##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033178_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2038543
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74677649674302	34.0	31.0	34.0	31.0	34.0
2	32.822228915455796	34.0	31.0	34.0	31.0	34.0
3	32.91197830999886	34.0	31.0	34.0	31.0	34.0
4	36.35360402012614	37.0	37.0	37.0	35.0	37.0
5	36.28994973370687	37.0	37.0	37.0	35.0	37.0
6	36.36811045928391	37.0	37.0	37.0	35.0	37.0
7	36.340632991308006	37.0	37.0	37.0	35.0	37.0
8	36.32911005556419	37.0	37.0	37.0	35.0	37.0
9	38.154035014223396	39.0	39.0	39.0	37.0	39.0
10-11	38.13170828380858	39.0	39.0	39.0	37.0	39.0
12-13	38.09660747896905	39.0	39.0	39.0	37.0	39.0
14-15	39.61667205450167	41.0	40.0	41.0	37.0	41.0
16-17	39.62480531438385	41.0	40.0	41.0	37.0	41.0
18-19	39.626591393951465	41.0	40.0	41.0	37.0	41.0
20-21	39.61556587229212	41.0	40.0	41.0	37.0	41.0
22-23	39.54405474890645	41.0	40.0	41.0	37.0	41.0
24-25	39.480064438179625	41.0	39.0	41.0	36.0	41.0
26-27	39.373125070209454	41.0	39.0	41.0	36.0	41.0
28-29	39.28067742500403	41.0	39.0	41.0	36.0	41.0
30-31	39.17565535777268	41.0	39.0	41.0	35.5	41.0
32-33	39.20203253009625	41.0	39.0	41.0	35.0	41.0
34-35	39.124824936241225	41.0	39.0	41.0	35.0	41.0
36-37	39.02733839806176	41.0	39.0	41.0	35.0	41.0
38-39	38.924441623257394	41.0	39.0	41.0	35.0	41.0
40-41	38.8031133020005	41.0	39.0	41.0	35.0	41.0
42-43	38.66289256591595	41.0	38.0	41.0	35.0	41.0
44-45	38.47279601166127	41.0	38.0	41.0	35.0	41.0
46-47	38.205984862718125	40.0	38.0	41.0	34.0	41.0
48-49	37.951268381388076	40.0	37.5	41.0	34.0	41.0
50-51	37.747871151111354	40.0	37.0	41.0	34.0	41.0
52-53	37.56749379336124	40.0	37.0	41.0	33.0	41.0
54-55	37.35201072530724	40.0	36.0	41.0	33.0	41.0
56-57	37.1238774458032	39.5	35.5	41.0	33.0	41.0
58-59	36.861956554264495	39.0	35.0	41.0	33.0	41.0
60-61	36.59012588893145	39.0	35.0	41.0	32.0	41.0
62-63	36.29866453638702	38.0	35.0	41.0	32.0	41.0
64-65	35.99064331731045	37.0	35.0	40.0	32.0	41.0
66-67	35.643292047310254	37.0	35.0	40.0	31.5	41.0
68-69	35.27653083599414	36.0	35.0	39.0	31.0	41.0
70-71	34.9033832006487	35.5	35.0	39.0	31.0	41.0
72-73	34.5415949038112	35.0	35.0	37.5	31.0	40.0
74-75	34.235003137044444	35.0	35.0	37.0	30.0	39.5
76-77	33.94172749851242	35.0	34.0	37.0	30.0	39.0
78-79	33.65518142124056	35.0	34.0	36.0	30.0	39.0
80-81	33.407052487977936	35.0	34.0	36.0	30.0	37.0
82-83	33.17063412447027	35.0	34.0	35.0	29.5	37.0
84-85	32.9913300823186	35.0	34.0	35.0	29.0	36.5
86-87	32.84175879537493	35.0	34.0	35.0	29.0	36.0
88-89	32.69128195971338	35.0	34.0	35.0	29.0	36.0
90-91	32.56237199803978	35.0	34.0	35.0	29.0	36.0
92-93	32.433314136616204	35.0	34.0	35.0	29.0	35.0
94-95	32.328394593589636	35.0	34.0	35.0	29.0	35.0
96-97	32.24753831535563	35.0	34.0	35.0	29.0	35.0
98-99	32.173986764076105	35.0	34.0	35.0	29.0	35.0
100	32.082835142550344	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1709597127123601
1101	2	0.1645794284478228
1101	3	0.17918970480884866
1101	4	0.10433603514496781
1101	5	0.07666564538715193
1101	6	0.09862763804701302
1101	7	0.1628807957985714
1101	8	0.13181648422850856
1101	9	0.15189332367925346
1101	10-11	0.1735110529823416
1101	12-13	0.1600476818337171
1101	14-15	0.1710271305954194
1101	16-17	0.21065587091270288
