##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033177_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1756319
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.974501784698564	33.0	31.0	34.0	28.0	34.0
2	31.029470158894824	33.0	31.0	34.0	28.0	34.0
3	31.230090319583173	34.0	31.0	34.0	28.0	34.0
4	34.46587892062888	37.0	35.0	37.0	32.0	37.0
5	34.80770862240857	37.0	35.0	37.0	32.0	37.0
6	34.74502240196684	37.0	35.0	37.0	32.0	37.0
7	34.728732650503694	37.0	35.0	37.0	32.0	37.0
8	34.70241909357013	37.0	35.0	37.0	32.0	37.0
9	36.24401660518391	39.0	37.0	39.0	32.0	39.0
10-11	36.303133713180806	39.0	37.0	39.0	32.0	39.0
12-13	36.30409538358351	39.0	37.0	39.0	32.0	39.0
14-15	37.63746563124353	40.5	38.0	41.0	32.0	41.0
16-17	37.6284447756928	40.0	38.0	41.0	32.0	41.0
18-19	37.62193286071608	40.0	38.0	41.0	32.0	41.0
20-21	37.62967917559395	40.0	38.0	41.0	32.0	41.0
22-23	37.603014031050165	40.0	38.0	41.0	31.5	41.0
24-25	37.61674786869584	40.0	38.0	41.0	31.0	41.0
26-27	37.5551924223333	40.0	38.0	41.0	31.0	41.0
28-29	37.51529961242804	40.0	38.0	41.0	31.0	41.0
30-31	37.43131486933752	40.0	38.0	41.0	30.0	41.0
32-33	37.47471045977411	40.0	38.0	41.0	30.0	41.0
34-35	37.43414749826199	40.0	38.0	41.0	30.0	41.0
36-37	37.29638465449614	40.0	38.0	41.0	30.0	41.0
38-39	37.18767234198343	40.0	38.0	41.0	30.0	41.0
40-41	37.11445528972812	40.0	38.0	41.0	30.0	41.0
42-43	36.93653459308929	40.0	37.5	41.0	30.0	41.0
44-45	36.78553212713636	40.0	37.0	41.0	30.0	41.0
46-47	36.62837218068016	40.0	37.0	41.0	30.0	41.0
48-49	36.45999900929159	40.0	36.5	41.0	29.5	41.0
50-51	36.31019478807666	40.0	36.0	41.0	29.0	41.0
52-53	36.167211081813726	40.0	35.5	41.0	29.0	41.0
54-55	35.945181655496526	39.0	35.0	41.0	29.0	41.0
56-57	35.76362665324466	39.0	35.0	41.0	28.0	41.0
58-59	35.467321141546606	39.0	35.0	41.0	28.0	41.0
60-61	35.17314366012097	38.5	35.0	40.0	28.0	41.0
62-63	34.80940136729148	37.5	35.0	40.0	27.0	41.0
64-65	34.471208248615426	37.0	34.0	39.5	27.0	41.0
66-67	34.05044100758461	36.5	34.0	39.0	26.5	41.0
68-69	33.6504034859271	36.0	34.0	39.0	26.0	40.5
70-71	33.24854767271777	35.0	34.0	37.5	26.0	40.0
72-73	32.80238925844337	35.0	33.0	37.0	25.5	39.0
74-75	32.39334767772825	35.0	33.0	36.5	25.0	39.0
76-77	32.11173169566577	35.0	33.0	36.0	25.0	37.5
78-79	31.790686942406246	35.0	33.0	36.0	24.5	37.0
80-81	31.48341332070085	35.0	33.0	35.0	24.0	37.0
82-83	31.363685640251003	35.0	33.0	35.0	24.0	36.0
84-85	31.165308807796308	35.0	33.0	35.0	23.0	36.0
86-87	31.009277357928713	35.0	32.5	35.0	23.0	36.0
88-89	30.735246558284686	35.0	32.0	35.0	19.5	35.0
90-91	30.59082803294846	35.0	32.0	35.0	18.0	35.0
92-93	30.45145870425589	35.0	32.0	35.0	12.5	35.0
94-95	30.278163306324192	35.0	32.0	35.0	4.5	35.0
96-97	30.08701095871536	35.0	32.0	35.0	2.0	35.0
98-99	29.842586398029063	35.0	31.5	35.0	2.0	35.0
100	29.65865597308917	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.24315098247276978
1101	2	0.19158014591582528
1101	3	0.18114774880320894
1101	4	0.12191313370140477
1101	5	0.07898729319435205
1101	6	0.04346944014773868
1101	7	0.094651885992171
1101	8	0.07120516703731994
1101	9	0.17613144963549843
1101	10-11	0.12432218677432871
1101	12-13	0.11348657044366917
1101	14-15	0.03367831181886771
1101	16-17	0.020330543516635657
1101	18-19	0.07339326421047332
1101	20-21	0.06132752977424616
1101	22-23	0.09278959935131326
1101	24-25	0.13412663235257583
1101	26-27	0.20332711682799953
