##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033172_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3430296 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.762674124915165 34.0 31.0 34.0 31.0 34.0 2 32.85838918857148 34.0 31.0 34.0 31.0 34.0 3 32.96150827800283 34.0 31.0 34.0 31.0 34.0 4 36.38592762840291 37.0 37.0 37.0 35.0 37.0 5 36.3197365475166 37.0 37.0 37.0 35.0 37.0 6 36.401812554951526 37.0 37.0 37.0 35.0 37.0 7 36.379225874385185 37.0 37.0 37.0 35.0 37.0 8 36.37326866252941 37.0 37.0 37.0 35.0 37.0 9 38.19190093216446 39.0 39.0 39.0 37.0 39.0 10-11 38.18042305970097 39.0 39.0 39.0 37.0 39.0 12-13 38.14109350913157 39.0 39.0 39.0 37.0 39.0 14-15 39.68523459783063 41.0 40.0 41.0 37.0 41.0 16-17 39.698048215081144 41.0 40.0 41.0 37.0 41.0 18-19 39.714959437902735 41.0 40.0 41.0 37.0 41.0 20-21 39.71178434747322 41.0 40.0 41.0 37.0 41.0 22-23 39.64500395884204 41.0 40.0 41.0 37.0 41.0 24-25 39.598712035346225 41.0 40.0 41.0 37.0 41.0 26-27 39.50185946052469 41.0 39.5 41.0 36.5 41.0 28-29 39.41347219015502 41.0 39.0 41.0 36.0 41.0 30-31 39.32089461084408 41.0 39.0 41.0 36.0 41.0 32-33 39.35662053653679 41.0 39.0 41.0 36.0 41.0 34-35 39.301980936922064 41.0 39.0 41.0 35.5 41.0 36-37 39.21795655535266 41.0 39.0 41.0 35.0 41.0 38-39 39.13096624897676 41.0 39.0 41.0 35.0 41.0 40-41 39.01978240361765 41.0 39.0 41.0 35.0 41.0 42-43 38.91140997744801 41.0 39.0 41.0 35.0 41.0 44-45 38.776268432811634 41.0 39.0 41.0 35.0 41.0 46-47 38.58060747527327 41.0 39.0 41.0 35.0 41.0 48-49 38.411054323008855 41.0 39.0 41.0 35.0 41.0 50-51 38.29203908350766 41.0 38.5 41.0 34.5 41.0 52-53 38.1721211522271 40.5 38.0 41.0 34.0 41.0 54-55 38.05009276167421 40.0 38.0 41.0 34.0 41.0 56-57 37.90666286524545 40.0 38.0 41.0 34.0 41.0 58-59 37.73317973142842 40.0 37.0 41.0 34.0 41.0 60-61 37.55510588590606 40.0 37.0 41.0 33.5 41.0 62-63 37.336325786462744 40.0 36.0 41.0 33.0 41.0 64-65 37.09923575108387 39.0 36.0 41.0 33.0 41.0 66-67 36.82780115768435 39.0 35.0 41.0 33.0 41.0 68-69 36.51344330051984 39.0 35.0 41.0 32.0 41.0 70-71 36.185790818051856 37.5 35.0 40.0 32.0 41.0 72-73 35.83343828054488 37.0 35.0 40.0 32.0 41.0 74-75 35.494309091693545 37.0 35.0 39.0 31.0 41.0 76-77 35.14327072649124 36.0 35.0 39.0 31.0 41.0 78-79 34.78787646896944 36.0 35.0 38.5 31.0 40.0 80-81 34.43206825300207 35.0 35.0 37.0 31.0 39.0 82-83 34.0917133681758 35.0 35.0 37.0 31.0 39.0 84-85 33.80091353632456 35.0 34.0 36.5 30.5 38.0 86-87 33.55273757716535 35.0 34.0 36.0 30.0 37.0 88-89 33.3206013416918 35.0 34.0 36.0 30.0 37.0 90-91 33.10863391963842 35.0 34.0 35.5 30.0 36.5 92-93 32.93611221888723 35.0 34.0 35.0 30.0 36.0 94-95 32.781753819495464 35.0 34.0 35.0 30.0 36.0 96-97 32.65562476241118 35.0 34.0 35.0 29.5 36.0 98-99 32.54845441909386 35.0 34.0 35.0 29.5 36.0 100 32.44896183886172 35.0 34.0 35.0 29.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14537695464283473 1101 2 0.10092219798141855 1101 3 0.1563966937806356 1101 4 0.07153765112699517 1101 5 0.04515284107963424 1101 6 0.09745370312526802 1101 7 0.11908994636633707 1101 8 0.11282283370268686 1101 9 0.120788286725805 1101 10-11 0.14016704170122551 1101 12-13 0.11621972415019854 1101 14-15 0.134672411777629 1101 16-17 0.13056175047303498 1101 18-19 0.13433000812745632 1101 20-21 0.15171175987583752 1101 22-23 0.1879269251733291 1101 24-25 0.09245994737278096 1101 26-27 0.13334103216035942 1101 28-29 0.1826457963200312 1101 30-31 0.148844016014678 1101 32-33 0.09529120327312768 1101 34-35 0.09615477301002073 1101 36-37 0.1778634283291538 1101 38-39 0.13338123548796688 1101 40-41 0.13219860243295756 1101 42-43 0.132320936691535 1101 44-45 0.11938892678361412 1101 46-47 0.16207012320584369 1101 48-49 0.1481863384446882 1101 50-51 