##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033175_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1943286
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.560001461442113	33.0	31.0	34.0	30.0	34.0
2	31.714617405775577	34.0	31.0	34.0	30.0	34.0
3	31.792845726259543	34.0	31.0	34.0	30.0	34.0
4	35.13863836820725	37.0	35.0	37.0	33.0	37.0
5	35.24630033870465	37.0	35.0	37.0	33.0	37.0
6	35.27737399435801	37.0	36.0	37.0	33.0	37.0
7	35.242921525704396	37.0	36.0	37.0	33.0	37.0
8	35.25169120757315	37.0	36.0	37.0	33.0	37.0
9	36.89061311613422	39.0	38.0	39.0	34.0	39.0
10-11	36.892546953973834	39.0	38.0	39.0	34.0	39.0
12-13	36.853406292228726	39.0	38.0	39.0	34.0	39.0
14-15	38.30812345686636	41.0	39.0	41.0	34.0	41.0
16-17	38.31121692843977	41.0	39.0	41.0	34.0	41.0
18-19	38.300774307024284	41.0	39.0	41.0	34.0	41.0
20-21	38.34038530612581	41.0	39.0	41.0	34.0	41.0
22-23	38.303220421492256	41.0	39.0	41.0	34.0	41.0
24-25	38.300179181036654	41.0	39.0	41.0	34.0	41.0
26-27	38.264119383353766	40.5	39.0	41.0	34.0	41.0
28-29	38.23721727012905	40.0	39.0	41.0	33.5	41.0
30-31	38.2311960257008	40.0	39.0	41.0	33.0	41.0
32-33	38.297283570200165	41.0	39.0	41.0	34.0	41.0
34-35	38.26638976455344	41.0	39.0	41.0	33.5	41.0
36-37	38.1878099775329	41.0	39.0	41.0	33.0	41.0
38-39	38.113330719204484	41.0	38.0	41.0	33.0	41.0
40-41	38.05146746284386	41.0	38.0	41.0	33.0	41.0
42-43	37.934788548880604	40.5	38.0	41.0	33.0	41.0
44-45	37.806022376531295	40.0	38.0	41.0	33.0	41.0
46-47	37.6841754121627	40.0	38.0	41.0	32.5	41.0
48-49	37.56818811024213	40.0	38.0	41.0	32.0	41.0
50-51	37.44657039674037	40.0	37.5	41.0	32.0	41.0
52-53	37.301420377648995	40.0	37.0	41.0	31.5	41.0
54-55	37.15324790072074	40.0	37.0	41.0	31.0	41.0
56-57	36.99996166287412	40.0	36.5	41.0	31.0	41.0
58-59	36.80789960921861	40.0	36.0	41.0	31.0	41.0
60-61	36.5873203944247	39.5	35.5	41.0	31.0	41.0
62-63	36.32893202544556	39.0	35.0	41.0	30.0	41.0
64-65	36.06763440893415	39.0	35.0	41.0	30.0	41.0
66-67	35.77886888497113	38.0	35.0	40.5	30.0	41.0
68-69	35.4767517493565	37.5	35.0	40.0	30.0	41.0
70-71	35.13505320369724	37.0	35.0	40.0	29.0	41.0
72-73	34.77424450132405	36.5	34.5	39.0	29.0	41.0
74-75	34.40153842512116	36.0	34.0	39.0	29.0	41.0
76-77	34.07093757686722	35.5	34.0	38.5	29.0	40.0
78-79	33.69922595027186	35.0	34.0	37.0	28.0	39.5
80-81	33.338721371944224	35.0	34.0	37.0	28.0	39.0
82-83	33.112496565096436	35.0	34.0	36.5	28.0	39.0
84-85	32.8378769259903	35.0	34.0	36.0	28.0	37.5
86-87	32.58113808260853	35.0	34.0	36.0	27.0	37.0
88-89	32.213316516457176	35.0	34.0	35.5	26.5	37.0
90-91	32.02157814135439	35.0	34.0	35.0	26.0	36.0
92-93	31.83881811529543	35.0	33.0	35.0	26.0	36.0
94-95	31.666272231673567	35.0	33.0	35.0	25.5	36.0
96-97	31.49036837603935	35.0	33.0	35.0	25.0	36.0
98-99	31.29891045373661	35.0	33.0	35.0	25.0	35.5
100	31.138232354887545	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22221720963324998
1101	2	0.22304504935797098
1101	3	0.24699618609170315
1101	4	0.21332678622640344
1101	5	0.25492018576407105
1101	6	0.23563366009727105
1101	7	0.1368509784300258
1101	8	0.24864322219733737
1101	9	0.3154665537629242
1101	10-11	0.25966630394037793
1101	12-13	0.24965743801030982
1101	14-15	0.3010672224179558
