FastQCFastQC Report
Wed 25 May 2016
SRR1033177_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033177_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1756319
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73750.4199123279996402No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG35500.20212729008796237No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGAC30430.17326009682751253No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA26710.1520794343168866No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT26420.1504282536372948No Hit
GTACATGGGGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCCGAGGC26240.14940338287065164No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA25950.14775220219105983No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT24910.14183072665045474No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA23230.13226526616178497No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG22700.1292475911266689No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT21510.12247205661386115No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC21060.11990987969725318No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA20630.11746157731027222No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA20370.11598120842512094No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT19620.11171091356410766No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA19340.11011667014932935No Hit
GTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGT19290.10983198382526181No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG18980.10806692861604297No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA18290.10413825734391076No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC18120.10317032384208108No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT18030.10265788845875948No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA17910.10197464128099737No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC17630.10038039786621908No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA2550.066.5420761
GCTCTAG2000.065.987561
GACCGAT250.005256180756.3465587
ACCGATC250.005256180756.3465588
AACACGT7850.050.84356
ACACGTG8250.048.378367
ACGTGCG8250.048.378369
GAGAACA5600.047.133971
CACGTGC8750.045.613888
ACGCAGT1350.045.2163738
CGCAGAA1800.044.3973962
TACATGA2000.042.308112
TAGATGG4550.040.247547
ATAGATG4700.038.9630476
AGAACAA6150.035.9255872
AACGCGC850.035.9091716-17
GGTATCA27300.035.2209851
GGCACTA702.197671E-433.539628
TAACACG12550.031.445375
TACATGG81400.030.4923292
Produced by FastQC (version 0.11.5)