##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033176_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2784801
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.512115587433357	33.0	31.0	34.0	30.0	34.0
2	31.624279436843064	34.0	31.0	34.0	30.0	34.0
3	31.814362677979503	34.0	31.0	34.0	30.0	34.0
4	35.15966957782621	37.0	35.0	37.0	33.0	37.0
5	35.265874294069846	37.0	35.0	37.0	33.0	37.0
6	35.3409313627796	37.0	36.0	37.0	33.0	37.0
7	35.26167219848025	37.0	36.0	37.0	33.0	37.0
8	35.28162335477472	37.0	36.0	37.0	33.0	37.0
9	36.93461974482198	39.0	38.0	39.0	34.0	39.0
10-11	36.917287447110226	39.0	38.0	39.0	34.0	39.0
12-13	36.868122354164626	39.0	38.0	39.0	34.0	39.0
14-15	38.31445388736933	41.0	39.0	41.0	34.0	41.0
16-17	38.322545847979804	41.0	39.0	41.0	34.0	41.0
18-19	38.31840425940669	41.0	39.0	41.0	34.0	41.0
20-21	38.348595285623645	41.0	39.0	41.0	34.0	41.0
22-23	38.307535798787775	41.0	39.0	41.0	34.0	41.0
24-25	38.300084278912564	41.0	39.0	41.0	34.0	41.0
26-27	38.250381086476196	40.0	39.0	41.0	34.0	41.0
28-29	38.21926037084876	40.0	38.5	41.0	33.5	41.0
30-31	38.21554179275287	40.0	39.0	41.0	33.0	41.0
32-33	38.279835794370946	41.0	39.0	41.0	34.0	41.0
34-35	38.25033925224818	41.0	39.0	41.0	33.5	41.0
36-37	38.1730644308157	41.0	38.5	41.0	33.0	41.0
38-39	38.098212224140966	41.0	38.0	41.0	33.0	41.0
40-41	38.04017324756778	41.0	38.0	41.0	33.0	41.0
42-43	37.935134862419254	40.0	38.0	41.0	33.0	41.0
44-45	37.82690414862678	40.0	38.0	41.0	33.0	41.0
46-47	37.71085653876166	40.0	38.0	41.0	32.5	41.0
48-49	37.600568047770736	40.0	38.0	41.0	32.0	41.0
50-51	37.48701433244243	40.0	38.0	41.0	32.0	41.0
52-53	37.347669007587974	40.0	37.0	41.0	31.5	41.0
54-55	37.205637494384696	40.0	37.0	41.0	31.0	41.0
56-57	37.058895590744186	40.0	37.0	41.0	31.0	41.0
58-59	36.86997042158488	40.0	36.0	41.0	31.0	41.0
60-61	36.6598663602893	39.5	36.0	41.0	31.0	41.0
62-63	36.407222095941506	39.0	35.0	41.0	30.0	41.0
64-65	36.15145965546551	39.0	35.0	41.0	30.0	41.0
66-67	35.869673093337724	38.5	35.0	40.5	30.0	41.0
68-69	35.569435302558425	38.0	35.0	40.0	30.0	41.0
70-71	35.22985286919963	37.0	35.0	40.0	29.0	41.0
72-73	34.86928760798348	37.0	34.5	39.5	29.0	41.0
74-75	34.49737916641081	36.0	34.0	39.0	29.0	41.0
76-77	34.158475237548394	36.0	34.0	39.0	29.0	40.0
78-79	33.78120680795504	35.0	34.0	37.5	28.0	39.5
80-81	33.41010219401674	35.0	34.0	37.0	28.0	39.0
82-83	33.16637149297203	35.0	34.0	37.0	28.0	39.0
84-85	32.87793580223506	35.0	34.0	36.0	28.0	38.0
86-87	32.60471071362011	35.0	34.0	36.0	27.0	37.0
88-89	32.22224029652388	35.0	34.0	36.0	26.5	37.0
90-91	32.01433064696544	35.0	33.5	35.0	26.0	36.5
92-93	31.822506168304308	35.0	33.0	35.0	26.0	36.0
94-95	31.642585592291873	35.0	33.0	35.0	25.0	36.0
96-97	31.457144334550296	35.0	33.0	35.0	25.0	36.0
98-99	31.256480086009738	35.0	33.0	35.0	24.5	35.5
100	31.087979356514165	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0983769871779252
1101	2	0.08888449424851075
1101	3	0.1225803959205507
1101	4	0.10384824307283935
1101	5	0.120151880142636
1101	6	0.12806364104535817
1101	7	0.12052953731304683
1101	8	0.06133543793148988
1101	9	0.11302657061448684
1101	10-11	0.09714981332164285
1101	12-13	0.17014871222358607
1101	14-15	0.13283055099199714
1101	16-17	0.030251414972454427
1101	18-19	0.05982847825021764
1101	20-21	0.030007651680577396