1101	16-17	0.15136468640861978
1101	18-19	0.15458373696509398
1101	20-21	0.16984143866489632
1101	22-23	0.16970709686997765
1101	24-25	0.15343356677761477
1101	26-27	0.16944453076868626
1101	28-29	0.2838746588881236
1101	30-31	0.345629948985021
1101	32-33	0.21400617038854364
1101	34-35	0.26013160341947383
1101	36-37	0.2749640962352373
1101	38-39	0.2040987316149696
1101	40-41	0.23289985914723133
1101	42-43	0.1991487618007639
1101	44-45	0.1922086343501732
1101	46-47	0.23872293375130482
1101	48-49	0.16040584018038118
1101	50-51	0.13646045913021965
1101	52-53	0.1871969568694567
1101	54-55	0.23827150409993436
1101	56-57	0.2874385066364411
1101	58-59	0.20889624025066666
1101	60-61	0.18945387594542495
1101	62-63	0.2036531687667562
1101	64-65	0.27611516232655475
1101	66-67	0.17051769494203484
1101	68-69	0.09469624834675727
1101	70-71	0.1664632428716999
1101	72-73	0.21262464892533472
1101	74-75	0.1276412414346666
1101	76-77	0.04506504973657144
1101	78-79	0.0590670314134627
1101	80-81	0.05811648944662551
1101	82-83	0.05206959210914164
1101	84-85	0.008517962094131803
1101	86-87	0.1430188361677267
1101	88-89	0.17765602068270425
1101	90-91	0.26231051481495626
1101	92-93	0.25495990348915853
1101	94-95	0.21634816460898065
1101	96-97	0.24584354430809086
1101	98-99	0.32328198676329833
1101	100	0.3896182781479851
1102	1	0.03262972302455225
1102	2	0.08252511828936093
1102	3	0.08464717244529041
1102	4	-0.025840237519105358
1102	5	-0.08635479677312219
1102	6	-0.07685692401357613
1102	7	-0.019019633731915064
1102	8	0.043164209868642445
1102	9	0.03953005296831691
1102	10-11	0.03655036182342997
1102	12-13	0.08175600013238693
1102	14-15	0.07178949546518254
1102	16-17	0.1050418836303777
1102	18-19	0.06649093719858712
1102	20-21	0.013168191497150872
1102	22-23	-0.046508085920002884
1102	24-25	0.027127801279483776
1102	26-27	0.10389382483157306
1102	28-29	0.04186948215533448
1102	30-31	0.0763765209270062
1102	32-33	0.06383459046539741
1102	34-35	0.005894638752813819
1102	36-37	0.0661804692880068
1102	38-39	0.018634005137364795
1102	40-41	0.07348334283131663
1102	42-43	0.05170109661083444
1102	44-45	-0.012090365599796371
1102	46-47	0.009924076835190476
1102	48-49	0.009173476469513275
1102	50-51	-0.008947844170052122
1102	52-53	-0.10683228030089253
1102	54-55	-0.03473330860799706
1102	56-57	-0.17929226562898748
1102	58-59	-0.07911997807467941
1102	60-61	-0.10980159275577961
1102	62-63	-0.06816535992275163
1102	64-65	-0.056206548781759125
1102	66-67	-0.08668980151516337
1102	68-69	-0.01285556112919295
1102	70-71	-0.06623030228929139
1102	72-73	-0.008417336128204056
1102	74-75	-0.02617712837331254
1102	76-77	-0.03199357552810511
1102	78-79	0.024601269238623047
1102	80-81	0.14727782825422508
1102	82-83	0.09935903664283074
1102	84-85	0.039749378989711204
1102	86-87	0.04485216457815966
1102	88-89	0.1702078079044469
1102	90-91	0.18666132255494006
1102	92-93	0.19283297332460592
1102	94-95	0.1533308639200044
1102	96-97	0.15063964925568385
1102	98-99	0.30340716059898654
1102	100	0.34218215817003284
1103	1	-0.00953055333992836
1103	2	-0.007755943210565164
1103	3	0.0912197129961747
1103	4	0.02706601319550117
1103	5	0.01754341328690856
1103	6	-0.019435542564409047
1103	7	0.0670576355102881
