##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033172_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3430296
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.717446541056514	33.0	31.0	34.0	30.0	34.0
2	31.829619659644532	34.0	31.0	34.0	30.0	34.0
3	31.98827623038945	34.0	31.0	34.0	30.0	34.0
4	35.321070543183446	37.0	35.0	37.0	33.0	37.0
5	35.43595246590965	37.0	35.0	37.0	35.0	37.0
6	35.50312596930411	37.0	37.0	37.0	35.0	37.0
7	35.44494702497977	37.0	37.0	37.0	35.0	37.0
8	35.430464892825576	37.0	37.0	37.0	35.0	37.0
9	37.102716208747	39.0	38.0	39.0	35.0	39.0
10-11	37.09498189077561	39.0	38.0	39.0	35.0	39.0
12-13	37.04416849158207	39.0	38.0	39.0	35.0	39.0
14-15	38.518851580155186	41.0	39.0	41.0	35.0	41.0
16-17	38.53677977060872	41.0	39.0	41.0	35.0	41.0
18-19	38.5420567204696	41.0	39.0	41.0	35.0	41.0
20-21	38.57614109686161	41.0	39.0	41.0	34.5	41.0
22-23	38.546078385072306	41.0	39.0	41.0	34.0	41.0
24-25	38.547900239512856	41.0	39.0	41.0	34.0	41.0
26-27	38.50733362368729	41.0	39.0	41.0	34.0	41.0
28-29	38.4755496318685	40.5	39.0	41.0	34.0	41.0
30-31	38.47619899273998	41.0	39.0	41.0	34.0	41.0
32-33	38.540946466427386	41.0	39.0	41.0	34.0	41.0
34-35	38.510599522606796	41.0	39.0	41.0	34.0	41.0
36-37	38.44350487538102	41.0	39.0	41.0	34.0	41.0
38-39	38.373313556614356	41.0	39.0	41.0	34.0	41.0
40-41	38.32149178962982	41.0	39.0	41.0	34.0	41.0
42-43	38.22224933358521	41.0	38.5	41.0	33.0	41.0
44-45	38.12562443590873	40.0	38.0	41.0	33.0	41.0
46-47	38.017693079547655	40.0	38.0	41.0	33.0	41.0
48-49	37.91508123497214	40.0	38.0	41.0	33.0	41.0
50-51	37.81195646090016	40.0	38.0	41.0	33.0	41.0
52-53	37.68299470366405	40.0	38.0	41.0	32.5	41.0
54-55	37.548101388335	40.0	37.0	41.0	32.0	41.0
56-57	37.409373126983795	40.0	37.0	41.0	32.0	41.0
58-59	37.232107229230365	40.0	37.0	41.0	31.5	41.0
60-61	37.02722024571641	40.0	36.0	41.0	31.0	41.0
62-63	36.78436336106272	39.0	36.0	41.0	31.0	41.0
64-65	36.528387637684915	39.0	35.0	41.0	31.0	41.0
66-67	36.249554994671016	39.0	35.0	41.0	31.0	41.0
68-69	35.951533191304776	38.0	35.0	40.0	30.5	41.0
70-71	35.60744743310781	37.0	35.0	40.0	30.0	41.0
72-73	35.24263153966888	37.0	35.0	39.5	30.0	41.0
74-75	34.86494387073302	36.5	34.5	39.0	29.5	41.0
76-77	34.52278928698864	36.0	34.0	39.0	29.0	40.5
78-79	34.13514052431627	35.5	34.0	37.5	29.0	39.5
80-81	33.75523672009646	35.0	34.0	37.0	29.0	39.0
82-83	33.50377066585507	35.0	34.0	37.0	29.0	39.0
84-85	33.209661207079506	35.0	34.0	36.0	29.0	38.0
86-87	32.930057202060695	35.0	34.0	36.0	29.0	37.0
88-89	32.54413657013855	35.0	34.0	36.0	27.5	37.0
90-91	32.337353686095895	35.0	34.0	35.0	27.0	36.5
92-93	32.1458038898101	35.0	34.0	35.0	27.0	36.0
94-95	31.970747422379876	35.0	34.0	35.0	27.0	36.0
96-97	31.79132223574875	35.0	34.0	35.0	26.5	36.0
98-99	31.599924175639654	35.0	33.5	35.0	25.5	36.0
100	31.442097416666083	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2412831860349165
1101	2	0.20641382657639085
1101	3	0.2555681149557003
1101	4	0.2198389166556538
1101	5	0.15675574903905698
1101	6	0.19104630436727632
1101	7	0.19616180785082094
1101	8	0.09276827168780954
1101	9	0.19770578597670152
1101	10-11	0.2065922203268613
1101	12-13	0.21016947369044914
1101	14-15	0.22183930259309648
1101	16-17	0.16837005286871687
1101	18-19	0.23784139279316463
