##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033173_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	879649
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.419066013830516	33.0	31.0	34.0	25.0	34.0
2	30.508652883138616	33.0	31.0	34.0	25.0	34.0
3	30.622625615444342	34.0	31.0	34.0	25.0	34.0
4	33.600723697747625	37.0	35.0	37.0	25.0	37.0
5	34.0928290715956	37.0	35.0	37.0	30.0	37.0
6	33.952155916734974	37.0	35.0	37.0	29.0	37.0
7	34.02095835952749	37.0	35.0	37.0	30.0	37.0
8	33.871324812510444	37.0	35.0	37.0	28.0	37.0
9	35.21326460895198	39.0	37.0	39.0	25.0	39.0
10-11	35.36124067667899	39.0	37.0	39.0	27.0	39.0
12-13	35.32684911822784	39.0	37.0	39.0	27.0	39.0
14-15	36.68934995663043	41.0	38.0	41.0	27.0	41.0
16-17	36.76248139883067	40.5	38.0	41.0	28.0	41.0
18-19	36.80125993436018	40.5	38.0	41.0	29.0	41.0
20-21	36.879970306338095	40.0	38.0	41.0	29.0	41.0
22-23	36.90530256954763	40.0	38.0	41.0	29.0	41.0
24-25	36.960514364252106	40.0	38.0	41.0	29.5	41.0
26-27	36.88295729319308	40.0	37.5	41.0	30.0	41.0
28-29	36.76594698567269	40.0	37.0	41.0	30.0	41.0
30-31	36.66185489894265	40.0	37.0	41.0	30.0	41.0
32-33	36.62184519052485	40.0	37.0	41.0	30.0	41.0
34-35	36.46993971459071	40.0	37.0	41.0	29.0	41.0
36-37	36.285768528128834	40.0	37.0	41.0	28.0	41.0
38-39	36.09897015741506	40.0	36.0	41.0	27.0	41.0
40-41	35.96920874121382	40.0	36.0	41.0	27.0	41.0
42-43	35.76984172095916	40.0	35.0	41.0	26.5	41.0
44-45	35.6167459975513	40.0	35.0	41.0	26.0	41.0
46-47	35.43377983718506	40.0	35.0	41.0	26.0	41.0
48-49	35.247223040098945	40.0	35.0	41.0	26.0	41.0
50-51	35.06268750376571	39.5	35.0	41.0	26.0	41.0
52-53	34.88451643780644	39.0	35.0	41.0	25.5	41.0
54-55	34.65227380466527	39.0	35.0	41.0	25.0	41.0
56-57	34.40891082693211	38.5	34.5	41.0	24.5	41.0
58-59	34.12243235654221	37.5	34.0	40.5	24.0	41.0
60-61	33.796704708355264	37.0	34.0	40.0	23.0	41.0
62-63	33.46108902528168	36.5	34.0	40.0	22.0	41.0
64-65	33.12114093234915	36.0	33.5	39.0	20.5	41.0
66-67	32.75182260196965	35.0	33.0	39.0	19.5	41.0
68-69	32.386616707345766	35.0	33.0	38.5	18.0	40.5
70-71	32.007013024513185	35.0	33.0	37.0	14.0	40.0
72-73	31.65156613603835	35.0	33.0	37.0	8.5	39.0
74-75	31.316498967201692	35.0	32.5	36.0	4.5	39.0
76-77	31.027900901382253	35.0	32.0	36.0	2.0	38.5
78-79	30.711050657705517	35.0	32.0	35.0	2.0	37.0
80-81	30.391523778234273	35.0	31.0	35.0	2.0	37.0
82-83	30.253680161064242	35.0	31.5	35.0	2.0	36.0
84-85	30.041944571073234	35.0	31.0	35.0	2.0	36.0
86-87	29.864646580624772	35.0	31.0	35.0	2.0	36.0
88-89	29.55856881551619	35.0	31.0	35.0	2.0	35.5
90-91	29.3634966901571	35.0	31.0	35.0	2.0	35.0
92-93	29.161237038864364	35.0	31.0	35.0	2.0	35.0
94-95	28.924260131029534	35.0	30.0	35.0	2.0	35.0
96-97	28.709037923080682	35.0	30.0	35.0	2.0	35.0
98-99	28.46832031867256	35.0	29.5	35.0	2.0	35.0
100	28.277948363494986	35.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.48221843956126165
1101	2	0.4547491367910119
1101	3	0.3811550855618613
1101	4	0.3745427387544211
1101	5	0.33615588202456337
1101	6	0.2316835341311645
1101	7	0.19757196175832092
1101	8	0.19819218745090694
1101	9	0.30919360656313444
1101	10-11	0.34423681160898667
1101	12-13	0.3014232682682234