1103	8	0.0746647246482155
1103	9	0.050583160294536356
1103	10-11	0.16487990275886233
1103	12-13	0.16188879407520318
1103	14-15	0.13142027615594998
1103	16-17	0.13938735395123558
1103	18-19	0.11309728209013059
1103	20-21	0.0738285970493564
1103	22-23	0.0402004684102053
1103	24-25	0.06704273451321541
1103	26-27	0.01840184301300951
1103	28-29	0.06785776825871181
1103	30-31	0.11952673273967918
1103	32-33	0.06475539799513541
1103	34-35	0.048767944625979
1103	36-37	0.14191851495501595
1103	38-39	0.03719793965667151
1103	40-41	0.13704238977022953
1103	42-43	0.018882223059463854
1103	44-45	-0.002707039201162331
1103	46-47	0.024508227722250808
1103	48-49	0.06431304521118619
1103	50-51	-0.022639246102635013
1103	52-53	-0.04299028201515398
1103	54-55	0.053691418579859373
1103	56-57	-0.10111338675766035
1103	58-59	-0.10322544835348424
1103	60-61	-0.07430243530157554
1103	62-63	-0.0790926307814459
1103	64-65	-0.009015967841769168
1103	66-67	0.00960446893691369
1103	68-69	-0.050667284015084135
1103	70-71	-0.06359503038658687
1103	72-73	-0.13292861094427622
1103	74-75	-0.04986947391512331
1103	76-77	-0.10977812299388034
1103	78-79	-0.08804733935831166
1103	80-81	-0.07806827788577664
1103	82-83	-0.11106792419160882
1103	84-85	-0.10609620395971575
1103	86-87	-0.1467996010356174
1103	88-89	-0.06469313021134582
1103	90-91	-0.05777696842418578
1103	92-93	0.06644236536427428
1103	94-95	0.03208529185230802
1103	96-97	0.05802591477365482
1103	98-99	0.1086444415219674
1103	100	0.09708649485611431
1104	1	0.14398902648976986
1104	2	0.08164998312687288
1104	3	0.11171710786679512
1104	4	0.16924699973379376
1104	5	0.11261148773785123
1104	6	0.20305077820861328
1104	7	0.15418522251303557
1104	8	0.12704176478824536
1104	9	0.1604014650303327
1104	10-11	0.21586451094781012
1104	12-13	0.16846506074404743
1104	14-15	0.10653406547032063
1104	16-17	0.13518925076319732
1104	18-19	0.1464811827786363
1104	20-21	0.06386303255018788
1104	22-23	0.17015886905888777
1104	24-25	0.17207943711997586
1104	26-27	0.2573077039924385
1104	28-29	0.2505863783185305
1104	30-31	0.18352371197421746
1104	32-33	0.21407789585568082
1104	34-35	0.19474109984512467
1104	36-37	0.20536058937736357
1104	38-39	0.1853239519398855
1104	40-41	0.18876636043265194
1104	42-43	0.24890245851169368
1104	44-45	0.1250392736880528
1104	46-47	0.17136256718766418
1104	48-49	0.07799635706487607
1104	50-51	0.09957614529992753
1104	52-53	0.07917034652464139
1104	54-55	0.052200183701550884
1104	56-57	0.026630756041413406
1104	58-59	0.011795966606555908
1104	60-61	0.09194778681353455
1104	62-63	0.05445535425156578
1104	64-65	0.025043341487467785
1104	66-67	0.15988614663614698
1104	68-69	0.12669635262707146
1104	70-71	0.1481719386970326
1104	72-73	0.2161790224626543
1104	74-75	0.1900281182549861
1104	76-77	0.12040155025692201
1104	78-79	0.1497558605289413
1104	80-81	0.20358759279523042
1104	82-83	0.26965996488767985
1104	84-85	0.22581435871146738
1104	86-87	0.2161260174655979
1104	88-89	0.37996140165171255
1104	90-91	0.36790369949381585
1104	92-93	0.18614249465058919
1104	94-95	0.23548209564143718
1104	96-97	0.26100414647187975
1104	98-99	0.3496009100245132
1104	100	0.3443350758724719
1105	1	-0.03877898987571271
