##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033174_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2820264
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.609993248858974	33.0	31.0	34.0	30.0	34.0
2	31.767845137901983	34.0	31.0	34.0	30.0	34.0
3	31.89426911806838	34.0	31.0	34.0	30.0	34.0
4	35.23351537302891	37.0	35.0	37.0	33.0	37.0
5	35.31560662406073	37.0	35.0	37.0	33.0	37.0
6	35.419933736699825	37.0	36.0	37.0	35.0	37.0
7	35.36904807493199	37.0	37.0	37.0	33.0	37.0
8	35.36984906377559	37.0	37.0	37.0	33.0	37.0
9	37.033205402047464	39.0	38.0	39.0	35.0	39.0
10-11	37.02515846034272	39.0	38.0	39.0	34.5	39.0
12-13	36.972896508979304	39.0	38.0	39.0	34.0	39.0
14-15	38.43163512352035	41.0	39.0	41.0	34.0	41.0
16-17	38.43649761157111	41.0	39.0	41.0	34.0	41.0
18-19	38.42611223630128	41.0	39.0	41.0	34.0	41.0
20-21	38.44938860333642	41.0	39.0	41.0	34.0	41.0
22-23	38.412576978609096	41.0	39.0	41.0	34.0	41.0
24-25	38.40345265549608	41.0	39.0	41.0	34.0	41.0
26-27	38.35865968575992	40.5	39.0	41.0	34.0	41.0
28-29	38.324071966312374	40.0	39.0	41.0	34.0	41.0
30-31	38.322096796611945	40.0	39.0	41.0	34.0	41.0
32-33	38.38793992335469	41.0	39.0	41.0	34.0	41.0
34-35	38.356992288665175	41.0	39.0	41.0	34.0	41.0
36-37	38.27910614041806	41.0	39.0	41.0	33.5	41.0
38-39	38.20070071454303	41.0	39.0	41.0	33.0	41.0
40-41	38.13945981652781	41.0	38.0	41.0	33.0	41.0
42-43	38.02589810741122	40.5	38.0	41.0	33.0	41.0
44-45	37.90060806364227	40.0	38.0	41.0	33.0	41.0
46-47	37.77342794858921	40.0	38.0	41.0	33.0	41.0
48-49	37.65101919536611	40.0	38.0	41.0	32.5	41.0
50-51	37.52733219301456	40.0	37.5	41.0	32.0	41.0
52-53	37.377399420763446	40.0	37.0	41.0	32.0	41.0
54-55	37.21587216657731	40.0	37.0	41.0	31.0	41.0
56-57	37.053989803791424	40.0	36.0	41.0	31.0	41.0
58-59	36.850833290784124	40.0	36.0	41.0	31.0	41.0
60-61	36.6196552875901	39.0	35.0	41.0	31.0	41.0
62-63	36.352139551474615	39.0	35.0	41.0	30.5	41.0
64-65	36.07646465011786	39.0	35.0	41.0	30.0	41.0
66-67	35.77697194305214	38.0	35.0	40.0	30.0	41.0
68-69	35.46807639284833	37.0	35.0	40.0	30.0	41.0
70-71	35.118793134259775	37.0	35.0	39.5	29.5	41.0
72-73	34.75200016736022	36.0	34.0	39.0	29.0	41.0
74-75	34.38016511929379	36.0	34.0	39.0	29.0	41.0
76-77	34.050950194733545	35.0	34.0	38.5	29.0	40.0
78-79	33.68538601350795	35.0	34.0	37.0	28.5	39.0
80-81	33.329636870874495	35.0	34.0	37.0	28.0	39.0
82-83	33.113463491361095	35.0	34.0	36.5	28.0	39.0
84-85	32.847132041539375	35.0	34.0	36.0	28.0	37.0
86-87	32.59697850981327	35.0	34.0	36.0	27.5	37.0
88-89	32.24240000226929	35.0	34.0	35.0	27.0	37.0
90-91	32.056729263643405	35.0	34.0	35.0	26.5	36.0
92-93	31.88360327260143	35.0	33.0	35.0	26.0	36.0
94-95	31.714758263765376	35.0	33.0	35.0	26.0	36.0
96-97	31.54204606377275	35.0	33.0	35.0	25.0	36.0
98-99	31.349168375726528	35.0	33.0	35.0	25.0	35.0
100	31.18780546785691	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.16012853731773546
1101	2	0.20324489663465428
1101	3	0.1994836509938409
1101	4	0.0066759626765175994
1101	5	0.024418714425962662
1101	6	0.05888957474545009
1101	7	0.05400003538202469
1101	8	0.06558341667859224
1101	9	0.10926553128171435
1101	10-11	0.02222428979016655
1101	12-13	0.10857766880327091
1101	14-15	0.14925108842602697
1101	16-17	0.12190725346044928
1101	18-19	0.11941968000220982
1101	20-21	0.09773288793913792
