FastQCFastQC Report
Wed 25 May 2016
SRR1033178_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033178_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2038543
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT48490.237865966035546No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36310.17811741032688544No Hit
GTACACAGGAGGCAAAGTGTTTCACATCATAGACTTCACTTCCAACTCCT30540.14981288106260204No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26110.12808167401914014No Hit
GTACAAGAGTTACAGCCCTTATGACATGTTGGAAAGCATCAGGAAAGAGG25740.1262666522118984No Hit
CTCTTGTACCTATCAAATACTTTCTGGAGGTGGGGCACGCTCCGCTCGGT24460.11998765785171076No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATG550.002727554634.1560759
GTACATG92850.028.2527311
TAGCGAA350.00887857326.83231242-43
GTTCTAA6550.026.6040421
TACATGG95550.026.5175782
GTATATA3850.025.6889131
GTATAGA3600.024.8564011
GTACACA13000.024.2727281
ACATGGG101550.023.5071333
TATTAGG4300.022.957612
CATGGGG69250.022.3217374
TGACACA9600.021.5249166
ATGGGGC31050.021.3363485
ACCTATC15000.021.2906238
GTACCTA14400.020.8726466
GTATTAG6600.020.6938481
GACTGCG1857.233053E-620.308527
GACCGTG6500.020.230417
TAGACGA705.247131E-420.1237452-53
GGACCGA3800.019.7740866
Produced by FastQC (version 0.11.5)