##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033178_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2038543
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63541068302214	33.0	31.0	34.0	30.0	34.0
2	31.807087709211924	34.0	31.0	34.0	30.0	34.0
3	31.943738248346982	34.0	31.0	34.0	30.0	34.0
4	35.31635879154867	37.0	35.0	37.0	33.0	37.0
5	35.440720651955836	37.0	35.0	37.0	35.0	37.0
6	35.54520508029509	37.0	37.0	37.0	35.0	37.0
7	35.55758745339196	37.0	37.0	37.0	35.0	37.0
8	35.53667398725462	37.0	37.0	37.0	35.0	37.0
9	37.240914712125274	39.0	39.0	39.0	35.0	39.0
10-11	37.23657165926841	39.0	38.0	39.0	35.0	39.0
12-13	37.21530181114649	39.0	38.0	39.0	35.0	39.0
14-15	38.713609671221064	41.0	39.0	41.0	36.0	41.0
16-17	38.71631675171924	41.0	39.0	41.0	36.0	41.0
18-19	38.69029056536948	41.0	39.0	41.0	35.0	41.0
20-21	38.70315784361674	41.0	39.0	41.0	35.0	41.0
22-23	38.677314631087	41.0	39.0	41.0	35.0	41.0
24-25	38.64708495234096	41.0	39.0	41.0	35.0	41.0
26-27	38.563310903915195	41.0	39.0	41.0	34.0	41.0
28-29	38.50611294439214	40.5	39.0	41.0	34.0	41.0
30-31	38.47877920652152	41.0	39.0	41.0	34.0	41.0
32-33	38.52411918708607	41.0	39.0	41.0	35.0	41.0
34-35	38.47042544601708	41.0	39.0	41.0	34.0	41.0
36-37	38.37496560043129	41.0	39.0	41.0	34.0	41.0
38-39	38.27447005042327	41.0	39.0	41.0	34.0	41.0
40-41	38.1920481932439	41.0	38.0	41.0	34.0	41.0
42-43	38.05644619711235	40.0	38.0	41.0	33.0	41.0
44-45	37.901931183202905	40.0	38.0	41.0	33.0	41.0
46-47	37.73407968338171	40.0	38.0	41.0	33.0	41.0
48-49	37.57276005460763	40.0	37.0	41.0	33.0	41.0
50-51	37.38779755933527	40.0	37.0	41.0	32.5	41.0
52-53	37.196163387282	40.0	36.0	41.0	32.0	41.0
54-55	36.97242883765513	40.0	36.0	41.0	32.0	41.0
56-57	36.74346996850201	39.0	35.0	41.0	31.0	41.0
58-59	36.478085328590076	39.0	35.0	41.0	31.0	41.0
60-61	36.176956286916685	38.5	35.0	41.0	31.0	41.0
62-63	35.86151726993249	37.5	35.0	40.0	31.0	41.0
64-65	35.547199642097326	37.0	35.0	40.0	30.0	41.0
66-67	35.19100480097795	36.5	35.0	39.5	30.0	41.0
68-69	34.83522766014747	36.0	34.5	39.0	30.0	41.0
70-71	34.46842794093625	35.5	34.0	39.0	29.5	41.0
72-73	34.0913731032409	35.0	34.0	37.5	29.0	40.0
74-75	33.748527992787004	35.0	34.0	37.0	29.0	39.0
76-77	33.44771241028519	35.0	34.0	36.5	29.0	39.0
78-79	33.12206463145492	35.0	34.0	36.0	28.5	38.5
80-81	32.83177568488867	35.0	34.0	36.0	28.0	37.0
82-83	32.689811301503084	35.0	34.0	35.0	28.0	37.0
84-85	32.50231292643815	35.0	34.0	35.0	27.5	36.5
86-87	32.32135108261146	35.0	33.5	35.0	27.0	36.0
88-89	32.020565178168916	35.0	33.0	35.0	27.0	36.0
90-91	31.869678000415	35.0	33.0	35.0	26.0	36.0
92-93	31.704567919342395	35.0	33.0	35.0	25.5	35.0
94-95	31.549270238596883	35.0	33.0	35.0	25.0	35.0
96-97	31.385692624585303	35.0	33.0	35.0	25.0	35.0
98-99	31.19255566353028	35.0	33.0	35.0	24.0	35.0
100	31.014000195237482	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22967175002392182
1101	2	0.2849055710662327
1101	3	0.30838105145426553
1101	4	0.21518072144650802
1101	5	0.19531093028015079
1101	6	0.26797093959752516
1101	7	0.24410713654840066
1101	8	0.25249986845275885
1101	9	0.30387321423538083
1101	10-11	0.35270804028638736
1101	12-13	0.4406383683466757
1101	14-15	0.494927349189652
1101	16-17	0.39295098140662077