1101	18-19	0.24762293002063274
1101	20-21	0.2267350068253151
1101	22-23	0.26414847765621374
1101	24-25	0.1883649128843743
1101	26-27	0.18013799985784829
1101	28-29	0.2042761368707886
1101	30-31	0.17216251765945856
1101	32-33	0.18410450417300694
1101	34-35	0.1936368993253197
1101	36-37	0.22440369463090803
1101	38-39	0.17821286968052164
1101	40-41	0.17221383032475046
1101	42-43	0.15060429207507298
1101	44-45	0.1941611302207491
1101	46-47	0.2878281673280938
1101	48-49	0.15418156871458422
1101	50-51	0.15844448875472494
1101	52-53	0.2521542741563749
1101	54-55	0.2004479044316625
1101	56-57	0.2271762034079572
1101	58-59	0.24760800859722565
1101	60-61	0.27840578958065265
1101	62-63	0.2867552635542765
1101	64-65	0.28372993051982576
1101	66-67	0.32277796884772414
1101	68-69	0.3767711887246108
1101	70-71	0.2845704191423337
1101	72-73	0.3409004340813695
1101	74-75	0.2786643909211648
1101	76-77	0.3211637900393782
1101	78-79	0.3745585876903732
1101	80-81	0.2596372943523022
1101	82-83	0.2967666878911004
1101	84-85	0.3540784840672373
1101	86-87	0.22788644567000915
1101	88-89	0.2720772257073065
1101	90-91	0.3697785816889265
1101	92-93	0.3230445807396549
1101	94-95	0.33969602432564727
1101	96-97	0.30193441402831667
1101	98-99	0.2754433768904505
1101	100	0.37581937485047945
1102	1	0.0935877452368743
1102	2	0.09518625522127877
1102	3	0.09636035860659575
1102	4	0.07003290811246643
1102	5	0.06535853825293714
1102	6	0.07535386565199786
1102	7	0.09001474520044894
1102	8	0.15135350050630336
1102	9	0.12187940553216237
1102	10-11	0.12258342623730556
1102	12-13	0.09562616730845264
1102	14-15	0.17029889740096849
1102	16-17	0.15853218808663172
1102	18-19	0.12153911258588579
1102	20-21	0.12409699629901638
1102	22-23	0.12806418296442956
1102	24-25	0.17700161705826645
1102	26-27	0.2580650561142406
1102	28-29	0.20382038414764736
1102	30-31	0.18841381346781816
1102	32-33	0.19196731386287524
1102	34-35	0.2609540044395189
1102	36-37	0.23618098318939218
1102	38-39	0.20512211980982187
1102	40-41	0.26876722983772083
1102	42-43	0.31388377490602437
1102	44-45	0.3163701477149985
1102	46-47	0.35046662736392165
1102	48-49	0.24330987538441917
1102	50-51	0.2303085770494988
1102	52-53	0.2985605841603487
1102	54-55	0.22170138119367522
1102	56-57	0.2524168574942678
1102	58-59	0.20871461228075816
1102	60-61	0.21328892265101018
1102	62-63	0.21721377992863466
1102	64-65	0.28880272886652136
1102	66-67	0.25691594274731244
1102	68-69	0.2859580537230286
1102	70-71	0.29843362617643976
1102	72-73	0.29765165108681657
1102	74-75	0.277234423396834
1102	76-77	0.19743212836355895
1102	78-79	0.22507752147594573
1102	80-81	0.21392203588987257
1102	82-83	0.16746762864241305
1102	84-85	0.22252327541711736
1102	86-87	0.1549506101039242
1102	88-89	0.2425147594205157
1102	90-91	0.19120814409971132
1102	92-93	0.2265142116534875
1102	94-95	0.23913491433676626
1102	96-97	0.20554514099067234
1102	98-99	0.20175058946517055
1102	100	0.12278907013850926
1103	1	0.06570870837013842
1103	2	0.07290003830824077
1103	3	0.06724777798776671
1103	4	0.014028072958048199
1103	5	-0.012332069801963996
1103	6	0.010514339581405352
1103	7	0.07701197209717492
1103	8	0.08134436216979424
1103	9	0.07502080746358786
1103	10-11	0.10455944007912166
1103	12-13	0.10606888942665194