1101	20-21	0.18094503165261955
1101	22-23	0.1520980208230256
1101	24-25	0.12331207687584822
1101	26-27	0.11929962407401007
1101	28-29	0.22464718918483584
1101	30-31	0.24509100595216182
1101	32-33	0.20203253988039904
1101	34-35	0.18374427973151342
1101	36-37	0.23093218951500205
1101	38-39	0.21088037270257587
1101	40-41	0.22135150277582483
1101	42-43	0.2299518868072994
1101	44-45	0.14012123546014266
1101	46-47	0.12458635478079572
1101	48-49	0.12230418252848807
1101	50-51	0.07483722742912136
1101	52-53	0.08900445995507766
1101	54-55	-5.490736800339846E-4
1101	56-57	0.05269143254152908
1101	58-59	0.04873610407546636
1101	60-61	0.03507985590930218
1101	62-63	0.03551976635889531
1101	64-65	0.04543046417537511
1101	66-67	0.04305677542175346
1101	68-69	0.013825492643633197
1101	70-71	0.09798955103194373
1101	72-73	0.12150663249643401
1101	74-75	0.10866244577270123
1101	76-77	0.05750191468989385
1101	78-79	0.03597364100365752
1101	80-81	0.08767282975701818
1101	82-83	0.09003585006817616
1101	84-85	-0.03595753404437119
1101	86-87	0.08295534650556391
1101	88-89	0.2532583202284542
1101	90-91	0.27351547386539465
1101	92-93	0.27315248779295587
1101	94-95	0.30904052120980907
1101	96-97	0.27653436507039686
1101	98-99	0.30722630739404266
1101	100	0.3023846859173851
1102	1	0.22477419250039432
1102	2	0.16837099938262057
1102	3	0.20701756265930982
1102	4	0.17360941856711065
1102	5	0.10644480705219905
1102	6	0.1080256747317705
1102	7	0.11051710971999285
1102	8	0.10967188848044174
1102	9	0.14212424518428435
1102	10-11	0.18224559488423608
1102	12-13	0.2097153926869808
1102	14-15	0.22455255770645977
1102	16-17	0.22628409899030544
1102	18-19	0.19088805170830625
1102	20-21	0.22060753187282955
1102	22-23	0.18590789697474008
1102	24-25	0.1606458154027095
1102	26-27	0.1631208632615042
1102	28-29	0.1518493279019566
1102	30-31	0.14718523987214382
1102	32-33	0.20252097424785376
1102	34-35	0.21997320526399733
1102	36-37	0.2170014599904846
1102	38-39	0.15971323918250135
1102	40-41	0.14355170361087488
1102	42-43	0.1113625328267176
1102	44-45	0.17643824654130214
1102	46-47	0.16398525899656846
1102	48-49	0.14975670441774724
1102	50-51	0.12619836937017226
1102	52-53	0.08348193648027546
1102	54-55	0.09933685553441762
1102	56-57	0.037543360734183295
1102	58-59	-0.02565874820506764
1102	60-61	0.007984961347702324
1102	62-63	0.09164853862252187
1102	64-65	0.05845171040972019
1102	66-67	0.053621756131015275
1102	68-69	-0.013899874232670584
1102	70-71	0.030654315050753667
1102	72-73	0.07406860067993648
1102	74-75	0.14024948310336072
1102	76-77	0.08204364962286093
1102	78-79	0.18064270359725043
1102	80-81	0.17528690054256657
1102	82-83	0.11979775483008126
1102	84-85	0.09236703477687058
1102	86-87	0.0815362883162507
1102	88-89	0.1405491170503268
1102	90-91	0.19881034023962485
1102	92-93	0.23019272527159984
1102	94-95	0.25285515398115166
1102	96-97	0.2609302303347363
1102	98-99	0.29540324483568625
1102	100	0.33975850336889124
1103	1	0.08193429675420916
1103	2	0.12148454217165394
1103	3	0.16053177285724018
1103	4	0.1527847065014356
1103	5	0.09846494035461717
1103	6	0.1029580562344492
1103	7	0.09059537268029771
1103	8	0.12454475494357098
1103	9	0.132407996459591
1103	10-11	0.13067294754543468
1103	12-13	0.07577898700076702
1103	14-15	0.08574013385653956