0.07502900523169131 1101 52-53 0.11537496342998566 1101 54-55 0.08338509911661163 1101 56-57 0.08420877636444146 1101 58-59 0.07499497174906367 1101 60-61 0.07400802445845045 1101 62-63 0.05986407059224774 1101 64-65 0.117714128197008 1101 66-67 0.09851485218713663 1101 68-69 0.10482287204324336 1101 70-71 0.04136991579630944 1101 72-73 0.06711029146678982 1101 74-75 0.05501873051829875 1101 76-77 0.04487724026625273 1101 78-79 0.05637557168110874 1101 80-81 0.022283246166175275 1101 82-83 0.10302947948168395 1101 84-85 0.15439850043770065 1101 86-87 0.10114860137096571 1101 88-89 0.09519414951198257 1101 90-91 0.09776769394095908 1101 92-93 0.11397634645154398 1101 94-95 0.15803337321439415 1101 96-97 0.17503622756421322 1101 98-99 0.24048530354174602 1101 100 0.29172230390689435 1102 1 0.11409953801580741 1102 2 0.11229712531083891 1102 3 0.15276756917482004 1102 4 0.09633461393728027 1102 5 0.05463525039556316 1102 6 0.10644943978471133 1102 7 0.11235844873584 1102 8 0.1570501472696222 1102 9 0.18046932895401824 1102 10-11 0.17761683293847597 1102 12-13 0.1967754999196245 1102 14-15 0.24967051793832695 1102 16-17 0.2202786435195634 1102 18-19 0.20367557654351032 1102 20-21 0.22155501164195357 1102 22-23 0.2295213616900753 1102 24-25 0.21978364414709262 1102 26-27 0.2605638709814144 1102 28-29 0.25670063838263957 1102 30-31 0.22053948665576684 1102 32-33 0.215247204541555 1102 34-35 0.2294209411124868 1102 36-37 0.2561157670004661 1102 38-39 0.24343642108421193 1102 40-41 0.28127194246079057 1102 42-43 0.29693322814899403 1102 44-45 0.3289750568331229 1102 46-47 0.33749909982794435 1102 48-49 0.3106788511730514 1102 50-51 0.33666840942102993 1102 52-53 0.3488734571671017 1102 54-55 0.30913030285849175 1102 56-57 0.32116198532097684 1102 58-59 0.32533300004214283 1102 60-61 0.34273611317821207 1102 62-63 0.36651166673761537 1102 64-65 0.2963088994388343 1102 66-67 0.33696441440195457 1102 68-69 0.3900458606097388 1102 70-71 0.39650948734820446 1102 72-73 0.362651841420103 1102 74-75 0.34919561510861286 1102 76-77 0.33215944874712733 1102 78-79 0.3366190841804553 1102 80-81 0.31005454965612245 1102 82-83 0.2574108546732674 1102 84-85 0.31659240315602233 1102 86-87 0.34531557633705035 1102 88-89 0.30599806227205306 1102 90-91 0.3111137556360788 1102 92-93 0.31392481640556014 1102 94-95 0.333980292862762 1102 96-97 0.36504358270753556 1102 98-99 0.3177744267381968 1102 100 0.32739871283524735 1103 1 0.10993596219370261 1103 2 0.133892295778125 1103 3 0.10617612480340455 1103 4 0.07310508600479437 1103 5 0.037787446357882004 1103 6 0.09259609293921045 1103 7 0.08402624070480869 1103 8 0.12850176161934712 1103 9 0.12394793518490843 1103 10-11 0.062425956471038546 1103 12-13 0.09147754677405828 1103 14-15 0.09402470331554724 1103 16-17 0.15472120036014303 1103 18-19 0.1350526775170735 1103 20-21 0.1155881975969777 1103 22-23 0.1384140684926649 1103 24-25 0.09067284222719962 1103 26-27 0.1266102974953327 1103 28-29 0.09143573111651904 1103 30-31 0.1636764024220838 1103 32-33 0.12917445406783656 1103 34-35 0.13081078554744607 1103 36-37 0.11143003382651528 1103 38-39 0.06142201892295418 1103 40-41 0.13706507132648227 1103 42-43 0.06837499877267561 1103 44-45 0.14043496895276775 1103 46-47 0.04875043599097495 1103 48-49 -0.05349824962969052 1103 50-51 -0.05968328363578479 1103 52-53 -0.03055324081158517 1103 54-55 -0.062013380095805815 1103 56-57 -0.04351063284964596 1103 58-59 0.0033968224058043006 1103 60-61 -0.04747059652752483 1103 62-63 -0.11123704962768954 1103 64-65 -0.07963437934616024 1103 66-67 -0.05283456005687981 1103 68-69 -0.05083948966249352 1103 70-71 0.07100625087628032 1103 72-73 0.03714992883324442 1103 74-75 0.011717389814975832 1103 76-77 -0.02636773765973288 1103 78-79 