1101	22-23	0.09796636582535712
1101	24-25	0.008625940200225557
1101	26-27	0.09546247403589092
1101	28-29	0.13382194635678957
1101	30-31	0.11841918648565297
1101	32-33	0.1211106215112423
1101	34-35	0.13070575089075476
1101	36-37	0.16297084464045497
1101	38-39	0.1634842720096401
1101	40-41	0.213475276658329
1101	42-43	0.21864290567074107
1101	44-45	0.0891960816979136
1101	46-47	0.11176034580645933
1101	48-49	0.0988882149770518
1101	50-51	0.022121374214549405
1101	52-53	0.07788478156587786
1101	54-55	0.07114746369219205
1101	56-57	0.2032025553050616
1101	58-59	0.20234385568402757
1101	60-61	0.1478759067337876
1101	62-63	0.1218280342250182
1101	64-65	0.13177445681773037
1101	66-67	0.12721459906573784
1101	68-69	0.12262990704247301
1101	70-71	0.13676999017869207
1101	72-73	0.2445093286514748
1101	74-75	0.21262369862841268
1101	76-77	0.14113020154896105
1101	78-79	0.08705473181651513
1101	80-81	0.1649868239114909
1101	82-83	0.1561593383216291
1101	84-85	0.08110767320916779
1101	86-87	0.1617006733143853
1101	88-89	0.355686028790295
1101	90-91	0.3393640113618943
1101	92-93	0.30174232267586376
1101	94-95	0.3141990877243508
1101	96-97	0.33636083160737584
1101	98-99	0.41155936898421075
1101	100	0.40134221958941296
1102	1	0.2533271953015763
1102	2	0.24291593560645452
1102	3	0.27658407158707377
1102	4	0.2335117400816813
1102	5	0.1221629299709619
1102	6	0.1586411715230085
1102	7	0.20062240011042576
1102	8	0.2549374971095588
1102	9	0.2650216602277595
1102	10-11	0.26355008100435384
1102	12-13	0.30016334845186776
1102	14-15	0.28506641405513733
1102	16-17	0.32975984016074733
1102	18-19	0.3426141847609827
1102	20-21	0.2968145869510863
1102	22-23	0.2637001951073614
1102	24-25	0.2862843193791562
1102	26-27	0.40877670518994336
1102	28-29	0.3735622754131782
1102	30-31	0.34080747312388837
1102	32-33	0.39812178597327375
1102	34-35	0.4009011588567759
1102	36-37	0.3439931010022761
1102	38-39	0.2934177126427855
1102	40-41	0.34431332876899035
1102	42-43	0.31844554420633386
1102	44-45	0.3419986922144247
1102	46-47	0.3067540016718908
1102	48-49	0.2963179636550137
1102	50-51	0.29838941247960094
1102	52-53	0.2601221779254743
1102	54-55	0.301987814804626
1102	56-57	0.2398562850794832
1102	58-59	0.25633640208517505
1102	60-61	0.26159844582709724
1102	62-63	0.3242005060823274
1102	64-65	0.30228629102730764
1102	66-67	0.2998844905613396
1102	68-69	0.33690003961977055
1102	70-71	0.3605170391127501
1102	72-73	0.37427067071465103
1102	74-75	0.3945636289777994
1102	76-77	0.3525579972510471
1102	78-79	0.38686712804711476
1102	80-81	0.36096545834754323
1102	82-83	0.3117402010390151
1102	84-85	0.3138252376414741
1102	86-87	0.3832134452162492
1102	88-89	0.4888798066203819
1102	90-91	0.4609452504102052
1102	92-93	0.45120810193655103
1102	94-95	0.43127460986716315
1102	96-97	0.45548824548575695
1102	98-99	0.4632900438006686
1102	100	0.3869475294475002
1103	1	0.05277956336029277
1103	2	0.054733000218874395
1103	3	0.08769092364854458
1103	4	0.08145234237665733
1103	5	-0.056883593832090185
1103	6	0.1055017550370394
1103	7	0.13885032597980285
1103	8	0.08482821603749358
1103	9	0.10623438682591058
1103	10-11	0.11908997027231294
1103	12-13	0.15658840445146893
1103	14-15	0.18212159923113802
1103	16-17	0.15901026482081448
1103	18-19	0.1253238180614531
1103	20-21	0.08259057751978816
1103	22-23	0.08150196048379144