1103	14-15	0.0763527161458768
1103	16-17	0.11276255317239503
1103	18-19	0.11981889316046335
1103	20-21	0.11217502241833444
1103	22-23	0.08694298544731538
1103	24-25	0.11910604164592087
1103	26-27	0.16964045792452964
1103	28-29	0.12742270586448967
1103	30-31	0.2188374951903569
1103	32-33	0.17594875803607124
1103	34-35	0.14400344363007633
1103	36-37	0.11446571479355327
1103	38-39	0.06361958767421783
1103	40-41	0.15811040202558502
1103	42-43	0.12333721284302612
1103	44-45	0.1860710317898011
1103	46-47	0.10455425532038021
1103	48-49	0.12157103692756266
1103	50-51	0.11608371603384882
1103	52-53	0.12983107628741664
1103	54-55	0.12417636927697373
1103	56-57	0.14714190417352313
1103	58-59	0.1824126626811875
1103	60-61	0.1728200496159431
1103	62-63	0.171941486469521
1103	64-65	0.15271064498343634
1103	66-67	0.10536900935709781
1103	68-69	0.1634104593176886
1103	70-71	0.19919050165540853
1103	72-73	0.2174252204894529
1103	74-75	0.2030191484171766
1103	76-77	0.058859921020761874
1103	78-79	0.030322320919751178
1103	80-81	-0.010313517242842352
1103	82-83	0.05322222612107197
1103	84-85	0.05372371574272705
1103	86-87	0.038835535535625354
1103	88-89	0.06634922648694896
1103	90-91	-0.003997257789578157
1103	92-93	-0.06414085169598849
1103	94-95	-0.14193859001777298
1103	96-97	-0.11965217597265365
1103	98-99	-0.0791290873484769
1103	100	-0.10716176192415361
1104	1	-0.0016159847677670314
1104	2	0.0781011756503105
1104	3	0.08848458742290433
1104	4	0.03857754133072433
1104	5	0.04451425886586691
1104	6	0.009875085261306538
1104	7	0.07631815339110659
1104	8	0.06001569434175735
1104	9	0.09701476032797274
1104	10-11	0.09948870661362719
1104	12-13	0.10437163666839666
1104	14-15	0.09416823846221689
1104	16-17	0.19057800040011585
1104	18-19	0.1886936653917033
1104	20-21	0.13088897988815518
1104	22-23	0.16365100090555273
1104	24-25	0.151122195707714
1104	26-27	0.0788214164555967
1104	28-29	0.14730629349790547
1104	30-31	0.13234380763903886
1104	32-33	0.06334613694318136
1104	34-35	0.08548739469232913
1104	36-37	0.12465886771400392
1104	38-39	0.16443204637548803
1104	40-41	0.11214741570171327
1104	42-43	0.10464465562550629
1104	44-45	0.09729715361355318
1104	46-47	0.17021911485463193
1104	48-49	0.16836587984220586
1104	50-51	0.18216218863151568
1104	52-53	0.26770464209507594
1104	54-55	0.1449501323173621
1104	56-57	0.2356990265779615
1104	58-59	0.2576419332204267
1104	60-61	0.2205034410178044
1104	62-63	0.26925444075558147
1104	64-65	0.2478585445048722
1104	66-67	0.2838996755431964
1104	68-69	0.18509179329178238
1104	70-71	0.13182100565012433
1104	72-73	0.15681557610908925
1104	74-75	0.11981571836972194
1104	76-77	0.08630856041524737
1104	78-79	0.15832023345654278
1104	80-81	0.1868022043085844
1104	82-83	0.2482537383054506
1104	84-85	0.18365841368630242
1104	86-87	0.183548672036693
1104	88-89	0.17013932380178431
1104	90-91	0.17375786825916606
1104	92-93	0.18756866553433582
1104	94-95	0.21470024380614205
1104	96-97	0.21722834303977123
1104	98-99	0.31826846676537457
1104	100	0.2720254289610935
1105	1	0.07910306126089495
1105	2	0.08728565463986371
1105	3	0.11810073692496559
1105	4	0.059331807179646034
1105	5	0.08410050188262375
1105	6	0.06656077872742827
1105	7	0.03096314671148548
1105	8	0.042047134103725625