1101	18-19	0.3946745555697433
1101	20-21	0.38104556077782803
1101	22-23	0.3534522476905124
1101	24-25	0.3700026659679878
1101	26-27	0.40527824548014735
1101	28-29	0.4564629215714575
1101	30-31	0.4631827436154623
1101	32-33	0.46654116713151694
1101	34-35	0.46442785678706144
1101	36-37	0.41089163762996606
1101	38-39	0.3683006537094826
1101	40-41	0.4743049773149153
1101	42-43	0.45013734301522135
1101	44-45	0.4449852477918341
1101	46-47	0.4215986552949502
1101	48-49	0.3485613859385168
1101	50-51	0.34394371239877586
1101	52-53	0.43522442481604173
1101	54-55	0.470782292332693
1101	56-57	0.454456809353303
1101	58-59	0.4541651524324877
1101	60-61	0.35517439898927705
1101	62-63	0.369105517480115
1101	64-65	0.27198875296269165
1101	66-67	0.3237468086846462
1101	68-69	0.40783507138804254
1101	70-71	0.43494251409406104
1101	72-73	0.5278574882758633
1101	74-75	0.4323165523860837
1101	76-77	0.370282865457888
1101	78-79	0.4002940500524659
1101	80-81	0.4335033528025747
1101	82-83	0.3952344973599864
1101	84-85	0.4290783566855225
1101	86-87	0.3836794455798369
1101	88-89	0.6034301210450863
1101	90-91	0.7279403983381947
1101	92-93	0.7573189999392724
1101	94-95	0.7104102341609817
1101	96-97	0.6676694767956839
1101	98-99	0.8080263636457268
1101	100	0.7806636032480263
1102	1	0.19323927967490206
1102	2	0.18526706552449923
1102	3	0.18428645245007047
1102	4	0.2400530274415189
1102	5	0.09146035390903506
1102	6	0.19775893758194485
1102	7	0.1693553657028133
1102	8	0.2211623944521648
1102	9	0.24288781164132445
1102	10-11	0.24932903271465534
1102	12-13	0.18191968695237648
1102	14-15	0.28913286605903465
1102	16-17	0.2573678066040159
1102	18-19	0.2722227474315062
1102	20-21	0.28274420736566697
1102	22-23	0.27170713513555
1102	24-25	0.2711921458909998
1102	26-27	0.38040843676033376
1102	28-29	0.3478014832741323
1102	30-31	0.4140550973361812
1102	32-33	0.3394591333061996
1102	34-35	0.3786446502413128
1102	36-37	0.34754797482649025
1102	38-39	0.41534113287554675
1102	40-41	0.4551721034062197
1102	42-43	0.4760718233932977
1102	44-45	0.44392362835881727
1102	46-47	0.4282522288402575
1102	48-49	0.4440871567086333
1102	50-51	0.4497552047482358
1102	52-53	0.3918051334206609
1102	54-55	0.44767972202607353
1102	56-57	0.4427212807855838
1102	58-59	0.38745876984103944
1102	60-61	0.3354921245376232
1102	62-63	0.36775074568667065
1102	64-65	0.4047931541604939
1102	66-67	0.39343909998458315
1102	68-69	0.3642245997027018
1102	70-71	0.4136703077680721
1102	72-73	0.4368610810224496
1102	74-75	0.4521501894620741
1102	76-77	0.3377830485914046
1102	78-79	0.3647870976383274
1102	80-81	0.4030854310238894
1102	82-83	0.3632384363953918
1102	84-85	0.2500144297702249
1102	86-87	0.34691428267979774
1102	88-89	0.453581539164432
1102	90-91	0.4363235846837661
1102	92-93	0.47553465241622916
1102	94-95	0.45660036337555
1102	96-97	0.42195577219337466
1102	98-99	0.4695053110608818
1102	100	0.48971522692086467
1103	1	-0.019093260966993597
1103	2	0.05774934847583779
1103	3	-0.02028087233484044
1103	4	0.06058294561785971
1103	5	0.012724762590195837
1103	6	0.036329828161562716
1103	7	0.09140272177400988
1103	8	0.07121203642425655
1103	9	0.10545065416435051
1103	10-11	0.08850932129647049
