1103 2 0.1477457046380195 1103 3 0.07964638202334129 1103 4 0.0674416762758625 1103 5 0.05707497905415693 1103 6 0.09579477907239209 1103 7 0.12197054854968314 1103 8 0.12414753237571574 1103 9 0.1366390971181488 1103 10-11 0.12167964129437081 1103 12-13 0.11253789720455387 1103 14-15 0.1432778454476491 1103 16-17 0.12622195002101932 1103 18-19 0.1442978728777149 1103 20-21 0.1557826938543201 1103 22-23 0.1105730448029334 1103 24-25 0.10872256880682585 1103 26-27 0.1420602187998128 1103 28-29 0.121741564073794 1103 30-31 0.19321151459503483 1103 32-33 0.21013533584406474 1103 34-35 0.21044764428098972 1103 36-37 0.17401992937838173 1103 38-39 0.16538703192955495 1103 40-41 0.21287194134642817 1103 42-43 0.1611891091509534 1103 44-45 0.17648129452984307 1103 46-47 0.15773921586942663 1103 48-49 0.09461565136952999 1103 50-51 0.054348000065935764 1103 52-53 0.08922891103646435 1103 54-55 0.08009454366932545 1103 56-57 0.11173434626812906 1103 58-59 0.1503289941240027 1103 60-61 0.13168112352425254 1103 62-63 0.049978813021112956 1103 64-65 0.0648750476242057 1103 66-67 0.08274191361716987 1103 68-69 0.06282199639602482 1103 70-71 0.14965532251692792 1103 72-73 0.18926976684452512 1103 74-75 0.1620351545014671 1103 76-77 0.10556267626015625 1103 78-79 0.09032027004764132 1103 80-81 0.133582603274931 1103 82-83 0.11740848613523269 1103 84-85 0.10913442731955314 1103 86-87 0.08522285248719186 1103 88-89 0.04021488886139224 1103 90-91 0.0662333630534846 1103 92-93 0.08961594453928257 1103 94-95 0.15203947391587747 1103 96-97 0.12349597875737572 1103 98-99 0.08068847432552673 1103 100 0.0627296039332279 1104 1 0.0502118425694249 1104 2 0.09776733765384904 1104 3 0.09567483561778545 1104 4 0.055561505977259174 1104 5 0.015894351349444946 1104 6 0.053258607512411515 1104 7 0.06070515946667143 1104 8 0.09193858403435229 1104 9 0.12961986391236024 1104 10-11 0.1266054930767737 1104 12-13 0.1366273474569013 1104 14-15 0.12542614939105334 1104 16-17 0.13029280495217677 1104 18-19 0.13159305138998434 1104 20-21 0.1295782760890276 1104 22-23 0.16207745446561717 1104 24-25 0.12821308703878032 1104 26-27 0.08177073995370421 1104 28-29 0.12168228053800334 1104 30-31 0.09469307115857362 1104 32-33 0.10021912428411639 1104 34-35 0.12023859395317515 1104 36-37 0.12369681064519256 1104 38-39 0.116034035858533 1104 40-41 0.11538149631946482 1104 42-43 0.11139203403480735 1104 44-45 0.13724967172204572 1104 46-47 0.11618130086043266 1104 48-49 0.03831928624802572 1104 50-51 0.01612982852034861 1104 52-53 0.023104618332844495 1104 54-55 -0.002631485369285258 1104 56-57 0.10124773011290955 1104 58-59 0.09908377395683488 1104 60-61 0.08253569803895999 1104 62-63 0.12219679623589741 1104 64-65 0.124756580662023 1104 66-67 0.0869113137397477 1104 68-69 0.11329308933728299 1104 70-71 0.06790285740114399 1104 72-73 0.09169610587815669 1104 74-75 0.11499327780057911 1104 76-77 0.0584627673909921 1104 78-79 0.0689613037022383 1104 80-81 0.17526127524865132 1104 82-83 0.13902851902743407 1104 84-85 