1105	2	0.020038289192498837
1105	3	0.048946880606216325
1105	4	-0.021213959746084754
1105	5	0.012751551586553944
1105	6	-0.06733610413775182
1105	7	-0.036538857778900535
1105	8	-0.009104061965047094
1105	9	-0.12780523179520742
1105	10-11	-0.05478901506543821
1105	12-13	0.004448411003281194
1105	14-15	0.03462925786678284
1105	16-17	0.05644658194555774
1105	18-19	-0.05460470685154206
1105	20-21	0.003460649642597957
1105	22-23	0.006837557973796038
1105	24-25	-0.008305914606708598
1105	26-27	0.060346895144974155
1105	28-29	0.1299648616930824
1105	30-31	0.06706068251342856
1105	32-33	0.08912472552929529
1105	34-35	0.07336605672052343
1105	36-37	0.06133614076488669
1105	38-39	0.05191429008535664
1105	40-41	0.08438137799833356
1105	42-43	0.0011969910410059015
1105	44-45	0.039694592712926635
1105	46-47	0.06384989705806987
1105	48-49	0.06488234776454505
1105	50-51	0.05998314436211416
1105	52-53	0.08990102305854464
1105	54-55	0.08915772479747375
1105	56-57	0.055625369717866135
1105	58-59	0.024350449378573558
1105	60-61	-0.031711159537032074
1105	62-63	0.0518622294064528
1105	64-65	0.06873615583545245
1105	66-67	0.08538669571559154
1105	68-69	-0.027474403790598956
1105	70-71	-0.052996616543758535
1105	72-73	-0.1471305052804297
1105	74-75	-0.07225449730680111
1105	76-77	-0.005353676930766937
1105	78-79	-0.03287458228175666
1105	80-81	-0.027623260999000365
1105	82-83	-0.03419482789556838
1105	84-85	-0.07037081468588013
1105	86-87	-0.021522246131404188
1105	88-89	0.06481506955096705
1105	90-91	0.11892101493266694
1105	92-93	0.1300044667690159
1105	94-95	0.0847738196051111
1105	96-97	0.2013273501803532
1105	98-99	0.13354331256291374
1105	100	0.1573981859944844
1106	1	0.03789924450027726
1106	2	0.07328231793782791
1106	3	-0.011633127303195323
1106	4	-0.03499388622131505
1106	5	-0.057459217849419986
1106	6	0.03060276874770551
1106	7	0.03451761027105249
1106	8	-0.0037785475607918784
1106	9	0.03718649608359925
1106	10-11	-0.09900192331860325
1106	12-13	-0.09564682954345471
1106	14-15	-0.028218387679260104
1106	16-17	-0.07868331492787917
1106	18-19	-0.08150279010629191
1106	20-21	-0.12938119254826574
1106	22-23	-0.09328248723621613
1106	24-25	-0.04012959557194051
1106	26-27	0.010747572359079527
1106	28-29	0.029467890904086858
1106	30-31	-0.020419105196097576
1106	32-33	-0.06042533893942448
1106	34-35	-0.04741176837852379
1106	36-37	-0.00910231675947415
1106	38-39	-0.0809422944997138
1106	40-41	-0.11308842934430885
1106	42-43	-0.028190158271364396
1106	44-45	-0.0807262558405597
1106	46-47	-0.0683142045100027
1106	48-49	-0.060745725030123765
1106	50-51	-0.0018648264526248681
1106	52-53	0.026000817367041407
1106	54-55	0.03308175232596966
1106	56-57	-0.009705374726237892
1106	58-59	-0.008493966881118808
1106	60-61	-0.008030135806457395
1106	62-63	0.0699049308682902
1106	64-65	-0.009350269695566737
1106	66-67	0.1256582365765908
1106	68-69	0.0017149517327652575
1106	70-71	0.026744174724996128
1106	72-73	0.061082529336118796
1106	74-75	0.10938964235749182
1106	76-77	0.048048130533565825
1106	78-79	-0.009214560612775813
1106	80-81	-0.007266120649070729
1106	82-83	-0.01660176945718206
1106	84-85	0.006794913225689925
1106	86-87	0.02430208771128406
1106	88-89	0.07612061386122093
