FastQCFastQC Report
Wed 25 May 2016
SRR1033172_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033172_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3430296
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC36350.032.4421774
GGTATCA25350.032.3278921
ATCAACG37000.031.7508223
CAACGCA38150.030.7878825
GTATCAA40950.030.2487851
GTACATG90500.029.612141
AACGCAG40850.028.7420446
TACATGG88950.027.3252452
ACATGGG89900.025.9784583
TATCAAC47600.025.2843912
ACGCAGA49150.023.506147
CGCAGAG49650.022.9863178
CATGGGG58400.020.9975384
GTACGAC2151.1975517E-619.6707423
GAGTACA64350.019.2492261
TAGTGCG1508.3356054E-418.7863469
TAGACCG1300.00805955918.0701185
TACACCG3401.033186E-917.9638235
TACGACC2654.4996523E-717.7294314
CCGATCG1600.001285540817.6122029
Produced by FastQC (version 0.11.5)