1101	14-15	0.3678347022755517
1101	16-17	0.22633685155485495
1101	18-19	0.23157848605628573
1101	20-21	0.27894421638926303
1101	22-23	0.17807754306845425
1101	24-25	0.14951119985985173
1101	26-27	0.23501450805895274
1101	28-29	0.2775343108067929
1101	30-31	0.2529570084704531
1101	32-33	0.32599923481505755
1101	34-35	0.2757119984105998
1101	36-37	0.27196346080067
1101	38-39	0.30691571153758446
1101	40-41	0.38096489698638436
1101	42-43	0.36155105095105
1101	44-45	0.2468597382647033
1101	46-47	0.3383564783879578
1101	48-49	0.3853314858481198
1101	50-51	0.3054846146649908
1101	52-53	0.22653371653677112
1101	54-55	0.16747826030674418
1101	56-57	0.23057758758660185
1101	58-59	0.2609861582337345
1101	60-61	0.26178937520322165
1101	62-63	0.2641081488174635
1101	64-65	0.12582204005988018
1101	66-67	0.22391500278280319
1101	68-69	0.18655998447107436
1101	70-71	0.22814599632452826
1101	72-73	0.3114898571269933
1101	74-75	0.31274048634625373
1101	76-77	0.34018139228302857
1101	78-79	0.27177851101166084
1101	80-81	0.3734404850303825
1101	82-83	0.4430535423524695
1101	84-85	0.43885445096119113
1101	86-87	0.5406102283298289
1101	88-89	0.5811470557773433
1101	90-91	0.6112268756645918
1101	92-93	0.6844095366040079
1101	94-95	0.7978877164173639
1101	96-97	0.7849741451145427
1101	98-99	0.7868939331646487
1101	100	0.6678718901149701
1102	1	0.10021096655817274
1102	2	0.2067898061121589
1102	3	0.1769923653642138
1102	4	0.07699506694125091
1102	5	0.028227847228201597
1102	6	0.16447938137292795
1102	7	-0.18652215884577572
1102	8	0.1355069332983021
1102	9	-0.06510943934464564
1102	10-11	-0.05355883639356307
1102	12-13	0.0894292975533233
1102	14-15	0.2333562798822868
1102	16-17	0.17930413647612653
1102	18-19	-0.04323062745182682
1102	20-21	0.031807896919914924
1102	22-23	0.07000444679913187
1102	24-25	0.11985789141609615
1102	26-27	0.13865629434577187
1102	28-29	0.14450918520945777
1102	30-31	0.08911685655821344
1102	32-33	0.16735194646074092
1102	34-35	0.1993259831642078
1102	36-37	0.06311325497123477
1102	38-39	0.15032939957801972
1102	40-41	0.18988989415105806
1102	42-43	0.11669908568610765
1102	44-45	0.026448293964328684
1102	46-47	-0.03018395644396321
1102	48-49	-0.055273772227963036
1102	50-51	-0.0374815514514637
1102	52-53	0.016441330846440394
1102	54-55	0.03266228070884125
1102	56-57	0.060528068979188276
1102	58-59	0.02715863502236715
1102	60-61	-0.01698957961852443
1102	62-63	0.05478704877179297
1102	64-65	0.08428066420583491
1102	66-67	0.07090886621504922
1102	68-69	-0.003341039618476316
1102	70-71	-0.04880340611109801
1102	72-73	-0.0071993940707422155
1102	74-75	-0.036895595568648076
1102	76-77	-0.054980083435840044
1102	78-79	-0.08846514321941612
1102	80-81	0.1328070269426469
1102	82-83	0.0455130738031464
1102	84-85	0.1250178240504063
1102	86-87	0.053671508181228234
1102	88-89	0.11792048400984356
1102	90-91	0.15402192594950748
1102	92-93	0.25933727407416285
1102	94-95	-0.049027600786789804
1102	96-97	0.08711887860910394
1102	98-99	0.3005732377278427
1102	100	0.3297177341382529
1103	1	0.3951729068341372
1103	2	0.32246616841155173
1103	3	0.31097541440087895
1103	4	0.3273927580853595
1103	5	0.17802349402982998
1103	6	0.15612620953277911