1103	24-25	0.0730010056151329
1103	26-27	0.089780433805835
1103	28-29	0.11107116495554692
1103	30-31	0.1045738157301983
1103	32-33	0.04687578575229878
1103	34-35	0.02386979660838051
1103	36-37	0.07509226522206802
1103	38-39	0.05995013789630832
1103	40-41	0.12207226586909314
1103	42-43	0.08152823433477607
1103	44-45	0.059547433276463835
1103	46-47	0.034085977673498746
1103	48-49	0.058074995529437956
1103	50-51	0.010083780964428968
1103	52-53	-0.020108842731225707
1103	54-55	0.038988006049827106
1103	56-57	-0.005234364323186469
1103	58-59	-0.04028310280844494
1103	60-61	0.020191152431031867
1103	62-63	0.07629505943539527
1103	64-65	0.09844216734205702
1103	66-67	0.10227678613704683
1103	68-69	-6.77126425507879E-4
1103	70-71	0.07587796546724235
1103	72-73	0.08745452683031374
1103	74-75	0.07547363666754592
1103	76-77	-0.02492729502925073
1103	78-79	-0.091332852061349
1103	80-81	-0.02326701163973155
1103	82-83	0.04175838930100184
1103	84-85	0.04664022061608364
1103	86-87	0.017953805288435376
1103	88-89	-0.005893183674295699
1103	90-91	0.03532061802792441
1103	92-93	0.07569893614407164
1103	94-95	0.03734143767339049
1103	96-97	1.3956338834475446E-4
1103	98-99	-0.021888801525829393
1103	100	-0.02441938312909997
1104	1	0.12190785463617004
1104	2	0.1941560436667018
1104	3	0.12329992458803929
1104	4	0.13613393788603645
1104	5	0.011102606528730519
1104	6	0.0433397358295835
1104	7	0.040746921279684045
1104	8	0.053519570044635145
1104	9	0.15234831544886163
1104	10-11	0.08170651100297732
1104	12-13	0.048763392886421286
1104	14-15	0.039931524617465186
1104	16-17	0.10218890557687388
1104	18-19	0.0878673404655288
1104	20-21	0.07579352107146775
1104	22-23	0.09658887214267509
1104	24-25	0.06808346104890717
1104	26-27	0.05024236893380163
1104	28-29	0.14399623086748647
1104	30-31	0.10444060538281974
1104	32-33	0.09889457909986987
1104	34-35	0.048231990229162136
1104	36-37	0.13922293913694261
1104	38-39	0.07789194091576235
1104	40-41	0.10704505760983807
1104	42-43	0.07495270651092056
1104	44-45	0.062192919925443846
1104	46-47	-0.04137212605368745
1104	48-49	-0.04028191194317543
1104	50-51	0.005950564997895924
1104	52-53	-0.0018803717771262995
1104	54-55	-0.0685254179007444
1104	56-57	-0.06664555602013422
1104	58-59	-0.10517712515137845
1104	60-61	-0.053293990614648123
1104	62-63	-0.04995588711543064
1104	64-65	-0.09065373812244815
1104	66-67	-0.019223602133550344
1104	68-69	-0.2079345312045362
1104	70-71	-0.1431479718883395
1104	72-73	-0.08136729513788055
1104	74-75	-0.11123822501455294
1104	76-77	-0.21358930489182626
1104	78-79	-0.18103704202456328
1104	80-81	-0.1655862043984655
1104	82-83	-0.16647024167653512
1104	84-85	-0.0797659242349269
1104	86-87	-0.07812041675641979
1104	88-89	0.03007535083231261
1104	90-91	-4.060795367024639E-4
1104	92-93	-0.10664141513301928
1104	94-95	-0.009334588531853427
1104	96-97	0.1018145750938757
1104	98-99	0.04552158932102657
1104	100	0.12391440034648937
1105	1	-0.0597951204407714
1105	2	-0.023211646877019376
1105	3	-0.07623099693659796
1105	4	-0.06991686389160634
1105	5	-0.20886474289908818
1105	6	-0.13431120866196977
1105	7	-0.17661053508123103
1105	8	-0.15837883204653025
1105	9	-0.15776947999523827
1105	10-11	-0.22717938879873145
1105	12-13	-0.14419506585976904
1105	14-15	-0.12091762670409878
1105	16-17	-0.0964404373779999
