##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033173_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	879649
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63866951477237	34.0	31.0	34.0	31.0	34.0
2	32.68041457444958	34.0	31.0	34.0	31.0	34.0
3	32.76291111568364	34.0	31.0	34.0	31.0	34.0
4	36.25615671705419	37.0	35.0	37.0	35.0	37.0
5	36.22820465890372	37.0	37.0	37.0	35.0	37.0
6	36.3361033775972	37.0	37.0	37.0	35.0	37.0
7	36.317331117297925	37.0	37.0	37.0	35.0	37.0
8	36.31428331072962	37.0	37.0	37.0	35.0	37.0
9	38.10964032244679	39.0	39.0	39.0	37.0	39.0
10-11	38.09076347497695	39.0	39.0	39.0	36.0	39.0
12-13	38.04464905888599	39.0	39.0	39.0	35.0	39.0
14-15	39.44262768445141	41.0	39.0	41.0	36.0	41.0
16-17	39.4512339580901	41.0	39.0	41.0	36.5	41.0
18-19	39.54628778069434	41.0	39.5	41.0	37.0	41.0
20-21	39.54472465722123	41.0	40.0	41.0	37.0	41.0
22-23	39.42789567202372	41.0	39.0	41.0	36.0	41.0
24-25	39.32735215978191	41.0	39.0	41.0	36.0	41.0
26-27	39.151169955288985	41.0	39.0	41.0	35.0	41.0
28-29	38.9990854306661	41.0	39.0	41.0	35.0	41.0
30-31	38.708703698861704	40.0	38.0	41.0	35.0	41.0
32-33	38.50159495435111	40.0	38.0	41.0	35.0	41.0
34-35	38.305085892213825	40.0	37.5	41.0	35.0	41.0
36-37	38.099782981621075	40.0	37.0	41.0	35.0	41.0
38-39	37.89308974375007	40.0	37.0	41.0	34.0	41.0
40-41	37.65350327232794	40.0	36.5	41.0	33.5	41.0
42-43	37.337199837662524	40.0	36.0	41.0	33.0	41.0
44-45	36.894909219472765	40.0	35.5	41.0	32.5	41.0
46-47	36.21103019499823	40.0	35.0	41.0	30.5	41.0
48-49	35.609147512246366	40.0	35.0	41.0	27.0	41.0
50-51	35.2643236108948	40.0	35.0	41.0	24.0	41.0
52-53	35.060695800256696	39.0	35.0	41.0	23.5	41.0
54-55	34.83579871062208	39.0	35.0	41.0	23.0	41.0
56-57	34.58648676915452	39.0	35.0	41.0	22.5	41.0
58-59	34.303207302003415	38.0	35.0	41.0	22.0	41.0
60-61	33.99421303269827	37.0	35.0	40.5	21.5	41.0
62-63	33.68217550409311	37.0	35.0	40.0	20.0	41.0
64-65	33.35380361939819	36.0	35.0	39.5	18.5	41.0
66-67	32.976996506561136	35.5	34.0	39.0	16.0	41.0
68-69	32.58077483177949	35.0	34.0	38.5	10.0	41.0
70-71	32.17355899910078	35.0	34.0	37.0	4.5	40.0
72-73	31.822728724752714	35.0	34.0	37.0	2.0	39.0
74-75	31.54455413466053	35.0	33.5	36.0	2.0	39.0
76-77	31.2730287876187	35.0	33.0	36.0	2.0	38.5
78-79	31.032939843051032	35.0	33.0	35.5	2.0	37.0
80-81	30.793234574244956	35.0	33.0	35.0	2.0	37.0
82-83	30.587078482440155	35.0	33.0	35.0	2.0	36.0
84-85	30.422950517763333	35.0	33.0	35.0	2.0	36.0
86-87	30.30645689360188	35.0	33.0	35.0	2.0	36.0
88-89	30.165281834004247	35.0	33.0	35.0	2.0	35.5
90-91	30.026097341098552	35.0	33.0	35.0	2.0	35.0
92-93	29.91263674488347	35.0	33.0	35.0	2.0	35.0
94-95	29.775327431736976	35.0	32.0	35.0	2.0	35.0
96-97	29.687835716291385	35.0	32.0	35.0	2.0	35.0
98-99	29.60436037555889	35.0	32.0	35.0	2.0	35.0
100	29.50719548365314	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.029387399821651172
1101	2	0.098342475666918
1101	3	0.15297929316966474
1101	4	0.15919891223777682
1101	5	0.06278916747289998
1101	6	0.07538297822021178
1101	7	0.1366369976261126
1101	8	0.07912060696873624
1101	9	0.19364793694468574
1101	10-11	0.1267651932182403
1101	12-13	0.1734769232957447
1101	14-15	0.1470244744036151
1101	16-17	0.1552741490805758
1101	18-19	0.1607184714002372
1101	20-21	0.20644971322550987