1105	9	0.07655652314070238
1105	10-11	0.09487654211277885
1105	12-13	0.0887428414291449
1105	14-15	0.14523602902573884
1105	16-17	0.1367690982869263
1105	18-19	0.08023142043177955
1105	20-21	0.08678165811258509
1105	22-23	0.14265194212489263
1105	24-25	0.10863544109270151
1105	26-27	0.06548779215391676
1105	28-29	0.0898188557398214
1105	30-31	0.18216391329850268
1105	32-33	0.18685935196625536
1105	34-35	0.2316629320982102
1105	36-37	0.17811671019639164
1105	38-39	0.15935686268590388
1105	40-41	0.2735295279800738
1105	42-43	0.21354145547977055
1105	44-45	0.28064611068180767
1105	46-47	0.3961867111019046
1105	48-49	0.31329468115383463
1105	50-51	0.28937390225794246
1105	52-53	0.2666827739265045
1105	54-55	0.33356877908685334
1105	56-57	0.25887465506958307
1105	58-59	0.2435416705898703
1105	60-61	0.19586759422401911
1105	62-63	0.1715488526453086
1105	64-65	0.14692036136180064
1105	66-67	0.11469177992746182
1105	68-69	0.06740521933016197
1105	70-71	0.21831450820052822
1105	72-73	0.20421811436509785
1105	74-75	0.16535953820212512
1105	76-77	0.02948908476550116
1105	78-79	0.11044518791366897
1105	80-81	0.14406401509314293
1105	82-83	0.11714435191973394
1105	84-85	0.13639060642373124
1105	86-87	0.1694199451117555
1105	88-89	0.12860707185862452
1105	90-91	0.11781699049623029
1105	92-93	0.16440916680954132
1105	94-95	0.19638441917656024
1105	96-97	0.22721077108203502
1105	98-99	0.19532548643989855
1105	100	0.24063120986951958
1106	1	0.09907366725691702
1106	2	0.12099196325026185
1106	3	0.10254189563482896
1106	4	0.019899884048072636
1106	5	-0.003287161516070114
1106	6	0.042150434879744125
1106	7	0.01730292718889359
1106	8	0.08120731810485893
1106	9	0.08688039000628578
1106	10-11	0.07522673925685552
1106	12-13	0.08748360585085635
1106	14-15	0.11067698348722388
1106	16-17	0.08079438956286111
1106	18-19	0.101939913611659
1106	20-21	0.07220032286109301
1106	22-23	0.12797714104504365
1106	24-25	0.06595929907792453
1106	26-27	0.02874861731731926
1106	28-29	0.0552162964189975
1106	30-31	0.14779068937124862
1106	32-33	0.11153277658972627
1106	34-35	0.05143017776459402
1106	36-37	0.038516948190135736
1106	38-39	0.09167672450744391
1106	40-41	0.03724280767600874
1106	42-43	0.057635125556494415
1106	44-45	0.11569890444424402
1106	46-47	0.06965018616584473
1106	48-49	0.12637812105153756
1106	50-51	-0.048263743380019264
1106	52-53	-0.12725304768474643
1106	54-55	-0.07740490839538694
1106	56-57	-0.06646235473434103
1106	58-59	-0.13596178387162183
1106	60-61	-0.046144740601214096
1106	62-63	-0.07181773765022825
1106	64-65	-0.09824339254555525
1106	66-67	-0.038743366776152754
1106	68-69	-0.03180354026063981
1106	70-71	0.010533533491802416
1106	72-73	0.10159663098914251
1106	74-75	0.0051011640191234164
1106	76-77	-0.011306640227395803
1106	78-79	0.027523459439670717
1106	80-81	0.09357115490558243
1106	82-83	0.10945773107574297
1106	84-85	0.15331258354791544
1106	86-87	0.14261478917256198
1106	88-89	0.1879125830123627
1106	90-91	0.1480584031844856
1106	92-93	0.20878767455321423
1106	94-95	0.12466482554200553
1106	96-97	0.17563561656370297
1106	98-99	0.26974293457857357
1106	100	0.3360809114892973
1107	1	-0.012272546051683264
1107	2	0.020902400508362007
1107	3	0.010822184368045384
1107	4	0.007534361438814585