1105	18-19	-0.16939831174659048
1105	20-21	-0.13800058693304607
1105	22-23	-0.13567943566343388
1105	24-25	-0.14527154757779925
1105	26-27	-0.1317418697188728
1105	28-29	-0.16233438758418117
1105	30-31	-0.07634764549300144
1105	32-33	-0.07246622489753207
1105	34-35	-0.1797638109326698
1105	36-37	-0.13169881841808717
1105	38-39	-0.18127938671536725
1105	40-41	-0.1924479797932861
1105	42-43	-0.24597370936180596
1105	44-45	-0.20565884746499563
1105	46-47	-0.11897502282148764
1105	48-49	-0.19376774518740092
1105	50-51	-0.12465075323542862
1105	52-53	-0.14101558685906213
1105	54-55	-0.1917369503425732
1105	56-57	-0.1385263976652169
1105	58-59	-0.11767800299723064
1105	60-61	-0.13612346689622257
1105	62-63	-0.10142862131147012
1105	64-65	-0.1689395901411075
1105	66-67	-0.1257595924019057
1105	68-69	-0.24527959361717677
1105	70-71	-0.168551948609462
1105	72-73	-0.2158930695743706
1105	74-75	-0.26482101831828686
1105	76-77	-0.1669967760570117
1105	78-79	-0.15530336581081627
1105	80-81	-0.185918084830746
1105	82-83	-0.1690131714266272
1105	84-85	-0.22358239569338423
1105	86-87	-0.10909734714385877
1105	88-89	-0.03812744902835874
1105	90-91	-0.08354628678242904
1105	92-93	-0.10784710773839734
1105	94-95	-0.03317352931510342
1105	96-97	0.03319482050957845
1105	98-99	0.08437698772893754
1105	100	0.15048727955031183
1106	1	0.1401455892092649
1106	2	0.15296493599404215
1106	3	0.09404680226218431
1106	4	0.09359316972609122
1106	5	0.12986427045537852
1106	6	0.17692125946104653
1106	7	0.08789256138592094
1106	8	0.1066535784025362
1106	9	0.09951185653182648
1106	10-11	0.07247173317741584
1106	12-13	0.024457215769167817
1106	14-15	0.084267534409733
1106	16-17	0.15528604889648534
1106	18-19	0.1650087190876448
1106	20-21	0.15092486920247694
1106	22-23	0.138196183239927
1106	24-25	0.13399049073732527
1106	26-27	0.2229565593787015
1106	28-29	0.2405748581071947
1106	30-31	0.24890131616818678
1106	32-33	0.24032589821894845
1106	34-35	0.25833990362684034
1106	36-37	0.22634931340269304
1106	38-39	0.21274253634614126
1106	40-41	0.18489390203663447
1106	42-43	0.23604732266070982
1106	44-45	0.15080847027302724
1106	46-47	0.14982510864548715
1106	48-49	0.22778972991474689
1106	50-51	0.24640926438105026
1106	52-53	0.1382813611471576
1106	54-55	0.18777255951750504
1106	56-57	0.16375035963082496
1106	58-59	0.25383839428672417
1106	60-61	0.14444670264925463
1106	62-63	0.23209331976613612
1106	64-65	0.19028197313731
1106	66-67	0.232940289881725
1106	68-69	0.18223520343348554
1106	70-71	0.2683143801628276
1106	72-73	0.29540585604901537
1106	74-75	0.22558454136993333
1106	76-77	0.20241510565278276
1106	78-79	0.2105782593554082
1106	80-81	0.23284198467882788
1106	82-83	0.2120307652819946
1106	84-85	0.2351885014329227
1106	86-87	0.25556813551180113
1106	88-89	0.2889428499972766
1106	90-91	0.26353290579569943
1106	92-93	0.27112034778626537
1106	94-95	0.28970482859588387
1106	96-97	0.2952965848408766
1106	98-99	0.31510824136513094
1106	100	0.3867313739474625
1107	1	0.11780447235974023
1107	2	0.11553397593966963
1107	3	0.14111656614697665
1107	4	0.05078451477523771
1107	5	-0.026145699208740325
1107	6	0.03762302893168368
1107	7	0.06879502035945961
1107	8	0.02072647179574716
1107	9	0.0379010908154811
1107	10-11	0.04134645389589764
1107	12-13	0.013795045835522046
1107	14-15	0.006569483795779263