1101	22-23	0.19771302207484354
1101	24-25	0.11515003626595188
1101	26-27	0.09808507560415336
1101	28-29	0.14926194493381928
1101	30-31	0.14684104163041667
1101	32-33	0.09091661150154806
1101	34-35	0.10377620489143169
1101	36-37	0.23007553857566876
1101	38-39	0.24405765113188949
1101	40-41	0.14220408420026587
1101	42-43	0.1311274869825354
1101	44-45	0.019538769468880446
1101	46-47	0.1338656502238038
1101	48-49	0.28380475181655385
1101	50-51	0.3935605770356716
1101	52-53	0.40782541340519174
1101	54-55	0.4410809815834682
1101	56-57	0.4575449070513642
1101	58-59	0.424664936467785
1101	60-61	0.4063620572656177
1101	62-63	0.3298896704289902
1101	64-65	0.37924085276262076
1101	66-67	0.3475554899500395
1101	68-69	0.41693103431640566
1101	70-71	0.5106673553933518
1101	72-73	0.6826605640149168
1101	74-75	0.602583964451906
1101	76-77	0.5858324125333496
1101	78-79	0.6579115871857724
1101	80-81	0.5639021029913813
1101	82-83	0.5312174134667522
1101	84-85	0.6371773441270214
1101	86-87	0.5279281744386957
1101	88-89	0.5484182118504748
1101	90-91	0.504423386252423
1101	92-93	0.4513465859184791
1101	94-95	0.4596810139308296
1101	96-97	0.5411069002571445
1101	98-99	0.5328078310380526
1101	100	0.5223079734025795
1102	1	0.03712477982468698
1102	2	0.05449912060118578
1102	3	0.15897639347281256
1102	4	0.043318320895792795
1102	5	0.05446403359947993
1102	6	0.13202285487581378
1102	7	0.11508774416011391
1102	8	0.16541417522042678
1102	9	0.161935951020169
1102	10-11	0.11630781661627765
1102	12-13	0.13351643396423185
1102	14-15	0.1544311921368262
1102	16-17	0.13265321854149903
1102	18-19	0.13682011098569546
1102	20-21	0.16021548760703297
1102	22-23	0.19506045847922593
1102	24-25	0.10381792288964675
1102	26-27	0.23750041579744163
1102	28-29	0.2234287418722758
1102	30-31	0.13356443016832742
1102	32-33	0.16949617384962323
1102	34-35	0.2602590657213071
1102	36-37	0.226939819889175
1102	38-39	0.10184185054275474
1102	40-41	0.18854137779610625
1102	42-43	0.11829609853043621
1102	44-45	0.06856847450288939
1102	46-47	0.19039364639840528
1102	48-49	-0.0038610381975772157
1102	50-51	-0.06100138789209808
1102	52-53	-0.1715480405751535
1102	54-55	-0.21273054118650236
1102	56-57	-0.07531845163132544
1102	58-59	-0.2427312522393379
1102	60-61	-0.14923296298015032
1102	62-63	-0.15377003206089057
1102	64-65	-0.22945218398015754
1102	66-67	-0.1594067710778475
1102	68-69	-0.11528019909692944
1102	70-71	-0.14828760428369492
1102	72-73	-0.026765902922093687
1102	74-75	0.06092879235519888
1102	76-77	-0.12501593270835798
1102	78-79	-0.03162046120790052
1102	80-81	0.12097762384866328
1102	82-83	0.021897232986678006
1102	84-85	-0.04449510047681571
1102	86-87	0.015117476212537895
1102	88-89	0.028413445435461426
1102	90-91	0.05094037829204723
1102	92-93	-0.011935926089513771
1102	94-95	-0.05043075926751328
1102	96-97	-0.14141729198111008
1102	98-99	-0.005306247204718062
1102	100	-0.0481062295945911
1103	1	0.2103862303455699
1103	2	0.217118142432021
1103	3	0.2133755317042869
1103	4	0.11651559559703628
1103	5	0.0782112972854705
1103	6	0.13052713829863194
1103	7	0.1454493170645108
1103	8	0.21255429881230015
1103	9	0.2044496685095183
1103	10-11	0.18535429361428868
1103	12-13	0.17757523404587516
1103	14-15	0.3294502938374464
1103	16-17	0.3291966396358177