0.026694814676744727 1103 80-81 0.05223228934017499 1103 82-83 0.036151801541493 1103 84-85 0.041943264727613894 1103 86-87 0.02034383701257525 1103 88-89 0.009426476176599863 1103 90-91 -0.01770498015618216 1103 92-93 0.01209971272614041 1103 94-95 -0.015122988535850368 1103 96-97 -0.013211327518114047 1103 98-99 -0.037907474429083265 1103 100 0.01884176036741536 1104 1 0.03851153510818506 1104 2 0.07007911703043845 1104 3 0.06671129198172565 1104 4 0.017176348817130815 1104 5 -0.043496709192737626 1104 6 -0.008765839968347677 1104 7 0.020627888176228737 1104 8 0.03256798804583383 1104 9 0.09558010294978203 1104 10-11 0.09849133121602449 1104 12-13 0.11197803249866212 1104 14-15 0.09741226651348711 1104 16-17 0.09893681909345275 1104 18-19 0.08849780087270176 1104 20-21 0.12905340507663254 1104 22-23 0.11410128436424571 1104 24-25 0.08149144942084519 1104 26-27 0.09798188347575376 1104 28-29 0.13178033455743332 1104 30-31 0.10446793392355147 1104 32-33 0.08214300429504107 1104 34-35 0.09155414381361737 1104 36-37 0.18437972831647897 1104 38-39 0.1139258704273658 1104 40-41 0.20118682083589334 1104 42-43 0.18806235386141879 1104 44-45 0.2086563774824839 1104 46-47 0.16696835385489095 1104 48-49 0.18435109077778833 1104 50-51 0.16888410003343068 1104 52-53 0.14551325995462605 1104 54-55 0.11193773625186765 1104 56-57 0.13616583054289322 1104 58-59 0.11221436704487076 1104 60-61 0.1316279518263812 1104 62-63 0.08212421585638197 1104 64-65 0.09329940753487875 1104 66-67 0.12378014926073888 1104 68-69 0.12040952378936964 1104 70-71 0.12611137519414228 1104 72-73 0.12887183025315352 1104 74-75 0.1763876549725012 1104 76-77 0.05090274594591193 1104 78-79 0.15077223989613486 1104 80-81 0.14482315157557224 1104 82-83 0.1783725815466255 1104 84-85 0.20013632823214067 1104 86-87 0.18482581094825434 1104 88-89 0.13268063656911266 1104 90-91 0.1516783184922872 1104 92-93 0.0826125949207821 1104 94-95 0.07737736206621548 1104 96-97 0.11809236961902769 1104 98-99 0.12116591246874009 1104 100 0.13650504112238337 1105 1 -0.0033683480192010506 1105 2 0.03436371871606525 1105 3 0.06439606332114778 1105 4 0.05970865496782096 1105 5 0.04279318053229275 1105 6 0.06739343617545046 1105 7 -0.03487489722164128 1105 8 -0.03023526811652033 1105 9 0.029909003126881828 1105 10-11 0.0076661291940709475 1105 12-13 0.036685082060394336 1105 14-15 0.06310271282202962 1105 16-17 0.05548024699306353 1105 18-19 0.050320140606906705 1105 20-21 0.08017088785081228 1105 22-23 0.08227373596081833 1105 24-25 0.033415134975960825 1105 26-27 0.03329457725065055 1105 28-29 0.05670674350245264 1105 30-31 0.05015014557410069 1105 32-33 0.05388919824603988 1105 34-35 0.08889484534694958 1105 36-37 0.02046786892078245 1105 38-39 0.0042358606549157685 1105 40-41 0.08026076905676405 1105 42-43 0.058800546304382806 1105 44-45 0.08733327629044396 1105 46-47 0.13868111311020215 1105 48-49 0.08465686446358944 1105 50-51 0.08324395458753742 1105 52-53 0.11064216211597255 1105 54-55 0.11049091676450473 1105 56-57 0.06770089976858884 1105 58-59 0.08273002529664097 1105 60-61 0.08094898939329198 1105 62-63 0.0590991199920623 1105 64-65 -0.00901631718943463 1105 66-67 0.02177932579493813 1105 68-69 0.08718545676124023 1105 70-71 0.08337002778583269 1105 72-73 0.08031115917813736 1105 74-75 0.053404526100109706 1105 76-77 0.02434458495338987 1105 78-79 0.08035926683735539 1105 80-81 0.078484250753597 1105 82-83 0.0898852286915286 1105 84-85 0.08471043271308076 1105 86-87 0.10112537391611198 1105 88-89 0.0827177917428088 1105 90-91 0.0809171086920486 1105 92-93 0.07039355301827754 1105 94-95 -0.004821986106037457 1105 96-97 -0.021761390792107704 1105 98-99 -0.05163550825779595 1105 100 -0.008103490181717632 1106 1 0.033939446534958506 