1101	22-23	0.03226407600261183
1101	24-25	0.050542034891080334
1101	26-27	0.022208921528793724
1101	28-29	0.1103656337751886
1101	30-31	0.14465489694412526
1101	32-33	0.08726116066656431
1101	34-35	0.11762740994790022
1101	36-37	0.03237482442663264
1101	38-39	0.036405043889530475
1101	40-41	0.13446578535828735
1101	42-43	0.07216856563767493
1101	44-45	0.11331419301348689
1101	46-47	0.11873896798487493
1101	48-49	0.06619744911387926
1101	50-51	0.07151702871882293
1101	52-53	0.08285532993679823
1101	54-55	0.08491120713917866
1101	56-57	0.07456904967507683
1101	58-59	0.016330873681077662
1101	60-61	0.057951110662870065
1101	62-63	0.022427817388944504
1101	64-65	-0.05742583929025358
1101	66-67	-0.07629722310821307
1101	68-69	0.04144917569419704
1101	70-71	0.009546179624706497
1101	72-73	0.012939813233693087
1101	74-75	-0.08124366346233103
1101	76-77	-0.04067214103877603
1101	78-79	-0.06902885678600512
1101	80-81	0.0029311990717317826
1101	82-83	-0.020513087488140513
1101	84-85	-0.03739946603577238
1101	86-87	-0.020496705460217868
1101	88-89	0.07908431825521234
1101	90-91	0.13499419567398974
1101	92-93	0.10696430451557859
1101	94-95	0.14970611477754403
1101	96-97	0.11450365053559963
1101	98-99	0.21957044199816877
1101	100	0.24321779011312117
1102	1	0.13389688779464137
1102	2	0.1358630557025009
1102	3	0.14604100889235028
1102	4	0.19504436571187256
1102	5	0.10210450555708661
1102	6	0.11876502900625496
1102	7	0.06729348344546082
1102	8	0.1375005013466435
1102	9	0.14690510426432724
1102	10-11	0.11214181556986347
1102	12-13	0.13037643874138638
1102	14-15	0.11261734860439532
1102	16-17	0.16448546774631723
1102	18-19	0.1257510364814749
1102	20-21	0.15688045476628076
1102	22-23	0.12649599379167853
1102	24-25	0.18379810489052772
1102	26-27	0.21078129199437257
1102	28-29	0.14059774584266904
1102	30-31	0.1521729913948704
1102	32-33	0.17112190926963677
1102	34-35	0.1992119452815686
1102	36-37	0.2082911763973172
1102	38-39	0.1376978330303018
1102	40-41	0.16160811079568305
1102	42-43	0.18014519482652958
1102	44-45	0.14841858974457267
1102	46-47	0.16146200734093696
1102	48-49	0.12050398228426218
1102	50-51	0.13520642401294936
1102	52-53	0.09405994186759159
1102	54-55	0.1693429083389617
1102	56-57	0.12320982146948722
1102	58-59	0.09371199535066665
1102	60-61	0.0936474656957742
1102	62-63	0.1931388777140839
1102	64-65	0.2571029725504488
1102	66-67	0.15528535126009757
1102	68-69	0.1420010835511647
1102	70-71	0.1724801617129117
1102	72-73	0.19916678471489035
1102	74-75	0.14255728738510243
1102	76-77	0.14012113827840267
1102	78-79	0.16322054320404789
1102	80-81	0.21301678806715785
1102	82-83	0.12316246157242006
1102	84-85	0.200898635503016
1102	86-87	0.2420853547715467
1102	88-89	0.34731895853948913
1102	90-91	0.2757165438871496
1102	92-93	0.28014783697780743
1102	94-95	0.30633361278443516
1102	96-97	0.33009695489079505
1102	98-99	0.3042765809495762
1102	100	0.28935631816057494
1103	1	0.043373867517399134
1103	2	-0.001735228517276255
1103	3	0.04179842399634737
1103	4	0.11841336162951421
1103	5	0.021975646325515186
1103	6	0.058743596814736065
1103	7	0.0448215620512471
1103	8	0.01329055924971101
1103	9	0.08871562491496832
1103	10-11	0.12525510119410654
1103	12-13	0.10013359077514394
1103	14-15	0.11893015568179521
1103	16-17	0.07439834581251148