1103	16-17	0.0688538581243705
1103	18-19	0.0731001398038913
1103	20-21	0.09510939876430768
1103	22-23	0.10001395639667976
1103	24-25	0.10750861350638985
1103	26-27	0.15159885666172102
1103	28-29	0.2227196285904256
1103	30-31	0.21007236415036346
1103	32-33	0.18323329274213762
1103	34-35	0.18290875811199214
1103	36-37	0.22501474410945832
1103	38-39	0.20998371744238398
1103	40-41	0.2734975353587501
1103	42-43	0.1589392252749775
1103	44-45	0.08242291168165394
1103	46-47	0.08998629760575483
1103	48-49	0.13672322678094417
1103	50-51	0.11859164608393513
1103	52-53	0.0804788313292093
1103	54-55	0.10515323780068542
1103	56-57	0.09488558459631946
1103	58-59	0.03617441409085842
1103	60-61	0.11176238979064124
1103	62-63	0.13978544798293768
1103	64-65	0.16363356737632984
1103	66-67	0.10757803717559256
1103	68-69	0.062353706129073316
1103	70-71	0.10448431027195682
1103	72-73	0.14537845256282367
1103	74-75	0.13698501741696845
1103	76-77	0.1017175852726524
1103	78-79	0.11445485626425977
1103	80-81	0.18996349036837756
1103	82-83	0.2052462507898838
1103	84-85	0.1141365950719404
1103	86-87	0.1262418157072176
1103	88-89	0.22717740813250487
1103	90-91	0.2373537378326276
1103	92-93	0.26422415174067027
1103	94-95	0.25877313374963506
1103	96-97	0.1862140008277322
1103	98-99	0.26363280052704496
1103	100	0.29624684271736257
1104	1	0.062272164540893016
1104	2	0.05847771745937891
1104	3	0.06911547359764825
1104	4	0.06635827816909767
1104	5	0.07803441125771826
1104	6	0.004979837579554669
1104	7	0.005952357236402861
1104	8	-0.010035663956799112
1104	9	0.048727913420641755
1104	10-11	0.06239761283562473
1104	12-13	0.05714067423097191
1104	14-15	0.04937857458330086
1104	16-17	0.07040522874811472
1104	18-19	0.06682633853025521
1104	20-21	0.02327421960920617
1104	22-23	0.0475998999666416
1104	24-25	0.04718374614657961
1104	26-27	0.10881463610896702
1104	28-29	0.1580573070008029
1104	30-31	0.09836219382429334
1104	32-33	0.0867745271275524
1104	34-35	0.08072065319547761
1104	36-37	0.1086375229046368
1104	38-39	0.07199687960684287
1104	40-41	0.060043280994648285
1104	42-43	0.0655436059069956
1104	44-45	0.02725511916437995
1104	46-47	-0.017261962435547673
1104	48-49	-0.1118378511694118
1104	50-51	-0.10680258930159425
1104	52-53	-0.14167139832793652
1104	54-55	-0.12481008461707432
1104	56-57	-0.12836595523770455
1104	58-59	-0.16434210208137046
1104	60-61	-0.1045114831388787
1104	62-63	-0.15185988399370842
1104	64-65	-0.1513747805355692
1104	66-67	-0.14563838103278925
1104	68-69	-0.16087299981867176
1104	70-71	-0.16081750948070805
1104	72-73	-0.14558027261736584
1104	74-75	-0.18648155152470025
1104	76-77	-0.24794981876731015
1104	78-79	-0.22331566265751235
1104	80-81	-0.168505857798408
1104	82-83	-0.11484211116656695
1104	84-85	-0.06674624651428473
1104	86-87	-0.06373440477182868
1104	88-89	0.054837750995730516
1104	90-91	0.04177979969623635
1104	92-93	-0.014985893187571264
1104	94-95	-0.01620376746043206
1104	96-97	0.029492749429163467
1104	98-99	0.009233507258915807
1104	100	0.11381621330220781
1105	1	0.13875388211196693
1105	2	0.14438801921494715
1105	3	0.1817523003771555
1105	4	0.1694542119346707
1105	5	0.16120048843531265
1105	6	0.16226914691002037
1105	7	0.1205410955975097
1105	8	0.1216375094125155
1105	9	0.15421507281087798
