##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033176_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2784801 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.78552435165026 34.0 31.0 34.0 31.0 34.0 2 32.87198474864093 34.0 31.0 34.0 31.0 34.0 3 32.96430157846108 34.0 31.0 34.0 31.0 34.0 4 36.384505391947215 37.0 37.0 37.0 35.0 37.0 5 36.32286651721254 37.0 37.0 37.0 35.0 37.0 6 36.41003037559955 37.0 37.0 37.0 35.0 37.0 7 36.38501925272219 37.0 37.0 37.0 35.0 37.0 8 36.37717201336828 37.0 37.0 37.0 35.0 37.0 9 38.19778073909051 39.0 39.0 39.0 37.0 39.0 10-11 38.182813421856714 39.0 39.0 39.0 37.0 39.0 12-13 38.147686854464645 39.0 39.0 39.0 37.0 39.0 14-15 39.69477334287082 41.0 40.0 41.0 37.0 41.0 16-17 39.71212952020629 41.0 40.0 41.0 37.0 41.0 18-19 39.727636552845254 41.0 40.0 41.0 37.0 41.0 20-21 39.72381976306386 41.0 40.0 41.0 37.0 41.0 22-23 39.65827306870401 41.0 40.0 41.0 37.0 41.0 24-25 39.61787395221418 41.0 40.0 41.0 37.0 41.0 26-27 39.52447122792616 41.0 40.0 41.0 37.0 41.0 28-29 39.437932189768674 41.0 39.0 41.0 36.5 41.0 30-31 39.35033831860876 41.0 39.0 41.0 36.0 41.0 32-33 39.389143963967264 41.0 39.0 41.0 36.0 41.0 34-35 39.33950522856031 41.0 39.0 41.0 36.0 41.0 36-37 39.261319031413734 41.0 39.0 41.0 35.0 41.0 38-39 39.17606482473972 41.0 39.0 41.0 35.0 41.0 40-41 39.07236513488756 41.0 39.0 41.0 35.0 41.0 42-43 38.966954909884045 41.0 39.0 41.0 35.0 41.0 44-45 38.83639064335297 41.0 39.0 41.0 35.0 41.0 46-47 38.64700727269202 41.0 39.0 41.0 35.0 41.0 48-49 38.47819736491046 41.0 39.0 41.0 35.0 41.0 50-51 38.36012645068714 41.0 38.5 41.0 35.0 41.0 52-53 38.235444830707834 40.5 38.0 41.0 34.5 41.0 54-55 38.109430440451575 40.0 38.0 41.0 34.0 41.0 56-57 37.96536520921962 40.0 37.5 41.0 34.0 41.0 58-59 37.7894738259574 40.0 37.0 41.0 34.0 41.0 60-61 37.61027448639957 40.0 37.0 41.0 34.0 41.0 62-63 37.38829399299986 40.0 36.0 41.0 33.0 41.0 64-65 37.14625694977846 39.0 36.0 41.0 33.0 41.0 66-67 36.8760442846724 39.0 35.0 41.0 33.0 41.0 68-69 36.564150723875784 38.5 35.0 41.0 32.5 41.0 70-71 36.23528970292671 37.5 35.0 40.0 32.0 41.0 72-73 35.88677754712096 37.0 35.0 40.0 32.0 41.0 74-75 35.54777432929678 36.5 35.0 39.0 32.0 41.0 76-77 35.20398531169732 36.0 35.0 39.0 31.0 41.0 78-79 34.856112519350575 36.0 35.0 38.5 31.0 40.0 80-81 34.50620008395573 35.0 35.0 37.0 31.0 39.0 82-83 34.17095314889646 35.0 35.0 37.0 31.0 39.0 84-85 33.88699354101065 35.0 34.0 36.5 31.0 38.0 86-87 33.640542358322904 35.0 34.0 36.0 31.0 37.0 88-89 33.412350290020726 35.0 34.0 36.0 30.5 37.0 90-91 33.20614471195608 35.0 34.0 35.5 30.0 36.5 92-93 33.03967464820646 35.0 34.0 35.0 30.0 36.0 94-95 32.889402689815185 35.0 34.0 35.0 30.0 36.0 96-97 32.76412892698616 35.0 34.0 35.0 30.0 36.0 98-99 32.661196437375594 35.0 34.0 35.0 30.0 36.0 100 32.56490535589437 35.0 34.0 35.0 30.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.055668500374295604 1101 2 0.06269397477738892 1101 3 0.088441745383669 1101 4 0.05900569363262065 1101 5 0.062222499213596905 1101 6 0.08616847369671632 1101 7 0.10241779302734244 1101 8 0.1492496784841464 1101 9 0.19136389075438132 1101 10-11 0.17694605188071932 1101 12-13 0.16126848915526892 1101 14-15 0.18762007567796246 1101 16-17 0.20801772844514943 1101 18-19 0.27470351526739023 1101 20-21 0.18343901993345924 1101 22-23 0.2206284409949646 1101 24-25 0.21940474650273245 1101 26-27 0.18837079058292971 1101 28-29 0.17909415503739012 1101 30-31 0.15828430831931684 1101 32-33 0.12812363490581902 1101 34-35 0.1358154425960194 1101 36-37 0.12708718429483667 1101 38-39 0.07406469015262473 1101 40-41 0.13769083394333137 1101 42-43 0.040314838734900604 1101 44-45 0.017212640257255885 1101 46-47 0.09961616681503926 1101 48-49 0.10900874248910952 1101 50-51 0.06718332460453524 1101 52-53 0.14050492957355942 1101 54-55 