1103	18-19	0.25778526815751235
1103	20-21	0.31186420199073694
1103	22-23	0.37967535566897226
1103	24-25	0.29414233128952816
1103	26-27	0.35485927504461756
1103	28-29	0.29368763346524673
1103	30-31	0.2701819297575625
1103	32-33	0.2730517058273847
1103	34-35	0.29270985259348237
1103	36-37	0.382022787950838
1103	38-39	0.4576367207506564
1103	40-41	0.43413509254453686
1103	42-43	0.2682884725863204
1103	44-45	0.2882844504505897
1103	46-47	0.32962791319151563
1103	48-49	0.4917649118219103
1103	50-51	0.45372773636216834
1103	52-53	0.3179734701133299
1103	54-55	0.3162484148917457
1103	56-57	0.41898235244527626
1103	58-59	0.4295174581656198
1103	60-61	0.24513481677963256
1103	62-63	0.29197782254366444
1103	64-65	0.24558150648314125
1103	66-67	0.2693934358173351
1103	68-69	0.38942136123755233
1103	70-71	0.4535706946223641
1103	72-73	0.3593422793843857
1103	74-75	0.32302475546773124
1103	76-77	0.27449512270020193
1103	78-79	0.22485256542248777
1103	80-81	0.2959186240925469
1103	82-83	0.328204146660255
1103	84-85	0.3795888099599729
1103	86-87	0.3133582040591705
1103	88-89	0.4125860378659567
1103	90-91	0.3798720314125319
1103	92-93	0.3280327154387983
1103	94-95	0.3167131367720799
1103	96-97	0.363836504597014
1103	98-99	0.32674651696056145
1103	100	0.2951725137677066
1104	1	0.09833172063273565
1104	2	0.10114408298233002
1104	3	0.15937497085814556
1104	4	-0.014868986232684733
1104	5	0.03198327084072616
1104	6	-0.013691047845640014
1104	7	0.05995073051008859
1104	8	0.034724999983275495
1104	9	0.024915170806721676
1104	10-11	0.05175075801755469
1104	12-13	0.10927415880751568
1104	14-15	0.15583583873630147
1104	16-17	0.2420473809157926
1104	18-19	0.12576761000173065
1104	20-21	0.10589381070141712
1104	22-23	0.09616727721849117
1104	24-25	2.3700098514467527E-4
1104	26-27	0.15312001468171133
1104	28-29	0.03967815006685527
1104	30-31	-0.008749799971248251
1104	32-33	0.037889343702062206
1104	34-35	0.15941506563004282
1104	36-37	0.1723789252505128
1104	38-39	0.14617520669267492
1104	40-41	0.17319115733076274
1104	42-43	0.20273639320676295
1104	44-45	0.20643632078749619
1104	46-47	0.2876565106796036
1104	48-49	0.1624444845591242
1104	50-51	0.12523229111263845
1104	52-53	0.02451780811659887
1104	54-55	0.11080948197233909
1104	56-57	0.19826071624746788
1104	58-59	0.003270496082215857
1104	60-61	-0.0623901522038679
1104	62-63	-0.06646411571793465
1104	64-65	0.02756106675710157
1104	66-67	0.11822875949353318
1104	68-69	-0.03877794025407866
1104	70-71	0.04080599223905779
1104	72-73	-0.0284792865660215
1104	74-75	0.003327229988887126
1104	76-77	0.012229091848002582
1104	78-79	-0.04166831901488166
1104	80-81	-0.012788583063628067
1104	82-83	0.055415523339252815
1104	84-85	0.10233253726411462
1104	86-87	0.018557077834795876
1104	88-89	0.08285205738793877
1104	90-91	0.1400848130394401
1104	92-93	0.1444586384272597
1104	94-95	0.16717965878954288
1104	96-97	0.15857850120928063
1104	98-99	0.17484431379829957
1104	100	0.184692197201219
1105	1	0.07443685642912357
1105	2	0.036686304871430764
1105	3	-0.007854948782942017
1105	4	0.015745430188161436
1105	5	0.044541023789271605
1105	6	-0.030152311209789673
1105	7	-0.0048148848699938185
1105	8	5.108951472863055E-4
1105	9	0.024616702497894494
1105	10-11	0.039141810392727905