1105	10-11	0.18101101548559484
1105	12-13	0.1599598044328232
1105	14-15	0.15253780608135514
1105	16-17	0.18226442601778103
1105	18-19	0.18877785326086638
1105	20-21	0.1884077847696588
1105	22-23	0.23918189682159152
1105	24-25	0.21827731864876654
1105	26-27	0.20717385570150526
1105	28-29	0.2845688380023148
1105	30-31	0.29591620276335817
1105	32-33	0.30925067886352764
1105	34-35	0.30255281499162123
1105	36-37	0.28730688154384154
1105	38-39	0.22873813772368834
1105	40-41	0.22353239487600263
1105	42-43	0.19106300943996501
1105	44-45	0.20489524476771237
1105	46-47	0.2516741296735674
1105	48-49	0.2230307663505684
1105	50-51	0.2147732067566892
1105	52-53	0.14444486909010124
1105	54-55	0.144545578198489
1105	56-57	0.14359622486319523
1105	58-59	0.12932147741532418
1105	60-61	0.17443805229743248
1105	62-63	0.24507444212851226
1105	64-65	0.19220164056300604
1105	66-67	0.2181399380056419
1105	68-69	0.12776922413426206
1105	70-71	0.07167823677276886
1105	72-73	0.14682310656878883
1105	74-75	0.09932189810250236
1105	76-77	0.17417601275953132
1105	78-79	0.14499211512578825
1105	80-81	0.14014727955667894
1105	82-83	0.20699749158559655
1105	84-85	0.2017127314952134
1105	86-87	0.26791059217737967
1105	88-89	0.2032586313104332
1105	90-91	0.2104401795818447
1105	92-93	0.13855208297160715
1105	94-95	0.20526398591652395
1105	96-97	0.2274884535172248
1105	98-99	0.23159387771930895
1105	100	0.275993734670152
1106	1	0.10563944883735132
1106	2	0.11862828926255276
1106	3	0.062325357589742225
1106	4	0.0295270858055261
1106	5	0.0493836292363099
1106	6	0.10998870122310223
1106	7	0.01857885929118197
1106	8	0.07151675221209786
1106	9	0.064460818464255
1106	10-11	0.038858897723947905
1106	12-13	0.008848230499069132
1106	14-15	0.07078850205561338
1106	16-17	0.031039197141637942
1106	18-19	0.09194579899246946
1106	20-21	0.053473931720361634
1106	22-23	0.05233633227109635
1106	24-25	0.11103651485000654
1106	26-27	0.12049650648619803
1106	28-29	0.11384665971061025
1106	30-31	0.1135374411300063
1106	32-33	0.06051601903534731
1106	34-35	0.022784335433613023
1106	36-37	0.04283526978656482
1106	38-39	0.06527832399286382
1106	40-41	0.0999553628447174
1106	42-43	0.0861576538482467
1106	44-45	0.1321557994286664
1106	46-47	0.07525836743948133
1106	48-49	0.11022935545643975
1106	50-51	0.10463366573000599
1106	52-53	0.07863154578082998
1106	54-55	0.06291604364191272
1106	56-57	0.0975490782870807
1106	58-59	0.06094311952887921
1106	60-61	0.06096761810394469
1106	62-63	0.1079340350966902
1106	64-65	0.08120757090143371
1106	66-67	0.09695127776242884
1106	68-69	0.1075314198401287
1106	70-71	0.04546277702903012
1106	72-73	0.08747626220080917
1106	74-75	0.08737164815005372
1106	76-77	0.09627082705758738
1106	78-79	0.06438579488751373
1106	80-81	0.05767683764948117
1106	82-83	0.033538940269380646
1106	84-85	0.05368778949384989
1106	86-87	0.03719666567474178
1106	88-89	0.13412639930685089
1106	90-91	0.09657096535873677
1106	92-93	0.1496228758338205
1106	94-95	0.10324585342845438
1106	96-97	0.08988010645442657
1106	98-99	0.14521265973944253
1106	100	0.13080100384535598
1107	1	0.09938800691063676
1107	2	0.04438375138841977
1107	3	0.09789451920160985
1107	4	0.05257885473614721
1107	5	0.0802409325861646
1107	6	0.00411574266890824
1107	7	0.013565611477531547