1106	90-91	0.10546524562539616
1106	92-93	0.17859270701855934
1106	94-95	0.10779488718846153
1106	96-97	0.08210683672382046
1106	98-99	0.1448303118772749
1106	100	0.2344694338796529
1107	1	0.26013680678648754
1107	2	0.27920102932401036
1107	3	0.2558233609080638
1107	4	0.348766375221814
1107	5	0.2569483115384088
1107	6	0.2621843979714953
1107	7	0.24935530734798306
1107	8	0.2702035735946353
1107	9	0.4140264711600281
1107	10-11	0.3241709993692936
1107	12-13	0.28659862581120876
1107	14-15	0.35592699010716444
1107	16-17	0.35219193462569365
1107	18-19	0.3359235881833271
1107	20-21	0.3553676552817393
1107	22-23	0.34419900628856936
1107	24-25	0.3334770825634479
1107	26-27	0.31017371267586213
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2216	3	-0.07255801294640207
2216	4	-0.006375396533492506
2216	5	-0.08212938878273235
2216	6	0.014827148813509439
2216	7	-0.05579977998655039
2216	8	0.026449363521791724
2216	9	-0.08776134116862977
2216	10-11	-0.07889177967041405
2216	12-13	-0.05638954682135733
2216	14-15	-0.07929996219173319
2216	16-17	-0.05404504352109285
2216	18-19	-0.11182118349365311
2216	20-21	-0.10858530939281508
2216	22-23	-0.1729843570700922
2216	24-25	-0.09269018460973655
2216	26-27	-0.21025395239816191
2216	28-29	-0.11133621791542936
2216	30-31	-0.15342388009376862
2216	32-33	-0.08577462872355568
2216	34-35	-0.11019766036151424
2216	36-37	-0.2373731216707924
2216	38-39	-0.2569390042340842
2216	40-41	-0.14776421780430837
2216	42-43	-0.24692781977525868
2216	44-45	-0.08038940486552093
2216	46-47	-0.01998220868302525
2216	48-49	-0.044912324821467564
2216	50-51	-0.05638452792120319
2216	52-53	-0.1176226879696074
2216	54-55	-0.16876882146425487
2216	56-57	-0.09200655440684358
2216	58-59	-0.0831394608222098
2216	60-61	-0.029636949063821305
2216	62-63	-0.036008318469647804
2216	64-65	-0.016816765161415503
2216	66-67	-0.06380990402207942
2216	68-69	-0.026698498231120027
2216	70-71	-0.013731910661405777
2216	72-73	0.07319993564379246
2216	74-75	0.008517120088008312
2216	76-77	0.039446292799830474
2216	78-79	-0.02295202149072395
2216	80-81	-0.0069094077046898406
2216	82-83	-0.08138027840510631
2216	84-85	-0.024199388997246274
2216	86-87	-0.015231256322103093
2216	88-89	0.03233600588433205
2216	90-91	0.06069656734917572
2216	92-93	0.101370373337474
2216	94-95	0.07993676986239961
2216	96-97	0.019589382265913002
2216	98-99	0.07860107666341065
2216	100	0.16668635066000803
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	23254.0
3	3921.0
4	2533.0
5	2023.0
6	1774.0
7	1912.0
8	2071.0
9	2283.0
10	2540.0
11	2915.0
12	3282.0
13	3525.0
14	3962.0
15	3942.0
16	4007.0
17	3961.0
18	3985.0
19	3949.0
20	4106.0
21	4350.0
22	4911.0
23	6778.0
24	10283.0
25	14394.0
26	15285.0
27	13059.0
28	13335.0
29	16806.0
30	20552.0
31	24940.0
32	32077.0
33	44432.0
34	62284.0
35	94317.0
36	177433.0
37	381400.0
38	637104.0
39	288870.0
40	731.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.75245472752873	20.014942380960495	13.605724573814278	23.626878317696498
2	15.124784066712243	20.092602085633487	41.180725535846854	23.60188831180741
3	17.996809510911824	25.69888497658626	29.937662910100915	26.366642602401
4	12.031190717533736	16.96035162916261	39.45022422124879	31.558233432054863