1103	12-13	0.04408223346703011
1103	14-15	0.10265036189147736
1103	16-17	0.03028435805391183
1103	18-19	0.14056934057011716
1103	20-21	0.05559834424056476
1103	22-23	0.14137510013070909
1103	24-25	0.125671379218808
1103	26-27	0.15657739327818376
1103	28-29	0.11557725957186449
1103	30-31	0.09755737138620191
1103	32-33	0.18208288929418615
1103	34-35	0.14053331001341007
1103	36-37	0.20537073323469457
1103	38-39	0.1348524030516529
1103	40-41	0.1282848525707294
1103	42-43	0.14007388807797128
1103	44-45	0.016304915573826406
1103	46-47	0.14806753963587482
1103	48-49	0.16030217360270171
1103	50-51	0.16873147404210442
1103	52-53	0.12112194400953769
1103	54-55	0.2636782630524621
1103	56-57	0.20055259815448068
1103	58-59	0.1520988062890538
1103	60-61	0.2853900753907581
1103	62-63	0.23370082982783202
1103	64-65	0.2145107347924906
1103	66-67	0.2596508249706915
1103	68-69	0.2000442452827258
1103	70-71	0.17860608922751453
1103	72-73	0.23682631847434976
1103	74-75	0.22883005009586554
1103	76-77	0.11695554193516955
1103	78-79	0.1042498319234113
1103	80-81	0.07836794256531476
1103	82-83	0.15419872545125202
1103	84-85	0.18156065210832395
1103	86-87	0.17551990947223572
1103	88-89	0.13860849330684033
1103	90-91	0.17658954451195186
1103	92-93	0.18402435137783613
1103	94-95	0.1091818776005411
1103	96-97	0.1624324141312279
1103	98-99	0.2006471554247362
1103	100	0.3330585939896693
1104	1	0.13008251645035784
1104	2	0.1728308947003825
1104	3	0.18346755080446187
1104	4	0.1549064974089731
1104	5	0.11671572192220481
1104	6	0.22314001418106244
1104	7	0.19364636927806345
1104	8	0.18822135742303203
1104	9	0.27607949619794425
1104	10-11	0.25039935153215254
1104	12-13	0.3197850628056571
1104	14-15	0.252041393308545
1104	16-17	0.2777633833037001
1104	18-19	0.27900857386541134
1104	20-21	0.24484653210223684
1104	22-23	0.2852939243432857
1104	24-25	0.33259116957142254
1104	26-27	0.3738288750266392
1104	28-29	0.36154412547362824
1104	30-31	0.3267231620191424
1104	32-33	0.2933372944923107
1104	34-35	0.3248790880314658
1104	36-37	0.3556106413262299
1104	38-39	0.3315421219199095
1104	40-41	0.3414572710245096
1104	42-43	0.3497590665886108
1104	44-45	0.26938892971051587
1104	46-47	0.2576528503024633
1104	48-49	0.18629809879443116
1104	50-51	0.23751100889322885
1104	52-53	0.14313879889830616
1104	54-55	0.16728877345473592
1104	56-57	0.2703308114461649
1104	58-59	0.1635436862474009
1104	60-61	0.2648205533547525
1104	62-63	0.2270081825039938
1104	64-65	0.17706204533892844
1104	66-67	0.21685945615548974
1104	68-69	0.16082246358031682
1104	70-71	0.29804252422081134
1104	72-73	0.27675092951538005
1104	74-75	0.27510978518326823
1104	76-77	0.2654477627557341
1104	78-79	0.23003801197587848
1104	80-81	0.35926264831810784
1104	82-83	0.3133269968114547
1104	84-85	0.30844762874075116
1104	86-87	0.3650551860986653
1104	88-89	0.4905669292612629
1104	90-91	0.45889366337221205
1104	92-93	0.4654163939881144
1104	94-95	0.4814306311297045
1104	96-97	0.5055032377030422
1104	98-99	0.5835004222796307
1104	100	0.5799724523425276
1105	1	0.07930601049942254
1105	2	0.10254685899477423
1105	3	0.1721235806805872
1105	4	0.1369910705204589
1105	5	0.2504314888641517
1105	6	0.208029269099697
1105	7	0.26078604926349414
1105	8	0.18857241705383387