1103	7	0.2483430682489498
1103	8	0.1433060268793227
1103	9	0.3677222026500644
1103	10-11	0.2968219818886695
1103	12-13	0.2499904196796905
1103	14-15	0.3062592005083715
1103	16-17	0.35848435116671595
1103	18-19	0.36050822190740206
1103	20-21	0.250110678584349
1103	22-23	0.29807433139317396
1103	24-25	0.4207816990242108
1103	26-27	0.4870031591248889
1103	28-29	0.4534435497767433
1103	30-31	0.4902393071166742
1103	32-33	0.6029042453329225
1103	34-35	0.7109815203732879
1103	36-37	0.7498059456327297
1103	38-39	0.6700500174047477
1103	40-41	0.5570864718336352
1103	42-43	0.648287440906266
1103	44-45	0.596051796446254
1103	46-47	0.7288520505380092
1103	48-49	0.6406566261115643
1103	50-51	0.650194407078132
1103	52-53	0.4807986995346809
1103	54-55	0.6268345857849198
1103	56-57	0.5527462903437694
1103	58-59	0.5586806776849187
1103	60-61	0.532942671051785
1103	62-63	0.557822321894804
1103	64-65	0.7134962087535044
1103	66-67	0.6852169794091125
1103	68-69	0.5673109380798778
1103	70-71	0.5748798291164903
1103	72-73	0.5270952503060613
1103	74-75	0.5010340821744244
1103	76-77	0.5834197503406813
1103	78-79	0.5227800666193048
1103	80-81	0.5098727552129425
1103	82-83	0.60595549645139
1103	84-85	0.5371275285984929
1103	86-87	0.5988347807501526
1103	88-89	0.7083111312867203
1103	90-91	0.7737087243718577
1103	92-93	0.7586494654905174
1103	94-95	0.7444673583536243
1103	96-97	0.8234350981449339
1103	98-99	0.8214620617777832
1103	100	1.0134002030874
1104	1	0.1692397211714045
1104	2	0.1729121543960659
1104	3	0.34632500849556536
1104	4	0.09847689067165533
1104	5	0.07522931889854334
1104	6	0.12847479523743033
1104	7	5.432563306868587E-4
1104	8	0.13193756229477316
1104	9	0.12032815441729383
1104	10-11	0.14335479138792095
1104	12-13	0.013757705354322525
1104	14-15	-0.017677953565701898
1104	16-17	-0.011331362722444283
1104	18-19	0.1116352252328241
1104	20-21	0.026914450416271052
1104	22-23	0.17527593575304934
1104	24-25	0.16229960927748976
1104	26-27	0.2726299896643809
1104	28-29	0.3591491678688499
1104	30-31	0.3695256728903402
1104	32-33	0.37410380458716475
1104	34-35	0.2952893710862412
1104	36-37	0.4433343335116575
1104	38-39	0.27207090467467765
1104	40-41	0.1254633179521889
1104	42-43	0.1886733200467532
1104	44-45	0.21423029281208272
1104	46-47	0.22377044459190643
1104	48-49	0.014625221560223167
1104	50-51	0.08741292179892923
1104	52-53	0.015529380084650768
1104	54-55	0.034070492401205854
1104	56-57	-0.0409883076315154
1104	58-59	-0.16717956965388936
1104	60-61	-0.18835468220763119
1104	62-63	-0.15234591778478546
1104	64-65	-0.12184623313660836
1104	66-67	-0.05694392111718116
1104	68-69	-0.07956914281793814
1104	70-71	-0.026915161791002618
1104	72-73	-0.04390772954312894
1104	74-75	0.05276399510164609
1104	76-77	0.0292088721915853
1104	78-79	0.07907889280798841
1104	80-81	0.2153941863336719
1104	82-83	0.29363890446979113
1104	84-85	0.3246808514702515
1104	86-87	0.17467789112354026
1104	88-89	0.4073796451577465
1104	90-91	0.44871892157442517
1104	92-93	0.31984117856389815
1104	94-95	0.3755888665198839
1104	96-97	0.4925847364274958
1104	98-99	0.4448400692444032
1104	100	0.49275228416648886
1105	1	0.33481066561614625
1105	2	0.24813486796820783