0.13363422948361148 1101 56-57 0.14112222966195986 1101 58-59 0.17477181652061802 1101 60-61 0.15695165962725355 1101 62-63 0.1705148837082362 1101 64-65 0.13849219812464497 1101 66-67 0.14698221279631696 1101 68-69 0.17944427831417187 1101 70-71 0.18575800416707722 1101 72-73 0.2320397587673284 1101 74-75 0.23569409163692967 1101 76-77 0.1764116832590119 1101 78-79 0.10510075783432882 1101 80-81 0.09895096332459019 1101 82-83 0.15833649283904805 1101 84-85 0.14006265921099015 1101 86-87 0.11408410899895216 1101 88-89 0.15229660540993706 1101 90-91 0.08131362498982497 1101 92-93 0.06072436049000629 1101 94-95 0.06308237538529937 1101 96-97 0.052267647376517345 1101 98-99 0.1422807126153458 1101 100 0.0886587527099536 1102 1 0.06426850227033043 1102 2 0.06310662162739789 1102 3 0.07913404277666558 1102 4 0.03190761978110146 1102 5 0.05726956822620366 1102 6 0.06471272054251642 1102 7 0.0796375441301933 1102 8 0.09695352440473926 1102 9 0.14388697138161177 1102 10-11 0.10752248868718794 1102 12-13 0.17048424716123378 1102 14-15 0.1823621236712185 1102 16-17 0.1514263784321841 1102 18-19 0.16290930208915455 1102 20-21 0.17091786650661334 1102 22-23 0.15876319801512295 1102 24-25 0.16963758841295373 1102 26-27 0.25015145881499734 1102 28-29 0.1762022830570018 1102 30-31 0.17246598383824363 1102 32-33 0.20038952648469177 1102 34-35 0.21742851581963407 1102 36-37 0.19093430094201835 1102 38-39 0.1916759252634037 1102 40-41 0.18528563850195923 1102 42-43 0.16409749806106078 1102 44-45 0.15411896286411064 1102 46-47 0.20839075306758303 1102 48-49 0.18378924748054715 1102 50-51 0.13283228920833068 1102 52-53 0.16579154543953223 1102 54-55 0.16276008437035472 1102 56-57 0.1453218772336271 1102 58-59 0.12258095069726949 1102 60-61 0.1527736670869757 1102 62-63 0.1515434528957087 1102 64-65 0.10260675927943197 1102 66-67 0.058883311573254105 1102 68-69 0.12002417083508732 1102 70-71 0.10752568067896817 1102 72-73 0.0661846200835754 1102 74-75 0.09074962916285756 1102 76-77 0.049157206186791313 1102 78-79 0.07645228762756062 1102 80-81 0.0895945287803741 1102 82-83 0.0463029704608644 1102 84-85 0.11959407679804457 1102 86-87 0.13098039042423437 1102 88-89 0.11112201443030045 1102 90-91 0.052848492057805174 1102 92-93 0.14149038758326782 1102 94-95 0.10106851460682975 1102 96-97 0.053309166727281365 1102 98-99 0.07586141978102034 1102 100 0.11673925722089251 1103 1 0.06044431953893081 1103 2 0.08935088987168172 1103 3 0.09347475939705419 1103 4 0.053799556653288505 1103 5 0.030030628409292603 1103 6 0.0565236553781574 1103 7 0.09252622852046244 1103 8 0.13248030623491758 1103 9 0.13243965081974096 1103 10-11 0.11499206428812414 1103 12-13 0.05325325969648276 1103 14-15 0.07137202570517331 1103 16-17 0.11986328437478733 1103 18-19 0.09972148826556548 1103 20-21 0.08382743586214758 1103 22-23 0.11453834274767871 1103 24-25 0.13674025383154742 1103 26-27 0.18174692205833765 1103 28-29 0.06893068788996715 1103 30-31 0.09065929879180601 1103 32-33 0.10329188400065448 1103 34-35 0.1508587558938146 1103 36-37 0.1355357382393052 1103 38-39 0.1290198371328728 1103 40-41 0.1647772050676437 1103 42-43 0.13454113036819138 1103 44-45 0.12349625704114686 1103 46-47 0.15905824358588205 1103 48-49 0.16076733859974723 1103 50-51 0.14449316607062457 1103 52-53 0.134118817865577 1103 54-55 0.13495333456044278 1103 56-57 0.10836684743364344 1103 58-59 0.1520162006528949 1103 60-61 0.11761990278535706 1103 62-63 0.09457192849154694 1103 64-65 0.032091761640280936 1103 66-67 0.10055308462749224 1103 68-69 0.13681485453404463 1103 70-71 0.09851954145305797 1103 72-73 0.09442538557779301 1103 74-75 0.07047445719449996 1103 76-77 0.015026416100020867 1103 78-79 0.05896320014741718 1103 80-81 0.08251146309842028 1103 82-83 0.059433200017259935 1103 84-85 0.10415620919315671 1103 86-87 0.08464927887968798 