1105	9	0.27601687475029024
1105	10-11	0.24941638456591164
1105	12-13	0.28475215808133925
1105	14-15	0.2482594373940401
1105	16-17	0.29691510937851007
1105	18-19	0.2607563194932965
1105	20-21	0.2865125662290353
1105	22-23	0.3046951873989059
1105	24-25	0.2783420197050148
1105	26-27	0.30803555616581946
1105	28-29	0.3548645043903207
1105	30-31	0.36318212789235105
1105	32-33	0.375111417032997
1105	34-35	0.35878670431760185
1105	36-37	0.2690146673160356
1105	38-39	0.27359166497311094
1105	40-41	0.2523896474515226
1105	42-43	0.20535970115261648
1105	44-45	0.2078728454865626
1105	46-47	0.25768700745572914
1105	48-49	0.2772585979442965
1105	50-51	0.22440415013684856
1105	52-53	0.2299767194108142
1105	54-55	0.1411149059541259
1105	56-57	0.16224334636236648
1105	58-59	0.2027048624679466
1105	60-61	0.1298684872265028
1105	62-63	0.192559744624468
1105	64-65	0.1834949758708717
1105	66-67	0.25982346210641794
1105	68-69	0.19101074539960194
1105	70-71	0.16095753363300247
1105	72-73	0.10657050384134692
1105	74-75	0.23851513950680214
1105	76-77	0.20490990144895704
1105	78-79	0.17148980484689957
1105	80-81	0.16951207144173708
1105	82-83	0.24785911971648034
1105	84-85	0.26496557263193665
1105	86-87	0.22596915561529585
1105	88-89	0.27494800164406996
1105	90-91	0.2532040304477512
1105	92-93	0.2267252581489103
1105	94-95	0.23215552182681165
1105	96-97	0.32533563993768055
1105	98-99	0.25475964471101165
1105	100	0.4155664831902328
1106	1	0.09083716281542209
1106	2	0.09610060295612399
1106	3	0.027937554820969268
1106	4	0.03303708856535792
1106	5	-0.012544054614103572
1106	6	-0.011530176055181585
1106	7	0.0043375731339097
1106	8	0.0011403518364403453
1106	9	-0.00947660706272302
1106	10-11	-0.017146381845002168
1106	12-13	0.04483070489861518
1106	14-15	0.052898095393473454
1106	16-17	0.06543088978488498
1106	18-19	0.02971123388658725
1106	20-21	0.06014188208580862
1106	22-23	-0.00907292854312658
1106	24-25	0.003011670173897585
1106	26-27	0.009824283432095626
1106	28-29	0.0245167493462759
1106	30-31	-0.0326081356768384
1106	32-33	-0.058445257448390464
1106	34-35	-0.030302658782709102
1106	36-37	-0.0796914636870909
1106	38-39	-0.0295477656179699
1106	40-41	-0.06090447907590146
1106	42-43	-0.07214411740378779
1106	44-45	0.016463043341069294
1106	46-47	0.05123009498058906
1106	48-49	-0.03375749327479838
1106	50-51	-0.019862243014607373
1106	52-53	-0.02854598767926575
1106	54-55	0.031541429824549994
1106	56-57	-0.023219276186651427
1106	58-59	-0.003209015764504386
1106	60-61	-0.05415240826938117
1106	62-63	0.07723867507346682
1106	64-65	0.08585271244376003
1106	66-67	0.10696265292768459
1106	68-69	0.08339339139278223
1106	70-71	0.10654199477252746
1106	72-73	0.12317989216189318
1106	74-75	0.14533858899529406
1106	76-77	0.13193551241798218
1106	78-79	0.14372379355107512
1106	80-81	0.12047736698024636
1106	82-83	0.05133134193870603
1106	84-85	0.038618421452625284
1106	86-87	0.08071704208872887
1106	88-89	0.1939848373928541
1106	90-91	0.20017693645797507
1106	92-93	0.21928684347144412
1106	94-95	0.23784517087483792
1106	96-97	0.14837485562395614
1106	98-99	0.19691006919239484
1106	100	0.30564978100210993
1107	1	0.17021427526997357
1107	2	0.1758359663782727
1107	3	0.21621336541709368