1103	18-19	0.07012498580078841
1103	20-21	0.13270002021340588
1103	22-23	0.19783503133114522
1103	24-25	0.19187232784808117
1103	26-27	0.1781834257249173
1103	28-29	0.20561562984927662
1103	30-31	0.14915973270520055
1103	32-33	0.1264961103632416
1103	34-35	0.1507741381727996
1103	36-37	0.16323462508243125
1103	38-39	0.11481988481748573
1103	40-41	0.09427166931763509
1103	42-43	0.08029912103683756
1103	44-45	0.10112970674631327
1103	46-47	0.152236538326612
1103	48-49	0.0918371710293755
1103	50-51	0.07526933495037014
1103	52-53	0.05522507692830203
1103	54-55	0.035995542919593504
1103	56-57	0.07541513071883799
1103	58-59	0.03599431037878986
1103	60-61	0.03568403095238182
1103	62-63	0.03929964626072291
1103	64-65	0.06681947671852839
1103	66-67	0.1191581266786983
1103	68-69	0.05234069768637539
1103	70-71	0.1271641799701797
1103	72-73	0.11298228964282231
1103	74-75	0.10322728309515128
1103	76-77	0.09259510298532803
1103	78-79	-0.0074873767914667155
1103	80-81	0.0210931196029307
1103	82-83	0.024243220871440485
1103	84-85	8.605179312013433E-4
1103	86-87	-0.008692936511970117
1103	88-89	0.08555277827663588
1103	90-91	0.10724980407290019
1103	92-93	0.09056588269227817
1103	94-95	0.09457851510831716
1103	96-97	0.0927809314785506
1103	98-99	0.12034982090803936
1103	100	0.1828311973377872
1104	1	0.16485594117621716
1104	2	0.11183835335507908
1104	3	0.13869709623971715
1104	4	0.17176652924823088
1104	5	0.12202163145455813
1104	6	0.10174736883816138
1104	7	0.11970311744523343
1104	8	0.1686824618460392
1104	9	0.1746279453837971
1104	10-11	0.21755379836741184
1104	12-13	0.19452876021644983
1104	14-15	0.10960701803382022
1104	16-17	0.23685269424015587
1104	18-19	0.16471023685656405
1104	20-21	0.1712321222565265
1104	22-23	0.07756152947583672
1104	24-25	0.1402334587898224
1104	26-27	0.21153717455472787
1104	28-29	0.19415023585212765
1104	30-31	0.21319091778730836
1104	32-33	0.18021902327625838
1104	34-35	0.15818457957520593
1104	36-37	0.23718596857562346
1104	38-39	0.18732094214647077
1104	40-41	0.13716148375777237
1104	42-43	0.17193682767291563
1104	44-45	0.06375490930202687
1104	46-47	0.03640116801290816
1104	48-49	0.06345295979313192
1104	50-51	0.06406667395398102
1104	52-53	0.05527872015315438
1104	54-55	-0.03331808333680897
1104	56-57	0.04441720589831277
1104	58-59	0.06994453165937387
1104	60-61	0.07736554774517401
1104	62-63	0.07893129733521675
1104	64-65	-0.0692748468961426
1104	66-67	-0.05130880149641115
1104	68-69	-0.05059206057697452
1104	70-71	-0.015592869773080054
1104	72-73	0.05409372608591667
1104	74-75	0.054968485514891086
1104	76-77	0.00890463554173948
1104	78-79	-0.0069652920494363
1104	80-81	0.06800864331250267
1104	82-83	0.08378043793769763
1104	84-85	0.1489011968588656
1104	86-87	0.15943059606710364
1104	88-89	0.30512528107028913
1104	90-91	0.2669030988635299
1104	92-93	0.2238381340298865
1104	94-95	0.3164708360669728
1104	96-97	0.3007530640517011
1104	98-99	0.29762806785841533
1104	100	0.36097203099919284
1105	1	0.016815856498421766
1105	2	-7.937107790674247E-4
1105	3	0.023792592926067613
1105	4	0.005478054859516135
1105	5	-0.0432230656995074
1105	6	-0.0066312717030712065
1105	7	-0.11071663595978265
1105	8	-0.03353415947459126
1105	9	-0.0865698853181982
1105	10-11	-4.7127211876585307E-4
1105	12-13	-0.08018393372282873