1105	3	0.3244852926695181
1105	4	0.3330917193267524
1105	5	0.3956939390273533
1105	6	0.5110710144603985
1105	7	0.36876949798561753
1105	8	0.3999473809652372
1105	9	0.31347730291263787
1105	10-11	0.2856927217578118
1105	12-13	0.45087759324800913
1105	14-15	0.4899232847431776
1105	16-17	0.42092570013524977
1105	18-19	0.40682740937840123
1105	20-21	0.3795132661796856
1105	22-23	0.44597866131745434
1105	24-25	0.3843359394289507
1105	26-27	0.5673677792321783
1105	28-29	0.5593196829475175
1105	30-31	0.5423629153108394
1105	32-33	0.44081682530059396
1105	34-35	0.4761574855695798
1105	36-37	0.45261663099488914
1105	38-39	0.4939084790240713
1105	40-41	0.43778932297394846
1105	42-43	0.4411166110680824
1105	44-45	0.4550915411859009
1105	46-47	0.5593264522369807
1105	48-49	0.48203830437647355
1105	50-51	0.6694589153637907
1105	52-53	0.5454799387423748
1105	54-55	0.5497039411958227
1105	56-57	0.4763158275599082
1105	58-59	0.4536485661371401
1105	60-61	0.5285016166782626
1105	62-63	0.5769844826178172
1105	64-65	0.6321814160038599
1105	66-67	0.5648043804241496
1105	68-69	0.4165260083435456
1105	70-71	0.422954208133028
1105	72-73	0.43367301751099774
1105	74-75	0.5051238354545582
1105	76-77	0.4484551259458627
1105	78-79	0.4620067063067843
1105	80-81	0.3688647838591983
1105	82-83	0.31895149314030746
1105	84-85	0.42993643670905257
1105	86-87	0.44216037116765605
1105	88-89	0.46095470659135884
1105	90-91	0.3340395006297108
1105	92-93	0.3435018187583445
1105	94-95	0.46342855825431784
1105	96-97	0.4724541866569041
1105	98-99	0.36826186787064685
1105	100	0.41026394183003134
1106	1	-0.12455409305119147
1106	2	-0.2029832629041124
1106	3	-0.12127542620275023
1106	4	-0.03741735314716266
1106	5	-0.3579217846841445
1106	6	-0.21695219891557116
1106	7	-0.1740057186588544
1106	8	-0.31055929991810416
1106	9	-0.06931918892293965
1106	10-11	-0.2879059867832865
1106	12-13	-0.3023902910538112
1106	14-15	-0.24977917398631888
1106	16-17	-0.22908868677676963
1106	18-19	-0.19134426945589667
1106	20-21	-0.11848626795840289
1106	22-23	-0.1757990752164602
1106	24-25	-0.20782406545388454
1106	26-27	-0.12662727316483569
1106	28-29	-0.12857774771192965
1106	30-31	-0.1865029600549022
1106	32-33	-0.18554603877858256
1106	34-35	-0.3334485497088693
1106	36-37	-0.19166385524537333
1106	38-39	-0.10801843297162605
1106	40-41	-0.0692459392851319
1106	42-43	-0.06059580514840235
1106	44-45	-0.018552694769418565
1106	46-47	-0.036405679654251344
1106	48-49	0.048765238295082725
1106	50-51	-0.02540838250009614
1106	52-53	-0.05614864724216062
1106	54-55	-0.10538883881645944
1106	56-57	-0.022751514398933637
1106	58-59	-0.12246100109707214
1106	60-61	-0.04315485874005276
1106	62-63	-0.02662588492729867
1106	64-65	-0.004533173819673664
1106	66-67	-0.007405150313388731
1106	68-69	-0.12799183080453957
1106	70-71	-0.07959682180794303
1106	72-73	-0.1139607402881353
1106	74-75	-0.08311558020014687
1106	76-77	-0.09623118331918334
1106	78-79	-0.17732742256257694
1106	80-81	-0.03753122617053606
1106	82-83	-0.01351665323998219
1106	84-85	-0.07590614431542164
1106	86-87	-0.13344004885543725
1106	88-89	-0.14119610561521867
1106	90-91	-0.12138255905292894
1106	92-93	-0.050047799895065026