1105	12-13	0.03149137681519676
1105	14-15	0.11152467147091016
1105	16-17	0.1180719436530282
1105	18-19	0.11125709802820438
1105	20-21	0.1486025511383815
1105	22-23	0.15604731437310448
1105	24-25	0.08938600493080884
1105	26-27	0.04435474996842714
1105	28-29	0.182410245983597
1105	30-31	0.26934102005370164
1105	32-33	0.22348644093045777
1105	34-35	0.1682075003283856
1105	36-37	0.12570611076127847
1105	38-39	0.16067768812875727
1105	40-41	0.2584209243916362
1105	42-43	0.12617023763918667
1105	44-45	0.3081042634173272
1105	46-47	0.34743984677901096
1105	48-49	0.46192229886097635
1105	50-51	0.6284281104148448
1105	52-53	0.6428263627804682
1105	54-55	0.6018012265798376
1105	56-57	0.6128040865034308
1105	58-59	0.5124490657899585
1105	60-61	0.5414714655746664
1105	62-63	0.5109452462826383
1105	64-65	0.5057835875963548
1105	66-67	0.3966083442612103
1105	68-69	0.5405105675114541
1105	70-71	0.43876764106900623
1105	72-73	0.27369846135005815
1105	74-75	0.28825839489865146
1105	76-77	0.39460413668516736
1105	78-79	0.32004582597379283
1105	80-81	0.3772890856178144
1105	82-83	0.3945257130205455
1105	84-85	0.4728160291559469
1105	86-87	0.41051958057856197
1105	88-89	0.4178061093294545
1105	90-91	0.4142794839747843
1105	92-93	0.43452466282938573
1105	94-95	0.4898413001243256
1105	96-97	0.6262432075325215
1105	98-99	0.49599688330259895
1105	100	0.5531727392089572
1106	1	0.07298197216591262
1106	2	0.12074759934473889
1106	3	0.12904430421869506
1106	4	0.0882685457829524
1106	5	0.13816930335126187
1106	6	0.08410841949586256
1106	7	0.044161028574357886
1106	8	0.07321773970511458
1106	9	0.10691855859639077
1106	10-11	0.06502704144902083
1106	12-13	0.05716329543135856
1106	14-15	0.21879311381053412
1106	16-17	0.1525413655482879
1106	18-19	0.14239915726241748
1106	20-21	0.15212578755832595
1106	22-23	0.25646546903677603
1106	24-25	0.31674405717959786
1106	26-27	0.16266156612611127
1106	28-29	0.1548783051704774
1106	30-31	0.2604136608803955
1106	32-33	0.2521453565185112
1106	34-35	0.1753776009620367
1106	36-37	0.12996395162833352
1106	38-39	-0.03282534433334661
1106	40-41	-0.01999384965910167
1106	42-43	-0.1272038357751697
1106	44-45	-0.1587357728053007
1106	46-47	-0.32917631414514403
1106	48-49	-0.4218133905212369
1106	50-51	-0.34293383813357536
1106	52-53	-0.33493994145227646
1106	54-55	-0.29260662431445894
1106	56-57	-0.27078679954350804
1106	58-59	-0.25846240425349976
1106	60-61	-0.22476425806209477
1106	62-63	-0.3006444389865379
1106	64-65	-0.2508244269724287
1106	66-67	-0.3483378636352725
1106	68-69	-0.37606900955069733
1106	70-71	-0.3568862035996787
1106	72-73	-0.21755136893420968
1106	74-75	-0.2749287981338462
1106	76-77	-0.30385895496880266
1106	78-79	-0.42227679461156953
1106	80-81	-0.3048865891657293
1106	82-83	-0.1975296597748617
1106	84-85	-0.1905071855901994
1106	86-87	-0.1646641795249728
1106	88-89	-0.13299899721466346
1106	90-91	-0.1791837509254961
1106	92-93	-0.16192650325785962
1106	94-95	-0.23608210918768435
1106	96-97	-0.291368054367819
1106	98-99	-0.3317463146775026
1106	100	-0.28114546440757593
1107	1	0.032131414366205036
1107	2	0.06344993187757808
1107	3	0.10091833541475381
1107	4	0.09062143796635524
1107	5	5.089156908724135E-4
1107	6	-0.044496687922801925
1107	7	0.026522434664570937
1107	8	0.039875846193730524