1105	14-15	-0.05491555349206578
1105	16-17	0.018521772472496423
1105	18-19	0.015336323987625633
1105	20-21	0.02942041219064606
1105	22-23	0.11616399341854589
1105	24-25	0.09748614248547227
1105	26-27	0.11831483895247885
1105	28-29	0.12283599170084614
1105	30-31	0.15354313779568685
1105	32-33	0.11342229813106286
1105	34-35	0.12225293805345672
1105	36-37	0.08238913924984814
1105	38-39	0.07570183117250906
1105	40-41	0.05489095375931896
1105	42-43	0.035267242277022604
1105	44-45	0.07024394447385163
1105	46-47	0.1630916982479178
1105	48-49	0.10395337430525586
1105	50-51	0.14371121383358343
1105	52-53	0.0911242215288226
1105	54-55	0.036543128281088855
1105	56-57	0.10615310785672705
1105	58-59	0.06624513496297624
1105	60-61	0.013086032409546533
1105	62-63	0.09990086740270243
1105	64-65	0.12495045391037962
1105	66-67	0.14704656686860318
1105	68-69	0.044926638704438915
1105	70-71	0.1185582214768175
1105	72-73	0.11879351811899852
1105	74-75	0.06681194915640276
1105	76-77	0.148923561284505
1105	78-79	0.11439682499578652
1105	80-81	0.1252423188289029
1105	82-83	0.12755047433280708
1105	84-85	0.10857924537476293
1105	86-87	0.16150835373755257
1105	88-89	0.18618870805504173
1105	90-91	0.16646978167101878
1105	92-93	0.1152957256589282
1105	94-95	0.11548717772483741
1105	96-97	0.1720202596738254
1105	98-99	0.1373386990936467
1105	100	0.17111908931320485
1106	1	0.002665015947844296
1106	2	-0.0022107525629344593
1106	3	-0.023506820063502687
1106	4	0.03183707538325109
1106	5	-0.04842839834731194
1106	6	-0.005866901317219231
1106	7	-0.029054116672156738
1106	8	-0.03266448268477262
1106	9	-6.213668508792125E-4
1106	10-11	-0.006980748953125726
1106	12-13	-0.04039249633778752
1106	14-15	0.049473200765703496
1106	16-17	-0.050090286840188014
1106	18-19	-0.05110451133587901
1106	20-21	-0.05670390233316169
1106	22-23	-0.05469382056205774
1106	24-25	-0.06466614233244172
1106	26-27	-0.027558959341639877
1106	28-29	0.008260169891386226
1106	30-31	-0.002208087268805059
1106	32-33	0.009443325622477516
1106	34-35	-0.022352588723421718
1106	36-37	-0.0023847419309674933
1106	38-39	-0.017842446774295695
1106	40-41	0.022035526952180362
1106	42-43	-0.06177263751499851
1106	44-45	-0.02668369410223903
1106	46-47	-0.022160930388281486
1106	48-49	-0.0035327009918475483
1106	50-51	-0.042142232071633146
1106	52-53	-0.14636897350856515
1106	54-55	-0.1021581338318569
1106	56-57	-0.14171739117919202
1106	58-59	-0.11605021017434325
1106	60-61	-0.18216445471105658
1106	62-63	-0.1150134074768161
1106	64-65	-0.06223262090191639
1106	66-67	-0.09995836489569854
1106	68-69	-0.22676802158166964
1106	70-71	-0.17419067063703153
1106	72-73	-0.11409036710170284
1106	74-75	-0.13843591129715094
1106	76-77	-0.19669596627842623
1106	78-79	-0.06863389559338628
1106	80-81	-0.061992410344146265
1106	82-83	-0.13984226239056596
1106	84-85	-0.14150930903485914
1106	86-87	-0.0786601091120005
1106	88-89	0.02867992196220115
1106	90-91	0.09268275448393837
1106	92-93	0.03202304577019177
1106	94-95	0.01731135860939048
1106	96-97	0.050010718970700196
1106	98-99	0.059973023547641446
1106	100	0.06786556092082918
1107	1	0.053809936587438045
1107	2	0.04578998715438587
1107	3	0.027271027325234343
1107	4	-0.004922626320173151
1107	5	-0.025921308251831476
1107	6	-0.012433694858366096
1107	7	0.03488926808183379
