FastQCFastQC Report
Wed 25 May 2016
SRR1033175_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033175_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943286
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39490.20321249677093334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33180.17074172304025245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29250.053.5654561
GTATCAA55500.042.896711
ATCAACG63700.037.1177143
TCAACGC63850.037.0305144
CAACGCA64750.036.515815
AACGCAG67850.034.976996
TATCAAC69000.034.4028932
ACGCAGA77100.030.5368527
CGCAGAG79300.029.686628
GCAGAGT87600.026.5520049
GTACATG58200.026.516571
TACATGG57100.025.190572
ACATGGG58850.023.4829983
GAGTACT59200.022.50233312-13
GTGGTAT12300.022.186531
TATAACG1703.4500863E-622.1204382
AGAGTAC80750.021.90880210-11
CTGTCGC5600.021.8163249
CAGAGTA86250.021.7102810-11
CATGGGG37250.020.4428484
Produced by FastQC (version 0.11.5)