1103 88-89 0.07318649575645253 1103 90-91 0.020678742474061096 1103 92-93 0.05792988004849775 1103 94-95 0.11344210345966843 1103 96-97 0.10875686683323949 1103 98-99 0.08699521484277284 1103 100 0.07341550536741437 1104 1 0.05073078349818161 1104 2 0.0798846427005131 1104 3 0.09441182520220792 1104 4 0.08108339638963002 1104 5 0.015164601221037799 1104 6 0.04320277239057191 1104 7 0.09234473713280522 1104 8 0.11591451076346715 1104 9 0.13771770549525542 1104 10-11 0.11175400570862593 1104 12-13 0.142197290530639 1104 14-15 0.1614875258738735 1104 16-17 0.20005213776958897 1104 18-19 0.15286766797238016 1104 20-21 0.11883407226928 1104 22-23 0.10346243328940119 1104 24-25 0.11327796839987059 1104 26-27 0.08570599310024107 1104 28-29 0.13777770033701842 1104 30-31 0.09725896900020814 1104 32-33 0.09759299959696932 1104 34-35 0.16349370013227826 1104 36-37 0.10111689267074553 1104 38-39 0.13634885415138598 1104 40-41 0.19699960274981976 1104 42-43 0.15042583051627645 1104 44-45 0.17134921209743936 1104 46-47 0.21422906664292185 1104 48-49 0.18275886753949067 1104 50-51 0.19814543807595442 1104 52-53 0.1808393251495417 1104 54-55 0.1453054717174922 1104 56-57 0.25165210474018096 1104 58-59 0.22477277153670627 1104 60-61 0.2571578107251753 1104 62-63 0.20908751098020417 1104 64-65 0.26637585031043187 1104 66-67 0.26248507415691336 1104 68-69 0.27321248626334693 1104 70-71 0.25200624140594385 1104 72-73 0.31507358581561107 1104 74-75 0.25956892666025766 1104 76-77 0.24946540300887676 1104 78-79 0.23432710591518457 1104 80-81 0.20692252994245308 1104 82-83 0.19981879884927878 1104 84-85 0.15475657466161152 1104 86-87 0.18471454632635442 1104 88-89 0.1568297169526076 1104 90-91 0.18317668173347812 1104 92-93 0.18346964786379516 1104 94-95 0.16678549284149824 1104 96-97 0.11767187732245787 1104 98-99 0.12717197977798378 1104 100 0.14268051459551145 1105 1 0.06741084907938699 1105 2 0.06723828957681377 1105 3 0.07277637149513083 1105 4 0.062394807832362176 1105 5 0.008065147822684082 1105 6 0.015575739868431526 1105 7 -0.025303228281153167 1105 8 -0.031092370054054186 1105 9 0.02663795608781072 1105 10-11 0.032398322053950324 1105 12-13 0.0522646781700189 1105 14-15 0.08303392428984324 1105 16-17 0.08843102562025962 1105 18-19 0.061198991169852945 1105 20-21 0.09209971330668765 1105 22-23 0.14072183973959085 1105 24-25 0.05602404218338819 1105 26-27 0.0407709739528741 1105 28-29 0.10271740641632476 1105 30-31 0.09413603028551876 1105 32-33 0.09723547617514328 1105 34-35 0.08327957197748503 1105 36-37 -0.00844079858696034 1105 38-39 0.028974624439634056 1105 40-41 0.11362612643579695 1105 42-43 0.12937391357544925 1105 44-45 0.17885149911235487 1105 46-47 0.22300825288974835 1105 48-49 0.1197955282360823 1105 50-51 0.12284013338415889 1105 52-53 0.057441743931399 1105 54-55 0.05529684456083572 1105 56-57 0.08028942617112023 1105 58-59 0.06278658526204595 1105 60-61 0.04078439689560298 1105 62-63 0.042720034966535536 1105 64-65 -0.007827828244167279 1105 66-67 -0.010603053664070217 1105 68-69 -0.03454627377733033 1105 70-71 0.012957150961057096 1105 72-73 0.0018569634197262985 1105 74-75 -0.014229170395189783 1105 76-77 -0.05824216278409011 1105 78-79 -0.07578847122199761 1105 80-81 -0.045173925277040894 1105 82-83 -0.004076599534563741 1105 84-85 -0.03759562599211819 1105 86-87 -0.02837858264097548 1105 88-89 -2.2079426705801097E-4 1105 90-91 -0.029743270893021645 1105 92-93 -0.024226072165667745 1105 94-95 -0.01661873586309781 1105 96-97 -0.03604213691468061 1105 98-99 -0.056312266073405226 1105 100 -0.023064655612522245 1106 1 -0.0030760066837203226 1106 2 0.010758098767311708 1106 3 -0.003789146610664318 1106 4 -0.009141630295246728 1106 5 -0.011669785970127577 1106 6 0.018353260484225586 1106 7 -0.03095826778903188 1106 8 -0.0037766879385259244 1106 9 