1107	16-17	-0.0066446240450446226
1107	18-19	0.07727743553984112
1107	20-21	-0.02127910751567441
1107	22-23	0.033878461874635946
1107	24-25	0.01602578163728907
1107	26-27	0.03400503543383593
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2216	10-11	-0.16064555803773573
2216	12-13	-0.23260118171551625
2216	14-15	-0.2934830511235873
2216	16-17	-0.2795132971846499
2216	18-19	-0.32361374289938993
2216	20-21	-0.3498302751478235
2216	22-23	-0.28275241885367564
2216	24-25	-0.32122128047991083
2216	26-27	-0.3607196506474182
2216	28-29	-0.3228820654908162
2216	30-31	-0.29812220671007594
2216	32-33	-0.3071550733009829
2216	34-35	-0.3345139124166465
2216	36-37	-0.2799304355814485
2216	38-39	-0.2616345048563744
2216	40-41	-0.25038013847408536
2216	42-43	-0.30773441558860526
2216	44-45	-0.3499834948932161
2216	46-47	-0.20112968687405441
2216	48-49	-0.20967553735073352
2216	50-51	-0.20862513167239882
2216	52-53	-0.2457001299109578
2216	54-55	-0.32323333529513576
2216	56-57	-0.31423330990205045
2216	58-59	-0.30009193724743
2216	60-61	-0.2572246141845227
2216	62-63	-0.24473363500980838
2216	64-65	-0.18932388743785822
2216	66-67	-0.19602880350179674
2216	68-69	-0.17981844475414022
2216	70-71	-0.1997403975415537
2216	72-73	-0.16772112009947193
2216	74-75	-0.23110354458101057
2216	76-77	-0.3075517833755299
2216	78-79	-0.2968816144251818
2216	80-81	-0.25894650281428255
2216	82-83	-0.20750899945817025
2216	84-85	-0.23879913317245638
2216	86-87	-0.28974892488741233
2216	88-89	-0.19867326262237128
2216	90-91	-0.23114285127556755
2216	92-93	-0.2524720547689192
2216	94-95	-0.21687301606742082
2216	96-97	-0.2406382493261674
2216	98-99	-0.22012015074489
2216	100	-0.21323740011840897
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28769.0
3	5053.0
4	3367.0
5	2925.0
6	2514.0
7	2892.0
8	3269.0
9	3503.0
10	3829.0
11	4002.0
12	4611.0
13	4905.0
14	5364.0
15	5579.0
16	5570.0
17	5672.0
18	5720.0
19	5548.0
20	5754.0
21	6003.0
22	6958.0
23	9461.0
24	14863.0
25	20011.0
26	21212.0
27	18294.0
28	19275.0
29	23718.0
30	29933.0
31	36843.0
32	47643.0
33	65801.0
34	92440.0
35	142603.0
36	268923.0
37	577481.0
38	918953.0
39	390196.0
40	807.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.200516832084	19.568291526308883	13.376872262289435	23.854319379317683
2	15.251503168573208	19.890531484623253	40.889415712331676	23.968549634471863
3	18.368932727788817	25.474560034061877	29.62024552937837	26.536261708770937
4	12.186760965161984	16.55367093358135	38.83250630601678	32.42706179523989
5	12.887107181611759	38.36820539343651	35.580285198503454	13.16440222644828
6	31.121996125508556	38.09210906590572	17.802766896112495	12.98312791247323
7	27.63591348571274	32.45966583760806	22.439900342757934	17.464520333921264
8	25.06550943091109	37.17389353870792	20.01271001761458	17.7478870127664
9	25.09421924544455	17.241155519650125	20.758307553888585	36.906317681016745
10-11	24.045708193814914	27.12112181941891	28.69263578586072	20.14053420090546
12-13	24.541426704785135	24.409892809982818	28.742035576567247	22.3066449086648
14-15	21.844841560943397	26.24634120089292	25.72365181442922	26.185165423734464
16-17	21.792595006818974	29.66382482142195	26.283329943499417	22.260250228259654
18-19	21.73353782219364	28.79147119024681	28.03119891435779	21.443792073201763
20-21	22.85485376282039	28.004626929431524	27.5378021659605	21.60271714178758