1101	28-29	0.28211690334251927
1101	30-31	0.3401622890018885
1101	32-33	0.25054392968260686
1101	34-35	0.24882427831929022
1101	36-37	0.3377388504181269
1101	38-39	0.2735421569293379
1101	40-41	0.2254718720526725
1101	42-43	0.2258737902758341
1101	44-45	0.20300010012169167
1101	46-47	0.23676551021700476
1101	48-49	0.30025570509080524
1101	50-51	0.22167656626783838
1101	52-53	0.21780338346440686
1101	54-55	0.20706319142782093
1101	56-57	0.1918948095258557
1101	58-59	0.21805543494037494
1101	60-61	0.20370069398092738
1101	62-63	0.20404155280885306
1101	64-65	0.188854459157632
1101	66-67	0.140944019696164
1101	68-69	0.14930741769817502
1101	70-71	0.2331688915193766
1101	72-73	0.2343248608546986
1101	74-75	0.13538783617514127
1101	76-77	0.13862001380888245
1101	78-79	0.17324113308738376
1101	80-81	0.19789056558618512
1101	82-83	0.2740099818868913
1101	84-85	0.23573174998661628
1101	86-87	0.3159090803717639
1101	88-89	0.3546123843167699
1101	90-91	0.47654768236561296
1101	92-93	0.4988141768021066
1101	94-95	0.48152935196392477
1101	96-97	0.42607766383368073
1101	98-99	0.4422639196075373
1101	100	0.46812776393796085
1102	1	0.49282336829610784
1102	2	0.4167038037647295
1102	3	0.3588455697122832
1102	4	0.34281298305133134
1102	5	0.2707738800930386
1102	6	0.2867353094963292
1102	7	0.2841573811932534
1102	8	0.2372439743517134
1102	9	0.3729055848443892
1102	10-11	0.3843308315204723
1102	12-13	0.43594853265636146
1102	14-15	0.4855523046969026
1102	16-17	0.45811142994674015
1102	18-19	0.5152027289415386
1102	20-21	0.4644502182541288
1102	22-23	0.4853243727522596
1102	24-25	0.42226994705207943
1102	26-27	0.4144479135914665
1102	28-29	0.4233730500644768
1102	30-31	0.45241974328691015
1102	32-33	0.41563163406846115
1102	34-35	0.5048718931470262
1102	36-37	0.5729778559467888
1102	38-39	0.49342751457406564
1102	40-41	0.5553290709740679
1102	42-43	0.6047456147332255
1102	44-45	0.5036323602164288
1102	46-47	0.5882057285420572
1102	48-49	0.5504983186254435
1102	50-51	0.5663180896240263
1102	52-53	0.5265022949136338
1102	54-55	0.4974418421601854
1102	56-57	0.5031633627245
1102	58-59	0.49700683446690164
1102	60-61	0.4480835983624374
1102	62-63	0.5494281881759946
1102	64-65	0.5087947959410215
1102	66-67	0.5030030788656532
1102	68-69	0.5414451295893983
1102	70-71	0.5789315190881581
1102	72-73	0.624705591389997
1102	74-75	0.5298611667715747
1102	76-77	0.5212518507089214
1102	78-79	0.5934575411049288
1102	80-81	0.5641042572002348
1102	82-83	0.6935734222064909
1102	84-85	0.6989150782862268
1102	86-87	0.7454469843282752
1102	88-89	0.8506861684910945
1102	90-91	0.8677379083768955
1102	92-93	0.905983800104579
1102	94-95	0.9016492752101541
1102	96-97	0.8618528968168242
1102	98-99	0.9456492058155526
1102	100	1.038064483672759
1103	1	0.3448112824627252
1103	2	0.24616259466782964
1103	3	0.266403495262864
1103	4	0.27129857334842455
1103	5	0.26991451913576014
1103	6	0.3127112918982604
1103	7	0.2656794087373129
1103	8	0.26342281387204736
1103	9	0.32153480140506474
1103	10-11	0.24029157824009673
1103	12-13	0.32783168010866603
1103	14-15	0.32902278770226445
1103	16-17	0.3925068386995534
1103	18-19	0.42099528399969444
1103	20-21	0.4370786302338274
1103	22-23	0.3356671756042431
1103	24-25	0.3833705967084171
1103	26-27	0.4379078685691624
1103	28-29	0.4324819338367689
1103	30-31	0.47534045782329315
1103	32-33	0.5349355859305476