1106 2 0.05357785715760599 1106 3 -8.05901379784757E-4 1106 4 0.007350432500267345 1106 5 -0.060725908361824565 1106 6 -0.017831101476957656 1106 7 0.007631938348950484 1106 8 0.055601700538161936 1106 9 0.02478036383109128 1106 10-11 0.04814284780410816 1106 12-13 0.010373746536743056 1106 14-15 0.020806405111954973 1106 16-17 0.03128493282773803 1106 18-19 0.06262354681223314 1106 20-21 0.038240736818238474 1106 22-23 0.06737156180442838 1106 24-25 0.044816996538934006 1106 26-27 0.06192655441957129 1106 28-29 0.02554950440131165 1106 30-31 0.08440455240761935 1106 32-33 0.055889476315904574 1106 34-35 0.0753995955817004 1106 36-37 0.014568216018950864 1106 38-39 0.05259403495881543 1106 40-41 0.06282330514422796 1106 42-43 0.10174550182919262 1106 44-45 0.052559962493496926 1106 46-47 0.06703026049117966 1106 48-49 0.033482357938822815 1106 50-51 -0.04939029078860813 1106 52-53 -0.0626188241365 1106 54-55 -0.0572790739282425 1106 56-57 -0.016392807894192174 1106 58-59 -0.017624880651588626 1106 60-61 -0.03170642541526547 1106 62-63 -0.06953750421720883 1106 64-65 -0.004501087220205591 1106 66-67 -0.019801356514754787 1106 68-69 0.019176792570860357 1106 70-71 0.011927821634458269 1106 72-73 0.09059161652968584 1106 74-75 0.030756507046866943 1106 76-77 -0.005986158266402697 1106 78-79 0.02215367537542079 1106 80-81 0.049867776261798724 1106 82-83 0.043507671902162315 1106 84-85 0.07883749558979503 1106 86-87 0.07183549247960741 1106 88-89 0.004578203305015904 1106 90-91 0.1034998810410741 1106 92-93 0.03892005177815605 1106 94-95 0.03729647353743104 1106 96-97 0.0684074096562668 1106 98-99 0.07882836232043644 1106 100 0.09675262935897422 1107 1 -0.04801092682554042 1107 2 -0.028312653854058567 1107 3 -0.061537848457312805 1107 4 0.0017565808041268838 1107 5 -0.0813619753732624 1107 6 -0.1309408125757443 1107 7 -0.07548103992291288 1107 8 -0.05653806855295329 1107 9 -0.08654254034185982 1107 10-11 -0.07833141031115787 1107 12-13 -0.045987263908180864 1107 14-15 -0.033975310469166686 1107 16-17 -0.009442672348725978 1107 18-19 -0.0022135566668737283 1107 20-21 -0.05862105746162172 1107 22-23 -0.0220907661137133 1107 24-25 -0.029869773989446458 1107 26-27 -0.040011466733943735 1107 28-29 -0.07129604357382391 1107 30-31 -0.017830022021591674 1107 32-33 -0.028365364617187083 1107 34-35 -0.010789057153623105 1107 36-37 -0.06550786755762772 1107 38-39 -0.05967493434839355 1107 40-41 -0.08218662534236643 1107 42-43 -0.0872776922337195 1107 44-45 -0.020527039602939112 1107 46-47 -0.1063343438441322 1107 48-49 -0.041945240502670345 1107 50-51 -0.0564338430655269 1107 52-53 -0.07398262170933378 1107 54-55 -0.07008128743800768 1107 56-57 -0.1155203258027413 1107 58-59 -0.11094598358043584 1107 60-61 -0.06976132538675017 1107 62-63 -0.04432655302561983 1107 64-65 -0.08816269621696193 1107 66-67 -0.14553645254527936 1107 68-69 -0.051612333617967465 1107 70-71 -0.00367099530497228 1107 72-73 -0.03438241087730631 1107 74-75 -0.047889274789085334 1107 76-77 -0.09500555495709762 1107 78-79 -0.06380355260017723 1107 80-81 -0.1066696507820808 1107 82-83 -0.01917861428213996 1107 84-85 -0.11332042923942964 1107 86-87 -0.07791486612129717 1107 88-89 -0.007730441377006514 1107 90-91 -0.06398042635979095 1107 92-93 -0.10327265841355882 1107 94-95 -0.1074852026369939 1107 96-97 -0.06410574501429522 1107 98-99 -0.08047887160198997 1107 100 -0.052472742532735595 1108 1 0.012870928976752793 1108 2 0.022849651133221016 1108 3 -0.05166141318307638 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10-11 -0.05964144988515585 2214 12-13 -0.09406065413296005 2214 14-15 -0.09002411315253767 2214 16-17 -0.1431234473591445 2214 18-19 -0.18507836387149723 2214 20-21 -0.1849232097729967 2214 22-23 -0.18722270565912424 2214 24-25 -0.16407390788540255 2214 26-27 -0.17533066812073628 2214 28-29 -0.13642788477432077 2214 30-31 -0.10189329320967744 2214 32-33 -0.13709137829106766 2214 34-35 -0.16793745439367314 2214 36-37 -0.2343479351140303 2214 38-39 -0.24493771853242663 2214 40-41 -0.15207491862527434 2214 42-43 -0.15706842735544768 2214 44-45 -0.1306639422886704 2214 46-47 -0.11537116024859273 2214 48-49 -0.23744386236252524 2214 50-51 -0.20072197007996095 2214 52-53 -0.18979887799357442 2214 54-55 -0.21956743522812872 2214 56-57 -0.22416461484764483 2214 58-59 -0.277281402700261 2214 60-61 -0.33484573155389796 2214 62-63 -0.3359224878399871 2214 64-65 -0.2766051028089578 2214 66-67 -0.35145206342459545 2214 68-69 -0.21742511504834283 2214 70-71 -0.13572553870916693 2214 72-73 -0.27516159112649774 2214 74-75 -0.19936002307591139 2214 76-77 -0.1485759345160389 2214 78-79 -0.3458958932355145 2214 80-81 -0.2313839915695226 2214 82-83 -0.29069599117396905 2214 84-85 -0.2842895331991926 2214 86-87 -0.2512427074371786 2214 88-89 -0.30250793271609666 2214 90-91 -0.26504124023612974 2214 92-93 -0.23737362848785892 2214 94-95 -0.320256306801312 2214 96-97 -0.3771895860259562 2214 98-99 -0.3921320562727715 2214 100 -0.5532396362231857 2215 1 -0.036209822780342904 2215 2 -0.09271666777794252 2215 3 -0.11825340298447884 2215 4 -0.058748694050237305 2215 5 -0.0442844150537951 2215 6 -0.05091031275500768 2215 7 -0.060924717598673794 2215 8 -0.06541886879501391 2215 9 -0.1011076985874908 2215 10-11 -0.11528828720071971 2215 12-13 -0.08495186555433065 2215 14-15 -0.11814674260038771 2215 16-17 -0.10146707729073512 2215 18-19 -0.09123257233252247 2215 20-21 -0.14031112130911083 2215 22-23 -0.12257521616341904 2215 24-25 -0.09911132773854803 2215 26-27 -0.038382097402603677 2215 28-29 -0.07770586886414321 2215 30-31 -0.06225754331043731 2215 32-33 -0.1244516871194179 2215 34-35 -0.04809136135850878 2215 36-37 0.02088436852802289 2215 38-39 -0.03409300310838148 2215 40-41 -0.07545905650481899 2215 42-43 -0.047463856833580564 2215 44-45 -0.053377056511969556 2215 46-47 -0.08970809569077431 2215 48-49 -0.0255702439988994 2215 50-51 -0.008467249205160954 2215 52-53 -0.036161413718104995 2215 54-55 -0.06907459629350399 2215 56-57 -0.1633242814985465 2215 58-59 -0.15377229606438192 2215 60-61 -0.11205449490167751 2215 62-63 -0.16835329853329029 2215 64-65 -0.012380462902875422 2215 66-67 -0.07164593278968567 2215 68-69 -0.022844385255346822 2215 70-71 -0.014967879720941824 2215 72-73 0.020460055450840287 2215 74-75 0.022877969242543372 2215 76-77 -0.04680522237047313 2215 78-79 -0.06002899318846033 2215 80-81 -0.08314181699402212 2215 82-83 -0.013293985988376278 2215 84-85 0.006957056832867181 2215 86-87 -0.04062266919340374 2215 88-89 -0.028038821390282465 2215 90-91 -0.050939369202183116 2215 92-93 -0.04295045876496317 2215 94-95 -0.025296099934351446 2215 96-97 0.006718455748021768 2215 98-99 -0.01668138964888044 2215 100 -0.03256503023541768 2216 1 -0.11887011787651147 2216 2 -0.1428200369800905 2216 3 -0.13185523097063623 2216 4 -0.11709139769858723 2216 5 -0.10140363948698194 2216 6 -0.09759354743967918 2216 7 -0.1323965387981545 2216 8 -0.08008835871374487 2216 9 -0.12088392348540111 2216 10-11 -0.18015082351238476 2216 12-13 -0.13921739248924325 2216 14-15 -0.226364826167611 2216 16-17 -0.24759684707541396 2216 18-19 -0.1907691642539433 2216 20-21 -0.19098164461052392 2216 22-23 -0.20071361835491075 2216 24-25 -0.28345816143069413 2216 26-27 -0.32032210946417194 2216 28-29 -0.32170776116816313 2216 30-31 -0.31391657134017237 2216 32-33 -0.2541478796941732 2216 34-35 -0.251565729626428 2216 36-37 -0.33274923932231104 2216 38-39 -0.2610984947044557 2216 40-41 -0.3417074442697796 2216 42-43 -0.3166497982846437 2216 44-45 -0.21655855465366614 2216 46-47 -0.2591478750528253 2216 48-49 -0.2544600111312292 2216 50-51 -0.1818642701226949 2216 52-53 -0.15154450517235318 2216 54-55 -0.11392587416676747 2216 56-57 -0.13734770420217046 2216 58-59 -0.14918046676161367 2216 60-61 -0.17351853564172615 2216 62-63 -0.12551063225746617 2216 64-65 -0.1634514346521101 2216 66-67 -0.15507357387013343 2216 68-69 -0.11540645950311301 2216 70-71 -0.1118440764361992 2216 72-73 -0.13114862793333515 2216 74-75 -0.11216328198402437 2216 76-77 -0.11962173743891213 2216 78-79 -0.09486793503472768 2216 80-81 -0.05792788951044514 2216 82-83 -0.10780293264498653 2216 84-85 -0.09312121175450727 2216 86-87 -0.14523227927000448 2216 88-89 -0.13289609124090163 2216 90-91 -0.11404213476351543 2216 92-93 -0.09564423053362248 2216 94-95 -0.1503932221035882 2216 96-97 -0.1315019348485933 2216 98-99 -0.1560487990399082 2216 100 -0.1763116617166034 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21.0 3 9.0 4 13.0 5 30.0 6 70.0 7 174.0 8 326.0 9 700.0 10 1330.0 11 2208.0 12 3335.0 13 5233.0 14 6433.0 15 8536.0 16 10421.0 17 15007.0 18 17463.0 19 9530.0 20 6293.0 21 5930.0 22 6176.0 23 6589.0 24 7312.0 25 8806.0 26 11150.0 27 14447.0 28 18595.0 29 23839.0 30 30971.0 31 39174.0 32 50994.0 33 66115.0 34 92121.0 35 140283.0 36 255434.0 37 608768.0 38 1249334.0 39 703988.0 40 3138.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.54084644305133 20.21256636316022 13.625080788348388 23.621506405440066 2 15.168304304794555 20.44235907347726 40.952876543803725 23.436460077924462 3 17.829166296193186 25.90258272247722 30.79875054477822 25.469500436551375 4 11.793751608828522 17.116332112931527 39.82023682538887 31.269679452851086 5 11.997199197320981 38.388931871728836 36.54327173064467 13.070597200305512 6 29.992510843825876 39.32629078623896 17.863605237220263 12.817593132714903 7 26.335001377484758 33.23464031613639 23.120695360135738 17.309662946243108 8 24.65019629587197 37.01991177982212 20.515024862394654 17.814867061911254 9 24.928606577640128 16.386554131897963 21.616668921093506 37.0681703693684 10-11 23.36181979048388 27.027491884785082 29.616781112939595 19.993907211791438 12-13 24.40537201454335 24.426055360820172 29.813039457819386 21.355533166817093 14-15 21.31923152325931 26.379106154941596 27.22629338460204 25.075368937197045 16-17 20.498668336493413 29.973317171462753 27.785561362634592 21.74245312940924 18-19 20.36041251887777 28.530388743930363 30.5185288189065 20.59066991828537 20-21 21.432640215305035 27.806055220890556 30.238163120616996 20.52314144318741 22-23 20.959090363152757 27.59499774363232 30.41364991668348 21.032261976531444 24-25 20.882874762338155 27.762212218544533 30.307999839080846 21.046913180036462 26-27 20.693546021985043 27.790904487468953 30.346001928144407 21.16954756240159 28-29 20.735636962577335 27.830358862196093 30.391267505317433 21.04273666990914 30-31 20.972235122709026 28.249767098621138 30.014641181688155 20.76335659698168 32-33 20.719180379017843 27.8750739927509 30.491384765220637 20.91436086301062 34-35 20.798282203007282 27.712759194652165 30.48019465333207 21.00876394900848 36-37 21.110958183901253 27.99348452326417 29.902069561986988 20.993487730847594 38-39 21.030192471098427 28.21464583579975 29.90801884179089 20.847142851310927 40-41 20.862234682552227 27.964836635081014 30.06280776182392 21.110120920542844 42-43 20.88006486124915 27.869517618515605 29.93340524911324 21.317012271122003 44-45 21.069284995317123 28.040706035487222 29.73670337155447 21.153305597641182 46-47 21.173174323685632 28.315633684177378 29.412571663825332 21.098620328311654 48-49 21.197251993459293 28.272667650075974 29.19674585691817 21.33333449954656 50-51 21.167581772822967 