22-23	22.51218478134242	27.798660795582396	27.57024102877908	22.1189133942961
24-25	22.286905457514976	27.91556461530686	27.59118689933952	22.20634302783864
26-27	22.063244092477596	27.880633114752936	27.665398214710024	22.390724578059444
28-29	22.099050154926566	27.896268534178713	27.74822207713311	22.256459233761614
30-31	22.293112449001594	28.249942546997975	27.37529152078806	22.081653483212374
32-33	22.013162248690318	27.988841596564594	27.788084675052556	22.209911479692536
34-35	22.14876821453212	27.7695158519336	27.872766775438016	22.20894915809626
36-37	22.430716017231276	28.02871856192296	27.354371720730725	22.186193700115037
38-39	22.234537537073756	28.229922810695683	27.48326128844593	22.052278363784627
40-41	22.03354609929078	27.949343971631208	27.789237588652483	22.22787234042553
42-43	22.04973569401995	27.879697605732147	27.725727448708824	22.34483925153908
44-45	22.05076323216183	28.02146159938596	27.73123249232672	22.196542676125485
46-47	22.158186158245734	28.029782000198228	27.683331232288506	22.12870060926754
48-49	22.115247551715555	28.011838427764886	27.585369935797466	22.287544084722093
50-51	22.096855808451433	27.980056657786335	27.57682470305015	22.346262830712078
52-53	22.167359381687575	27.95134364019527	27.606705949477018	22.27459102864014
54-55	22.194458007422938	28.216792775772586	27.48744139108999	22.101307825714485
56-57	22.00544396234507	28.025679843740942	27.559363496447137	22.409512697466855
58-59	21.99276360555977	27.646303137839347	27.854551592109605	22.506381664491283
60-61	22.158441568572666	28.01692026258001	27.570351280366527	22.254286888480795
62-63	22.03463031542578	28.03907044494906	27.533909086911933	22.392390152713222
64-65	22.0693510360779	27.842109238235686	27.609643702953296	22.478896022733117
66-67	21.89661564056891	27.876638145877447	27.554536014610186	22.67221019894345
68-69	22.179320977585764	28.005986868886012	27.517576942115586	22.29711521141264
70-71	22.127644712100512	27.781688055788894	27.646786419275493	22.443880812835104
72-73	21.978278953299288	27.894478929613143	27.688856235732466	22.438385881355103
74-75	22.011532146527724	27.958106569259982	27.568914796396786	22.461446487815508
76-77	22.048137404141656	27.871530758530405	27.49579193079016	22.58453990653778
78-79	22.10907365684391	27.953333574452184	27.5757875544379	22.36180521426601
80-81	22.123069465810854	27.738313077768495	27.702836511892983	22.435780944527664
82-83	21.9346898309484	27.869171875509714	27.764835451619813	22.431302841922076
84-85	21.9588388121188	27.78993617785525	27.628403992509696	22.62282101751625
86-87	22.054547404756562	27.827792813117476	27.669347024997275	22.448312757128686
88-89	21.876831045216125	27.734909333552604	27.739903599956406	22.648356021274864
90-91	21.833792855430353	27.65261627906977	27.842597270267493	22.670993595232385
92-93	21.98364836237461	27.81499752766835	27.68299713666179	22.51835697329525
94-95	21.804627142069066	27.563290704640643	27.859021575101746	22.77306057818854
96-97	21.85806559313763	27.563988693987152	27.821473719655398	22.75647199321982
98-99	21.780434831460273	27.690962406942976	27.788625240456383	22.739977521140364
100	21.911563592159247	27.499602283711894	27.699994221521195	22.888839902607664
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	819.0