0.02144643598730056 1106 10-11 -0.016528345990920457 1106 12-13 0.012852372377572863 1106 14-15 0.05224261715370204 1106 16-17 0.062439550130854116 1106 18-19 0.011870589182258584 1106 20-21 6.33716956812691E-5 1106 22-23 -0.003638143324629084 1106 24-25 0.00924317048379919 1106 26-27 -0.01599354414732801 1106 28-29 -0.017507347700352227 1106 30-31 0.021025732300920197 1106 32-33 0.06752731246884025 1106 34-35 0.05373193632599538 1106 36-37 0.009472619800462212 1106 38-39 0.03933785904597897 1106 40-41 0.03650984727576656 1106 42-43 0.018826932136427388 1106 44-45 0.00442125812677574 1106 46-47 0.12447444907746075 1106 48-49 0.07854917871539158 1106 50-51 0.12733189385723875 1106 52-53 0.09594653112056761 1106 54-55 0.05176619765760648 1106 56-57 0.11822330425152927 1106 58-59 0.12246877567177705 1106 60-61 0.16268737550864643 1106 62-63 0.09358665244835151 1106 64-65 0.1342895279520846 1106 66-67 0.13085361186601574 1106 68-69 0.15578831634560686 1106 70-71 0.09024551619585708 1106 72-73 0.18189986450670403 1106 74-75 0.1338660793328117 1106 76-77 0.028677314973769796 1106 78-79 0.10974982600598082 1106 80-81 0.11404835867674024 1106 82-83 0.07957800870221376 1106 84-85 0.06569985373192111 1106 86-87 0.05359623149178816 1106 88-89 0.10793898517052725 1106 90-91 0.15008493640614518 1106 92-93 0.1541125203996856 1106 94-95 0.11775665903591204 1106 96-97 0.1273859535371784 1106 98-99 0.09988235888226171 1106 100 0.1594052013568401 1107 1 -7.441821865654674E-4 1107 2 -0.027799830399942493 1107 3 -0.022004467159113972 1107 4 -0.01734526140940318 1107 5 -0.11858105817741915 1107 6 -0.13371468471443393 1107 7 -0.08215348964333202 1107 8 -0.022704363257240345 1107 9 -0.0534924338367091 1107 10-11 -0.021010397223122368 1107 12-13 -0.03973399756618079 1107 14-15 -0.02283912778177921 1107 16-17 -0.020499179002676726 1107 18-19 0.02432838681811944 1107 20-21 -0.004748996308002518 1107 22-23 -0.011692427346915224 1107 24-25 -0.0013292600703493918 1107 26-27 0.027479212392556462 1107 28-29 -0.010237948321439205 1107 30-31 0.017833899033561806 1107 32-33 -0.019574046991124305 1107 34-35 0.015590944697358111 1107 36-37 -0.013313393534438944 1107 38-39 -0.07495562161620484 1107 40-41 -0.05743782973932099 1107 42-43 -0.04507142183415169 1107 44-45 -0.019968304409403004 1107 46-47 -0.08410079386620595 1107 48-49 -0.1476791690284287 1107 50-51 -0.1600627309691589 1107 52-53 -0.11858031222370613 1107 54-55 -0.21787360031392922 1107 56-57 -0.21323144347040568 1107 58-59 -0.18143565267567396 1107 60-61 -0.1858974754585745 1107 62-63 -0.19720745517669513 1107 64-65 -0.25374955319710324 1107 66-67 -0.2249840352561705 1107 68-69 -0.2192030611741913 1107 70-71 -0.1802385611758197 1107 72-73 -0.16320038483111432 1107 74-75 -0.1308995527800505 1107 76-77 -0.17682952656489448 1107 78-79 -0.1816405230533178 1107 80-81 -0.16100168720878116 1107 82-83 -0.14914835962600392 1107 84-85 -0.16127217098263458 1107 86-87 -0.17296691152471766 1107 88-89 -0.2022118327266753 1107 90-91 -0.21475206772341693 1107 92-93 -0.2760921137895096 1107 94-95 -0.34078738459299274 1107 96-97 -0.2661028627987747 1107 98-99 -0.268396701115293 1107 100 -0.3191331381907787 1108 1 0.019790433776236682 1108 2 0.03540118511882184 1108 3 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-0.08090647354538305 2214 12-13 -0.059197278881391924 2214 14-15 -0.0847954977935359 2214 16-17 -0.12226086306701234 2214 18-19 -0.10762093619914737 2214 20-21 -0.11346426778712271 2214 22-23 -0.1488718011401886 2214 24-25 -0.10086801985592331 2214 26-27 -0.13801285587772583 2214 28-29 -0.029003060241322487 2214 30-31 -0.010879611565961511 2214 32-33 -0.08370256577704538 2214 34-35 -0.08733308874187884 2214 36-37 -0.07624065574952965 2214 38-39 -0.10884912753097353 2214 40-41 -0.026386659601051576 2214 42-43 -0.027936141538717152 2214 44-45 -0.021417678152523933 2214 46-47 -0.011751956764307181 2214 48-49 -0.037392364646009923 2214 50-51 -0.037457888490664004 2214 52-53 -0.0971315969482518 2214 54-55 -0.14223180414324332 2214 56-57 -0.14106366014817695 2214 58-59 -0.2118497329986937 2214 60-61 -0.23925217704435653 2214 62-63 -0.24583501935829588 2214 64-65 -0.13986210756847584 2214 66-67 -0.2415283085415041 2214 68-69 -0.2013791706860033 2214 70-71 -0.1563158249576233 2214 72-73 -0.21974018788571925 2214 74-75 -0.19030088450360694 2214 76-77 -0.13624490162604275 2214 78-79 -0.27296246542567815 2214 80-81 -0.21814970383812238 2214 82-83 -0.1776936779183913 2214 84-85 -0.17298137477654763 2214 86-87 -0.09537317584645422 2214 88-89 -0.2242237215938161 2214 90-91 -0.13697059923821797 2214 92-93 -0.01635807712398929 2214 94-95 -0.03491683744955765 2214 96-97 -0.13596884476499582 2214 98-99 -0.2279605293509661 2214 100 -0.4050508536674968 2215 1 -0.05169544190034259 2215 2 -0.09761919019310739 2215 3 -0.07666575507981577 2215 4 -0.08387088282150046 2215 5 -0.056848082954864765 2215 6 -0.05177341179406625 2215 7 -0.04775030141465919 2215 8 -0.015540172546259612 2215 9 -0.0626122220298555 2215 10-11 -0.027931136002649737 2215 12-13 -0.04241897145244877 2215 14-15 -0.06282425280266324 2215 16-17 -0.042886522068251054 2215 18-19 -0.05702909605883377 2215 20-21 -0.09729371122683972 2215 22-23 -0.07986195529403517 2215 24-25 -0.06430361243507576 2215 26-27 -0.0504329714621079 2215 28-29 -0.038161613078308676 2215 30-31 -0.03833993470485808 2215 32-33 -0.030006756784231925 2215 34-35 -0.044732320683756654 2215 36-37 -0.030006904471704843 2215 38-39 -0.03975085143723334 2215 40-41 -0.06370120178288374 2215 42-43 0.020066550501894653 2215 44-45 0.0494538146982606 2215 46-47 -0.011725494852242946 2215 48-49 -0.026418193307947035 2215 50-51 0.09443383330535937 2215 52-53 0.02666495133486535 2215 54-55 0.013522943094677942 2215 56-57 -0.06376505690281675 2215 58-59 -0.011720127836838401 2215 60-61 0.011932866629216221 2215 62-63 -0.06093090886517416 2215 64-65 0.012404301469906898 2215 66-67 -0.05231229440126128 2215 68-69 -0.013586346234191637 2215 70-71 -0.0011638361971719746 2215 72-73 0.0832623391597167 2215 74-75 0.046244475713542954 2215 76-77 0.029830483839170086 2215 78-79 0.052007075391202306 2215 80-81 0.08895594192051703 2215 82-83 0.125117123395313 2215 84-85 0.06775750449298101 2215 86-87 0.06599586229259558 2215 88-89 0.05575958367093392 2215 90-91 0.09722309846564059 2215 92-93 0.12324138176349919 2215 94-95 0.10077702679420497 2215 96-97 0.12183006503108373 2215 98-99 0.06997026554024899 2215 100 0.11362577133456853 2216 1 -0.0013663977911377856 2216 2 -0.027511202676599567 2216 3 -0.10477010372729012 2216 4 -0.033756252543234666 2216 5 -0.019342980076238803 2216 6 -0.02886900305999518 2216 7 -0.05771112049036731 2216 8 -0.097148490560933 2216 9 -0.06111276834125334 2216 10-11 -0.1094270212293651 2216 12-13 -0.09671825738835338 2216 14-15 -0.2029998639837558 2216 16-17 -0.12818537747097736 2216 18-19 -0.09218292803368655 2216 20-21 -0.11869860067558591 2216 22-23 -0.15099143688967587 2216 24-25 -0.18460931941581293 2216 26-27 -0.22198227210261479 2216 28-29 -0.2033655675113195 2216 30-31 -0.1809820046401498 2216 32-33 -0.18236464202450975 2216 34-35 -0.15721752042251325 2216 36-37 -0.13826266785469699 2216 38-39 -0.1146261465951639 2216 40-41 -0.11925377876119114 2216 42-43 -0.1567198615299361 2216 44-45 -0.0833668073379954 2216 46-47 -0.1538037827951939 2216 48-49 -0.18064490739959638 2216 50-51 -0.16777875737391668 2216 52-53 -0.0962721682997838 2216 54-55 -0.074708938707019 2216 56-57 -0.07167011832430603 2216 58-59 -0.09436264382986792 2216 60-61 -0.023978826183942203 2216 62-63 -0.002451012403042796 2216 64-65 0.014494825388773336 2216 66-67 -0.008886649711172367 2216 68-69 0.008178669238986913 