0.1364073565174877 1104 86-87 0.14049184488294486 1104 88-89 0.15765823241526533 1104 90-91 0.16210156652459062 1104 92-93 0.1329340643721153 1104 94-95 0.07475877059728475 1104 96-97 0.11264380686829156 1104 98-99 0.14767230008762056 1104 100 0.14892098244906293 1105 1 0.02826736441817701 1105 2 0.03591130615821214 1105 3 0.03571357597202507 1105 4 0.059539807783494325 1105 5 -0.014994564504888785 1105 6 0.012584830957067084 1105 7 -0.04368526420539354 1105 8 -0.036218282940616575 1105 9 0.04322797511785126 1105 10-11 0.01878955919604408 1105 12-13 0.027637517923452037 1105 14-15 0.013236420528592419 1105 16-17 0.04617814788520036 1105 18-19 0.049063962417498885 1105 20-21 0.05204678269781482 1105 22-23 0.10817757715838638 1105 24-25 0.05635647129185628 1105 26-27 0.07486488980068629 1105 28-29 0.07527924602673153 1105 30-31 0.0386089929048552 1105 32-33 0.02001535541392485 1105 34-35 0.03528081734390298 1105 36-37 -0.018375124170340484 1105 38-39 -0.007355340655458065 1105 40-41 0.059857400448329656 1105 42-43 0.05118179345809892 1105 44-45 0.07589205151234779 1105 46-47 0.07047757019128653 1105 48-49 -0.054662058159117066 1105 50-51 -0.07578053834451026 1105 52-53 -0.09029451068332861 1105 54-55 -0.07881380772318636 1105 56-57 -0.1184073818610969 1105 58-59 -0.11882577495622826 1105 60-61 -0.18354042540884308 1105 62-63 -0.1467291251957903 1105 64-65 -0.19365632320167236 1105 66-67 -0.2232290413951432 1105 68-69 -0.20240484376145673 1105 70-71 -0.1105387197177734 1105 72-73 -0.11240578754541986 1105 74-75 -0.1512401440692912 1105 76-77 -0.2135093503401393 1105 78-79 -0.11594883395635236 1105 80-81 -0.08595612465337155 1105 82-83 -0.05963848920152515 1105 84-85 -0.10371542613653872 1105 86-87 -0.06595064906106529 1105 88-89 -0.10072599819320516 1105 90-91 -0.12287238935095246 1105 92-93 -0.057625333759176556 1105 94-95 -0.12096945368329415 1105 96-97 -0.0918513326104673 1105 98-99 -0.06830427593021682 1105 100 -0.06330245321812811 1106 1 -0.02993953279163719 1106 2 -0.014529550363029387 1106 3 -0.029348067602157357 1106 4 0.0023224521592339897 1106 5 -0.02282899202756994 1106 6 -0.015655591705375116 1106 7 -0.04439664631921403 1106 8 -0.007347588130386384 1106 9 0.0034309726877381763 1106 10-11 0.0013257883142046012 1106 12-13 0.01877808308497464 1106 14-15 0.042929983896151214 1106 16-17 0.002358727296638108 1106 18-19 0.010284492463178196 1106 20-21 -0.02335129229233246 1106 22-23 0.035451439392303996 1106 24-25 0.020633256665348654 1106 26-27 -0.02152810229825519 1106 28-29 -0.03547036909852608 1106 30-31 0.07165231834984809 1106 32-33 0.024468849185950603 1106 34-35 0.02115710715141006 1106 36-37 0.007706421562950538 1106 38-39 0.024670690872461876 1106 40-41 0.03603723237575451 1106 42-43 0.020123594671886735 1106 44-45 0.0979435452080395 1106 46-47 0.07671460850063028 1106 48-49 -7.911247901617458E-4 1106 50-51 0.020391627895925524 1106 52-53 -0.014657081773826519 1106 54-55 -0.049691990096555116 1106 56-57 -0.004087973995659411 1106 58-59 -0.04998170329955087 1106 