FastQCFastQC Report
Wed 25 May 2016
SRR1033176_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033176_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2784801
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31750.033.537711
TCAACGC30750.031.7775974
GGTATCA20200.031.0219361
ATCAACG31500.030.728783
CAACGCA32300.030.3970185
AACGCAG35100.028.0954256
GTACATG72600.027.6465951
TACATGG72450.025.6319452
TATCAAC41250.024.6179942
TACACCG2900.024.2985575
ACATGGG74650.024.1707483
ACGCAGA43100.022.0088587
CGCAGAG42850.021.918878
TATACCG2458.554707E-921.091815
CATGGGG47600.020.7259854
GTACTAG6700.018.283831
GCAGAGT54600.017.029889
CCTACAC11150.016.435333
GTATAGA11500.016.3881841
TAGACTG13600.015.8893515
Produced by FastQC (version 0.11.5)