1107	8	0.03814051206746427
1107	9	0.002717574257758315
1107	10-11	-0.015276271265697972
1107	12-13	-0.013240900109842357
1107	14-15	-0.01855900096870755
1107	16-17	-0.03686284238820292
1107	18-19	0.025758885147418198
1107	20-21	0.010955354944556461
1107	22-23	-0.016553921250782366
1107	24-25	0.01250515110839956
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2216	8	0.04840937332657802
2216	9	0.010149871510847674
2216	10-11	0.03832193576005949
2216	12-13	-0.0022374956025288384
2216	14-15	0.04310584409213902
2216	16-17	0.03583863016609001
2216	18-19	4.6164248551860965E-4
2216	20-21	-5.790353324144348E-4
2216	22-23	-0.022156795984848543
2216	24-25	-0.03788495222478616
2216	26-27	-0.05177132210823743
2216	28-29	-0.06416012017928097
2216	30-31	-0.07987474816394524
2216	32-33	0.030976974341555774
2216	34-35	0.0015502329945462634
2216	36-37	0.024668628720597496
2216	38-39	0.010721317389226215
2216	40-41	0.01871851404646918
2216	42-43	6.651549278799962E-4
2216	44-45	0.04188700077632035
2216	46-47	0.09415909891483665
2216	48-49	0.14553012830551637
2216	50-51	0.11408935135776943
2216	52-53	0.14306605128178518
2216	54-55	0.096176324137204
2216	56-57	0.007347218351100082
2216	58-59	-0.025800692239471346
2216	60-61	0.023357364482151866
2216	62-63	0.1004897875535562
2216	64-65	0.19336144404537237
2216	66-67	0.11085359934259031
2216	68-69	0.10735002054254039
2216	70-71	0.08543084208785956
2216	72-73	0.14370977224420045
2216	74-75	0.19701593986464871
2216	76-77	0.07943886514936338
2216	78-79	0.07835715715101088
2216	80-81	0.0691717376180847
2216	82-83	0.17488160519229723
2216	84-85	0.16001327785330943
2216	86-87	0.11468745307848138
2216	88-89	0.14187293554781633
2216	90-91	0.13582155388021278
2216	92-93	0.20832273164443293
2216	94-95	0.22866775709575293
2216	96-97	0.2294194719126601
2216	98-99	0.18411590845655823
2216	100	0.16455064527582408
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	31542.0
3	5525.0
4	3425.0
5	2831.0
6	2458.0
7	2673.0
8	3062.0
9	3348.0
10	3750.0
11	4156.0
12	4566.0
13	4966.0
14	5656.0
15	5737.0
16	5683.0
17	6022.0
18	5891.0
19	5859.0
20	6024.0
21	6324.0
22	7280.0
23	9790.0
24	14699.0
25	20120.0
26	21610.0
27	18917.0
28	19912.0
29	24291.0
30	30045.0
31	36743.0
32	47542.0
33	64507.0
34	89498.0
35	135049.0
36	247811.0
37	532299.0
38	916589.0
39	427670.0
40	931.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.75385659685592	20.069652214516715	13.410573358928685	23.76591782969868
2	15.052168026308173	20.026726653635297	41.24231123357132	23.67879408648521
3	17.863908404144006	25.637044521611145	30.19621215605561	26.302834918189237
4	11.79735785682853	16.748709014001022	39.96210114145614	31.491831987714303
5	12.358771929194308	38.494793466632274	36.399498781801235	12.74693582237218
6	30.46671055197446	38.981070779962586	17.931924622135647	12.620294045927302
7	26.865802961439233	32.84269293311192	22.937363893884633	17.354140211564207
8	24.451527634617527	37.50564759712251	20.524966150332034	17.517858617927935
9	24.433042379550127	17.14888996875686	21.505977643859783	36.91209000783323
10-11	23.299640201530245	27.363629914903665	29.553686475187195	19.783043408378894
12-13	23.94843991246831	24.672375692393555	29.463848643298306	21.91533575183983
14-15	21.251791535565744	26.7918184043342	26.35784523323589	25.598544826864174