1106	94-95	0.12143640067839456
1106	96-97	-0.00530682369046076
1106	98-99	-0.08319911993099893
1106	100	-0.07149582040600677
1107	1	0.2619599248187612
1107	2	0.2657790278920764
1107	3	0.27440954999070044
1107	4	0.28647780549376023
1107	5	0.21816778716814156
1107	6	0.08538866274732726
1107	7	0.20988587709714324
1107	8	0.2012005338756282
1107	9	0.4035103432286249
1107	10-11	0.3917091059674078
1107	12-13	0.29391576076071146
1107	14-15	0.19691867949119768
1107	16-17	0.28393493006040416
1107	18-19	0.43771834477620786
1107	20-21	0.2674505162834606
1107	22-23	0.04405116793887487
1107	24-25	0.10529972860211245
1107	26-27	0.2523659342182114
1107	28-29	0.15259576015650111
1107	30-31	0.26131812759670225
1107	32-33	0.20338507261015337
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2216	48-49	-0.5539526284028113
2216	50-51	-0.5425185721212529
2216	52-53	-0.5046350816982326
2216	54-55	-0.6388621341108802
2216	56-57	-0.7118278020550761
2216	58-59	-0.6457802680855664
2216	60-61	-0.6138995454314369
2216	62-63	-0.5520102694545699
2216	64-65	-0.43864064411016557
2216	66-67	-0.49808650185367753
2216	68-69	-0.6352293495159564
2216	70-71	-0.7763626299761839
2216	72-73	-0.6876997101632227
2216	74-75	-0.6765770173096755
2216	76-77	-0.720704610371218
2216	78-79	-0.6439227510233181
2216	80-81	-0.6437603416743798
2216	82-83	-0.7737630948695298
2216	84-85	-0.7385869907848637
2216	86-87	-0.748270465831677
2216	88-89	-0.7628198168610965
2216	90-91	-0.6928491676486495
2216	92-93	-0.7955927457097474
2216	94-95	-0.8292382165840451
2216	96-97	-0.862022710364279
2216	98-99	-0.8485022173328112
2216	100	-0.9120746023069017
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	17462.0
3	2959.0
4	2628.0
5	2583.0
6	3907.0
7	7734.0
8	8462.0
9	5726.0
10	3893.0
11	3487.0
12	3598.0
13	3213.0
14	3161.0
15	3055.0
16	3029.0
17	2771.0
18	2390.0
19	2124.0
20	1897.0
21	1954.0
22	2636.0
23	5594.0
24	9928.0
25	11283.0
26	9909.0
27	6629.0
28	6687.0
29	8475.0
30	10402.0
31	12943.0
32	17081.0
33	23002.0
34	33933.0
35	60162.0
36	112387.0
37	207121.0
38	209608.0
39	45798.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.50440741687046	19.60878472095485	12.001085885315911	21.88572197685878
2	17.231367380410127	23.286520594532025	35.15690177747275	24.325210247585098
3	19.55653234319828	29.429102508099454	26.63479214444323	24.379573004259036
4	12.407239129421104	19.830042182383938	34.020450893177006	33.74226779501795
5	13.188929138067317	42.382384887033766	30.051123407013975	14.377562567884947
6	29.030736032125372	38.05485507427628	17.3932470320185	15.521161861579847
7	27.297883878801382	36.85277421863364	19.040656858208624	16.80868504435635
8	26.13356892146797	35.18392815926304	18.23454436095013	20.447958558318867
9	26.234669674552514	20.076842083718986	17.701195952998752	35.98729228872975
10-11	24.85764919189088	29.80426515144193	23.604677805327203	21.73340785133999
12-13	25.13813395369251	28.976037830994194	23.68024441423756	22.205583801075736
14-15	23.326998002750553	29.263939954028583	22.26856381675336	25.140498226467507
16-17	22.757268350036075	31.598226839267653	23.0387522151717	22.60575259552457
18-19	22.769044465038007	30.500196144202945	24.643500804475497	22.08725858628355