60-61 -0.004817637007242581 1106 62-63 -0.008919953089986166 1106 64-65 0.040644683834216266 1106 66-67 0.0375167567905379 1106 68-69 0.061998457255121764 1106 70-71 0.06054263516854519 1106 72-73 0.11067007730947154 1106 74-75 0.06266981750684408 1106 76-77 -0.002884739986050988 1106 78-79 0.03634387441673681 1106 80-81 0.04581162729644461 1106 82-83 0.07324810316205799 1106 84-85 0.05868151649485753 1106 86-87 0.055896320288908896 1106 88-89 0.008505667773690107 1106 90-91 -0.022541110803423692 1106 92-93 -0.05645822496367714 1106 94-95 -0.03820826368877306 1106 96-97 -0.01663662975921909 1106 98-99 -0.021176125364675613 1106 100 -5.217491200042446E-4 1107 1 0.0036814354420044992 1107 2 -0.012801606944947252 1107 3 0.01841304452515402 1107 4 0.018920373017721204 1107 5 -0.08702309604561265 1107 6 -0.08051246018900571 1107 7 -0.09618236060492791 1107 8 -0.03901090134299068 1107 9 -0.044506043347873 1107 10-11 -0.04019728954690294 1107 12-13 -0.004238501269639983 1107 14-15 0.02101760050489787 1107 16-17 0.003882719736118645 1107 18-19 -0.033595149022097814 1107 20-21 -0.028795320095511556 1107 22-23 -6.688429075509816E-4 1107 24-25 -0.004390369942790073 1107 26-27 0.026042167807226235 1107 28-29 -0.024202915894456112 1107 30-31 0.0068366821081511375 1107 32-33 0.07275576766653558 1107 34-35 0.07443777601305612 1107 36-37 0.019922368761299936 1107 38-39 -0.01344832626064374 1107 40-41 0.026820644972552543 1107 42-43 0.024597483459160685 1107 44-45 0.01488664318986821 1107 46-47 -0.003330663129140987 1107 48-49 0.06983635219295081 1107 50-51 0.008395484368087125 1107 52-53 0.021897091752187237 1107 54-55 -0.011951455982789128 1107 56-57 -0.020646263124369568 1107 58-59 -0.003903262960548659 1107 60-61 0.06734168254643436 1107 62-63 0.032747170865874864 1107 64-65 -0.013445386893174316 1107 66-67 0.019942980801261 1107 68-69 0.0417057159588623 1107 70-71 0.06320733549169688 1107 72-73 0.03902818047391321 1107 74-75 0.06913550557464987 1107 76-77 0.08417504295401557 1107 78-79 0.05509212147484277 1107 80-81 0.05428367168398296 1107 82-83 0.14323179318095924 1107 84-85 0.08283720822097251 1107 86-87 0.08505909999746564 1107 88-89 0.057974831239327784 1107 90-91 0.09881932453588149 1107 92-93 0.128361831740186 1107 94-95 0.08318944071950085 1107 96-97 0.1713732476872849 1107 98-99 0.14600842130931113 1107 100 0.15373012576566936 1108 1 0.009627632102485961 1108 2 -0.002059491917115963 1108 3 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-0.060574426462963515 2214 20-21 -0.11873336181983518 2214 22-23 -0.12415075566230627 2214 24-25 -0.1112791393038961 2214 26-27 -0.12017108253184716 2214 28-29 -0.11941395314469361 2214 30-31 -0.10672422847457597 2214 32-33 -0.07516325819115366 2214 34-35 -0.11284038475545799 2214 36-37 -0.150083612189988 2214 38-39 -0.16404789951907617 2214 40-41 -0.08118303119110237 2214 42-43 -0.09167064929332014 2214 44-45 -0.13208246526517087 2214 46-47 -0.12612728478128332 2214 48-49 -0.24605811481121975 2214 50-51 -0.15497804588498099 2214 52-53 -0.19908920406813024 2214 54-55 -0.17562136495462255 2214 56-57 -0.18428778652877753 2214 58-59 -0.2830989413468714 2214 60-61 -0.3513562903627232 2214 62-63 -0.3623078826273698 2214 64-65 -0.22127707991298706 2214 66-67 -0.3583609199559987 2214 68-69 -0.30597434557513736 2214 70-71 -0.15114835729270482 2214 72-73 -0.24555459684993508 2214 74-75 -0.227306854889207 2214 76-77 -0.22353954933534936 2214 78-79 -0.34733559729178864 2214 80-81 -0.23961404720583346 2214 82-83 -0.24994830735924722 2214 84-85 -0.2957297996473329 2214 86-87 -0.25952853599390835 2214 88-89 -0.37087244646978235 2214 90-91 -0.23624261321021578 2214 92-93 -0.18523096812514694 2214 94-95 -0.23280392411989226 2214 96-97 -0.4008845135208432 2214 98-99 -0.4639619066028473 2214 100 -0.6714241950952591 2215 1 -0.05654585545215696 2215 2 -0.08514316029977209 2215 3 -0.09382439604435433 2215 4 -0.05526360669350083 2215 5 -0.06343938335462695 2215 6 -0.09883126331476433 2215 7 -0.09439473879594118 2215 8 -0.07911699597067923 2215 9 -0.09397248514149226 2215 10-11 -0.08268669494794523 2215 12-13 -0.16025576443782796 2215 14-15 -0.19338512175377076 2215 16-17 -0.15105702895306194 2215 18-19 -0.12812700812896338 2215 20-21 -0.09987706911103089 2215 22-23 -0.13076429504176446 2215 24-25 -0.14862066530996998 2215 26-27 -0.12286283125800423 2215 28-29 -0.1191753649825742 2215 30-31 -0.1026493535002686 2215 32-33 -0.1605856119467859 2215 34-35 -0.1547029037456582 2215 36-37 -0.11008436289657908 2215 38-39 -0.1263672167195864 2215 40-41 -0.18913824633198573 2215 42-43 -0.13423889761742203 2215 44-45 -0.11997609110518681 2215 46-47 -0.15393867254686455 2215 48-49 -0.052020819070598634 2215 50-51 0.009946500623819077 2215 52-53 -0.05513897051201866 2215 54-55 -0.02687040853945888 2215 56-57 -0.14120372895575173 2215 58-59 -0.1735756974491025 2215 60-61 -0.06360530118858065 2215 62-63 -0.127134301748697 2215 64-65 -0.07921949782218007 2215 66-67 -0.1151276883942387 2215 68-69 -0.03617895799173709 2215 70-71 0.024594915814112994 2215 72-73 0.038418724311277686 2215 74-75 -0.0014643254402599837 2215 76-77 -0.10509357133520325 2215 78-79 -0.05647147947987463 2215 80-81 -0.060102984439282636 2215 82-83 -0.01513645030555466 2215 84-85 -0.0025813337640272493 2215 86-87 -0.08634413027346 2215 88-89 -0.1305804915598472 2215 90-91 -0.15231977899754412 2215 92-93 -0.10066589621627742 2215 94-95 -0.04532411805500658 2215 96-97 -0.04357182555170169 2215 98-99 -0.12601853438924593 2215 100 -0.10024883895951575 2216 1 -0.09546728011389405 2216 2 -0.1443768979214326 2216 3 -0.1483199829264379 2216 4 -0.09378849496061292 2216 5 -0.07624996697907704 2216 6 -0.06898590291394413 2216 7 -0.07980083793597714 2216 8 -0.13652444568288047 2216 9 -0.1808209460100798 2216 10-11 -0.16376761642560922 2216 12-13 -0.1406266989003555 2216 14-15 -0.28147753908605466 2216 16-17 -0.2520321388583824 2216 18-19 -0.1841449584940662 2216 20-21 -0.19001022794215316 2216 22-23 -0.21198730986972691 2216 24-25 -0.2502995256573328 2216 26-27 -0.3310446326178962 