1107	5	-0.004095358639396807
1107	6	-0.07207671329328491
1107	7	-0.07172243825907998
1107	8	-0.013768105452271584
1107	9	-0.003258645488280365
1107	10-11	-0.015008522874104813
1107	12-13	0.004130014278281635
1107	14-15	0.07683772728447025
1107	16-17	0.07340999480441468
1107	18-19	0.0015448779464932727
1107	20-21	0.00890185502370855
1107	22-23	0.03794925931050841
1107	24-25	-0.015877323553723954
1107	26-27	4.8091001688987944E-4
1107	28-29	-0.02465474389489941
1107	30-31	0.035146615972450945
1107	32-33	0.005114913087609807
1107	34-35	-0.0035440196359530773
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2216	10-11	-0.0997136230292952
2216	12-13	-0.02852721882636189
2216	14-15	-0.14931708731397464
2216	16-17	-0.13116094092514885
2216	18-19	-0.11443012025218735
2216	20-21	-0.05742510043702964
2216	22-23	-0.0748509398478987
2216	24-25	-0.16413263807602618
2216	26-27	-0.18697827998308014
2216	28-29	-0.19689777410562215
2216	30-31	-0.1420635958145695
2216	32-33	-0.10426261765013578
2216	34-35	-0.12540424374093817
2216	36-37	-0.1563122459401285
2216	38-39	-0.12126522120321681
2216	40-41	-0.21929136819128558
2216	42-43	-0.13875977464108757
2216	44-45	0.06165395489428249
2216	46-47	-0.07519555959081003
2216	48-49	-0.1493005820615494
2216	50-51	-0.16837375335421711
2216	52-53	-0.08881705969526621
2216	54-55	-0.08734141583799726
2216	56-57	-0.04688316362917533
2216	58-59	-0.058194637164575624
2216	60-61	-0.08114663345039475
2216	62-63	-0.08649699911061504
2216	64-65	-0.12183222594543963
2216	66-67	-0.08467430284625266
2216	68-69	-0.061778492420820896
2216	70-71	-0.10012156518700266
2216	72-73	-0.055487162591767
2216	74-75	-0.023182886090388877
2216	76-77	-0.07297497502141681
2216	78-79	-0.05526833752243476
2216	80-81	-0.05699760737848436
2216	82-83	-0.09557789597592148
2216	84-85	-0.11069804406555761
2216	86-87	-0.2163904624035098
2216	88-89	-0.16758700816679095
2216	90-91	-0.15171713428960487
2216	92-93	-0.09975279179641916
2216	94-95	-0.15673019565963386
2216	96-97	-0.15801120370896626
2216	98-99	-0.21215096906416875
2216	100	-0.25804710734584546
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3.0
3	6.0
4	21.0
5	40.0
6	86.0
7	192.0
8	370.0
9	573.0
10	951.0
11	1465.0
12	2266.0
13	3538.0
14	3731.0
15	4785.0
16	6415.0
17	10092.0
18	11937.0
19	6506.0
20	4223.0
21	4231.0
22	4214.0
23	4718.0
24	5513.0
25	6526.0
26	8344.0
27	10595.0
28	12722.0
29	16342.0
30	20896.0
31	26734.0
32	34339.0
33	45612.0
34	65035.0
35	106450.0
36	232122.0
37	523803.0
38	669810.0
39	183242.0
40	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.49595174000798	17.453055060361812	12.488554355580147	24.562438844050057
2	16.969557831388542	19.768106386934452	37.357329161681434	25.905006619995575
3	19.06621542935322	24.30549662185198	29.252804576602013	27.37548337219279
4	13.32452965135359	16.151257198162806	35.2013523397371	35.32286081074651
5	14.257132565760742	37.25967554665256	33.66043991312054	14.822751974466156
6	32.979207688632364	35.036707607692165	16.863277774016918	15.120806929658551
7	29.585180526605225	31.165741864050826	20.880491235265175	18.368586374078774
8	27.343486713239372	33.034687199078945	19.458551811523286	20.163274276158393
9	27.090943964335278	14.310430842450556	19.101210130186246	39.497415063027915