1	743.0
2	611.0
3	496.5
4	383.5
5	301.5
6	239.5
7	211.0
8	221.5
9	232.0
10	236.5
11	232.0
12	243.5
13	271.0
14	298.0
15	342.0
16	420.0
17	551.5
18	769.5
19	1061.0
20	1515.5
21	2272.0
22	3352.5
23	5253.5
24	7747.0
25	10828.5
26	15018.5
27	20331.0
28	27320.5
29	35284.5
30	42855.5
31	49922.5
32	56944.0
33	64679.0
34	72799.5
35	79478.0
36	84618.5
37	90519.5
38	96045.5
39	98492.5
40	100167.0
41	102188.0
42	103008.5
43	103422.5
44	102545.0
45	100959.5
46	99780.5
47	96990.5
48	93901.0
49	92192.0
50	91151.5
51	89018.5
52	83085.0
53	77510.5
54	74761.0
55	71133.5
56	65219.5
57	58708.0
58	52794.5
59	47824.0
60	43037.0
61	37022.5
62	31866.5
63	27326.5
64	22545.0
65	18199.0
66	14491.0
67	11384.5
68	8912.5
69	6932.0
70	5123.0
71	3745.0
72	2818.0
73	2074.5
74	1559.0
75	1118.0
76	679.5
77	454.0
78	341.0
79	216.0
80	156.0
81	100.5
82	53.5
83	43.0
84	35.0
85	24.5
86	22.5
87	17.0
88	7.5
89	6.5
90	7.0
91	5.5
92	4.5
93	5.5
94	3.5
95	2.0
96	2.5
97	2.0
98	1.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.329508159519818
2	0.11988239398864786
3	0.06609310334068016
4	0.04010262869008008
5	0.044144803465207515
6	0.009963606243954468
7	0.007020619346273966
8	0.015636833998519287
9	0.0181188711411414
10-11	0.021079586875554914
12-13	0.016257343284174816
14-15	0.01845571903906868
16-17	0.019803110630777828
18-19	0.019519449243049585
20-21	0.024235319814031594
22-23	0.016150970263776727
24-25	0.025228134671080437
26-27	0.019643551100180692
28-29	0.0191826013451223
30-31	0.020139958528705114
32-33	0.02428850632423064
34-35	0.015406359120990091
36-37	0.009786317876624316
38-39	0.013420729406892403
40-41	0.009360825795031953
42-43	0.00840346861144914
44-45	0.008882147203240548
46-47	0.008456655121648185
48-49	0.004467666856719796
50-51	0.006205092856555273
52-53	0.007126992366672057
54-55	0.003084817591544621
56-57	0.0026770543466852746
58-59	0.00265932550995226
60-61	0.0033684789792728623
62-63	0.00269478318341829
64-65	0.002162918081427838
66-67	0.0014360357753742203
68-69	0.003262105958874772
70-71	0.002162918081427838
72-73	0.0017728836733015066
74-75	0.002145189244694823
76-77	0.003084817591544621
78-79	0.00276569853035035
80-81	0.0032798347956077873
82-83	0.002127460407961808
84-85	0.002942986897680501
86-87	0.0020033585508307025
88-89	0.6045356037590808
90-91	0.6152615499825548
92-93	0.6113612059012915
94-95	0.5981886802086613
96-97	0.5956889142293061
98-99	0.5935969114948104
100	0.5941996919437329
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2820264.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.88067696123509
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.7064061209465	44.73470171863234
2	14.364287472110288	17.20286515791505
3	4.848376115859695	8.709721319410866
4	2.1835623449829122	5.23012765618555
5	1.1545083727429624	3.456637145863126
6	0.6970874660894659	2.5045241622377543
7	0.44343807866686474	1.85873606386729
8	0.29871916098479667	1.4310004464849433
9	0.21325145257188485	1.1492677208674065
>10	1.027558883203788	10.586237477464694
>50	0.050039973701705494	1.9966827150873234
>100	0.01270534524946583	1.0974817771066567
>500	0.0	0.0
>1k	5.921288973411286E-5	0.04201663887699931
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.091534693206026E-5	0.0	0.0	0.0
3	0.0	7.091534693206026E-5	0.0	3.545767346603013E-5	0.0