1103	34-35	0.58219426477784
1103	36-37	0.5463295348676169
1103	38-39	0.6088219732365729
1103	40-41	0.7107818189604771
1103	42-43	0.5510830594182821
1103	44-45	0.5131572294720215
1103	46-47	0.5687434282964006
1103	48-49	0.5146392572461878
1103	50-51	0.5168586139711593
1103	52-53	0.3615824534332006
1103	54-55	0.550104234847062
1103	56-57	0.49927697982955266
1103	58-59	0.4671414733897876
1103	60-61	0.4217387070236711
1103	62-63	0.5594366619543578
1103	64-65	0.5624933006187192
1103	66-67	0.5247661383276068
1103	68-69	0.5659223659393433
1103	70-71	0.5840487396888534
1103	72-73	0.5336959278589148
1103	74-75	0.5371374889805764
1103	76-77	0.4950828544664816
1103	78-79	0.44470849333507445
1103	80-81	0.4633065237219611
1103	82-83	0.49154356711666125
1103	84-85	0.5804396736419122
1103	86-87	0.5192925390183838
1103	88-89	0.5879879120351887
1103	90-91	0.5640535266631375
1103	92-93	0.5395955983430625
1103	94-95	0.5826620201151869
1103	96-97	0.6069265502136112
1103	98-99	0.7260593709258387
1103	100	0.7851144960838461
1104	1	0.3810359909501919
1104	2	0.28178122882506074
1104	3	0.33265678483764205
1104	4	0.33634683122843967
1104	5	0.2127219356610297
1104	6	0.3425242612742778
1104	7	0.2814467227782771
1104	8	0.3064117130230244
1104	9	0.3806591558234871
1104	10-11	0.4078541468824213
1104	12-13	0.4092978073955962
1104	14-15	0.39344701708854046
1104	16-17	0.4764209595682729
1104	18-19	0.48893311083421764
1104	20-21	0.4207488332776137
1104	22-23	0.4217486551567191
1104	24-25	0.3961784661315093
1104	26-27	0.4729607848267463
1104	28-29	0.47643453484510445
1104	30-31	0.47299792473344837
1104	32-33	0.4664085875593642
1104	34-35	0.48540231794804356
1104	36-37	0.49915464675764554
1104	38-39	0.42922590956561635
1104	40-41	0.4709850406814553
1104	42-43	0.4540334460797979
1104	44-45	0.430271429274363
1104	46-47	0.39027402882273776
1104	48-49	0.33103245989693164
1104	50-51	0.39317060233815937
1104	52-53	0.4068436865122109
1104	54-55	0.33906048330032945
1104	56-57	0.30992015943103723
1104	58-59	0.4041034348255437
1104	60-61	0.3927008399135943
1104	62-63	0.405962207817538
1104	64-65	0.3494057667929198
1104	66-67	0.3208983909604868
1104	68-69	0.3776029241682508
1104	70-71	0.39710877614712814
1104	72-73	0.4256185761465545
1104	74-75	0.5238824638366566
1104	76-77	0.338262051575704
1104	78-79	0.3527878669126956
1104	80-81	0.42760361822071147
1104	82-83	0.49979301999992387
1104	84-85	0.46976519556620744
1104	86-87	0.5284825636204395
1104	88-89	0.5407577865663171
1104	90-91	0.5457343760406488
1104	92-93	0.5300916993780973
1104	94-95	0.6238074633970143
1104	96-97	0.6134511902811575
1104	98-99	0.574542567608038
1104	100	0.5807712060076469
1105	1	0.26061983074291106
1105	2	0.24224610324901263
1105	3	0.224370238704779
1105	4	0.17284425144236337
1105	5	0.21402245591618652
1105	6	0.14249182277605144
1105	7	0.16271632920737744
1105	8	0.16110747583046248
1105	9	0.22873514347615043
1105	10-11	0.2609516705293444
1105	12-13	0.22285432324363796
1105	14-15	0.2795890647703132
1105	16-17	0.3259918945483804
1105	18-19	0.30861976560780846
1105	20-21	0.359783201301795
1105	22-23	0.36717268577815787
1105	24-25	0.4309961274193128
1105	26-27	0.46835704351341434
1105	28-29	0.4605664152223454
1105	30-31	0.44641184608828155
1105	32-33	0.48505186148852175
1105	34-35	0.46690939363467976
1105	36-37	0.4637420182369638
1105	38-39	0.40260516502051047
1105	40-41	0.3982599825219566
