FastQCFastQC Report
Wed 25 May 2016
SRR1033174_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033174_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2820264
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23200.036.7669681
GTATCAA35200.033.6045651
TCAACGC36400.031.3532714
ATCAACG37200.030.5592633
CAACGCA38800.029.5354635
AACGCAG40000.028.5254086
GTACATG86950.028.2380921
TACATGG86600.026.5965962
TATCAAC45250.025.5543252
ACATGGG89350.024.867783
ACGCAGA49350.022.2645687
CATGGGG60750.021.4919854
CGCAGAG51400.021.2856088
ACCGTCC2151.2059772E-619.65618
GAGTACA60850.019.5167351
TATACCG1456.63518E-419.4343035
AGTACAT61800.017.1896822
GCAGAGT62850.016.361849
ATGGGGG32450.015.9207555
ACACCGT5900.015.9170866
Produced by FastQC (version 0.11.5)