10-11	25.932728193173926	24.505576551015043	27.201849758846546	22.35984549696449
12-13	26.820994210080435	22.17301768959497	27.375556954157943	23.63043114616665
14-15	23.97234587426237	23.830062566883086	24.583813354518448	27.613778204336096
16-17	23.640536402715075	26.51511398091676	25.37290113576216	24.471448480606
18-19	23.6999532018472	25.650906383091442	27.235224979421567	23.413915435639783
20-21	24.720498905345632	24.386583947456593	27.384092462116328	23.508824685081454
22-23	24.263748953293465	24.18028223181625	27.49864240874706	24.05732640614323
24-25	24.232061820623848	24.41128296042811	26.96644122787697	24.390213991071075
26-27	24.06658361619793	24.432976971767992	27.521419343793376	23.9790200682407
28-29	23.64574009620906	24.72184317545337	27.291440945440076	24.34097578289749
30-31	23.720510958952172	24.23529255891429	27.87115076174477	24.17304572038877
32-33	24.438096878317353	24.46554957026356	26.91323060285768	24.183122948561408
34-35	24.086359705346574	24.547280098626707	26.992829936395584	24.37353025963113
36-37	24.045920353789075	24.53445093730504	27.560430911795365	23.859197797110525
38-39	24.01800537128841	24.87799049929136	26.97096280602348	24.133041323396753
40-41	23.674297952082696	24.63884636927773	27.440070247113702	24.24678543152587
42-43	24.209951219320722	24.270154290609597	27.053749027281267	24.46614546278842
44-45	24.245808219366275	24.618650814191767	26.426414860125146	24.70912610631681
46-47	24.534250853122177	24.56660706484023	26.604999341346257	24.29414274069133
48-49	24.665731916359142	24.881962426177456	25.872109281403343	24.580196376060055
50-51	23.758328219030506	25.256168928488552	26.38097214434255	24.604530708138387
52-53	24.332610969868	25.059217860651305	26.203961593320408	24.404209576160287
54-55	24.054592811728973	25.30800621151072	25.729228268113367	24.908172708646937
56-57	24.211594182280912	24.927317756003013	26.132323504111977	24.728764557604098
58-59	24.39242864697335	24.347984769387292	26.286174422106956	24.9734121615324
60-61	24.36465875786504	24.912430792671486	26.339635340261037	24.383275109202437
62-63	23.704012024398068	24.81049996026533	26.454705909477553	25.03078210585905
64-65	23.80119711657918	25.101993812551154	26.20533961186305	24.89146945900662
66-67	23.97056024519557	25.058914829073416	25.943740998442017	25.026783927289
68-69	24.026729123574857	24.862701006192957	26.309629190314276	24.80094067991791
70-71	24.207975213645447	24.573947740074885	26.21342911425377	25.004647932025897
72-73	24.068376454105834	24.628827130031425	26.143025050305663	25.15977136555708
74-75	23.91908308685328	24.919997714045206	26.01266938937291	25.1482498097286
76-77	24.194527503636795	24.21790208136757	25.905458295019546	25.682112119976093
78-79	24.0191215047025	24.713514680218832	25.96126889677306	25.306094918305604
80-81	23.65762009295937	25.003642339436606	25.967231208839724	25.3715063587643
82-83	23.74992212537153	24.67119737575992	26.04318890103815	25.5356915978304
84-85	23.91810891830759	24.591729264663826	25.918832966975824	25.571328850052762
86-87	24.432854461669173	24.89185898549061	25.532782743745287	25.14250380909493
88-89	23.282045068463113	25.02704137219573	25.676622960614516	26.014290598726642