2216 28-29 -0.37542145284652406 2216 30-31 -0.3287066468184179 2216 32-33 -0.2468840436199855 2216 34-35 -0.23324588264365786 2216 36-37 -0.34711615160058074 2216 38-39 -0.3142472478978817 2216 40-41 -0.3490491090259482 2216 42-43 -0.28322621978369966 2216 44-45 -0.19686595550884078 2216 46-47 -0.24848464088790934 2216 48-49 -0.25797095527004643 2216 50-51 -0.2601752313433465 2216 52-53 -0.2065648156019435 2216 54-55 -0.1329624210656064 2216 56-57 -0.13003994329648805 2216 58-59 -0.18344294982059495 2216 60-61 -0.1366722376650884 2216 62-63 -0.15406628015416146 2216 64-65 -0.17327378205092003 2216 66-67 -0.14514327525804305 2216 68-69 -0.12905587773686023 2216 70-71 -0.12344941594050596 2216 72-73 -0.049339305333695904 2216 74-75 -0.09039219227639705 2216 76-77 -0.10868721953399785 2216 78-79 -0.09795548461168835 2216 80-81 -0.031932532271746084 2216 82-83 -0.043828307397106414 2216 84-85 -0.05301621480310814 2216 86-87 -0.09842127691105418 2216 88-89 -0.1392851941644082 2216 90-91 -0.13061861776134265 2216 92-93 -0.09605666892083065 2216 94-95 -0.18315903116788945 2216 96-97 -0.18944699494433337 2216 98-99 -0.1817642800412358 2216 100 -0.19711869607117904 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23.0 3 5.0 4 20.0 5 61.0 6 74.0 7 238.0 8 475.0 9 825.0 10 1296.0 11 2138.0 12 3145.0 13 4759.0 14 5336.0 15 6838.0 16 9000.0 17 13834.0 18 16531.0 19 8979.0 20 6838.0 21 6806.0 22 7115.0 23 7571.0 24 8542.0 25 10541.0 26 13338.0 27 17034.0 28 21709.0 29 28142.0 30 36112.0 31 46328.0 32 59874.0 33 79800.0 34 114287.0 35 189941.0 36 404698.0 37 890484.0 38 1240656.0 39 471564.0 40 1100.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.7399050062596 18.64299708492614 13.328646782581998 24.288451126232264 2 16.162403205558874 20.12374163880718 38.59523930610464 25.118615849529313 3 18.403787736643203 24.93227485554958 29.929575485598853 26.73436192220836 4 12.990862807206222 16.661772905012757 36.97724521279031 33.37011907499072 5 13.750121832489166 37.589366139029345 34.45037213800756 14.210139890473931 6 31.855507325783293 36.47934677551243 17.553882428264497 14.111263470439777 7 28.593500143740542 32.04180092711311 21.7663597881955 17.59833914095085 8 26.883066513884863 34.50641814730918 19.73476243741045 18.87575290139551 9 26.700734760563773 15.479328820006815 19.93026318994381 37.8896732294856 10-11 25.495895458418655 25.288376516928533 27.810991009341564 21.404737015311248 12-13 26.13307291617874 22.862392088771667 28.043000949931972 22.96153404511762 14-15 23.322431009084855 24.2576558392432 25.617807667904113 26.80210548376783 16-17 22.96364857388418 27.575221684251606 26.054206346423513 23.4069233954407 18-19 22.98973837651256 26.404957527403226 27.946703689666325 22.658600406417893 20-21 24.053688153044774 25.464654313357638 27.625649180405976 22.856008353191616 22-23 23.875202419667833 25.369534668968562 27.443369333922192 23.311893577441413 24-25 23.56344028891457 25.333075485422114 27.673634136565266 23.429850089098053 26-27 23.394433407690897 