1107	8	0.07853784267410191
1107	9	-0.01562902163055213
1107	10-11	-0.012293228589541627
1107	12-13	-0.030111375491323145
1107	14-15	-0.05781018878240474
1107	16-17	-0.04600964637940308
1107	18-19	0.018541304336736175
1107	20-21	0.022326057747264372
1107	22-23	0.03316834368900601
1107	24-25	0.01496400080331739
1107	26-27	0.0771998894433068
1107	28-29	0.0612515677783847
1107	30-31	0.0885894579645452
1107	32-33	0.033981898571234126
1107	34-35	0.014753788798302025
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2216	24-25	-0.294338650469804
2216	26-27	-0.3818691670745835
2216	28-29	-0.33628720785686994
2216	30-31	-0.3504629898021392
2216	32-33	-0.35019963354776706
2216	34-35	-0.3230176625308374
2216	36-37	-0.36357676657033977
2216	38-39	-0.3274904121655098
2216	40-41	-0.3635165771686104
2216	42-43	-0.389672872293076
2216	44-45	-0.34404848015602596
2216	46-47	-0.267293493782617
2216	48-49	-0.27052789209414385
2216	50-51	-0.25488526110062537
2216	52-53	-0.2793070905091142
2216	54-55	-0.2610143890261938
2216	56-57	-0.371965927492127
2216	58-59	-0.3428803445994646
2216	60-61	-0.306522285529347
2216	62-63	-0.21821368860632617
2216	64-65	-0.17165726730836894
2216	66-67	-0.18504263663643172
2216	68-69	-0.22917716825774193
2216	70-71	-0.2597838027798929
2216	72-73	-0.24620670429345637
2216	74-75	-0.21541504319727522
2216	76-77	-0.25262137211026925
2216	78-79	-0.26581415211126114
2216	80-81	-0.1932492655071627
2216	82-83	-0.2517694052120518
2216	84-85	-0.3325720995446062
2216	86-87	-0.3409086344768184
2216	88-89	-0.24903658492802094
2216	90-91	-0.2765991063089288
2216	92-93	-0.2799963270298278
2216	94-95	-0.34361646708026683
2216	96-97	-0.377173410239962
2216	98-99	-0.36694962025291744
2216	100	-0.3612074331188211
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	31104.0
3	5576.0
4	3449.0
5	2737.0
6	2399.0
7	2738.0
8	3236.0
9	3599.0
10	3930.0
11	4383.0
12	4766.0
13	5421.0
14	6142.0
15	6440.0
16	6434.0
17	6639.0
18	6844.0
19	6632.0
20	6699.0
21	7028.0
22	8111.0
23	10852.0
24	16355.0
25	23760.0
26	26516.0
27	22657.0
28	21956.0
29	28390.0
30	35096.0
31	41770.0
32	54497.0
33	75162.0
34	104248.0
35	156474.0
36	284863.0
37	634568.0
38	1167571.0
39	589617.0
40	1637.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.64347066122012	20.168169967024692	13.410542855321065	23.77781651643413
2	14.915669676486889	20.235775250282455	41.44784251140248	23.40071256182818
3	17.876210384820258	25.799888099387346	30.380170371308168	25.94373114448423
4	11.718450846222874	16.938630942538268	40.08733886798351	31.25557934325534
5	12.271805007625092	38.5959739396543	36.5467504624781	12.585470590242506
6	30.23070715363606	39.2021401899022	18.119222991647753	12.44792966481398
7	26.673119392433108	32.99038650758365	23.185586320998812	17.150907778984426
8	24.256091480819524	37.63577361879299	20.774799623300552	17.333335277086938
9	24.60565689445492	17.155102310966207	21.503832269437684	36.73540852514119
10-11	23.25373965120061	27.408261311312266	29.736297422019618	19.601701615467505
12-13	23.902168081731666	24.781427855010264	29.642409381414442	21.673994681843627
14-15	21.126082343934947	26.874479812307463	26.504427636452277	25.49501020730532
16-17	20.916409094812245	30.3563319085422	27.11325186962833	21.61400712701722