1107	4	0.16393037029349244
1107	5	0.12342658160122966
1107	6	0.14059640595337441
1107	7	0.17305298752152254
1107	8	0.07202126537641362
1107	9	0.11408905628798749
1107	10-11	0.060922741399728864
1107	12-13	0.131970987874233
1107	14-15	0.11164192462717892
1107	16-17	0.1677120741455198
1107	18-19	0.1782473095457746
1107	20-21	0.16945960591269227
1107	22-23	0.15717231343621307
1107	24-25	0.15069023589055774
1107	26-27	0.18338514877876833
1107	28-29	0.19357868624719288
1107	30-31	0.17264434595746536
1107	32-33	0.20663193710534244
1107	34-35	0.16647015039534097
1107	36-37	0.140320478011823
1107	38-39	0.14617166843079588
1107	40-41	0.12869531407681478
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2216	32-33	-0.4828137027018329
2216	34-35	-0.4468144834720391
2216	36-37	-0.46288693882680576
2216	38-39	-0.5214162065498371
2216	40-41	-0.5250437599315063
2216	42-43	-0.5444404113952501
2216	44-45	-0.5014612876361468
2216	46-47	-0.4169761754496477
2216	48-49	-0.4146198805465531
2216	50-51	-0.4798931336217578
2216	52-53	-0.4696295876536851
2216	54-55	-0.4871883127998231
2216	56-57	-0.6823837777450095
2216	58-59	-0.6675430267205584
2216	60-61	-0.5159866914845637
2216	62-63	-0.5285232788309102
2216	64-65	-0.5403187796918374
2216	66-67	-0.4778899727739301
2216	68-69	-0.5994518202173822
2216	70-71	-0.675376282544562
2216	72-73	-0.5144228532473036
2216	74-75	-0.5068978252741942
2216	76-77	-0.5488372022043251
2216	78-79	-0.5043710407857276
2216	80-81	-0.43908026811646295
2216	82-83	-0.36556160231472745
2216	84-85	-0.4645266036384328
2216	86-87	-0.49371505994189135
2216	88-89	-0.40459872368739624
2216	90-91	-0.4027801866187062
2216	92-93	-0.44551506424394205
2216	94-95	-0.5200869907934944
2216	96-97	-0.49342923707898123
2216	98-99	-0.4788140536578389
2216	100	-0.5253378022530022
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	15119.0
3	2235.0
4	1734.0
5	1650.0
6	2625.0
7	5109.0
8	5442.0
9	3909.0
10	3272.0
11	3457.0
12	3465.0
13	3463.0
14	3603.0
15	3536.0
16	3465.0
17	3343.0
18	3277.0
19	3180.0
20	3204.0
21	3539.0
22	4198.0
23	6436.0
24	10469.0
25	12830.0
26	12813.0
27	11293.0
28	12973.0
29	16339.0
30	20828.0
31	27185.0
32	37246.0
33	50640.0
34	74065.0
35	123280.0
36	257803.0
37	526978.0
38	607088.0
39	147405.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.52888983385356	17.59558662126899	12.242312564781757	24.633210980095697
2	16.665496117295923	19.850781967907956	37.61289457541228	25.870827339383844
3	18.98951710759347	24.64957216290403	28.58782343031498	27.77308729918752
4	13.120490110632149	16.40294364748467	35.100532746017166	35.37603349586602
5	14.540085705143682	37.87903325540172	33.112985134103226	14.46789590535137
6	33.08155958432802	35.13367262936765	17.02735267654334	14.757415109760991
7	29.868170726562187	31.43857370066827	20.520811972562715	18.17244360020683
8	26.829085207179553	34.06172604492521	19.288530451881826	19.82065829601341
9	26.60316575621656	15.733058710049033	18.637749449755812	39.0260260839786
10-11	25.58467355649765	25.537645026101202	26.776897093381685	22.10078432401946
12-13	26.194020367781874	23.09675751231835	26.798101676022057	23.911120443877717
14-15	23.658315979168623	24.89964676741527	23.482374309768712	27.959662943647395