25.630727713743195 27.69365487491709 23.281184003648818 28-29 23.113254202765756 25.908856501596944 27.713044685174186 23.26484461046311 30-31 23.39781076623182 25.66702546965705 27.55008514876936 23.385078615341772 32-33 23.64079416027595 25.514937659963167 27.64289373646517 23.201374443295713 34-35 23.675818868021523 25.40304831813976 27.481597960005484 23.439534853833237 36-37 23.890157812034392 25.41866654814856 27.361082812830904 23.330092826986146 38-39 23.607565147268758 25.745232493261184 27.330113229289044 23.317089130181017 40-41 23.47340344067918 25.704527418012642 27.46434656976302 23.357722571545157 42-43 23.663338675842237 25.689864346323088 27.142609112143557 23.50418786569112 44-45 23.554858937526323 25.91313317746438 27.014027938077568 23.517979946931735 46-47 23.673569258058652 25.912157468283 27.033064495187602 23.381208778470747 48-49 23.810872466764714 25.84778021853295 26.44437175122429 23.896975563478044 50-51 23.55115317370208 26.052494602497404 26.631159346482924 23.76519287731759 52-53 23.612982993655244 26.10258305961094 26.674676465085106 23.60975748164871 54-55 23.5961772057434 26.112527991871314 26.635961932661047 23.655332869724237 56-57 23.364373535433916 26.002891089750257 26.764355039525974 23.868380335289856 58-59 23.624495636466662 25.733223358918934 26.751686721205093 23.89059428340931 60-61 23.72990984557421 25.9402216360986 26.62170633350767 23.708162184819518 62-63 23.373132434728564 26.02057968733522 26.78837659601918 23.817911281917038 64-65 23.55346267580045 26.091895027968135 26.580164737325674 23.77447755890574 66-67 23.358971486738543 25.888445593434856 26.6943939341015 24.058188985725103 68-69 23.50252956557746 25.97593908164765 26.607855371107785 23.913675981667108 70-71 23.527320843369026 25.876640222888437 26.580232891006638 24.015806042735896 72-73 23.466173880155768 25.898526592243698 26.548601149936307 24.086698377664224 74-75 23.657727327354976 26.006060438973638 26.323161492417224 24.013050741254162 76-77 23.607385202267054 25.828012746656654 26.46468980445464 24.099912246621656 78-79 23.563601655168572 26.02219801665099 26.366264196627753 24.047936131552685 80-81 23.387360336485248 26.09017372169892 26.557072702516276 23.965393239299544 82-83 23.543520764039236 25.8699957187272 26.56804794704337 24.01843557019019 84-85 23.507411451099465 25.816739796624393 26.34474496144718 24.33110379082896 86-87 23.589703509014 26.00392473386661 26.44279739869614 23.963574358423248 88-89 23.293799223860773 25.980696491072873 26.34629588712055 24.3792083979458 90-91 23.28129537707555 25.781753748271907 26.392384480421192 24.54456639423135 92-93 23.338983676904203 25.762394889155836 26.507030408537773 24.39159102540219 94-95 23.292350732336256 25.663593997629054 26.559873149686958 24.484182120347736 96-97 23.222076108581856 25.546157352524336 26.59339779880232 24.638368740091494 98-99 23.1049472746267 25.814327779260328 26.483683198623574 24.5970417474894 100 23.18666444328874 25.48261442477992 26.5006128118495 24.83010832008184 