FastQCFastQC Report
Wed 25 May 2016
SRR1033176_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033176_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2784801
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46360.16647509103882108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39480.1417695555265888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37900.052.5215761
GTATCAA68300.041.9597741
TCAACGC80600.035.0528534
ATCAACG81000.034.8217123
CAACGCA83000.033.869975
AACGCAG86550.032.47146
TATCAAC87450.032.4146542
ACGCAGA98950.028.3547237
CGCAGAG99450.027.9738738
GTACATG74850.025.3995571
GCAGAGT111150.024.479639
TACATGG74200.024.0114972
ACATGGG75200.022.8795343
TGGTATC19300.021.677872
GTGGTAT20300.021.0951161
GAGTACT77400.020.82481212-13
CAGAGTA110600.020.69233710-11
AGAGTAC104350.020.2429110-11
CATGGGG48300.018.784264
GTACTTT86650.018.68308614-15
Produced by FastQC (version 0.11.5)