28.302946250183307 29.069559632433737 21.45991234455999 52-53 21.253486226589825 28.28828442740318 29.04789663175083 21.410332714256164 54-55 21.26378969276358 28.63680484504511 28.865978930619086 21.23342653157222 56-57 21.09646587920101 28.375263559512888 28.94206683133139 21.58620372995472 58-59 21.0142495008761 28.021807673207327 29.304581969795006 21.659360856121566 60-61 21.17864705233289 28.372883791864833 29.02402587776714 21.424443278035135 62-63 21.08259408967535 28.370368761590804 28.99469616149137 21.55234098724248 64-65 21.072753149814503 28.084322730847923 29.135791419863903 21.707132699473675 66-67 20.95153873209309 28.229088305006407 29.01331102997412 21.80606193292638 68-69 21.366726905037257 28.320250266670673 28.931428249920344 21.381594578371732 70-71 21.27967067421007 28.149588911229138 28.95510601026563 21.615634404295157 72-73 21.168445508239788 28.32845350176684 28.966740426534255 21.536360563459116 74-75 21.154694089528935 28.407243556022376 28.822224680721803 21.615837673726883 76-77 21.23142300046046 28.348706455311927 28.699712429016433 21.720158115211177 78-79 21.25550426421051 28.447130640367664 28.78192691882185 21.51543817659997 80-81 21.282322583541564 28.23743285358193 28.8934136201862 21.586830942690305 82-83 21.079443836299316 28.34611409839124 29.015417086335393 21.55902497897405 84-85 21.0469384854768 28.279451795221245 28.81493169304322 21.858678026258733 86-87 21.121223313837405 28.406950551396694 28.726369702377845 21.745456432388057 88-89 20.993800534997565 28.215393073950466 28.815924340056952 21.974882050995014 90-91 20.939096800975776 28.20619561693801 28.85221858405222 22.00248899803399 92-93 21.022777626187363 28.383381492442634 28.71402351283971 21.879817368530297 94-95 20.86561917688736 28.119818231429587 28.84452245520503 22.17004013647802 96-97 20.86337447264026 28.1523810190141 28.827220741300458 22.157023767045175 98-99 20.787550112293516 28.241629876838616 28.720772201582605 22.250047809285263 100 20.966149859953774 28.109789942325676 28.56074227996651 22.363317917754035 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 35.0 1 30.5 2 112.5 3 212.0 4 158.0 5 134.5 6 311.0 7 726.5 8 1302.0 9 1558.0 10 1331.0 11 1142.0 12 1213.5 13 1431.0 14 1699.0 15 1930.0 16 2199.0 17 2592.5 18 3073.0 19 3604.5 20 4382.0 21 5620.5 22 7541.0 23 10444.5 24 14440.5 25 19491.5 26 26481.5 27 35227.5 28 45964.5 29 56660.0 30 65969.5 31 76216.5 32 86806.0 33 97380.5 34 106750.5 35 113954.5 36 119208.0 37 123847.5 38 126928.5 39 127464.5 40 127751.0 41 127389.0 42 126372.5 43 125056.5 44 121637.5 45 117588.5 46 113920.0 47 108756.0 48 104403.0 49 101173.5 50 97424.5 51 93215.0 52 86597.0 53 81757.5 54 78928.0 55 74474.0 56 68695.5 57 62429.0 58 55911.0 59 49048.0 60 42395.5 61 35356.0 62 29459.0 63 24280.5 64 19385.0 65 15194.5 66 11800.5 67 8925.5 68 6655.5 69 5336.0 70 4019.0 71 2741.0 72 2009.5 73 1520.5 74 1176.5 75 816.0 76 464.0 77 285.5 78 176.5 79 102.0 80 67.5 81 28.0 82 8.5 83 4.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 1.5 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09127492204754342 2 0.0014867521636616782 3 2.915200320905251E-5 4 1.4576001604526254E-4 5 0.03728541210437816 6 8.745600962715755E-5 7 0.003818912420385879 8 4.372800481357877E-4 9 2.332160256724201E-4 10-11 3.2067203529957764E-4 12-13 0.0 14-15 1.0203201123168378E-4 16-17 0.0 18-19 1.3118401444073632E-4 20-21 0.0 22-23 1.1660801283621005E-4 24-25 5.830400641810502E-5 26-27 0.0016033601764978882 28-29 0.008818480970738384 30-31 0.037008468073892166 32-33 0.02641171490740158 34-35 0.029735043273233563 36-37 0.02690729896195547 38-39 0.026178498881729153 40-41 0.03212550753637587 42-43 0.02394837063623664 44-45 0.02273856250306096 46-47 0.016529185819532777 