FastQCFastQC Report
Wed 25 May 2016
SRR1033174_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033174_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2820264
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58660.20799471255173274No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46930.1664028615760794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40500.052.2838061
GTATCAA74100.042.800751
TCAACGC88350.035.7052964
ATCAACG88800.035.5772973
CAACGCA90550.034.839045
AACGCAG94900.033.3299456
TATCAAC95250.033.31622
ACGCAGA108500.029.0655467
CGCAGAG112500.027.9470678
GTACATG88400.025.6569161
TACATGG88650.024.9250352
GCAGAGT125250.024.6510189
GCGTACG1000.001747287723.5277121
GTGGTAT19950.023.3508131
ACATGGG90950.023.3125883
TGGTATC19000.022.0218122
GAGTACT77700.021.19819512-13
CATGGGG62600.020.7277154
AGAGTAC114800.020.30425310-11
CAGAGTA123300.020.08606110-11
Produced by FastQC (version 0.11.5)