1107	9	0.049928594826766926
1107	10-11	0.0696506943777635
1107	12-13	0.11815514244015901
1107	14-15	0.12943526597810973
1107	16-17	0.1252708361591104
1107	18-19	0.05759844746170728
1107	20-21	0.07438838143108484
1107	22-23	0.09045585387462296
1107	24-25	0.13944657479736833
1107	26-27	0.12361327284053658
1107	28-29	0.1424116782970728
1107	30-31	0.13902628737437084
1107	32-33	0.20826750331863053
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2216	38-39	-0.44652822726305175
2216	40-41	-0.5591720664662532
2216	42-43	-0.3892463898232421
2216	44-45	-0.37219819809690335
2216	46-47	-0.21132690943333898
2216	48-49	-0.06389201321501758
2216	50-51	-0.03682568343549519
2216	52-53	0.18104161614226655
2216	54-55	0.23841680292056822
2216	56-57	0.23826421627106953
2216	58-59	0.16389896661123515
2216	60-61	0.17119709669892558
2216	62-63	0.18748914127031213
2216	64-65	0.09309999173861883
2216	66-67	0.08171348523325861
2216	68-69	0.24928874895956454
2216	70-71	0.15380857989694618
2216	72-73	0.10543594207597096
2216	74-75	0.04705294611731503
2216	76-77	0.06945152817903022
2216	78-79	0.06530459316205395
2216	80-81	0.0077170327045728015
2216	82-83	-0.03417721548820296
2216	84-85	-0.044206371986959425
2216	86-87	-0.056178257459595926
2216	88-89	-0.13320852841376762
2216	90-91	-0.15173653631796924
2216	92-93	-0.0817913419096854
2216	94-95	-0.13846481746535488
2216	96-97	-0.28864094633755855
2216	98-99	-0.29911983652181817
2216	100	-0.2959416610045693
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6.0
3	9.0
4	33.0
5	69.0
6	172.0
7	322.0
8	488.0
9	672.0
10	1111.0
11	1918.0
12	3035.0
13	4586.0
14	4918.0
15	6339.0
16	9286.0
17	14887.0
18	18181.0
19	8373.0
20	3975.0
21	3337.0
22	3150.0
23	3426.0
24	3757.0
25	4444.0
26	5410.0
27	6335.0
28	6351.0
29	7755.0
30	9604.0
31	11852.0
32	15340.0
33	20032.0
34	29976.0
35	55338.0
36	108191.0
37	214759.0
38	240476.0
39	51673.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.77504827204841	18.141187634618426	12.906187810980654	21.17757628235251
2	18.01923063805793	20.964987870010162	37.01556776764988	24.000213724282034
3	19.63817386252926	27.663761341171305	29.626476014865023	23.071588781434414
4	12.489200225544764	17.98810433264215	35.812961548255664	33.70973389355743
5	12.537073765420867	38.9175597729175	32.558840387204775	15.986526074456858
6	29.009899414311178	36.68478755138419	17.40287023900469	16.902442795299937
7	26.655616339931697	34.69643433695766	21.033933143249524	17.61401617986112
8	27.94135368091255	30.424625360656442	19.506824336153407	22.127196622277598
9	28.299160573320236	14.069946160282296	19.89955072938266	37.731342537014804
10-11	26.24164793283673	25.345493507342432	25.306330092451805	23.10652846736903
12-13	27.93727952853922	24.594412089367463	26.380692753586942	21.08761562850637
14-15	24.913189757045	24.26128163692657	26.986972637365948	23.838555968662483
16-17	21.74634428050279	27.416389946444546	27.45987319942386	23.3773925736288
18-19	21.359440731155683	24.174087717991902	32.64337971360151	21.823091837250903
20-21	23.337945021252796	22.27593051319333	32.88340008344237	21.502724382111502
22-23	22.77477878948239	22.65046777207032	33.15807393521389	21.416679503233393
24-25	22.083744956783804	23.55109492785174	33.074005822790284	21.29115429257418