2216 70-71 0.05202854872266727 2216 72-73 0.04071063964694588 2216 74-75 0.05492211350547649 2216 76-77 0.03446587646508448 2216 78-79 -4.6107794508287725E-4 2216 80-81 0.0253648360493699 2216 82-83 0.05476802418048976 2216 84-85 0.0463096054577079 2216 86-87 0.0037596349264745754 2216 88-89 0.05101779315582888 2216 90-91 0.029295961559377304 2216 92-93 0.041717880315225386 2216 94-95 0.02035360430253519 2216 96-97 0.029243785111056297 2216 98-99 0.014679703750203998 2216 100 0.009305374185757387 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18.0 3 7.0 4 9.0 5 20.0 6 50.0 7 127.0 8 252.0 9 561.0 10 946.0 11 1674.0 12 2464.0 13 3829.0 14 4695.0 15 6317.0 16 7757.0 17 11410.0 18 12861.0 19 7089.0 20 4822.0 21 4459.0 22 4582.0 23 4904.0 24 5641.0 25 6893.0 26 8659.0 27 11126.0 28 14622.0 29 18572.0 30 24533.0 31 31556.0 32 41039.0 33 53996.0 34 75355.0 35 116309.0 36 215046.0 37 507987.0 38 1008433.0 39 563498.0 40 2683.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.48885511888173 20.13500734485675 13.65313082830647 23.72300670795505 2 15.352987351935921 20.288037736635 40.673785377197575 23.685189534231505 3 17.954065658551542 25.54889918525597 30.590875254641176 25.906159901551312 4 11.965525611966711 16.857129724589846 39.56367297808956 31.61367168535388 5 12.092322680347932 38.158992268724504 36.49904194258266 13.249643108344905 6 30.4036773941952 38.96296966602246 17.65546369970102 12.977889240081328 7 26.627787343990434 32.94196650608597 22.954768672219657 17.47547747770394 8 24.781482860665047 36.79670266335774 20.42640153146033 17.995412944516882 9 24.847582981003264 16.27818790186994 21.618223825532517 37.25600529159428 10-11 23.519637028285796 26.861361179837616 29.44239960643352 20.17660218544307 12-13 24.47173065508092 24.21471049457394 29.638616906558134 21.674941943787008 14-15 21.478932856362857 26.247141399997165 27.02315804895758 25.250767694682395 16-17 20.659788616852694 29.81697794564136 27.528591809612244 21.9946416278937 18-19 20.44007484920815 28.3962145921483 30.25099836648893 20.912712192154622 20-21 21.58375050856417 27.66116860773894 30.035126387846027 20.71995449585087 22-23 21.16531294740694 27.442431171823294 30.129162017913693 21.26309386285607 24-25 21.079668392643708 27.62049835185621 30.02521366439487 21.27461959110521 26-27 20.865884002253697 27.535181654727282 30.146303399106785 21.452630943912244 28-29 20.8194585125426 27.631083501028254 30.20833913165423 21.34111885477491 30-31 21.126131158529773 28.044289721711024 29.784758820890154 21.04482029886905 32-33 20.78129565578294 27.737829419803056 30.26304375897177 21.217831165442234 34-35 20.948851570559516 27.578506423905953 30.236485515679735 21.2361564898548 36-37 21.310915429277696 27.957031731957834 29.593188639087348 21.138864199677126 38-39 21.086418449383697 28.130274383793928 29.72415073778067 21.059156429041703 40-41 20.968982933770704 27.827300291423708 29.916836660836754 21.286880113968834 42-43 20.988583964823786 27.74198194496425 29.781883473399816 21.487550616812147 44-45 21.120778055099308 27.99694918383305 29.628618623914534 21.253654137153116 46-47 21.259746794345737 28.254561074164886 29.292333617178993 21.193358514310383 48-49 21.273020486417057 28.170928856796195 29.04402885469528 21.512021802091468 50-51 21.302752632929426 28.139436042345217 28.972557345546747 21.585253979178614 52-53 21.294111204180773 28.166925675645345 28.920664248052496 21.618298872121386 54-55 21.433852852001998 28.53068542923762 28.742755485075016 21.292706233685365 56-57 21.221118864896564 28.22322642781193 28.864131677974154 21.691523029317352 58-59 21.082026375559966 27.83610884182744 29.28435870043361 21.79750608217899 60-61 21.34437667539864 28.224674436267073 28.877597372867204 21.553351515467085 62-63 21.15281098921382 28.26247222837674 28.915543023886737 