FastQCFastQC Report
Wed 25 May 2016
SRR1033178_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033178_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2038543
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52030.2552313098129399No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49450.2425752118056867No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT47390.2324699552572597No Hit
GTACACAGGAGGCAAAGTGTTTCACATCATAGACTTCACTTCCAACTCCT30910.15162790286984382No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27120.1330361930064757No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26340.12920993081823637No Hit
GTACAAGAGTTACAGCCCTTATGACATGTTGGAAAGCATCAGGAAAGAGG25780.12646287078565424No Hit
CTCTTGTACCTATCAAATACTTTCTGGAGGTGGGGCACGCTCCGCTCGGT24390.11964427534763798No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCAC350.00884457826.85342878-79
GTACATG88150.026.7962171
GTACACA14900.026.2110181
CACTACG909.5251744E-426.108787
TACATGG90350.025.7031142
ATTACGG1701.2420787E-724.888075
ATCCGAT1151.3785534E-424.5195527
TTCGACG500.00176086123.49675270-71
ACATGGG97600.022.8786683
GTAATCG2704.7293724E-1122.62650194
TACACTG11250.022.5651825
TGACACA10250.022.4662886
CATGGGG66350.022.3889184
CCTAATA1954.6685636E-721.6967582
GTATACA2005.9027843E-721.1740821
GTATAGC3650.020.6261981
TATCCCG1150.003954407820.4394765
GAGGCAA16500.020.2219439
GTACCTA12550.020.2213046
ATAGGGG2109.516498E-720.146993
Produced by FastQC (version 0.11.5)