18-19	20.86107134739868	29.447336152001036	28.926070693538918	20.765521807061365
20-21	21.98956513016761	28.682610076271413	28.436784417852007	20.891040375708965
22-23	21.52979311340167	28.47689099365775	28.527798086027445	21.46551780691313
24-25	21.42741962997231	28.630782648606072	28.467999973173296	21.47379774824832
26-27	21.20280269177607	28.63231807848744	28.49532085298377	21.669558376752722
28-29	21.24061550698677	28.648841481540227	28.591751282670693	21.518791728802313
30-31	21.511906431534484	29.086673526519142	28.218529940806803	21.182890101139574
32-33	21.179727348117527	28.681925965496287	28.732400855186345	21.405945831199837
34-35	21.25981382255632	28.50122857286556	28.755690030810904	21.48326757376722
36-37	21.567135133244022	28.7432015008812	28.271158620227204	21.418504745647578
38-39	21.432216936126355	28.93000656732824	28.390523513003092	21.24725298354232
40-41	21.187140633901244	28.695308277147685	28.68348611281989	21.434064976131182
42-43	21.189458258583244	28.51004237413523	28.664617808448618	21.635881558832907
44-45	21.328666894361834	28.657171399213038	28.61001430164712	21.404147404778012
46-47	21.35643849981917	28.87365447468117	28.46623779728816	21.303669228211504
48-49	21.345972199547543	28.647949366793384	28.531701145136086	21.474377288522984
50-51	21.286863153537013	28.597206774759226	28.554788000880443	21.561142070823315
52-53	21.348051073809156	28.56402760829963	28.548167806614206	21.539753511277006
54-55	21.416130486604814	28.951915868621903	28.358336444176174	21.273617200597105
56-57	21.25103564967084	28.738454801945302	28.369002633654926	21.641506914728936
58-59	21.134565278390188	28.406665767791356	28.750303370418013	21.708465583400447
60-61	21.34619889297726	28.719125448243926	28.471719436072302	21.462956222706516
62-63	21.22962649194126	28.76730519218808	28.419441257493506	21.58362705837715
64-65	21.223462854920605	28.494122421207386	28.59321172664645	21.689202997225557
66-67	21.102947939410132	28.59426743320117	28.45560380771802	21.847180819670672
68-69	21.466807455429688	28.735761026039896	28.394410468433602	21.403021050096815
70-71	21.348934016027187	28.533544864061046	28.506228947321237	21.61129217259053
72-73	21.211486926234915	28.647160299657674	28.585940227345112	21.555412546762295
74-75	21.237687587776612	28.704553852477716	28.456902639839488	21.600855919906188
76-77	21.265159721046995	28.693945965281447	28.353920424373797	21.686973889297757
78-79	21.373413117022764	28.74214165593962	28.449767308909724	21.434677918127896
80-81	21.36352067924152	28.459243672123236	28.596991585570315	21.580244063064928
82-83	21.149842437518338	28.605035265163973	28.764587411356075	21.480534885961614
84-85	21.246039058501754	28.491389821653957	28.50688448401244	21.75568663583185
86-87	21.33953757117586	28.63313194777269	28.419152501460545	21.60817797959091
88-89	21.179909276328253	28.445355500584547	28.55776286899387	21.816972354093327
90-91	21.13819385349447	28.433036799891116	28.637785383004516	21.7909839636099
92-93	21.33480446213723	28.556012183011514	28.473528847974443	21.635654506876815
94-95	21.10713009401102	28.352866354308436	28.649377422075297	21.890626129605252
96-97	21.220357444671947	28.339116537216064	28.609709770317625	21.830816247794363