1105	42-43	0.3478496769678756
1105	44-45	0.33481118768911955
1105	46-47	0.4050263299875212
1105	48-49	0.4004559440206492
1105	50-51	0.442373906233712
1105	52-53	0.4217868854155782
1105	54-55	0.405463798928956
1105	56-57	0.3967091418237416
1105	58-59	0.36774486644250715
1105	60-61	0.31602316329436064
1105	62-63	0.43903018231410584
1105	64-65	0.31317950920896465
1105	66-67	0.4286443215995064
1105	68-69	0.4010166023912163
1105	70-71	0.36092675287893883
1105	72-73	0.35327147041195417
1105	74-75	0.37855336444889787
1105	76-77	0.39474817478458846
1105	78-79	0.36146791022467895
1105	80-81	0.2974839079272442
1105	82-83	0.3441857165692994
1105	84-85	0.2661316602451862
1105	86-87	0.31565668259286994
1105	88-89	0.310862008574901
1105	90-91	0.4198355135684295
1105	92-93	0.40420522878866194
1105	94-95	0.4345020033295448
1105	96-97	0.4799560252330437
1105	98-99	0.5501365976067483
1105	100	0.5934774347796861
1106	1	0.36091937063864066
1106	2	0.2767244441251506
1106	3	0.28172700858570465
1106	4	0.3007639379265967
1106	5	0.19356254078508783
1106	6	0.19209551720486928
1106	7	0.1147466499563663
1106	8	0.21991768876519302
1106	9	0.17786404552227708
1106	10-11	0.09845929349534543
1106	12-13	0.12107784523050924
1106	14-15	0.1820293596023319
1106	16-17	0.1925482082999821
1106	18-19	0.2337944898252644
1106	20-21	0.20933440864514097
1106	22-23	0.20730850497788111
1106	24-25	0.2545269951304334
1106	26-27	0.2568184995660445
1106	28-29	0.29796018317888695
1106	30-31	0.2652442182666448
1106	32-33	0.24715584591144335
1106	34-35	0.23573909860613185
1106	36-37	0.2172819042105445
1106	38-39	0.2409268402215048
1106	40-41	0.24777934592690798
1106	42-43	0.2674225278455893
1106	44-45	0.17256336619130508
1106	46-47	0.17480898882782014
1106	48-49	0.12106845885098494
1106	50-51	0.11301360293165175
1106	52-53	0.07873346695031813
1106	54-55	0.11952806221315626
1106	56-57	0.13140317920512956
1106	58-59	0.037103638871244016
1106	60-61	0.14277869154245337
1106	62-63	0.15597517682379447
1106	64-65	0.13913517092083083
1106	66-67	0.15144076908678983
1106	68-69	0.14405803689636087
1106	70-71	0.06372727579925908
1106	72-73	0.17611226298467386
1106	74-75	0.16302952575152574
1106	76-77	0.21069168851419562
1106	78-79	0.19570403029420547
1106	80-81	0.2431090162594387
1106	82-83	0.2077676882602617
1106	84-85	0.10731086714345395
1106	86-87	0.22278579417358557
1106	88-89	0.22220080015137
1106	90-91	0.1895712561653795
1106	92-93	0.21951023830563798
1106	94-95	0.15633906905321382
1106	96-97	0.07664552644170897
1106	98-99	0.27615452862553624
1106	100	0.34715045944698986
1107	1	0.22510768056840647
1107	2	0.2241657917038502
1107	3	0.26645578323513774
1107	4	0.20445064171121174
1107	5	0.2016742334940318
1107	6	0.10706717877696548
1107	7	0.2236548184509246
1107	8	0.18053442628371386
1107	9	0.20003398720663768
1107	10-11	0.1599570846117544
1107	12-13	0.20494598161605637
1107	14-15	0.1654973832174491
1107	16-17	0.1642265154104905
1107	18-19	0.2114639336254598
1107	20-21	0.18282238929084826
1107	22-23	0.0736730617359811
1107	24-25	0.07752181352422838
1107	26-27	0.09595872235705372
1107	28-29	0.0998609618780506
1107	30-31	0.12936364380414034
1107	32-33	0.1371212231715404
1107	34-35	0.126705521631294
1107	36-37	0.07511854542979535
1107	38-39	0.12424244323075584
1107	40-41	0.2403723294404827
1107	42-43	0.11892222161557697
1107	44-45	0.17390688960019673