21.669173758522707 64-65 21.19338190709626 28.01393830824475 29.03295310678944 21.75972667786955 66-67 21.05927860780485 28.031655619721757 28.92294621266584 21.986119559807555 68-69 21.50814968561138 28.149344470099862 28.821249151635502 21.521256692653253 70-71 21.46702142768267 28.023863956487848 28.81889825984802 21.690216355981455 72-73 21.30883518150347 28.197382810823758 28.834344813049068 21.659437194623706 74-75 21.35504560586286 28.189847365209047 28.73464715133254 21.720459877595555 76-77 21.322373314885493 28.264121314778485 28.59222522644 21.821280143896026 78-79 21.46819213882223 28.345815981692034 28.588544451502607 21.597447427983134 80-81 21.391536010307437 28.155053925149005 28.718580170971848 21.734829893571714 82-83 21.19675580214932 28.20849494054662 28.829458051908457 21.765291205395602 84-85 21.220034325741917 28.13572511109902 28.713165317103744 21.931075246055315 86-87 21.349334995694473 28.189517788389374 28.617341174966622 21.843806040949527 88-89 21.10933599923298 28.167237084445173 28.651849809016873 22.071577107304975 90-91 21.09247381947494 28.072744208828 28.724694313665005 22.11008765803205 92-93 21.198280378583874 28.277067752466156 28.560642976387165 21.964008892562802 94-95 21.023315490047583 28.01040361591367 28.702374065507737 22.263906828531017 96-97 20.952538439910068 27.99361606089627 28.732537800726156 22.321307698467503 98-99 20.98110421534609 28.054428305649132 28.63592407500572 22.328543403999067 100 21.084522736094968 28.03464233171419 28.44856777916986 22.432267153020987 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 24.0 1 24.0 2 112.0 3 218.5 4 146.0 5 99.5 6 288.0 7 649.0 8 1114.0 9 1351.0 10 1141.5 11 908.0 12 923.5 13 1053.5 14 1218.5 15 1428.5 16 1660.5 17 1919.5 18 2221.5 19 2692.0 20 3411.0 21 4324.5 22 5820.5 23 8253.0 24 11387.5 25 15085.5 26 19911.0 27 26521.0 28 34801.0 29 43426.5 30 51977.0 31 60412.0 32 68629.0 33 77119.5 34 84201.0 35 89332.5 36 93899.0 37 99015.0 38 102599.5 39 102952.5 40 103203.0 41 103393.0 42 101680.5 43 100136.0 44 98437.0 45 95275.0 46 92940.5 47 89890.0 48 86365.0 49 83955.0 50 81052.0 51 77577.5 52 72036.5 53 67060.5 54 64177.0 55 60836.0 56 56252.0 57 50936.0 58 46365.5 59 41974.5 60 37053.5 61 31501.0 62 26522.0 63 21986.5 64 17596.5 65 14046.0 66 10827.5 67 7963.5 68 5970.5 69 4535.5 70 3165.5 71 2231.0 72 1700.0 73 1270.0 74 919.5 75 634.5 76 401.5 77 272.0 78 192.5 79 109.0 80 51.0 81 22.0 82 12.5 83 6.5 84 2.5 85 2.5 86 1.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09271757658805782 2 9.69548632020744E-4 3 0.0 4 3.23182877340248E-4 5 0.037453304562875406 6 1.0772762578008267E-4 7 0.004165468196829863 8 2.8727366874688713E-4 9 2.5136446015352625E-4 10-11 3.950012945269698E-4 12-13 0.0 14-15 8.977302148340222E-5 16-17 0.0 18-19 7.181841718672178E-5 20-21 0.0 22-23 1.4363683437344357E-4 24-25 5.3863812890041334E-5 26-27 0.0014363683437344356 28-29 0.008277072580769686 30-31 0.03734557693709532 32-33 0.025872584791516523 34-35 0.029625097089522736 36-37 0.026806224214943903 38-39 0.02542371968409951 40-41 0.031671921979344306 42-43 0.023233257959904496 44-45 0.02255098299663064 46-47 0.015225504443585017 48-49 0.010682989556524865 50-51 0.007630706826089189 52-53 0.005332517476114093 54-55 0.003878194528082976 56-57 0.0024418261843485406 58-59 9.336394234273831E-4 60-61 5.745473374937743E-4 62-63 0.0012209130921742703 64-65 0.00364478467222613 66-67 6.822749632738569E-4 68-69 0.0011131854663941876 70-71 5.565927331970938E-4 72-73 7.002295675705374E-4 74-75 5.925019417904547E-4 76-77 4.4886510741701116E-4 78-79 7.181841718672178E-5 80-81 5.386381289004134E-4 82-83 5.027289203070525E-4 84-85 3.7704669023028934E-4 86-87 2.5136446015352625E-4 88-89 0.0 90-91 7.181841718672178E-5 92-93 7.181841718672178E-5 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2784801.