26-27	21.827689894103806	23.27226633774236	33.70693526292155	21.19310850523228
28-29	21.69704707646586	23.5156082126587	33.68354273513505	21.103801975740392
30-31	21.899951440466808	23.677207610768615	33.126926886628844	21.295914062135733
32-33	21.672280240523257	23.388475988610622	33.18884350523529	21.75040026563083
34-35	22.383797952001093	22.82410066011292	33.62351389307421	21.168587494811774
36-37	22.157185263604365	22.970681061092353	32.894101700620126	21.978031974683155
38-39	22.12566385064931	22.80269059570735	32.90953778767809	22.16210776596525
40-41	21.716335211362104	23.219269951141687	32.91553874066885	22.148856096827366
42-43	22.32228441997469	23.297720287496887	31.875040508134035	22.50495478439439
44-45	21.898599528002332	23.261718783312592	31.754777256557627	23.084904432127452
46-47	23.026331123960432	23.006149443355138	31.000710054058477	22.966809378625953
48-49	22.939663670068775	23.74080925197231	29.579513684792165	23.740013393166755
50-51	22.303657123756924	24.357158205534287	29.372500912376605	23.966683758332184
52-53	22.756526860058482	24.295585700708052	28.55555277650194	24.39233466273153
54-55	22.416720525549007	24.574827466836286	28.804185019897943	24.204266987716764
56-57	22.90433185088174	24.05323183542059	28.452327358052987	24.590108955644684
58-59	22.98319597608341	23.721793164366577	28.7391837915603	24.555827067989707
60-61	23.10022793285928	23.998942755316065	28.8964298097528	24.00439950207186
62-63	21.88834005013443	23.97033997055597	28.940867518914093	25.200452460395507
64-65	22.369128592590233	24.540740749161856	28.613963027342393	24.47616763090551
66-67	22.57014055109763	24.36774174112383	28.194003372953507	24.868114334825034
68-69	22.646733489533773	24.27421931042166	28.320026738241484	24.75902046180308
70-71	22.927234364733085	24.088155917266718	28.758784783878973	24.225824934121228
72-73	22.711176339519167	23.925247031753756	28.293264093201344	25.070312535525733
74-75	22.532012950693687	24.51889628076813	28.017357021704235	24.93173374683395
76-77	22.871543138684842	23.987106169473954	28.117292704845305	25.024057986995896
78-79	22.67895794681509	24.28960220451817	27.997335297755466	25.034104550911273
80-81	22.466702438713842	24.097646320556954	28.609755764832133	24.825895475897067
82-83	22.608588890101494	24.088385354141657	28.09873949579832	25.20428625995853
84-85	22.25570158012338	24.2737281396561	27.745179455610863	25.725390824609658
86-87	22.95436524289559	23.926004450646424	27.64010583784982	25.479524468608165
88-89	22.163739266309214	24.07370671353387	27.624727376901113	26.137826643255803
90-91	22.435255425743676	23.686720498744386	27.763175994061267	26.11484808145067
92-93	22.87406681528655	24.000197806170416	27.656940438743185	25.468794939799857
94-95	22.45298977205681	23.604130738510474	28.249449496333195	25.69342999309952
96-97	22.493630982357736	23.676943871930735	27.88134812862858	25.948077017082948
98-99	22.193681798080824	23.8799225600211	27.499434433507002	26.426961208391077
100	22.293892222920732	23.78323626810239	27.140029716398246	26.782841792578633
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	710.0
1	488.5
2	731.5
3	1266.5
4	828.0
5	497.5
6	1077.5
7	2166.5
8	3530.0
9	3929.5
10	2626.0
11	1330.0
12	1038.5
13	1037.0
14	1106.0
15	1185.0
16	1258.5
17	1310.5