98-99	21.17265926164997	28.408277246312142	28.542757057550645	21.876306434487244
100	21.378725006664695	28.28625131570882	28.425850624633586	21.909173052992895
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	359.0
1	335.0
2	323.0
3	291.0
4	215.0
5	184.5
6	184.5
7	174.0
8	184.5
9	204.0
10	215.0
11	236.0
12	260.0
13	277.5
14	311.5
15	398.0
16	548.0
17	795.0
18	1140.0
19	1664.0
20	2449.5
21	3611.5
22	5488.0
23	8784.5
24	13001.5
25	18199.5
26	25595.0
27	34553.0
28	45449.0
29	56878.0
30	66915.0
31	77107.5
32	87759.5
33	98219.5
34	107899.5
35	115557.0
36	120705.5
37	125182.0
38	128320.5
39	129054.5
40	129056.0
41	128476.0
42	126702.0
43	125131.0
44	121840.0
45	117645.0
46	114189.0
47	108516.5
48	104389.0
49	101956.0
50	98474.5
51	93949.0
52	87323.0
53	82640.0
54	79263.5
55	75012.5
56	69802.0
57	63449.0
58	56334.5
59	49122.5
60	42510.0
61	35781.0
62	30109.5
63	24809.5
64	19873.5
65	15738.0
66	12236.0
67	9300.5
68	7024.0
69	5580.5
70	4296.0
71	2992.5
72	2248.0
73	1712.5
74	1283.5
75	936.5
76	582.5
77	399.5
78	267.0
79	155.0
80	108.0
81	67.0
82	32.5
83	25.0
84	19.5
85	12.5
86	13.5
87	12.5
88	7.5
89	7.0
90	9.0
91	4.5
92	0.5
93	1.0
94	2.5
95	3.0
96	1.0
97	1.0
98	3.5
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3315748844997633
2	0.11856119705121657
3	0.06570861523320437
4	0.039442660341848054
5	0.04329072476544298
6	0.009328641026896803
7	0.006442592709200605
8	0.014750913623780572
9	0.017636961941476768
10-11	0.021324690347421914
12-13	0.015625473720052147
14-15	0.018788466068234345
16-17	0.01954641815166971
18-19	0.019284050122788235
20-21	0.024283618673140744
22-23	0.015115313663893728
24-25	0.025566306814339055
26-27	0.019371506132415395
28-29	0.018526098039352873
30-31	0.019794210178946656
32-33	0.025303938785457582
34-35	0.01467803361575794
36-37	0.009066272998015332
38-39	0.012783153407169527
40-41	0.00868729695629765
42-43	0.007448336819912917
44-45	0.007914768871257758
46-47	0.007856464864839652
48-49	0.003964672436431142
50-51	0.00615107267711008
52-53	0.007011056771777129
54-55	0.0026091042872101997
56-57	0.0023904642631423063
58-59	0.002434192267955885
60-61	0.0030463843353459876
62-63	0.0022009762422834644
64-65	0.0016616641829159933
66-67	0.001530480168475257
68-69	0.0028568963144871462
70-71	0.0017928481973567298
72-73	0.0014138721556390468
74-75	0.001996912219820097
76-77	0.003279600361018408
78-79	0.0030463843353459876
80-81	0.003410784375459144
82-83	0.0020260642230291496
84-85	0.0024050402647468323
86-87	0.0017491201925431508
88-89	0.6070030108188914
90-91	0.6191010921506482
92-93	0.6157631877832117
94-95	0.6017702262428666
96-97	0.5995255219957695
98-99	0.5973391217550905
100	0.5986946899043115
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3430296.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.19116407820876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.09705803250517	48.847587380335135
2	14.356546630935613	18.43126880765686
3	4.627352585956124	8.911054472784995
4	1.8727779500375474	4.808631866916467
5	0.9935764009070918	3.1889412887431625
6	0.55651145446054	2.143387085080747
7	0.35600432479027155	1.599663241761497
8	0.22680736252585434	1.1647222897634315
9	0.1665348140416869	0.962105721559552
>10	0.7092790808578772	7.6842473312706066
>50	0.02802017695146638	1.224550343394268
