FastQCFastQC Report
Wed 25 May 2016
SRR1033172_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033172_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3430296
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59560.1736293311131168No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49580.14453563191048235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47450.052.0623321
GTATCAA85300.042.6413571
ATCAACG100200.035.519043
TCAACGC101400.035.1450654
CAACGCA103450.034.4481165
AACGCAG107700.033.249776
TATCAAC107750.033.1179852
ACGCAGA122650.028.8137477
CGCAGAG122150.028.7765398
GTACATG88400.026.1886671
GCAGAGT138700.025.1734529
TACATGG89050.025.1311552
ACATGGG89800.023.6120283
GAGTACT97200.021.41745812-13
AGAGTAC130400.020.6674210-11
CAGAGTA139200.020.55930910-11
CATGGGG57300.019.7740444
GTGGTAT23500.019.6227021
GTATAGA13900.018.9571821
AGTACTT106450.018.8721312-13
Produced by FastQC (version 0.11.5)