16-17	20.97404003736735	30.184279495116996	26.975658427101884	21.86602204041377
18-19	20.843889736912992	29.31843404866661	28.858435844482354	20.979240369938044
20-21	22.083979633747237	28.56303914115632	28.319263335159768	21.03371788993668
22-23	21.647633251118076	28.332981666326972	28.37773166700038	21.641653415554575
24-25	21.560490130650262	28.45197361851732	28.269902308631163	21.717633942201246
26-27	21.316503687613213	28.368519604985682	28.427224748033897	21.887751959367204
28-29	21.32876815157189	28.411396480864052	28.511333160696385	21.74850220686767
30-31	21.50166805514127	28.93011192859212	28.161263947076222	21.406956069190393
32-33	21.191979716180253	28.547899475986956	28.66599906396738	21.594121743865415
34-35	21.369234065087706	28.35351405689199	28.650545911183507	21.626705966836802
36-37	21.71343027584119	28.72732522498354	28.08457923166401	21.47466526751126
38-39	21.501035579621114	28.84336153418777	28.29319527041611	21.362407615775002
40-41	21.233497436460986	28.536975456715396	28.56886594306148	21.66066116376214
42-43	21.230421159422892	28.41650880295916	28.6017974017579	21.75127263586005
44-45	21.343502754657205	28.572965107196584	28.54407361745513	21.53945852069108
46-47	21.392242435777845	28.771184804848858	28.407035550340314	21.429537209032983
48-49	21.373100743695268	28.61559632352136	28.386197960947335	21.625104971836038
50-51	21.345765690092584	28.49034274211287	28.441718434735424	21.722173133059126
52-53	21.392908330164108	28.461361111919132	28.445990811576998	21.69973974633977
54-55	21.53289736987306	28.88884260445464	28.21882753827279	21.359432487399513
56-57	21.381673979481267	28.57087965824973	28.305197984647307	21.742248377621692
58-59	21.20145479408372	28.239531098952714	28.708697881729034	21.85031622523453
60-61	21.52034420800862	28.67562761887542	28.228310185812784	21.57571798730317
62-63	21.311167230604735	28.67923579733421	28.30817681770152	21.70142015435953
64-65	21.334262922240416	28.418883103596436	28.444127798484853	21.8027261756783
66-67	21.158658065554036	28.408305790525272	28.3763460784356	22.05669006548509
68-69	21.594384219046482	28.514262095633175	28.308083673642344	21.583270011677996
70-71	21.531377878836466	28.338457389476684	28.386307449473296	21.74385728221355
72-73	21.263687696707194	28.501632729204673	28.471935317817064	21.762744256271063
74-75	21.3543583734657	28.521931826383977	28.382008680872882	21.741701119277433
76-77	21.3190266114364	28.53402784136919	28.262473044721077	21.884472502473333
78-79	21.498485207861297	28.590653482028888	28.313227377185846	21.597633932923966
80-81	21.399791791380565	28.345926519378878	28.445416340031464	21.808865349209093
82-83	21.23550196329591	28.4125160406007	28.580341975641808	21.771640020461586
84-85	21.21825043280786	28.364239459364537	28.452488811274947	21.965021296552656
86-87	21.411461539994626	28.43786858369239	28.34913468287147	21.80153519344152
88-89	21.190113477045273	28.3436455690806	28.402446177572056	22.063794776302075
90-91	21.179760431678982	28.251381787915136	28.565851116857534	22.003006663548348
92-93	21.33602035682251	28.425661375422408	28.3843081923203	21.854010075434783
94-95	21.112128888006588	28.17307200772539	28.549424669045386	22.165374435222635
96-97	21.1697358032376	28.15155110809176	28.58885949214787	22.089853596522772
