FastQCFastQC Report
Wed 25 May 2016
SRR1033173_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033173_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879649
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100241.1395454323258483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87050.9895992606141768No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49630.5642023125132866No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31430.3573016055267499No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT21740.2471440313124894No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19320.21963305818570814No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18710.21269847405044512No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18670.2122437472219033No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16930.1924631301803333No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16920.19234944847319782No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13280.15096930707589049No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC10820.1230036071205674No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT10510.11947947419936816No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9910.11265857177124058No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT9790.11129439128561507TruSeq Adapter, Index 3 (95% over 21bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9270.10538294251457114No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA200.002160808870.516462
CGGTATA301.3694003E-462.68132
GCGTATC250.0052260956.4260031
TATAACG352.9337464E-453.7268262
CTACCGT354.6310306E-640.2928326-27
GCGTAGA500.001702710537.5959594
ACGTGCG959.4552706E-734.627859
GGTATCA84650.033.051261
CGTGACT300.004163768631.32996270-71
GTATCAA126100.029.533041
TACCGTA404.7769613E-429.38018628-29
GCGTTCG404.7849186E-429.37183878-79
AACCCGC753.6938218E-828.2049828-29
AACCGTG1354.634676E-727.8504378
TAAGGAC1555.0691597E-827.2966924
CGGGAGT3800.027.216884
GTCGGGA3650.027.047412
TAATCGA350.00884191226.85425292-93
GTGGGTA4100.026.3630149
GTACATG80950.025.9650231
Produced by FastQC (version 0.11.5)