FastQCFastQC Report
Wed 25 May 2016
SRR1033173_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033173_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879649
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76850.8736439193360078No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32390.36821504941175404No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17580.19985244114413817No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12920.14687676561901394No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT11090.1260730132132248No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC10210.11606902298530437No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9730.11061230104280229No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9670.10993021079998955No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG651.4157566E-436.1428225
GAGAACA7750.034.024481
CATGGGT8250.031.8933014
GTACATG75000.029.3825681
GGCGTCG650.00617627928.9011049
TACATGG74150.028.5307922
GTACAGG3800.028.5002981
ACGTGCG856.8451476E-427.6260579
TACATGA2900.027.5589962
GTACATA4800.027.4676781
CGTATCA909.28238E-426.22247594
GTACAAA5300.025.764831
ACATGGG78650.025.3370513
ATATATC2251.1641532E-1025.066153
CCATATG1501.1697321E-625.0476238
AGTACGG2552.1827873E-1123.9534765
GAACAAA13050.023.814341
TATACGG1000.001755652623.5062052
ACTAGGG1201.8378228E-423.4995173
GCAGCGA500.001767136623.4814838-39
Produced by FastQC (version 0.11.5)