4	0.0	7.091534693206026E-5	0.0	3.545767346603013E-5	0.0
5	0.0	7.091534693206026E-5	0.0	3.545767346603013E-5	0.0
6	0.0	7.091534693206026E-5	0.0	3.545767346603013E-5	0.0
7	0.0	7.091534693206026E-5	0.0	3.545767346603013E-5	0.0
8	0.0	7.091534693206026E-5	0.0	3.545767346603013E-5	0.0
9	0.0	7.091534693206026E-5	0.0	1.4183069386412053E-4	0.0
10-11	0.0	7.091534693206026E-5	0.0	1.595595305971356E-4	0.0
12-13	0.0	7.091534693206026E-5	0.0	1.7728836733015065E-4	0.0
14-15	0.0	7.091534693206026E-5	0.0	1.7728836733015065E-4	0.0
16-17	0.0	7.091534693206026E-5	0.0	1.7728836733015065E-4	0.0
18-19	0.0	7.091534693206026E-5	0.0	1.7728836733015065E-4	0.0
20-21	0.0	7.091534693206026E-5	0.0	1.9501720406316572E-4	0.0
22-23	0.0	7.091534693206026E-5	0.0	3.191190611942712E-4	0.0
24-25	0.0	7.091534693206026E-5	0.0	5.141362652574369E-4	0.0
26-27	0.0	7.091534693206026E-5	0.0	7.268823060536177E-4	0.0
28-29	0.0	7.091534693206026E-5	0.0	0.0014183069386412054	0.0
30-31	0.0	7.091534693206026E-5	0.0	0.004396751509787736	0.0
32-33	0.0	7.091534693206026E-5	0.0	0.011807405264188033	0.0
34-35	0.0	7.091534693206026E-5	0.0	0.02237379195706501	0.0
36-37	0.0	7.091534693206026E-5	0.0	0.042442835138838064	0.0
38-39	0.0	7.091534693206026E-5	0.0	0.07717362629881458	0.0
40-41	0.0	7.091534693206026E-5	0.0	0.11878320611120094	0.0
42-43	0.0	7.091534693206026E-5	0.0	0.15916949618900927	0.0
44-45	0.0	7.091534693206026E-5	0.0	0.20989169808216535	0.0
46-47	0.0	7.091534693206026E-5	0.0	0.2679359095460567	0.0
48-49	0.0	7.091534693206026E-5	0.0	0.3345963356621933	0.0
50-51	0.0	7.091534693206026E-5	0.0	0.40643358210437036	0.0
52-53	0.0	7.091534693206026E-5	0.0	0.4814620191584902	0.0
54-55	0.0	7.091534693206026E-5	0.0	0.5609935807427957	0.0
56-57	0.0	7.091534693206026E-5	0.0	0.6547968558971784	0.0
58-59	0.0	7.091534693206026E-5	0.0	0.7632796078664976	0.0
60-61	0.0	7.091534693206026E-5	0.0	0.8809636261002516	0.0
62-63	0.0	7.091534693206026E-5	0.0	0.9994454419869914	0.0
64-65	0.0	7.091534693206026E-5	0.0	1.1226253996079798	0.0
66-67	0.0	7.091534693206026E-5	0.0	1.2678245724513735	0.0
68-69	0.0	7.091534693206026E-5	0.0	1.4336778400887291	0.0
70-71	0.0	7.091534693206026E-5	0.0	1.6115689878678023	0.0
72-73	0.0	7.091534693206026E-5	0.0	1.7977572312379266	0.0
74-75	0.0	7.091534693206026E-5	0.0	1.9820839467510842	0.0
76-77	0.0	1.063730203980904E-4	0.0	2.1831466841402083	0.0
78-79	0.0	1.063730203980904E-4	0.0	2.412132339383831	0.0
80-81	0.0	1.063730203980904E-4	0.0	2.664271855400771	0.0
82-83	0.0	1.063730203980904E-4	0.0	2.9285024380696276	0.0
84-85	0.0	1.063730203980904E-4	0.0	3.1979630275747235	0.0
86-87	0.0	1.063730203980904E-4	0.0	3.4779545460992303	0.0
88	0.0	1.063730203980904E-4	0.0	3.7075607106285084	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2320	0.0	36.766968	1
GTATCAA	3520	0.0	33.604565	1
TCAACGC	3640	0.0	31.353271	4
ATCAACG	3720	0.0	30.559263	3
CAACGCA	3880	0.0	29.535463	5
AACGCAG	4000	0.0	28.525408	6
GTACATG	8695	0.0	28.238092	1
TACATGG	8660	0.0	26.596596	2
TATCAAC	4525	0.0	25.554325	2
ACATGGG	8935	0.0	24.86778	3
ACGCAGA	4935	0.0	22.264568	7
CATGGGG	6075	0.0	21.491985	4
CGCAGAG	5140	0.0	21.285608	8
ACCGTCC	215	1.2059772E-6	19.6561	8
GAGTACA	6085	0.0	19.516735	1
TATACCG	145	6.63518E-4	19.434303	5
AGTACAT	6180	0.0	17.189682	2
GCAGAGT	6285	0.0	16.36184	9
ATGGGGG	3245	0.0	15.920755	5
ACACCGT	590	0.0	15.917086	6
>>END_MODULE