98-99	21.152104156111342	28.226136385188315	28.5026746178182	22.11908484088214
100	21.28685007954892	28.180800749103717	28.382852114151614	22.149497057195745
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	360.0
1	351.0
2	320.0
3	267.0
4	196.0
5	159.0
6	169.5
7	177.0
8	182.5
9	183.0
10	183.5
11	197.5
12	218.5
13	241.5
14	294.5
15	365.5
16	446.5
17	583.0
18	870.0
19	1318.0
20	1965.0
21	2947.5
22	4550.0
23	7138.5
24	10466.0
25	14332.0
26	19639.5
27	26359.0
28	34699.5
29	43923.5
30	52307.5
31	61195.5
32	69985.5
33	77810.5
34	84682.0
35	90222.0
36	94928.0
37	99880.0
38	103465.5
39	104257.0
40	104660.0
41	104642.5
42	102658.5
43	100537.5
44	98334.0
45	95149.0
46	93046.0
47	90285.5
48	86701.5
49	84055.0
50	81130.5
51	77657.5
52	72483.0
53	67659.5
54	64461.5
55	61110.0
56	56428.5
57	50966.0
58	46280.5
59	41987.5
60	37381.0
61	31669.0
62	26669.0
63	22306.0
64	17911.0
65	14285.0
66	11161.0
67	8293.5
68	6138.5
69	4742.0
70	3452.0
71	2423.0
72	1834.0
73	1357.0
74	1007.0
75	736.0
76	492.0
77	349.0
78	219.5
79	142.5
80	101.5
81	59.5
82	38.5
83	29.5
84	18.0
85	17.5
86	16.0
87	8.5
88	6.5
89	6.5
90	7.0
91	5.5
92	5.0
93	4.5
94	1.5
95	0.5
96	1.5
97	2.0
98	2.0
99	2.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3338479122924762
2	0.11878766202683781
3	0.06438521100789608
4	0.03863830844645632
5	0.042480593765945936
6	0.00969548632020744
7	0.007505024596012427
8	0.015476868903738542
9	0.018313696382614052
10-11	0.021671207386093298
12-13	0.01572823336389207
14-15	0.018942107532997868
16-17	0.01982188314353521
18-19	0.01978597393494185
20-21	0.024615762490748892
22-23	0.015925734011155556
24-25	0.026105994647373364
26-27	0.019660291704865086
28-29	0.01931915422322816
30-31	0.020486203502512387
32-33	0.025495538101286234
34-35	0.015063913004914893
36-37	0.009480031068647275
38-39	0.01328640717954353
40-41	0.009749350133097481
42-43	0.00811548114209956
44-45	0.008761846896780057
46-47	0.008205254163582964
48-49	0.004506605678466792
50-51	0.006661158194068445
52-53	0.007540933804605787
54-55	0.003142055751919078
56-57	0.002782963665985469
58-59	0.0027650090616887888
60-61	0.0032138741691058
62-63	0.002459780788645221
64-65	0.002064779494118251
66-67	0.0016159143867012402
68-69	0.0027650090616887888
70-71	0.0021725071198983336
72-73	0.0016697781995912815
74-75	0.0020827340984149313
76-77	0.0034472840249626453
78-79	0.003429329420665965
80-81	0.0033754656077759236
82-83	0.002531599205831943
84-85	0.0028009182702821494
86-87	0.002531599205831943
88-89	0.6094690428508177
90-91	0.6205649883061662
92-93	0.6168663398210501
94-95	0.6027540208438592
96-97	0.6008149235798177
98-99	0.5978344592665688
100	0.5994324190489733
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2784801.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.84543897910879
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.7652978615517	53.43866209737234
2	13.284677524319122	18.026095566666616
3	3.8992582139193708	7.936406556487663
4	1.576708329534485	4.278898750371378
5	0.7665509893799628	2.6003494187176868
6	0.44951701096056357	1.8298607362317774
7	0.2885740620135123	1.3704903740703913
8	0.19317677790725193	1.048493063814985
9	0.13685074328687463	0.83562288776238
>10	0.6104234520476142	7.001557512466619
>50	0.02362068370626069	1.0896766432630636
>100	0.005291370232816446	0.5223192204441648