18	1416.5
19	1502.5
20	1641.0
21	1920.5
22	2168.5
23	2473.5
24	2814.5
25	3094.5
26	3486.5
27	3987.0
28	4541.5
29	5258.5
30	6251.5
31	7248.5
32	8087.5
33	9210.0
34	10579.0
35	12273.0
36	14201.0
37	16102.0
38	17958.0
39	19426.5
40	21259.5
41	22484.0
42	24115.5
43	26821.0
44	28546.5
45	29729.0
46	31720.5
47	35659.5
48	41564.0
49	42806.5
50	40471.5
51	37051.0
52	31607.0
53	27878.5
54	25925.0
55	25606.0
56	26103.5
57	25815.0
58	24391.0
59	22746.0
60	20852.5
61	17697.5
62	14366.5
63	11442.5
64	8287.5
65	6840.5
66	6074.5
67	4520.5
68	3462.0
69	2678.5
70	1853.0
71	1447.5
72	1191.0
73	1116.5
74	1098.0
75	937.5
76	635.0
77	376.0
78	267.0
79	283.5
80	243.5
81	142.5
82	84.0
83	30.0
84	4.5
85	3.0
86	2.0
87	2.0
88	1.0
89	0.5
90	0.0
91	0.0
92	1.5
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08821700473711673
2	0.001250498778490057
3	0.0
4	1.1368170713545971E-4
5	0.03649182799048257
6	1.1368170713545971E-4
7	0.004206223164012009
8	3.4104512140637914E-4
9	1.1368170713545971E-4
10-11	1.7052256070318957E-4
12-13	0.0
14-15	5.6840853567729855E-5
16-17	0.0
18-19	5.6840853567729855E-5
20-21	0.0
22-23	5.6840853567729855E-5
24-25	2.2736341427091942E-4
26-27	0.001591543899896436
28-29	0.009094536570836777
30-31	0.03592341945480527
32-33	0.02648783776256211
34-35	0.02887515361240677
36-37	0.026658360323265306
38-39	0.02449840788769157
40-41	0.030637220073006392
42-43	0.022281614598550104
44-45	0.022224773744982374
46-47	0.01574491643826117
48-49	0.010913443885004133
50-51	0.008867173156565858
52-53	0.006025130478179364
54-55	0.00403570060330882
56-57	0.0028420426783864927
58-59	9.662945106514075E-4
60-61	4.5472682854183884E-4
62-63	0.001591543899896436
64-65	0.0029557243855219523
66-67	5.115676821095687E-4
68-69	0.0011368170713545972
70-71	3.4104512140637914E-4
72-73	5.684085356772986E-4
74-75	5.684085356772986E-4
76-77	3.97885974974109E-4
78-79	1.1368170713545971E-4
80-81	5.684085356772986E-4
82-83	1.1368170713545971E-4
84-85	2.842042678386493E-4
86-87	1.7052256070318957E-4
88-89	5.6840853567729855E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	879649.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.66889255101829
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.98907054775425	15.382193277853428
2	11.277978256651322	5.338745110988015
3	5.3956604450437835	3.8312792194656247
4	3.383135746438541	3.2030030587185156
5	2.344673049097395	2.7747907233177336
6	1.7703906577647186	2.514191175117583
7	1.3901055924384838	2.3031581931397263
8	1.07024465104309	2.0265204519072775
9	0.8897958255306017	1.8954433608145587
>10	6.554225550030821	30.118727655603735
>50	0.6077838277917811	9.863433195556537
>100	0.2981309296737855	12.413860690836119
>500	0.022563854580785702	3.6161641643340716
>1k	0.005280902135928568	2.590311660701342
>5k	4.8008201235714255E-4	0.9891499827340402
>10k+	4.8008201235714255E-4	1.1390280789116622