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2216	1	-1.1108644040288809
2216	2	-1.079565583336425
2216	3	-1.0340700622210122
2216	4	-1.04144745977392
2216	5	-1.0035562570316259
2216	6	-0.9353007671430191
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2216	10-11	-1.117302683043583
2216	12-13	-1.1972595297306654
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2216	38-39	-1.5888152417118278
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2216	54-55	-1.555587125730284
2216	56-57	-1.7574048495696957
2216	58-59	-1.6536770473767035
2216	60-61	-1.6432026985749815
2216	62-63	-1.542897776346443
2216	64-65	-1.416048209029178
2216	66-67	-1.466744510045956
2216	68-69	-1.5565469361970017
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2216	80-81	-1.4642245076328777
2216	82-83	-1.5997166293763456
2216	84-85	-1.6224937148286322
2216	86-87	-1.7059784225701016
2216	88-89	-1.6334649089667508
2216	90-91	-1.580186413575241
2216	92-93	-1.5556254729640244
2216	94-95	-1.6340752355191448
2216	96-97	-1.6547866607062254
2216	98-99	-1.6876424479350867
2216	100	-1.7812402451603404
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	27092.0
3	4375.0
4	3720.0
5	4198.0
6	4490.0
7	7373.0
8	7675.0
9	5698.0
10	4126.0
11	3821.0
12	4061.0
13	4177.0
14	4308.0
15	4232.0
16	4214.0
17	4112.0
18	3804.0
19	3793.0
20	3763.0
21	3987.0
22	5958.0
23	9492.0
24	13450.0
25	15230.0
26	15220.0
27	11903.0
28	13566.0
29	16727.0
30	21421.0
31	26595.0
32	35765.0
33	48860.0
34	71991.0
35	118276.0
36	223462.0
37	447063.0
38	489464.0
39	58837.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.83957281875073	20.893607419403672	12.79218757586923	23.47463218597637
2	17.23660471254179	21.035311285987497	37.894946857782934	23.83313714368778
3	17.001559420649876	29.31323388062142	28.280495163061158	25.404711535667545
4	11.998578270320026	19.180591567137995	38.944172047518585	29.876658115023396
5	11.012426917240468	42.458918716933326	35.53513896547167	10.993515400354534
6	27.610827789001842	41.47577539022907	17.55222225006093	13.361174570708165
7	27.44309676155617	34.15714649327548	23.276617527899994	15.123139217268362
8	25.376983720225688	37.05368430403794	18.745600907494346	18.823731068242026
9	26.373954293589367	18.383646831701775	19.82670744138634	35.415691433322515
10-11	24.33893349484305	28.4689351492356	27.74760860546098	19.44452275046037
12-13	24.21988292371249	28.043469533201083	25.766748887906243	21.96989865518018
14-15	22.694408424692522	28.20007733568871	24.134999182792978	24.970515056825786
16-17	21.846356496528113	30.291077079730133	25.339401045922997	22.523165377818756
18-19	21.996227140385972	30.04412110167501	27.039615025947306	20.920036731991715
20-21	23.013533939068797	28.94716159741942	26.882045604431276	21.157258859080507
22-23	22.908195297651673	28.312977370927445	26.833225372464998	21.945601958955887
24-25	21.714529631716818	30.062331155012906	26.26437077825241	21.95876843501787
26-27	21.086562645664664	29.983294033831648	26.54716694775705	22.382976372746636
28-29	22.823215992145617	28.51024111000124	27.817828631826885	20.848714266026256
30-31	22.67772695381195	29.13866666059209	26.648991544187993	21.534614841407965
32-33	21.71759267301093	28.98754431221771	26.78438455814785	22.510478456623503
34-35	23.22246718675836	28.553165039564952	26.719547116475084	21.504820657201602
36-37	23.521736171870174	28.496853467506426	25.718030942302704	22.263379418320696
