FastQCFastQC Report
Wed 25 May 2016
SRR1033177_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033177_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1756319
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97430.5547397710780331No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85160.4848777471518557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49330.28087152732504744No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG38280.21795584970611834No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGAC29920.17035629632202348No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA28490.1622142674536915No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT27410.15606504285383235No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA27050.15401530132054597No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT26630.15162393619837852No Hit
GTACATGGGGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCCGAGGC24820.14131829126713313No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA24280.13824367896720355No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG23000.1309557090710742No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC21390.12178880943609903No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA21110.12019456602132073No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT20930.11916969525467754No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA19870.11313434518444541No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19770.11256497253631031No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT19330.11005973288451586No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA19240.10954729750119425No Hit
GTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGT18740.10670043426051874No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC18610.10596024981794308No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18360.10453681819760532No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG18300.10419519460872428No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT17840.10157608042730279No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTAG2650.072.810661
GGTATCA45850.056.5547751
TAACGCA405.673405E-447.006954
GTATCAA92400.041.916491
CCGTGCG802.4616565E-741.120549
TTAAGAC704.631036E-640.292822
TACACGG704.631036E-640.292822
AACACGT7450.039.109856
ACGTGCG7800.037.9574249
GTATCAC500.001691539937.6484451
ACACGTG7750.037.5959247
CACGTGC8200.036.105848
CACGCAC801.159858E-535.2461788
CCGCGCC7900.035.09646294
GTACACG702.1690056E-433.614681
ATCAACG117000.032.824513
GGGGTAC1151.1389966E-732.692117
CAACGCA118150.032.5457275
TCAACGC118500.032.448684
TGGGAAT4650.032.340586
Produced by FastQC (version 0.11.5)