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10024	1.1395454323258483	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8705	0.9895992606141768	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4963	0.5642023125132866	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	3143	0.3573016055267499	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT	2174	0.2471440313124894	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1932	0.21963305818570814	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	1871	0.21269847405044512	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1867	0.2122437472219033	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1693	0.1924631301803333	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1692	0.19234944847319782	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	1328	0.15096930707589049	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	1082	0.1230036071205674	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	1051	0.11947947419936816	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	991	0.11265857177124058	No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	979	0.11129439128561507	TruSeq Adapter, Index 3 (95% over 21bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	927	0.10538294251457114	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	5.6840853567729855E-5	0.0
14-15	0.0	0.0	0.0	2.842042678386493E-4	0.0
16-17	0.0	0.0	0.0	5.684085356772986E-4	0.0
18-19	0.0	0.0	0.0	0.0010231353642191374	0.0
20-21	0.0	0.0	0.0	0.0016483847534641658	0.0
22-23	0.0	0.0	0.0	0.003580973774766981	0.0
24-25	0.0	0.0	0.0	0.009208218277972236	0.0
26-27	0.0	0.0	0.0	0.01864379997021539	0.0
28-29	0.0	0.0	0.0	0.044506388343532474	0.0
30-31	0.0	0.0	0.0	0.11010073336069273	0.0
32-33	0.0	0.0	0.0	0.21105008929698094	0.0
34-35	0.0	0.0	0.0	0.35087858907359637	0.0
36-37	0.0	0.0	0.0	0.563690744831177	0.0
38-39	0.0	0.0	0.0	0.8679598339792349	0.0
40-41	0.0	0.0	0.0	1.2174742425672056	0.0
42-43	0.0	0.0	0.0	1.5132172036801044	0.0
44-45	0.0	0.0	0.0	1.8355616842627003	0.0
46-47	0.0	0.0	0.0	2.2098587050062015	0.0
48-49	0.0	0.0	0.0	2.6048457964483562	0.0
50-51	0.0	0.0	0.0	3.023819728096093	0.0
52-53	0.0	0.0	0.0	3.4005609055430064	0.0
54-55	0.0	0.0	0.0	3.796002723813703	0.0
56-57	0.0	0.0	0.0	4.252832663937548	0.0
58-59	0.0	0.0	0.0	4.727453791228092	0.0
60-61	0.0	0.0	0.0	5.234872091027217	0.0
62-63	0.0	0.0	0.0	5.742063027412071	0.0
64-65	0.0	0.0	0.0	6.244479332097234	0.0
66-67	0.0	0.0	0.0	6.771962453205767	0.0
68-69	0.0	0.0	0.0	7.394369799772409	0.0
70-71	0.0	0.0	0.0	8.078676835874308	0.0
72-73	0.0	0.0	0.0	8.749342067119954	0.0
74-75	0.0	0.0	0.0	9.422110409947605	0.0
76-77	0.0	0.0	0.0	10.113579393599037	0.0
78-79	0.0	0.0	0.0	10.892696973451912	0.0
80-81	0.0	0.0	0.0	11.720811369080167	0.0
82-83	0.0	0.0	0.0	12.585986001234584	0.0
84-85	0.0	0.0	0.0	13.432971560247326	0.0
86-87	0.0	0.0	0.0	14.265007974771756	0.0
88	0.0	0.0	0.0	14.926862873714402	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCA	20	0.0021608088	70.51646	2
CGGTATA	30	1.3694003E-4	62.6813	2
GCGTATC	25	0.00522609	56.426003	1
TATAACG	35	2.9337464E-4	53.726826	2
CTACCGT	35	4.6310306E-6	40.29283	26-27
GCGTAGA	50	0.0017027105	37.59595	94
ACGTGCG	95	9.4552706E-7	34.62785	9
GGTATCA	8465	0.0	33.05126	1
CGTGACT	30	0.0041637686	31.329962	70-71
GTATCAA	12610	0.0	29.53304	1
TACCGTA	40	4.7769613E-4	29.380186	28-29
GCGTTCG	40	4.7849186E-4	29.371838	78-79
AACCCGC	75	3.6938218E-8	28.20498	28-29
AACCGTG	135	4.634676E-7	27.850437	8
TAAGGAC	155	5.0691597E-8	27.296692	4
CGGGAGT	380	0.0	27.21688	4
GTCGGGA	365	0.0	27.04741	2
TAATCGA	35	0.008841912	26.854252	92-93
GTGGGTA	410	0.0	26.363014	9
GTACATG	8095	0.0	25.965023	1
>>END_MODULE