20-21	25.224539365245963	28.361125593649845	23.890692809907534	22.523642231196657
22-23	24.562273234283165	28.482058290799067	24.03281685800927	22.922851616908492
24-25	23.703634633119588	29.34241603091195	23.933734439692472	23.020214896275988
26-27	23.326874729233257	29.102094839143234	24.66100856560998	22.91002186601353
28-29	23.231740909470798	29.59575095898495	24.6615623139808	22.510945817563453
30-31	23.347702293044907	29.816008332556326	23.904760063767707	22.93152931063106
32-33	23.001027971280482	29.551217759341007	24.17824840061769	23.269505868760817
34-35	23.54622291125517	29.17315477601597	24.83400957482915	22.446612737899713
36-37	23.43575817557846	29.190937922621142	24.186307742945157	23.186996158855237
38-39	23.278638385154537	29.111629199973393	24.44871574033174	23.16101667454033
40-41	22.602195783182186	29.454567366589373	24.81582901246604	23.1274078377624
42-43	22.849311081475317	29.612297869292505	24.185366208600367	23.35302484063181
44-45	22.53591824158933	29.23029172308647	24.54355381682123	23.690236218502967
46-47	23.078790249093448	28.933756798069965	24.742672409627666	23.244780543208922
48-49	22.67484228752159	29.178258017500998	24.30426315388577	23.842636541091647
50-51	21.952858316303473	29.289918796701848	24.601046519229293	24.15617636776539
52-53	22.636397640234705	28.99063074493593	23.856640506424096	24.51633110840527
54-55	22.26179100897375	29.063523344984176	24.39481072256647	24.27987492347561
56-57	22.839903367912463	28.46605087395197	24.164558760835583	24.529486997299983
58-59	23.133498630669592	28.005052141419036	24.296605265641006	24.564843962270373
60-61	23.222934968892726	28.03138864335919	24.53572149258058	24.209954895167503
62-63	22.27127727301665	27.852298254708828	24.49182832664866	25.38459614562586
64-65	22.811254796077872	28.510672161432428	24.210657950831322	24.467415091658378
66-67	23.042817905669644	28.245845990141333	23.96735225639813	24.743983847790894
68-69	23.077740741414434	28.154558352735737	24.289235302677533	24.478465603172296
70-71	23.260353411764438	28.023573668566907	24.917592771014483	23.79848014865417
72-73	23.026552505614546	27.654838458473378	24.863053916143578	24.455555119768494
74-75	22.91580100372375	28.342377912393303	24.670697553330996	24.071123530551958
76-77	23.33108993015134	27.549578452415602	24.947704452852154	24.171627164580908
78-79	23.32074450669383	27.685546875	25.102942647700814	23.890765970605354
80-81	23.237863426457515	27.279067863858465	25.865493273185734	23.61757543649828
82-83	23.547049478049072	27.260020519933043	25.4548348315916	23.738095170426284
84-85	23.1024892012967	27.47761958437711	25.251543423036892	24.168347791289303
86-87	23.84765404070691	27.02313871919939	25.12603317646805	24.00317406362565
88-89	23.150359633229012	27.16649279818445	25.083559819908857	24.599587748677685
90-91	23.500569427546566	26.728488594575023	25.253811389351732	24.51713058852668
92-93	24.046451663089883	27.07239897684166	25.12829517843497	23.752854181633484
94-95	23.338781607328517	26.92091691134335	25.673265979093724	24.0670355022344
96-97	23.28128958539809	26.872691358137658	25.434334919561042	24.411684136903215