90-91	23.652015189279794	24.50895075551509	25.919026481168167	25.920007574036948
92-93	24.016393080744432	24.681843846315726	26.02885982782801	25.272903245111827
94-95	23.40522127813836	24.453224680568425	25.909191025158655	26.232363016134563
96-97	23.508702048472855	24.51888432081148	26.193217410670265	25.779196220045396
98-99	23.413388876270943	24.684787124922064	25.886871162393927	26.014952836413062
100	23.529108780143464	24.34532899232442	25.685354687146656	26.44020754038546
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	510.0
1	297.5
2	295.0
3	641.0
4	482.0
5	246.5
6	461.0
7	922.5
8	1499.0
9	1731.5
10	1338.0
11	879.5
12	796.0
13	892.5
14	1001.5
15	1072.0
16	1180.0
17	1298.0
18	1405.0
19	1518.5
20	1661.0
21	1802.0
22	1885.0
23	1990.5
24	2295.0
25	2824.0
26	3795.5
27	5205.0
28	6733.5
29	7800.0
30	8966.0
31	11127.5
32	13420.5
33	16149.0
34	20204.0
35	23545.5
36	25776.0
37	27794.0
38	29568.0
39	34856.5
40	43506.5
41	50552.5
42	55358.5
43	61197.5
44	70622.5
45	76835.0
46	81110.0
47	87533.5
48	97318.0
49	98486.5
50	94246.5
51	96332.0
52	88872.5
53	82350.5
54	80531.0
55	74894.0
56	69948.5
57	63306.5
58	56414.5
59	52650.5
60	48381.5
61	43246.5
62	40467.0
63	35985.5
64	28874.5
65	23380.5
66	18454.5
67	13660.5
68	10370.5
69	8298.0
70	5829.0
71	3575.5
72	2653.5
73	2125.0
74	1972.5
75	1463.5
76	861.0
77	599.5
78	347.0
79	148.5
80	78.5
81	35.0
82	28.5
83	15.0
84	3.0
85	3.5
86	3.5
87	2.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08545318887067871
2	0.0010792021556572512
3	0.0
4	9.810928687793194E-5
5	0.038115457952076555
6	4.905464343896597E-5
7	0.004414917909506937
8	4.905464343896597E-4
9	9.810928687793194E-5
10-11	3.4338250407276176E-4
12-13	0.0
14-15	2.2074589547534684E-4
16-17	0.0
18-19	4.905464343896597E-5
20-21	0.0
22-23	2.943278606337958E-4
24-25	0.0
26-27	0.0011527841208157001
28-29	0.008314762062904732
30-31	0.03377412200772807
32-33	0.024110357250251773
34-35	0.027249854430345596
36-37	0.024159411893690738
38-39	0.02376697474617901
40-41	0.029162985524465265
42-43	0.02163309775658399
44-45	0.020480313635768292
46-47	0.014446592492775478
48-49	0.010252420478743888
50-51	0.006965759368333168
52-53	0.00468471844842125
54-55	0.0034092977190081347
56-57	0.0022810409199119177
58-59	8.339289384624213E-4
60-61	6.131830429870746E-4
62-63	0.0010301475122182853
64-65	0.003016860571496407
66-67	3.4338250407276176E-4
68-69	8.584562601819044E-4
70-71	9.810928687793194E-5
72-73	2.4527321719482984E-4
74-75	3.679098257922448E-4
76-77	1.7169125203638088E-4
78-79	2.4527321719482984E-5
80-81	8.829835819013874E-4
82-83	4.905464343896597E-4
84-85	2.943278606337958E-4
86-87	4.905464343896597E-5
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2038543.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.443294413833122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.8621664802344	9.185580500290541
2	11.688905747001659	3.844082371469885
3	6.023825311128631	2.9715459926516394
4	3.9669290278300204	2.609175276935593
5	2.9698142646159322	2.4416765153740543
6	2.3131858007091863	2.2821837092975676
7	1.84399692082672	2.1224968987148833
8	1.5333208495621098	2.01702769281748
9	1.2540562398729331	1.8558734367361656
>10	10.848640981927138	36.22044187415489
>50	1.157057533140687	12.986271375726888