>100	0.009440129393475397	1.0016340469244231
>500	9.105663736596963E-5	0.03220612380894565
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.915200320905251E-5	0.0
5	0.0	0.0	0.0	2.915200320905251E-5	0.0
6	0.0	0.0	0.0	2.915200320905251E-5	0.0
7	0.0	0.0	0.0	5.830400641810502E-5	0.0
8	0.0	0.0	0.0	1.1660801283621005E-4	0.0
9	0.0	0.0	0.0	1.4576001604526257E-4	0.0
10-11	0.0	0.0	0.0	1.4576001604526257E-4	0.0
12-13	0.0	0.0	0.0	1.4576001604526257E-4	0.0
14-15	0.0	0.0	0.0	2.915200320905251E-4	0.0
16-17	0.0	0.0	0.0	4.2270404653126147E-4	0.0
18-19	0.0	0.0	0.0	4.518560497403139E-4	0.0
20-21	0.0	0.0	0.0	5.247360577629453E-4	0.0
22-23	0.0	0.0	0.0	5.538880609719978E-4	0.0
24-25	0.0	0.0	0.0	6.704960738082078E-4	0.0
26-27	0.0	0.0	0.0	8.454080930625229E-4	0.0
28-29	0.0	0.0	0.0	0.0015013281652662045	0.0
30-31	0.0	0.0	0.0	0.005189056571211347	0.0
32-33	0.0	0.0	0.0	0.01339534547455963	0.0
34-35	0.0	0.0	0.0	0.023919218633027588	0.0
36-37	0.0	0.0	0.0	0.04227040465312615	0.0
38-39	0.0	0.0	0.0	0.07486234424084685	0.0
40-41	0.0	0.0	0.0	0.11499007665810765	0.0
42-43	0.0	0.0	0.0	0.15568627313794495	0.0
44-45	0.0	0.0	0.0	0.20120712614888042	0.0
46-47	0.0	1.4576001604526256E-5	0.0	0.2593070685445221	0.0
48-49	0.0	2.915200320905251E-5	0.0	0.32530720380981704	0.0
50-51	0.0	2.915200320905251E-5	0.0	0.39780823579073055	0.0
52-53	0.0	2.915200320905251E-5	0.0	0.46877878760316893	0.0
54-55	0.0	2.915200320905251E-5	0.0	0.5472851322451473	0.0
56-57	0.0	2.915200320905251E-5	0.0	0.6430348867852804	0.0
58-59	0.0	2.915200320905251E-5	0.0	0.7513491547085149	0.0
60-61	0.0	2.915200320905251E-5	0.0	0.8676947995158435	0.0
62-63	0.0	2.915200320905251E-5	0.0	0.9819706520953293	0.0
64-65	0.0	2.915200320905251E-5	0.0	1.1040883935380503	0.0
66-67	0.0	5.830400641810502E-5	0.0	1.2447759610249378	0.0
68-69	0.0	5.830400641810502E-5	0.0	1.401744922304081	0.0
70-71	0.0	5.830400641810502E-5	0.0	1.5753742534171977	1.4576001604526256E-5
72-73	0.0	5.830400641810502E-5	0.0	1.759512881687178	2.915200320905251E-5
74-75	0.0	5.830400641810502E-5	0.0	1.9441325180101074	2.915200320905251E-5
76-77	0.0	5.830400641810502E-5	0.0	2.140850235664794	2.915200320905251E-5
78-79	0.0	5.830400641810502E-5	0.0	2.3693727888205567	2.915200320905251E-5
80-81	0.0	5.830400641810502E-5	0.0	2.6224121766751325	2.915200320905251E-5
82-83	0.0	5.830400641810502E-5	0.0	2.88524663760795	2.915200320905251E-5
84-85	0.0	5.830400641810502E-5	0.0	3.147206538444496	2.915200320905251E-5
86-87	0.0	5.830400641810502E-5	0.0	3.422663816766833	2.915200320905251E-5
88	0.0	5.830400641810502E-5	0.0	3.6497433457637474	2.915200320905251E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	3635	0.0	32.442177	4
GGTATCA	2535	0.0	32.327892	1
ATCAACG	3700	0.0	31.750822	3
CAACGCA	3815	0.0	30.787882	5
GTATCAA	4095	0.0	30.248785	1
GTACATG	9050	0.0	29.61214	1
AACGCAG	4085	0.0	28.742044	6
TACATGG	8895	0.0	27.325245	2
ACATGGG	8990	0.0	25.978458	3
TATCAAC	4760	0.0	25.284391	2
ACGCAGA	4915	0.0	23.50614	7
CGCAGAG	4965	0.0	22.986317	8
CATGGGG	5840	0.0	20.997538	4
GTACGAC	215	1.1975517E-6	19.670742	3
GAGTACA	6435	0.0	19.249226	1
TAGTGCG	150	8.3356054E-4	18.786346	9
TAGACCG	130	0.008059559	18.070118	5
TACACCG	340	1.033186E-9	17.963823	5
TACGACC	265	4.4996523E-7	17.729431	4
CCGATCG	160	0.0012855408	17.612202	9
>>END_MODULE