5	12.675513244641035	38.17084232972242	35.901332649659246	13.252311775977299
6	30.453869166323997	38.617811911215775	18.01432046741973	12.913998455040495
7	27.12455703550612	32.700531814705755	22.68889097026593	17.4860201795222
8	24.732830320747727	37.16051797257792	20.401809610079464	17.704842096594888
9	24.68328565590519	17.439439029336288	21.385050732969688	36.49222458178883
10-11	23.616265316353537	27.24602106793188	29.056884239609893	20.080829376104685
12-13	24.179282158019113	24.63099253531424	29.040424900608535	22.14930040605811
14-15	21.591705991544067	26.643004400903497	26.039472509214978	25.725817098337455
16-17	21.34565523133797	29.996950407448725	26.620343741837193	22.03705061937611
18-19	21.198592513081636	28.97361180773747	28.544021024290807	21.283774654890088
20-21	22.450043185582707	28.27041998622591	27.99547863935046	21.28405818884093
22-23	22.077488945995313	28.009491698495236	28.080285708256625	21.832733647252827
24-25	21.853182623385642	28.170121844663814	28.000183244409175	21.97651228754136
26-27	21.625387952997855	28.123414089320548	28.115436283062863	22.13576167461874
28-29	21.71566363038614	28.156138334982504	28.196439072156593	21.931758962474763
30-31	21.918257348119646	28.56149485454053	27.794977348037293	21.72527044930253
32-33	21.533011747913612	28.263091291534437	28.324472705345276	21.879424255206676
34-35	21.713448268319453	27.998270699813304	28.32392983467427	21.964351197192975
36-37	22.028798967418144	28.386454465193463	27.73916088828596	21.845585679102438
38-39	21.8190721856411	28.55704373299681	27.953240783152804	21.67064329820928
40-41	21.593655699904048	28.189049602992334	28.282174063744066	21.935120633359553
42-43	21.607469834626954	28.100603050143018	28.232504186561624	22.0594229286684
44-45	21.752531187768884	28.25293685198904	28.17234437894519	21.822187581296884
46-47	21.819612977399967	28.45575651279787	27.963789098594965	21.760841411207203
48-49	21.680230608888877	28.285406031843806	28.003629058922545	22.030734300344765
50-51	21.695067199957183	28.1905827427134	28.09905638044501	22.015293676884408
52-53	21.759158596154098	28.201690722742416	28.102109299232396	21.937041381871087
54-55	21.823576801282613	28.530487677794508	27.9075266587228	21.738408862200075
56-57	21.688749304637483	28.282998778837925	27.97670477673524	22.051547139789353
58-59	21.54225537660969	27.903384670487107	28.318620730823703	22.235739222079502
60-61	21.899673145351382	28.25400231626037	27.899873328706988	21.946451209681257
62-63	21.66621638659246	28.318010945665346	27.944046911464643	22.071725756277548
64-65	21.69692282120437	28.075287625158175	28.08987665451686	22.137912899120593
66-67	21.52003087610961	28.127801363694843	27.997787212144605	22.354380548050944
68-69	21.949570880328938	28.32519178838244	27.85270343462985	21.872533896658773
70-71	21.8698837018719	28.069328597596225	28.00924847876367	22.0515392217682
72-73	21.58826152366872	28.20248856233409	28.137597288666594	22.0716526253306
74-75	21.698569554676954	28.188680662871974	28.021923168562214	22.090826613888858
76-77	21.71760952837488	28.221781884735357	27.908974162035786	22.151634424853974
78-79	21.812705410605073	28.32334514282156	27.923388678998656	21.940560767574706