38-39	22.341522637752117	29.309378721019826	26.658432375456346	21.690666265771714
40-41	22.10701011825414	28.95091089342322	27.113606999014312	21.828471989308326
42-43	21.979872602978027	29.318056092157708	26.8216769110621	21.88039439380217
44-45	21.026596786610728	29.08970863569896	26.935168481999277	22.948526095691033
46-47	22.325325816944616	28.578277921319923	26.516111122974102	22.58028513876136
48-49	21.91022272076345	28.74159779300606	26.956301406133004	22.391878080097484
50-51	21.97131225602307	28.386010475544865	26.366719233247288	23.27595803518478
52-53	21.95030149424643	28.751865921825882	26.575310640573136	22.722521943354558
54-55	21.02628955233569	30.133312531423506	26.810349785252548	22.03004813098826
56-57	21.99129629218403	29.011628668371426	26.2524177756976	22.744657263746944
58-59	22.349592144206603	27.54791721945181	26.79999180074909	23.30249883559249
60-61	22.34763840849906	28.243923173436	27.054565103944988	22.353873314119955
62-63	21.210786588507933	28.548217656319842	26.96208694067775	23.27890881449448
64-65	21.486390882937823	29.30738056702638	26.137931721676793	23.068296828359006
66-67	21.386485769369614	27.85905110910435	26.92306816331318	23.83139495821286
68-69	21.68925163002909	29.132780680731805	26.603167987373144	22.57479970186596
70-71	22.334100774397093	28.20546618841812	26.992070408328562	22.46836262885622
72-73	21.568673225248453	27.81166264489482	26.392664242595405	24.22699988726133
74-75	21.093771352045696	27.95822628841091	27.39293799593685	23.555064363606547
76-77	21.383157901929234	28.111444778111444	27.00587390576014	23.49952341419918
78-79	21.610304481918956	28.51170294813567	26.903129431506017	22.974863138439357
80-81	21.69519532001781	28.475652044189708	26.837669665866855	22.991482969925624
82-83	21.42232848289669	28.370556202128604	26.969118608321736	23.23799670665297
84-85	21.149230777114685	27.990642578261788	27.118757946605644	23.74136869801788
86-87	21.902690139193037	27.327921852384545	27.345402120179973	23.42398588824244
88-89	21.7497879029343	28.019927099331316	27.349551431175435	22.880733566558952
90-91	21.347406643104843	27.910447504667772	27.50462766953942	23.23751818268796
92-93	21.288454024746695	28.175575729035256	27.282922167873753	23.253048078344293
94-95	21.848138048716642	26.910976380600292	27.568701724921592	23.672183845761474
96-97	22.10157462725039	27.761096568355093	26.835623578739952	23.30170522565457
98-99	20.86346161336367	27.665722723596513	27.109552383112582	24.361263279927233
100	20.865357407570762	27.24825325835051	28.321959512481797	23.56442982159693
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5614.0
1	4509.0
2	3147.0
3	2404.5
4	1522.0
5	936.0
6	715.5
7	683.0
8	703.5
9	714.0
10	613.0
11	472.0
12	410.5
13	421.5
14	430.5
15	417.5
16	406.0
17	407.0
18	453.0
19	508.5
20	589.0
21	685.5
22	821.0
23	1087.0
24	1310.5
25	1516.5
26	1869.5
27	2263.5
28	2599.5
29	3206.0
30	4199.0
31	5405.5
32	8357.5
33	11829.0
34	15138.5
35	23257.0
36	34639.5
37	44937.0
38	57439.0
39	64209.0
40	72243.5
41	82897.0
42	94574.0
43	114470.0
44	118662.5
45	113102.5
46	115409.0
47	112476.0
48	105918.0
49	99205.5
50	94483.5
51	84432.5
52	60309.0
53	38517.5
54	26738.5
55	22511.5
56	17497.0
57	10859.5
58	7881.0
59	6125.0
60	4829.0
61	3918.0
62	2905.0
63	2230.5
64	1957.0
65	1670.0
66	1320.0
67	1154.0
68	1121.5
69	1307.5
70	1643.5
71	1906.5
72	2582.5
73	2619.5