>100	0.49445489575631774	14.388074871019013
>500	0.03228604327785136	3.7074234133247086
>1k	0.011060959271115745	3.1123854210109547
>5k	2.989448451652904E-4	0.2557606504757091
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5203	0.2552313098129399	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4945	0.2425752118056867	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	4739	0.2324699552572597	No Hit
GTACACAGGAGGCAAAGTGTTTCACATCATAGACTTCACTTCCAACTCCT	3091	0.15162790286984382	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2712	0.1330361930064757	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	2634	0.12920993081823637	No Hit
GTACAAGAGTTACAGCCCTTATGACATGTTGGAAAGCATCAGGAAAGAGG	2578	0.12646287078565424	No Hit
CTCTTGTACCTATCAAATACTTTCTGGAGGTGGGGCACGCTCCGCTCGGT	2439	0.11964427534763798	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.905464343896597E-5	0.0
10-11	0.0	0.0	0.0	4.905464343896597E-5	0.0
12-13	0.0	0.0	0.0	4.905464343896597E-5	0.0
14-15	0.0	0.0	0.0	4.905464343896597E-5	0.0
16-17	0.0	0.0	0.0	7.358196515844896E-5	0.0
18-19	0.0	0.0	0.0	4.660191126701767E-4	0.0
20-21	0.0	0.0	0.0	6.867650081455235E-4	0.0
22-23	0.0	0.0	0.0	9.565655470598364E-4	0.0
24-25	0.0	0.0	0.0	0.001741439842083292	0.0
26-27	0.0	0.0	0.0	0.004047008083714692	0.0
28-29	0.0	0.0	0.0	0.009271327609964568	0.0
30-31	0.0	0.0	0.0	0.025851797092335066	0.0
32-33	0.0	0.0	0.0	0.05660905852856673	0.0
34-35	0.0	0.0	0.0	0.10375057087341302	0.0
36-37	0.0	0.0	0.0	0.1777249731793737	0.0
38-39	0.0	0.0	0.0	0.2888337405686316	0.0
40-41	0.0	0.0	0.0	0.41583621243211455	0.0
42-43	0.0	0.0	0.0	0.5321447720259027	0.0
44-45	0.0	0.0	0.0	0.6679034977432411	0.0
46-47	0.0	0.0	0.0	0.8337081925669461	0.0
48-49	0.0	0.0	0.0	1.0089804335743715	0.0
50-51	0.0	0.0	0.0	1.1891336116039741	0.0
52-53	0.0	0.0	0.0	1.3638171968901318	0.0
54-55	0.0	0.0	0.0	1.5502738966016416	0.0
56-57	0.0	0.0	0.0	1.7740611799702042	0.0
58-59	0.0	0.0	0.0	2.0136685858478334	0.0
60-61	0.0	0.0	0.0	2.2717205376585143	0.0
62-63	0.0	0.0	0.0	2.529797016790914	0.0
64-65	0.0	0.0	0.0	2.796163730664499	0.0
66-67	0.0	0.0	0.0	3.1040797275308885	0.0
68-69	0.0	0.0	0.0	3.448958398228539	0.0
70-71	0.0	0.0	0.0	3.817187079203137	0.0
72-73	0.0	0.0	0.0	4.187304363950135	0.0
74-75	0.0	0.0	0.0	4.56993058277407	0.0
76-77	0.0	0.0	0.0	4.982725407312968	0.0
78-79	0.0	0.0	0.0	5.445237112977258	0.0
80-81	0.0	0.0	0.0	5.939707918842036	0.0
82-83	0.0	0.0	0.0	6.462434199327657	0.0
84-85	0.0	0.0	0.0	6.986092518038618	0.0
86-87	0.0	0.0	0.0	7.5327084098790165	0.0
88	0.0	0.0	0.0	7.973979454934235	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGCAC	35	0.008844578	26.853428	78-79
GTACATG	8815	0.0	26.796217	1
GTACACA	1490	0.0	26.211018	1
CACTACG	90	9.5251744E-4	26.10878	7
TACATGG	9035	0.0	25.703114	2
ATTACGG	170	1.2420787E-7	24.88807	5
ATCCGAT	115	1.3785534E-4	24.519552	7
TTCGACG	50	0.001760861	23.496752	70-71
ACATGGG	9760	0.0	22.878668	3
GTAATCG	270	4.7293724E-11	22.626501	94
TACACTG	1125	0.0	22.565182	5
TGACACA	1025	0.0	22.466288	6
CATGGGG	6635	0.0	22.388918	4
CCTAATA	195	4.6685636E-7	21.696758	2
GTATACA	200	5.9027843E-7	21.174082	1
GTATAGC	365	0.0	20.626198	1
TATCCCG	115	0.0039544078	20.439476	5
GAGGCAA	1650	0.0	20.221943	9
GTACCTA	1255	0.0	20.221304	6
ATAGGGG	210	9.516498E-7	20.14699	3
>>END_MODULE