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.73892152494545 #Duplication Level Percentage of deduplicated Percentage of total 1 77.49998001221438 50.947651042078014 2 13.977100402177218 18.376790129700233 3 4.136157255614122 8.157195517249512 4 1.7002608712930356 4.4709326395947295 5 0.8536795252869723 2.8059985660146474 6 0.4730849311262664 1.8660055897166308 7 0.31110000095886636 1.431596498461176 8 0.20270668894304014 1.0660575293606434 9 0.14578200459847354 0.862519658404346 >10 0.6639361906481501 7.4667994873643035 >50 0.028299521998841136 1.263241168441055 >100 0.007639620426580849 0.8393491306451917 >500 5.4594942821254255E-5 0.02343625268145812 >1k 2.1837977128293467E-4 0.42242679028810376 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4636 0.16647509103882108 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3948 0.1417695555265888 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.7954604296680446E-5 0.0 20-21 0.0 0.0 0.0 3.590920859336089E-5 0.0 22-23 0.0 0.0 0.0 1.4363683437344357E-4 0.0 24-25 0.0 0.0 0.0 2.5136446015352625E-4 0.0 26-27 0.0 0.0 0.0 3.23182877340248E-4 0.0 28-29 0.0 0.0 0.0 0.0012209130921742703 0.0 30-31 1.7954604296680446E-5 0.0 0.0 0.0041654681968298635 0.0 32-33 5.386381289004134E-5 0.0 0.0 0.011203673081128598 0.0 34-35 7.181841718672178E-5 0.0 0.0 0.02136597911304973 0.0 36-37 7.181841718672178E-5 0.0 0.0 0.03987717614292727 0.0 38-39 7.181841718672178E-5 0.0 0.0 0.07022045740431722 0.0 40-41 7.181841718672178E-5 0.0 0.0 0.10878694743358681 0.0 42-43 7.181841718672178E-5 0.0 0.0 0.14852048674214063 0.0 44-45 7.181841718672178E-5 0.0 0.0 0.19010335029325254 0.0 46-47 7.181841718672178E-5 0.0 0.0 0.2428539777168997 0.0 48-49 7.181841718672178E-5 0.0 0.0 0.3049050901662273 0.0 50-51 1.0772762578008267E-4 0.0 0.0 0.3739944074998537 0.0 52-53 1.0772762578008267E-4 0.0 0.0 0.4419166755541958 0.0 54-55 1.0772762578008267E-4 0.0 0.0 0.5182416984193843 0.0 56-57 1.0772762578008267E-4 0.0 0.0 0.6031490221383862 0.0 58-59 1.2568223007676312E-4 0.0 0.0 0.7036948061997967 0.0 60-61 1.4363683437344357E-4 0.0 0.0 0.8146363061489852 0.0 62-63 1.61591438670124E-4 1.7954604296680446E-5 0.0 0.9246800758833396 0.0 64-65 1.7954604296680445E-4 3.590920859336089E-5 0.0 1.0419415965449597 0.0 66-67 1.7954604296680445E-4 3.590920859336089E-5 0.0 1.176708856395843 0.0 68-69 1.7954604296680445E-4 3.590920859336089E-5 0.0 1.3299514040680105 0.0 70-71 1.7954604296680445E-4 3.590920859336089E-5 0.0 1.4968394510056553 0.0 72-73 1.7954604296680445E-4 3.590920859336089E-5 0.0 1.6737282125365511 0.0 74-75 1.7954604296680445E-4 3.590920859336089E-5 0.0 1.8518737963682144 0.0 76-77 1.7954604296680445E-4 3.590920859336089E-5 0.0 2.0427851038548175 0.0 78-79 1.7954604296680445E-4 3.590920859336089E-5 0.0 2.26280082490634 0.0 80-81 1.7954604296680445E-4 3.590920859336089E-5 0.0 2.4993706911194016 0.0 82-83 1.7954604296680445E-4 3.590920859336089E-5 0.0 2.7506094690428506 0.0 84-85 1.7954604296680445E-4 3.590920859336089E-5 0.0 3.0072884920681946 0.0 86-87 1.7954604296680445E-4 3.590920859336089E-5 0.0 3.2706825371004964 0.0 88 1.7954604296680445E-4 3.590920859336089E-5 0.0 3.487897339881737 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3790 0.0 52.521576 1 GTATCAA 6830 0.0 41.959774 1 TCAACGC 8060 0.0 35.052853 4 ATCAACG 8100 0.0 34.821712 3 CAACGCA 8300 0.0 33.86997 5 AACGCAG 8655 0.0 32.4714 6 TATCAAC 8745 0.0 32.414654 2 ACGCAGA 9895 0.0 28.354723 7 CGCAGAG 9945 0.0 27.973873 8 GTACATG 7485 0.0 25.399557 1 GCAGAGT 11115 0.0 24.47963 9 TACATGG 7420 0.0 24.011497 2 ACATGGG 7520 0.0 22.879534 3 TGGTATC 1930 0.0 21.67787 2 GTGGTAT 2030 0.0 21.095116 1 GAGTACT 7740 0.0 20.824812 12-13 CAGAGTA 11060 0.0 20.692337 10-11 AGAGTAC 10435 0.0 20.24291 10-11 CATGGGG 4830 0.0 18.78426 4 GTACTTT 8665 0.0 18.683086 14-15 >>END_MODULE