98-99	22.78858306216867	27.425927726277145	25.010379554279638	24.77510965727455
100	22.872410389438084	27.51034814842773	24.752061909320595	24.865179552813586
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5599.0
1	4075.0
2	2494.0
3	2189.0
4	1598.0
5	1050.0
6	800.5
7	746.5
8	765.0
9	781.5
10	677.0
11	553.5
12	515.5
13	496.0
14	463.5
15	457.5
16	501.5
17	572.0
18	648.5
19	784.0
20	945.0
21	1147.0
22	1408.5
23	1730.5
24	2088.0
25	2524.5
26	3141.5
27	3656.0
28	4244.5
29	5034.5
30	6113.0
31	7230.0
32	8047.0
33	9163.5
34	10502.5
35	12371.0
36	14669.5
37	16673.5
38	18600.5
39	20198.0
40	22058.5
41	23294.0
42	25056.5
43	27572.0
44	28809.5
45	29901.5
46	31973.5
47	35665.0
48	41405.0
49	42610.5
50	39448.0
51	36073.5
52	31754.0
53	28710.0
54	26916.0
55	26520.5
56	26657.0
57	26189.5
58	24836.0
59	23113.5
60	21259.0
61	17765.5
62	14281.5
63	11564.0
64	8404.0
65	6949.5
66	6270.5
67	4730.0
68	3659.5
69	2825.5
70	2002.5
71	1609.0
72	1385.5
73	1281.0
74	1196.5
75	1011.0
76	688.5
77	435.5
78	336.0
79	344.5
80	271.5
81	150.5
82	94.0
83	46.0
84	16.5
85	9.5
86	3.5
87	3.0
88	5.0
89	4.5
90	2.5
91	3.5
92	4.5
93	3.5
94	1.5
95	1.5
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33479262751392885
2	0.11845633883514901
3	0.06559434501716026
4	0.04228959505439101
5	0.045927409682725726
6	0.010799762177868672
7	0.00773035608521126
8	0.016824892656048036
9	0.020803752405789126
10-11	0.024953134716233408
12-13	0.018018550580970363
14-15	0.021542683502169615
16-17	0.02302054569493059
18-19	0.022054251184279183
20-21	0.02699940544467168
22-23	0.018416436555944472
24-25	0.02819306336959401
26-27	0.022963704841362863
28-29	0.02313422740206605
30-31	0.02353211337704016
32-33	0.02807938166245855
34-35	0.017677505459563987
36-37	0.010629239617165482
38-39	0.015290189609719331
40-41	0.011311329859978242
42-43	0.009321899985107697
44-45	0.009321899985107697
46-47	0.009037695717269048
48-49	0.004774631699689308
50-51	0.007559833524508071
52-53	0.00795771949948218
54-55	0.003126246946225142
56-57	0.002728360971251033
58-59	0.002500997556980114
60-61	0.003580973774766981
62-63	0.0028420426783864927
64-65	0.002728360971251033
66-67	0.001932589021302815
68-69	0.0037514963354701703
70-71	0.0026715201176833034
72-73	0.0016483847534641658
74-75	0.002785201824818763
76-77	0.0037514963354701703
78-79	0.0037514963354701703
80-81	0.00397885974974109
82-83	0.0018757481677350852
84-85	0.0033536103604960613
86-87	0.0022167932891414646
88-89	0.616723261209869
90-91	0.6281482727769827
92-93	0.6244536172950802
94-95	0.6100160404888768
96-97	0.6084813374425481
98-99	0.6063782258605421
100	0.6068329526890839
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	879649.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.351311774614096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.44603663995031	20.970348964796838
2	9.582497440397843	5.625177399051173
3	4.418745411389128	3.8908792266698313
4	2.7753395068120175	3.2583942057937296
5	1.9004904962703386	2.7890944540360887
6	1.3858876660400474	2.440657258232022
7	1.0666859302993659	2.1916041914067677
8	0.8551774129538385	2.008046309617282
9	0.7090389297567448	1.873010041886603
>10	5.1580422178249155	29.456738693074662