80-81	21.72292575385496	28.10174597522041	28.05105670405494	22.124271566869698
82-83	21.539852951582088	28.15803523758676	28.256118976707683	22.045992834123467
84-85	21.562411792963918	28.067855188265494	28.11020743078586	22.259525587984726
86-87	21.76044177776337	28.14712236963071	28.020786938511698	22.071648914094222
88-89	21.55425276876708	28.0721387335908	28.08417600720269	22.289432490439427
90-91	21.479176164878865	28.00017294189437	28.17637691391403	22.34427397931273
92-93	21.670207351403857	28.18831196064533	27.967172338234374	22.174308349716444
94-95	21.4561036262295	27.912785778054804	28.18222080833615	22.448889787379546
96-97	21.427645149341913	27.90564642439023	28.21273479745601	22.453973628811845
98-99	21.4672321348841	27.968960505660075	28.109637333326432	22.454170026129393
100	21.670351651616983	27.888821993743377	28.029629660306682	22.411196694332958
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	318.0
1	292.0
2	267.5
3	237.5
4	193.0
5	161.0
6	136.0
7	126.5
8	143.5
9	172.5
10	163.5
11	157.5
12	173.0
13	188.5
14	230.5
15	269.0
16	333.0
17	481.0
18	695.5
19	942.0
20	1333.5
21	2046.0
22	3086.0
23	4634.0
24	6763.5
25	9180.5
26	12501.5
27	16858.5
28	21919.0
29	27627.0
30	33364.5
31	39066.0
32	44592.5
33	49736.5
34	54432.5
35	58780.0
36	62831.0
37	66012.0
38	68284.0
39	69818.0
40	71168.0
41	71974.0
42	70801.0
43	69758.0
44	69054.5
45	67304.0
46	66089.0
47	64296.0
48	62197.5
49	60741.5
50	58927.5
51	56842.5
52	53344.0
53	50148.5
54	48243.5
55	45691.5
56	42155.0
57	38327.5
58	34867.0
59	31629.5
60	28441.0
61	24581.5
62	20683.5
63	17481.5
64	14263.5
65	11196.5
66	8787.0
67	6716.5
68	5040.0
69	3836.0
70	2829.5
71	2062.5
72	1521.0
73	1125.5
74	841.5
75	593.0
76	408.0
77	283.0
78	199.0
79	139.5
80	87.0
81	60.0
82	36.5
83	25.5
84	23.5
85	18.0
86	9.5
87	8.5
88	10.5
89	6.5
90	3.0
91	3.0
92	3.5
93	2.0
94	2.0
95	1.0
96	0.5
97	2.0
98	2.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33515396086834365
2	0.11799601293890863
3	0.06504446592009616
4	0.04039549505322428
5	0.04322575266841834
6	0.009931631267862784
7	0.006586781358997079
8	0.016775708773695688
9	0.018936996407116605
10-11	0.022024550169146485
12-13	0.017367489911418083
14-15	0.020043369838510648
16-17	0.02076379904965095
18-19	0.020094829067877813
20-21	0.02614128851851966
22-23	0.016724249544328523
24-25	0.02704182503244504
26-27	0.02037785482939722
28-29	0.020249206755979306
30-31	0.020995365581803194
32-33	0.026527232738773398
34-35	0.015617876112934484
36-37	0.010060279341280697
38-39	0.013791073470400136
40-41	0.00964860550634338
42-43	0.00843931361621501
44-45	0.009082553983304568
46-47	0.008722339377734414
48-49	0.004245386422791087
50-51	0.00668969981773141
52-53	0.007847532478492614
54-55	0.003422038752916452
56-57	0.002830257615194058
58-59	0.0027787983858268933
60-61	0.0034734979822836163
62-63	0.0026244206977253993
64-65	0.002315665321522411
66-67	0.0018525322572179289
68-69	0.0032676610648149575
70-71	0.0024443133949403227
72-73	0.0018268026425343467
74-75	0.002315665321522411
76-77	0.004091008734689593
78-79	0.003833712587853769
80-81	0.003807982973170187
82-83	0.0024957726243074875
84-85	0.0030618241473462987
86-87	0.0023928541655731583