16-17	23.91192033971901	27.47452966040365	24.31213523995967	24.30141475991767
18-19	24.049467178599123	27.122828218301855	25.258670874384464	23.569033728714558
20-21	25.16551807211772	25.89122898349198	25.137893905044013	23.805359039346282
22-23	24.802370693091653	25.543751970160272	25.144507039834956	24.50937029691312
24-25	24.64867670946341	25.74364789385119	24.663813768541367	24.943861628144038
26-27	24.48862989610196	25.800480042705352	25.211907649674803	24.498982411517886
28-29	24.05339321736771	26.19296851598855	24.989776325379516	24.763861941264224
30-31	24.093679324503242	25.753890364495746	25.557535297423712	24.5948950135773
32-33	24.836253031692504	25.971233329064553	24.639717416573564	24.552796222669382
34-35	24.54208087165312	26.049335652979384	24.728368859046178	24.68021461632132
36-37	24.488703597226774	26.00701646627563	25.31285138749615	24.191428549001444
38-39	24.384558794489553	26.36719262650474	24.751266206424212	24.496982372581503
40-41	23.94361429457648	26.085465548541997	25.371531483621464	24.599388673260062
42-43	24.34823050137021	25.735880657818438	25.115484204566975	24.80040463624438
44-45	24.417117478209207	25.95838028690698	24.630769525123373	24.99373270976044
46-47	24.563744560604796	25.79617537542252	25.124707486864505	24.51537257710818
48-49	24.595697314819905	25.9367420704379	24.693860230920777	24.773700383821417
50-51	23.728251055298912	26.262857182103545	25.312260305675494	24.69663145692205
52-53	24.306807742922988	25.952602008214864	25.12449239652616	24.61609785233599
54-55	24.0602569722039	26.17245438305552	24.67819606817762	25.089092576562955
56-57	24.248116981165396	25.84025760345198	25.065391796444608	24.84623361893801
58-59	24.4613753685959	25.206931246287063	25.1666561195872	25.16503726552984
60-61	24.47011573419987	25.69927651408119	25.23652612947788	24.59408162224106
62-63	23.86308615394125	25.519075742256845	25.372029832046344	25.24580827175556
64-65	24.00569244463369	25.78676437944431	25.1160968785006	25.091446297421406
66-67	24.1301954766829	25.723226444490667	24.914152731611026	25.232425347215408
68-69	24.18090930497233	25.545504493544207	25.334930928927434	24.938655272556023
70-71	24.324604075713903	25.2093768449629	25.388430992654122	25.077588086669074
72-73	24.07350039306007	25.276423631678803	25.410763100673663	25.239312874587473
74-75	23.9230353954669	25.594120762101262	25.35369835253499	25.129145489896835
76-77	24.229546115138533	24.810843493180506	25.329274708940464	25.6303356827405
78-79	24.118895403598003	25.29208266616433	25.459193785124484	25.12982814511318
80-81	23.826275418598673	25.535205850668248	25.475675850687868	25.16284288004521
82-83	23.785158148046676	25.26530112674336	25.635698659617645	25.313842065592322
84-85	23.99049386010698	25.18067454446272	25.638223139685017	25.190608455745284
86-87	24.583520461636837	25.471311912692045	25.215337563107514	24.729830062563604
88-89	23.53163981928634	25.549974226416182	25.34287256153397	25.57551339276351
90-91	23.937387451973134	25.09978014189473	25.578537268651107	25.384295137481033
92-93	24.3770061004455	25.22772717996351	25.72822973072015	24.667036988870837
94-95	23.654001361499947	25.103093279524725	25.597679479027647	25.64522587994768
