FastQCFastQC Report
Wed 25 May 2016
SRR1033175_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033175_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943286
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC24600.033.8031044
GGTATCA16050.033.4563981
ATCAACG24750.033.412723
GTATCAA26750.032.7520521
TATACCG1309.235009E-932.5241855
CAACGCA26450.031.2603785
AACGCAG27250.030.331716
GTACATG55300.028.0233541
TATCAAC30450.027.7918112
TACATGG54500.026.6559182
ACGCAGA33150.024.5083077
ACATGGG57650.023.8804573
CGCAGAG33750.023.6551598
TACACCG1852.8287832E-722.8548345
TACCGCG1656.904238E-519.9234628
ACCGTTC1708.684466E-519.3374778
GCAGAGT40200.019.0419869
GAGTACA42000.018.9533841
GTATAGA8750.018.8412341
GTCTAAC3106.200935E-918.2334521
Produced by FastQC (version 0.11.5)