88-89	0.6064727477067194
90-91	0.617587941250027
92-93	0.6137542286621732
94-95	0.5995772109715194
96-97	0.596644034897591
98-99	0.5945342064935373
100	0.595537661466197
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1943286.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.99966645069887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.67805389921371	60.35470357435503
2	11.431924495037462	16.690533500546174
3	2.9157460406488247	6.385454652669302
4	1.126472395481695	3.2892843654433386
5	0.5677556669132878	2.072298715508204
6	0.3228002885900086	1.4138588038355977
7	0.2049994169972911	1.0475422344372982
8	0.13840932481648216	0.8083067636215708
9	0.10620592581917032	0.697769744389839
>10	0.48654094092397654	6.054286670863365
>50	0.017841391204875604	0.8587953385826803
>100	0.0032502143531757295	0.32716563574762425
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	2.5729614683582345E-5	0.0
12-13	0.0	0.0	0.0	7.718884405074703E-5	0.0
14-15	0.0	0.0	0.0	1.286480734179117E-4	0.0
16-17	0.0	0.0	0.0	1.5437768810149405E-4	0.0
18-19	0.0	0.0	0.0	2.0583691746865876E-4	0.0
20-21	0.0	0.0	0.0	2.0583691746865876E-4	0.0
22-23	0.0	0.0	0.0	2.830257615194058E-4	0.0
24-25	0.0	0.0	0.0	4.116738349373175E-4	0.0
26-27	0.0	0.0	0.0	4.888626789880646E-4	0.0
28-29	0.0	0.0	0.0	0.0013636695782298642	0.0
30-31	0.0	0.0	0.0	0.00416819757874034	0.0
32-33	0.0	0.0	0.0	0.010497682790901597	0.0
34-35	0.0	0.0	0.0	0.019528777544838997	0.0
36-37	0.0	0.0	0.0	0.03679334899752275	0.0
38-39	0.0	0.0	0.0	0.06460706247047526	0.0
40-41	0.0	0.0	0.0	0.09923912383457711	0.0
42-43	0.0	0.0	0.0	0.13631549859361924	0.0
44-45	0.0	0.0	0.0	0.1786664443627958	0.0
46-47	0.0	0.0	0.0	0.23043442910616346	0.0
48-49	0.0	0.0	0.0	0.28907222097004764	0.0
50-51	0.0	0.0	0.0	0.35504295301875277	0.0
52-53	0.0	0.0	0.0	0.4191354231955564	0.0
54-55	0.0	0.0	0.0	0.48839954592376006	0.0
56-57	0.0	0.0	0.0	0.5727669524712267	0.0
58-59	0.0	0.0	0.0	0.6665256683782006	0.0
60-61	0.0	0.0	0.0	0.7684921313692374	0.0
62-63	0.0	0.0	0.0	0.8731087446726833	0.0
64-65	0.0	0.0	0.0	0.9848267316287977	0.0
66-67	0.0	0.0	0.0	1.111287787798605	0.0
68-69	0.0	0.0	0.0	1.2537526643016004	0.0
70-71	0.0	0.0	0.0	1.4069467901276498	0.0
72-73	0.0	0.0	0.0	1.5780229981587888	0.0
74-75	0.0	0.0	0.0	1.7443906867028323	0.0
76-77	0.0	0.0	0.0	1.9230056718362607	0.0
78-79	0.0	0.0	0.0	2.128765400460869	0.0
80-81	0.0	0.0	0.0	2.35415682508905	0.0
82-83	0.0	0.0	0.0	2.594754451995229	0.0
84-85	0.0	0.0	0.0	2.8291255121479804	0.0
86-87	0.0	0.0	0.0	3.0814301137351885	0.0
88	0.0	0.0	0.0	3.2904060441952447	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	2460	0.0	33.803104	4
GGTATCA	1605	0.0	33.456398	1
ATCAACG	2475	0.0	33.41272	3
GTATCAA	2675	0.0	32.752052	1
TATACCG	130	9.235009E-9	32.524185	5
CAACGCA	2645	0.0	31.260378	5
AACGCAG	2725	0.0	30.33171	6
GTACATG	5530	0.0	28.023354	1
TATCAAC	3045	0.0	27.791811	2
TACATGG	5450	0.0	26.655918	2
ACGCAGA	3315	0.0	24.508307	7
ACATGGG	5765	0.0	23.880457	3
CGCAGAG	3375	0.0	23.655159	8
TACACCG	185	2.8287832E-7	22.854834	5
TACCGCG	165	6.904238E-5	19.923462	8
ACCGTTC	170	8.684466E-5	19.337477	8
GCAGAGT	4020	0.0	19.041986	9
GAGTACA	4200	0.0	18.953384	1
GTATAGA	875	0.0	18.841234	1
GTCTAAC	310	6.200935E-9	18.233452	1
>>END_MODULE