96-97	23.862974944256283	25.121227321614548	25.85025671595537	25.165541018173798
98-99	23.75999224281949	25.38048797698703	25.518409856948608	25.34110992324487
100	23.759486799901307	25.122871946706145	25.4420429311621	25.675598322230446
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2507.0
1	1695.5
2	828.5
3	736.5
4	581.0
5	408.5
6	356.5
7	375.0
8	440.0
9	490.0
10	408.5
11	314.0
12	291.5
13	282.5
14	284.5
15	281.5
16	306.5
17	354.5
18	456.0
19	559.5
20	656.0
21	792.5
22	974.5
23	1231.5
24	1611.0
25	2308.5
26	3387.0
27	4855.0
28	6553.5
29	7837.5
30	9179.0
31	11368.0
32	13632.0
33	16504.5
34	20610.0
35	23975.5
36	26364.0
37	28657.0
38	30483.0
39	35897.0
40	44949.0
41	52389.0
42	57309.5
43	62500.0
44	71506.5
45	77263.5
46	81706.0
47	88410.5
48	97881.5
49	99961.5
50	94932.5
51	95337.0
52	87705.0
53	81534.0
54	80502.5
55	75043.5
56	69682.0
57	62882.0
58	55721.0
59	51540.5
60	47264.0
61	42258.0
62	40111.0
63	36122.0
64	29104.5
65	23591.0
66	18805.5
67	14084.0
68	10665.5
69	8595.0
70	6092.5
71	3839.5
72	2915.0
73	2358.0
74	2102.0
75	1528.0
76	927.5
77	679.0
78	442.5
79	225.0
80	124.5
81	84.0
82	78.0
83	58.5
84	36.0
85	21.5
86	18.5
87	17.0
88	12.0
89	12.0
90	11.0
91	4.0
92	1.5
93	2.0
94	2.0
95	1.5
96	1.5
97	1.5
98	1.5
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32969625855329027
2	0.12077253214673421
3	0.06470307469599612
4	0.03919466010773381
5	0.04213793871407177
6	0.010056201904988023
7	0.007309141872405929
8	0.015255994109518415
9	0.01751250770771085
10-11	0.020529368279207257
12-13	0.015893704474224973
14-15	0.01802758146381999
16-17	0.019033201654318793
18-19	0.01881245575884345
20-21	0.02317831902491142
22-23	0.015722013222188594
24-25	0.024453739754324536
26-27	0.019180365584635692
28-29	0.018714346471965515
30-31	0.01954827541042794
32-33	0.024036775285093324
34-35	0.014765447675128756
36-37	0.009565655470598363
38-39	0.013220226406801327
40-41	0.00929585493168405
42-43	0.008290234741185249
44-45	0.008927945105891807
46-47	0.008486453314941113
48-49	0.004660191126701767
50-51	0.006843122759735753
52-53	0.008118543489148868
54-55	0.003605516292763999
56-57	0.002992333249776924
58-59	0.003139497180093822
60-61	0.003875316831678311
62-63	0.003139497180093822
64-65	0.0025263141371067474
66-67	0.0019131310941196728
68-69	0.0030904425366548556
70-71	0.0026244234239846793
72-73	0.0024772594936677815
74-75	0.0023300955633508836
76-77	0.00419417201403159
78-79	0.003875316831678311
80-81	0.0036790982579224477
82-83	0.002820641997740543
84-85	0.003482879684166584
86-87	0.002820641997740543
88-89	0.6006005269449799
90-91	0.6113925485015523
92-93	0.6072719584526792
94-95	0.5936347675766467
96-97	0.5926536747078673
98-99	0.590299051822797
100	0.5907650709354672
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2038543.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.486568462186938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.73423976584841	11.22767681430233
2	10.60256935919196	3.920102486675753
3	5.308893875495036	2.944296902634717
4	3.6030397413007895	2.6643136339814943
5	2.6086701882381464	2.411268001506529
6	2.044579224334422	2.2678352284214007
7	1.5946512402229658	2.0635740527985216
8	1.3333892698312122	1.9719833618784124