>500	5.29811403082469E-5	0.021567172330960598
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.590920859336089E-5	0.0	0.0	0.0	0.0
8	3.590920859336089E-5	0.0	0.0	0.0	0.0
9	3.590920859336089E-5	0.0	0.0	0.0	0.0
10-11	3.590920859336089E-5	0.0	0.0	0.0	0.0
12-13	3.590920859336089E-5	0.0	0.0	0.0	0.0
14-15	3.590920859336089E-5	0.0	0.0	0.0	0.0
16-17	3.590920859336089E-5	0.0	0.0	0.0	0.0
18-19	3.590920859336089E-5	0.0	0.0	1.7954604296680446E-5	0.0
20-21	3.590920859336089E-5	0.0	0.0	3.590920859336089E-5	0.0
22-23	3.590920859336089E-5	0.0	0.0	1.0772762578008268E-4	0.0
24-25	3.590920859336089E-5	0.0	0.0	1.975006472634849E-4	0.0
26-27	3.590920859336089E-5	0.0	0.0	2.8727366874688713E-4	0.0
28-29	3.590920859336089E-5	0.0	0.0	0.0011850038835809094	0.0
30-31	3.590920859336089E-5	0.0	0.0	0.004129558988236502	0.0
32-33	5.386381289004134E-5	0.0	0.0	0.011185718476831917	0.0
34-35	7.181841718672178E-5	0.0	0.0	0.021473706738829812	0.0
36-37	7.181841718672178E-5	0.0	0.0	0.03973353930855382	0.0
38-39	7.181841718672178E-5	0.0	0.0	0.06971772848401017	0.0
40-41	1.0772762578008267E-4	0.0	0.0	0.10819444549179635	0.0
42-43	1.0772762578008267E-4	0.0	0.0	0.14778434796597673	0.0
44-45	1.0772762578008267E-4	0.0	0.0	0.18916971086982518	0.0
46-47	1.0772762578008267E-4	0.0	0.0	0.24159715541613205	0.0
48-49	1.0772762578008267E-4	0.0	0.0	0.3032532665709327	0.0
50-51	1.0772762578008267E-4	0.0	0.0	0.3718757641928454	0.0
52-53	1.0772762578008267E-4	0.0	0.0	0.43918757570110034	0.0
54-55	1.0772762578008267E-4	0.0	0.0	0.5147764597901251	0.0
56-57	1.0772762578008267E-4	0.0	0.0	0.5990374177544464	0.0
58-59	1.0772762578008267E-4	0.0	0.0	0.6986136531838361	0.0
60-61	1.0772762578008267E-4	0.0	0.0	0.8089626511912341	0.0
62-63	1.0772762578008267E-4	0.0	0.0	0.9182343729408313	0.0
64-65	1.0772762578008267E-4	0.0	0.0	1.034382708136057	0.0
66-67	1.0772762578008267E-4	0.0	0.0	1.1675340536002392	0.0
68-69	1.0772762578008267E-4	0.0	0.0	1.3193940967415625	0.0
70-71	1.0772762578008267E-4	0.0	0.0	1.48556395950734	0.0
72-73	1.0772762578008267E-4	0.0	0.0	1.661088171111688	0.0
74-75	1.0772762578008267E-4	0.0	0.0	1.8381564786855504	0.0
76-77	1.2568223007676312E-4	0.0	0.0	2.0282418743745065	1.7954604296680446E-5
78-79	1.7954604296680445E-4	0.0	0.0	2.2448282660053627	3.590920859336089E-5
80-81	2.1545525156016533E-4	0.0	0.0	2.4778251659633845	3.590920859336089E-5
82-83	2.1545525156016533E-4	0.0	0.0	2.725670523674762	3.590920859336089E-5
84-85	2.1545525156016533E-4	0.0	0.0	2.978166123898979	3.590920859336089E-5
86-87	2.1545525156016533E-4	0.0	0.0	3.237628110590308	3.590920859336089E-5
88	2.1545525156016533E-4	0.0	0.0	3.451018582656355	3.590920859336089E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3175	0.0	33.53771	1
TCAACGC	3075	0.0	31.777597	4
GGTATCA	2020	0.0	31.021936	1
ATCAACG	3150	0.0	30.72878	3
CAACGCA	3230	0.0	30.397018	5
AACGCAG	3510	0.0	28.095425	6
GTACATG	7260	0.0	27.646595	1
TACATGG	7245	0.0	25.631945	2
TATCAAC	4125	0.0	24.617994	2
TACACCG	290	0.0	24.298557	5
ACATGGG	7465	0.0	24.170748	3
ACGCAGA	4310	0.0	22.008858	7
CGCAGAG	4285	0.0	21.91887	8
TATACCG	245	8.554707E-9	21.09181	5
CATGGGG	4760	0.0	20.725985	4
GTACTAG	670	0.0	18.28383	1
GCAGAGT	5460	0.0	17.02988	9
CCTACAC	1115	0.0	16.43533	3
GTATAGA	1150	0.0	16.388184	1
TAGACTG	1360	0.0	15.889351	5
>>END_MODULE