74	1691.0
75	2482.5
76	3484.0
77	2191.5
78	1145.0
79	2080.0
80	2183.0
81	1464.5
82	1313.0
83	619.0
84	65.0
85	23.5
86	12.5
87	9.5
88	5.5
89	4.0
90	3.0
91	5.0
92	5.0
93	3.5
94	3.5
95	1.5
96	1.5
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3287557670332098
2	0.11751851457508573
3	0.06718597247994243
4	0.040482395282405985
5	0.04412638023047066
6	0.0097362722831103
7	0.007743468014637433
8	0.015657747823715394
9	0.018732360123644962
10-11	0.02206319011523533
12-13	0.016198651839443745
14-15	0.01924479550696656
16-17	0.020383540803236767
18-19	0.020298134906016504
20-21	0.024881584723504102
22-23	0.016027840045003214
24-25	0.025934924122554045
26-27	0.020468946700457034
28-29	0.02032660353842326
30-31	0.020895976186558363
32-33	0.025877986857740537
34-35	0.016084777309816726
36-37	0.01022023903402514
38-39	0.01412044167375061
40-41	0.010590331255312958
42-43	0.008853744678500888
44-45	0.009508523223856258
46-47	0.008910681943314397
48-49	0.004782730244334885
50-51	0.007601124852603656
52-53	0.008512121089619823
54-55	0.003985608536945737
56-57	0.0031884868295565896
58-59	0.0034731731536241425
60-61	0.004412638023047066
62-63	0.0036439849480646744
64-65	0.0029322691378957924
66-67	0.00210667879809989
68-69	0.0035301104184376525
70-71	0.002818394608268771
72-73	0.002562176916607974
74-75	0.002676051446234995
76-77	0.004384169390640311
78-79	0.004184888963793024
80-81	0.004156420331386268
82-83	0.002903800505489037
84-85	0.0037578594776916953
86-87	0.003017675035116058
88-89	0.6067234938527682
90-91	0.6171999505784541
92-93	0.6145238991322192
94-95	0.5995209298538591
96-97	0.5983537159251822
98-99	0.5950513545659986
100	0.59767046874742
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1756319.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.60682140479261
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.48067473175814	10.87925913915241
2	8.701744736006942	2.5420966253789525
3	3.5648461194777776	1.5621321180833967
4	2.0946927979332277	1.2238721438926399
5	1.3492269275984519	0.985395838298382
6	0.9805793545986063	0.8593888503509188
7	0.7953392548351645	0.813216491611866
8	0.6376561535199685	0.7451303641706559
9	0.5132885635987368	0.6747762939848353
>10	4.678442100683082	14.60969863997982
>50	0.9510873339396764	9.825121421092115
>100	1.0829212142539417	32.914239689393014
>500	0.12124009246538694	12.058731322695074
>1k	0.04786825657209452	9.884267072082574
>5k	3.9236275878766E-4	0.4226739898333515
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7375	0.4199123279996402	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG	3550	0.20212729008796237	No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGAC	3043	0.17326009682751253	No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA	2671	0.1520794343168866	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT	2642	0.1504282536372948	No Hit
GTACATGGGGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCCGAGGC	2624	0.14940338287065164	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA	2595	0.14775220219105983	No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT	2491	0.14183072665045474	No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA	2323	0.13226526616178497	No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG	2270	0.1292475911266689	No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT	2151	0.12247205661386115	No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC	2106	0.11990987969725318	No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA	2063	0.11746157731027222	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA	2037	0.11598120842512094	No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT	1962	0.11171091356410766	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA	1934	0.11011667014932935	No Hit
GTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGT	1929	0.10983198382526181	No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG	1898	0.10806692861604297	No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA	1829	0.10413825734391076	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC	1812	0.10317032384208108	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT	1803	0.10265788845875948	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA	1791	0.10197464128099737	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC	1763	0.10038039786621908	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	5.693726481351053E-5	0.0
9	0.0	0.0	0.0	5.693726481351053E-5	0.0
10-11	0.0	0.0	0.0	5.693726481351053E-5	0.0
12-13	0.0	0.0	0.0	1.1387452962702106E-4	0.0
14-15	0.0	0.0	0.0	1.7081179444053158E-4	0.0
16-17	0.0	0.0	0.0	3.7009222128781845E-4	0.0
18-19	0.0	0.0	0.0	5.124353833215948E-4	0.0
20-21	0.0	0.0	0.0	6.832471777621263E-4	0.0
22-23	0.0	0.0	0.0	0.0011387452962702107	0.0
24-25	0.0	0.0	0.0	0.0030746122999295686	0.0
26-27	0.0	0.0	0.0	0.006177693232265893	0.0
28-29	0.0	0.0	0.0	0.0129816963774804	0.0
30-31	0.0	0.0	0.0	0.03450398247698738	0.0
32-33	0.0	0.0	0.0	0.07646674664454464	0.0
34-35	0.0	0.0	0.0	0.13326166829602137	0.0
36-37	0.0	0.0	0.0	0.2244466978948585	0.0
38-39	0.0	0.0	0.0	0.3675585130036172	0.0
40-41	0.0	0.0	0.0	0.5250469874777873	0.0
42-43	0.0	0.0	0.0	0.678521384782605	0.0
44-45	0.0	0.0	0.0	0.8511267030647622	0.0
46-47	0.0	0.0	0.0	1.0637304498784106	0.0
48-49	0.0	0.0	0.0	1.2890312067454717	0.0
50-51	0.0	0.0	0.0	1.519769472402223	0.0
52-53	0.0	0.0	0.0	1.745554196020199	0.0
54-55	0.0	0.0	0.0	1.9893310953192445	0.0
56-57	0.0	0.0	0.0	2.2715121797350024	0.0
58-59	0.0	0.0	0.0	2.585065697063005	0.0
60-61	0.0	0.0	0.0	2.915415707510993	0.0
62-63	0.0	0.0	0.0	3.2409260504498327	0.0
64-65	0.0	0.0	0.0	3.5756602302884612	0.0
66-67	0.0	0.0	0.0	3.9514177094252236	0.0
68-69	0.0	0.0	0.0	4.375799612712724	0.0
70-71	0.0	0.0	0.0	4.8361943359947706	0.0
72-73	0.0	0.0	0.0	5.307691825915452	0.0
74-75	0.0	0.0	0.0	5.796811399295914	0.0
76-77	0.0	0.0	0.0	6.27536911005347	0.0
78-79	0.0	0.0	0.0	6.811860487758773	0.0
80-81	0.0	0.0	0.0	7.38903354117333	0.0
82-83	0.0	0.0	0.0	8.00845404507951	0.0
84-85	0.0	0.0	0.0	8.657766613012784	0.0
86-87	0.0	0.0	0.0	9.296659661485187	0.0
88	0.0	0.0	0.0	9.810746225486373	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAA	255	0.0	66.542076	1
GCTCTAG	200	0.0	65.98756	1
GACCGAT	25	0.0052561807	56.346558	7
ACCGATC	25	0.0052561807	56.346558	8
AACACGT	785	0.0	50.8435	6
ACACGTG	825	0.0	48.37836	7
ACGTGCG	825	0.0	48.37836	9
GAGAACA	560	0.0	47.13397	1
CACGTGC	875	0.0	45.61388	8
ACGCAGT	135	0.0	45.216373	8
CGCAGAA	180	0.0	44.397396	2
TACATGA	200	0.0	42.30811	2
TAGATGG	455	0.0	40.24754	7
ATAGATG	470	0.0	38.963047	6
AGAACAA	615	0.0	35.925587	2
AACGCGC	85	0.0	35.90917	16-17
GGTATCA	2730	0.0	35.220985	1
GGCACTA	70	2.197671E-4	33.53962	8
TAACACG	1255	0.0	31.44537	5
TACATGG	8140	0.0	30.492329	2
>>END_MODULE