9	1.1332297546523133	1.8854576498490565
>10	9.58056874168	35.92408013854775
>50	0.9936645041074851	12.556334938983305
>100	0.4270092150892507	13.999397244894835
>500	0.026154298953404306	3.3339028384644775
>1k	0.009340821054787252	2.8297767070614226
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	4849	0.237865966035546	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3631	0.17811741032688544	No Hit
GTACACAGGAGGCAAAGTGTTTCACATCATAGACTTCACTTCCAACTCCT	3054	0.14981288106260204	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	2611	0.12808167401914014	No Hit
GTACAAGAGTTACAGCCCTTATGACATGTTGGAAAGCATCAGGAAAGAGG	2574	0.1262666522118984	No Hit
CTCTTGTACCTATCAAATACTTTCTGGAGGTGGGGCACGCTCCGCTCGGT	2446	0.11998765785171076	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.810928687793194E-5	0.0
10-11	0.0	0.0	0.0	9.810928687793194E-5	0.0
12-13	0.0	0.0	0.0	9.810928687793194E-5	0.0
14-15	0.0	0.0	0.0	9.810928687793194E-5	0.0
16-17	0.0	0.0	0.0	1.2263660859741492E-4	0.0
18-19	0.0	0.0	0.0	5.396010778286256E-4	0.0
20-21	0.0	0.0	0.0	7.848742950234554E-4	0.0
22-23	0.0	0.0	0.0	0.0010546748339377683	0.0
24-25	0.0	0.0	0.0	0.0018640764506807068	0.0
26-27	0.0	0.0	0.0	0.004267753979190039	0.0
28-29	0.0	0.0	0.0	0.009443018862000949	0.0
30-31	0.0	0.0	0.0	0.026170652274688344	0.0
32-33	0.0	0.0	0.0	0.05702602299779794	0.0
34-35	0.0	0.0	0.0	0.10411848069920526	0.0
36-37	0.0	0.0	0.0	0.1781664649703244	0.0
38-39	0.0	0.0	0.0	0.2893488143247408	0.0
40-41	0.0	0.0	0.0	0.4163758135099431	0.0
42-43	0.0	0.0	0.0	0.5321938266693418	0.0
44-45	0.0	0.0	0.0	0.6671186234482177	0.0
46-47	0.0	0.0	0.0	0.8326044630895694	0.0
48-49	0.0	0.0	0.0	1.006920138549935	0.0
50-51	0.0	0.0	0.0	1.1859695871021607	0.0
52-53	0.0	0.0	0.0	1.3596475521978197	0.0
54-55	0.0	0.0	0.0	1.5452703229708669	0.0
56-57	0.0	0.0	0.0	1.7673161664973465	0.0
58-59	0.0	0.0	0.0	2.0048632773505393	0.0
60-61	0.0	0.0	0.0	2.260609660919588	0.0
62-63	0.0	0.0	0.0	2.5162088805583203	0.0
64-65	0.0	0.0	0.0	2.7810549004852976	0.0
66-67	0.0	0.0	0.0	3.086272891962544	0.0
68-69	0.0	0.0	0.0	3.4284780845927703	0.0
70-71	0.0	0.0	0.0	3.7942540333954202	0.0
72-73	0.0	0.0	0.0	4.162727987587213	0.0
74-75	0.0	0.0	0.0	4.54375993049938	0.0
76-77	0.0	0.0	0.0	4.953586949110223	0.0
78-79	0.0	0.0	0.0	5.410751698639666	0.0
80-81	0.0	0.0	0.0	5.898992564787694	0.0
82-83	0.0	4.905464343896597E-5	0.0	6.414949304478738	0.0
84-85	0.0	4.905464343896597E-5	0.0	6.9320097736471595	0.0
86-87	0.0	4.905464343896597E-5	0.0	7.471022195754516	0.0
88	0.0	4.905464343896597E-5	0.0	7.905793500554072	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATG	55	0.0027275546	34.156075	9
GTACATG	9285	0.0	28.252731	1
TAGCGAA	35	0.008878573	26.832312	42-43
GTTCTAA	655	0.0	26.604042	1
TACATGG	9555	0.0	26.517578	2
GTATATA	385	0.0	25.688913	1
GTATAGA	360	0.0	24.856401	1
GTACACA	1300	0.0	24.272728	1
ACATGGG	10155	0.0	23.507133	3
TATTAGG	430	0.0	22.95761	2
CATGGGG	6925	0.0	22.321737	4
TGACACA	960	0.0	21.524916	6
ATGGGGC	3105	0.0	21.336348	5
ACCTATC	1500	0.0	21.290623	8
GTACCTA	1440	0.0	20.872646	6
GTATTAG	660	0.0	20.693848	1
GACTGCG	185	7.233053E-6	20.30852	7
GACCGTG	650	0.0	20.23041	7
TAGACGA	70	5.247131E-4	20.12374	52-53
GGACCGA	380	0.0	19.774086	6
>>END_MODULE