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 77.0 1 66.5 2 194.5 3 370.5 4 270.5 5 196.0 6 445.5 7 968.0 8 1689.0 9 2085.5 10 1696.5 11 1260.5 12 1310.5 13 1476.0 14 1648.0 15 1848.5 16 2092.0 17 2347.5 18 2613.0 19 2849.5 20 3263.5 21 3825.5 22 4564.5 23 5766.0 24 7384.5 25 9624.5 26 12993.5 27 17865.5 28 24089.0 29 30675.5 30 36538.0 31 43081.0 32 50573.5 33 57062.0 34 63451.5 35 69865.0 36 77394.5 37 86970.0 38 93638.0 39 98126.5 40 104311.5 41 112047.5 42 113696.0 43 113251.0 44 116975.5 45 117620.0 46 121244.0 47 125305.5 48 128568.5 49 129480.0 50 126030.0 51 127529.0 52 129637.0 53 133961.5 54 135481.5 55 129578.5 56 120236.0 57 108756.5 58 103352.5 59 100738.5 60 93237.0 61 82607.5 62 72764.5 63 61766.5 64 48982.0 65 39239.5 66 30793.0 67 22669.5 68 17368.5 69 13305.0 70 9230.0 71 6435.0 72 5130.0 73 3984.5 74 3055.0 75 2216.0 76 1360.0 77 895.0 78 528.5 79 249.0 80 125.5 81 48.0 82 27.5 83 12.5 84 4.0 85 0.5 86 0.5 87 1.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09073737365356042 2 0.0012580107851673569 3 0.0 4 2.1412949534763522E-4 5 0.038034751611123706 6 1.605971215107264E-4 7 0.00425582372003425 8 2.6766186918454404E-4 9 2.6766186918454404E-4 10-11 3.3457733648068003E-4 12-13 0.0 14-15 9.36816542145904E-5 16-17 0.0 18-19 2.6766186918454403E-5 20-21 0.0 22-23 1.873633084291808E-4 24-25 1.2044784113304481E-4 26-27 0.0014721402805149921 28-29 0.00889975715038609 30-31 0.03792768686344988 32-33 0.027234595189527356 34-35 0.03064728402163029 36-37 0.027943899142866397 38-39 0.026257629367003772 40-41 0.03246738473208519 42-43 0.024638275058437277 44-45 0.02323305024521842 46-47 0.016059712151072644 48-49 0.011215032318832394 50-51 0.008070005355914003 52-53 0.005781496374386151 54-55 0.004041694224686615 56-57 0.0025561708507123955 58-59 9.769658225235857E-4 60-61 6.156222991244513E-4 62-63 0.0011509460374935392 64-65 0.002930897467570757 66-67 6.290053925836784E-4 68-69 0.0010706474767381762 70-71 4.684082710729521E-4 72-73 6.423884860429056E-4 74-75 6.423884860429056E-4 76-77 4.951744579914064E-4 78-79 6.691546729613601E-5 80-81 6.423884860429056E-4 82-83 4.951744579914064E-4 84-85 3.747266168583616E-4 86-87 2.00746401888408E-4 88-89 1.3383093459227201E-5 90-91 5.3532373836908805E-5 92-93 2.6766186918454403E-5 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3736057.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.039005973361018 #Duplication Level Percentage of deduplicated Percentage of total 1 53.207688937481 13.322676411343219 2 14.96936846328265 7.496362127391526 3 8.024274906318979 6.027596019336355 4 5.023994931053325 5.031833563551187 5 3.5127238141844987 4.3977556283066574 6 2.526473118791986 3.7956225307781146 7 1.9198200255604327 3.364926956145004 8 1.4878820897111493 2.980407082554747 9 1.1879420839511825 2.6770400042454536 >10 7.54278687921765 34.37877081160848 >50 0.4226660994616655 7.176792669500373 >100 0.16623461768251013 7.28709761835264 >500 0.006000866645541731 1.0119992117311596 >1k 0.0019288499917575138 0.7253595639465734 >5k 2.1431666575083487E-4 0.32575980120867604 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6436 0.17226717900717253 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5705 0.15270109636978238 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.6766186918454403E-5 0.0 14-15 0.0 0.0 0.0 2.6766186918454403E-5 0.0 16-17 0.0 0.0 0.0 5.3532373836908805E-5 0.0 18-19 0.0 0.0 0.0 1.0706474767381761E-4 0.0 20-21 0.0 0.0 0.0 1.4721402805149922E-4 0.0 22-23 0.0 0.0 0.0 2.542787757253168E-4 0.0 24-25 0.0 0.0 0.0 5.35323738369088E-4 0.0 26-27 0.0 0.0 0.0 9.635827290643585E-4 0.0 28-29 0.0 0.0 0.0 0.0027033848787638946 0.0 30-31 0.0 0.0 0.0 0.009046971178437588 0.0 32-33 0.0 0.0 0.0 0.021305884787089703 0.0 34-35 1.3383093459227201E-5 0.0 0.0 0.03986823541503783 0.0 36-37 2.6766186918454403E-5 0.0 0.0 0.07372746186688266 0.0 38-39 2.6766186918454403E-5 0.0 0.0 0.12435570442313916 0.0 40-41 2.6766186918454403E-5 0.0 0.0 0.18095280666221097 0.0 42-43 2.6766186918454403E-5 0.0 0.0 0.2360108531534717 0.0 44-45 2.6766186918454403E-5 0.0 0.0 0.3058706010106377 0.0 46-47 2.6766186918454403E-5 0.0 0.0 0.3925796635329707 0.0 48-49 2.6766186918454403E-5 0.0 0.0 0.4827683303547028 0.0 50-51 2.6766186918454403E-5 0.0 0.0 0.578618045709688 0.0 52-53 2.6766186918454403E-5 0.0 0.0 0.6767696531396603 0.0 54-55 2.6766186918454403E-5 0.0 0.0 0.7840217641219072 0.0 56-57 2.6766186918454403E-5 0.0 0.0 0.9150288659942822 0.0 58-59 2.6766186918454403E-5 0.0 0.0 1.0610250325409916 0.0 60-61 2.6766186918454403E-5 0.0 0.0 1.211062893312388 0.0 62-63 2.6766186918454403E-5 0.0 0.0 1.3648881695327453 0.0 64-65 2.6766186918454403E-5 0.0 0.0 1.5279745464268881 0.0 66-67 2.6766186918454403E-5 0.0 0.0 1.7154181534168242 0.0 68-69 2.6766186918454403E-5 0.0 0.0 1.9253855066986398 0.0 70-71 2.6766186918454403E-5 0.0 0.0 2.1517605325614677 0.0 72-73 2.6766186918454403E-5 0.0 0.0 2.384211482854785 0.0 74-75 2.6766186918454403E-5 0.0 0.0 2.6281986597099563 0.0 76-77 2.6766186918454403E-5 0.0 0.0 2.8924076907820195 0.0 78-79 2.6766186918454403E-5 0.0 0.0 3.1897532612591295 0.0 80-81 2.6766186918454403E-5 0.0 0.0 3.5117772560750544 0.0 82-83 2.6766186918454403E-5 0.0 0.0 3.8468230008268076 0.0 84-85 2.6766186918454403E-5 0.0 0.0 4.18724874914917 0.0 86-87 2.6766186918454403E-5 0.0 0.0 4.545018986594691 0.0 88 2.6766186918454403E-5 0.0 0.0 4.831082609285672 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13325 0.0 32.60002 1 GGTATCA 9310 0.0 32.302383 1 TCAACGC 15910 0.0 27.06792 4 GTACATG 15730 0.0 26.98741 1 ATCAACG 15945 0.0 26.979021 3 CAACGCA 16170 0.0 26.632336 5 AACGCAG 16565 0.0 26.014856 6 TACATGG 16045 0.0 25.844616 2 TATCAAC 17310 0.0 25.01519 2 ACATGGG 16980 0.0 23.839386 3 ACGCAGA 18715 0.0 22.87558 7 CGCAGAG 19605 0.0 21.765196 8 CATGGGG 12980 0.0 21.696104 4 AGAGTAC 15720 0.0 20.641863 10-11 GCAGAGT 20560 0.0 20.388227 9 GAGTACT 9510 0.0 19.543562 12-13 GAGTACA 10900 0.0 19.42985 1 GTACTTT 9615 0.0 18.89026 14-15 CTAGCGT 230 2.2731092E-6 18.396883 4 GTATAGA 1160 0.0 18.257357 1 >>END_MODULE