>50	0.4601170449241777	9.238201252823865
>100	0.22518852392015717	11.629542065072513
>500	0.014415182327068862	2.7867721425561403
>1k	0.0019479976117660626	0.9627342908567972
>5k	3.8959952235321246E-4	0.8787995041257258
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7685	0.8736439193360078	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	3239	0.36821504941175404	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	1758	0.19985244114413817	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	1292	0.14687676561901394	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	1109	0.1260730132132248	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	1021	0.11606902298530437	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	973	0.11061230104280229	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	967	0.10993021079998955	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	5.6840853567729855E-5	0.0
14-15	0.0	0.0	0.0	2.842042678386493E-4	0.0
16-17	0.0	0.0	0.0	5.684085356772986E-4	0.0
18-19	0.0	0.0	0.0	9.094536570836777E-4	0.0
20-21	0.0	0.0	0.0	0.0015347030463287062	0.0
22-23	0.0	0.0	0.0	0.0034672920676315213	0.0
24-25	0.0	0.0	0.0	0.009094536570836777	0.0
26-27	0.0	0.0	0.0	0.01853011826307993	0.0
28-29	0.0	0.0	0.0	0.04456322919710021	0.0
30-31	0.0	0.0	0.0	0.11021441506782818	0.0
32-33	0.0	0.0	0.0	0.21070904417557457	0.0
34-35	0.0	0.0	0.0	0.34991229456294504	0.0
36-37	0.0	0.0	0.0	0.5627812911740933	0.0
38-39	0.0	0.0	0.0	0.866481971786474	0.0
40-41	0.0	0.0	0.0	1.2161100620815803	0.0
42-43	0.0	0.0	0.0	1.51156881892664	0.0
44-45	0.0	0.0	0.0	1.8326628007307462	0.0
46-47	0.0	0.0	0.0	2.2050840733065122	0.0
48-49	0.0	0.0	0.0	2.598024894020228	0.0
50-51	0.0	0.0	0.0	3.0142704646967147	0.0
52-53	0.0	0.0	0.0	3.387999076904538	0.0
54-55	0.0	0.0	0.0	3.780030443961171	0.0
56-57	0.0	0.0	0.0	4.2331088877495455	0.0
58-59	0.0	0.0	0.0	4.702330133951156	0.0
60-61	0.0	0.0	0.0	5.2044053935149135	0.0
62-63	0.0	0.0	0.0	5.706935379907213	0.0
64-65	0.0	0.0	0.0	6.206907527888965	0.0
66-67	0.0	0.0	0.0	6.730866516076299	0.0
68-69	0.0	0.0	0.0	7.345429824850594	0.0
70-71	0.0	0.0	0.0	8.022461231695823	0.0
72-73	0.0	0.0	0.0	8.683861403809928	0.0
74-75	0.0	0.0	0.0	9.351968796645025	0.0
76-77	0.0	0.0	0.0	10.035139015675572	0.0
78-79	0.0	0.0	0.0	10.802376857132789	0.0
80-81	0.0	0.0	0.0	11.61906624119393	0.0
82-83	0.0	0.0	0.0	12.4728727026348	0.0
84-85	0.0	0.0	0.0	13.307353273862642	0.0
86-87	0.0	0.0	0.0	14.125349997555844	0.0
88	0.0	0.0	0.0	14.77668933858846	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATATCG	65	1.4157566E-4	36.142822	5
GAGAACA	775	0.0	34.02448	1
CATGGGT	825	0.0	31.893301	4
GTACATG	7500	0.0	29.382568	1
GGCGTCG	65	0.006176279	28.901104	9
TACATGG	7415	0.0	28.530792	2
GTACAGG	380	0.0	28.500298	1
ACGTGCG	85	6.8451476E-4	27.626057	9
TACATGA	290	0.0	27.558996	2
GTACATA	480	0.0	27.467678	1
CGTATCA	90	9.28238E-4	26.222475	94
GTACAAA	530	0.0	25.76483	1
ACATGGG	7865	0.0	25.337051	3
ATATATC	225	1.1641532E-10	25.06615	3
CCATATG	150	1.1697321E-6	25.047623	8
AGTACGG	255	2.1827873E-11	23.953476	5
GAACAAA	1305	0.0	23.81434	1
TATACGG	100	0.0017556526	23.506205	2
ACTAGGG	120	1.8378228E-4	23.499517	3
GCAGCGA	50	0.0017671366	23.48148	38-39
>>END_MODULE