48-49 0.011413009256344059 50-51 0.00801680088248944 52-53 0.005553456611324504 54-55 0.004445680489380509 56-57 0.00272571230004641 58-59 0.0010348961139213643 60-61 4.5185604974031396E-4 62-63 0.0012972641428028368 64-65 0.002740288301650936 66-67 5.976160657855766E-4 68-69 0.0010203201123168378 70-71 4.372800481357877E-4 72-73 5.976160657855766E-4 74-75 5.68464062576524E-4 76-77 3.644000401131564E-4 78-79 2.915200320905251E-5 80-81 3.644000401131564E-4 82-83 3.2067203529957764E-4 84-85 2.4779202727694637E-4 86-87 2.0406402246336757E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3430296.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.10375090203627 #Duplication Level Percentage of deduplicated Percentage of total 1 74.75517736289419 46.42576913582722 2 14.981698946153713 18.608393988824588 3 4.953550612946605 9.22902220041195 4 2.0849879820622617 5.179422970869358 5 1.0490465218015363 3.257486193730509 6 0.594159303255959 2.2139712819321304 7 0.36315291976024555 1.5787210927696218 8 0.24063972771166198 1.1955703765551111 9 0.16714004462699905 0.9342021327543337 >10 0.7639694286327382 8.077077031948269 >50 0.0339505877956445 1.4368542711185712 >100 0.01205666535297308 1.3199585655226564 >500 2.8193820424401403E-4 0.10782884480341082 >1k 1.4096910085362768E-4 0.26191173856857347 >5k 4.698970028454256E-5 0.1738101743637241 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5956 0.1736293311131168 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4958 0.14453563191048235 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.915200320905251E-5 0.0 5 0.0 2.915200320905251E-5 0.0 2.915200320905251E-5 0.0 6 0.0 2.915200320905251E-5 0.0 2.915200320905251E-5 0.0 7 0.0 2.915200320905251E-5 0.0 2.915200320905251E-5 0.0 8 0.0 2.915200320905251E-5 0.0 5.830400641810502E-5 0.0 9 0.0 2.915200320905251E-5 0.0 8.745600962715753E-5 0.0 10-11 0.0 2.915200320905251E-5 0.0 8.745600962715753E-5 0.0 12-13 0.0 2.915200320905251E-5 0.0 8.745600962715753E-5 0.0 14-15 0.0 2.915200320905251E-5 0.0 2.3321602567242012E-4 0.0 16-17 0.0 2.915200320905251E-5 0.0 2.769440304859989E-4 0.0 18-19 0.0 2.915200320905251E-5 0.0 3.498240385086302E-4 0.0 20-21 0.0 2.915200320905251E-5 0.0 4.664320513448402E-4 0.0 22-23 0.0 2.915200320905251E-5 0.0 5.830400641810502E-4 0.0 24-25 0.0 2.915200320905251E-5 0.0 7.433760818308391E-4 0.0 26-27 0.0 2.915200320905251E-5 0.0 9.474401042942066E-4 0.0 28-29 0.0 2.915200320905251E-5 0.0 0.0015159041668707306 0.0 30-31 0.0 2.915200320905251E-5 0.0 0.005159904568002294 0.0 32-33 0.0 2.915200320905251E-5 0.0 0.01339534547455963 0.0 34-35 0.0 2.915200320905251E-5 0.0 0.023933794634632112 0.0 36-37 0.0 2.915200320905251E-5 0.0 0.04231413265793972 0.0 38-39 0.0 2.915200320905251E-5 0.0 0.07506640826331022 0.0 40-41 0.0 2.915200320905251E-5 0.0 0.11555854072068417 0.0 42-43 0.0 2.915200320905251E-5 0.0 0.15657540923582103 0.0 44-45 0.0 2.915200320905251E-5 0.0 0.20231490227082444 0.0 46-47 0.0 2.915200320905251E-5 0.0 0.26058975668572043 0.0 48-49 0.0 2.915200320905251E-5 0.0 0.32673565196706056 0.0 50-51 0.0 2.915200320905251E-5 0.0 0.39961565998969184 0.0 52-53 0.0 2.915200320905251E-5 0.0 0.4709068838374298 0.0 54-55 0.0 2.915200320905251E-5 0.0 0.5495589884954535 0.0 56-57 0.0 2.915200320905251E-5 0.0 0.6456148390692815 0.0 58-59 0.0 2.915200320905251E-5 0.0 0.7544975710550925 0.0 60-61 0.0 2.915200320905251E-5 0.0 0.8716448959506702 0.0 62-63 0.0 2.915200320905251E-5 0.0 0.9860956605494103 0.0 64-65 0.0 2.915200320905251E-5 0.0 1.1091171140916118 0.0 66-67 0.0 5.830400641810502E-5 0.0 1.2507375456811891 0.0 68-69 0.0 5.830400641810502E-5 0.0 1.4083186990277223 0.0 70-71 0.0 5.830400641810502E-5 0.0 1.5823853101889749 0.0 72-73 0.0 5.830400641810502E-5 0.0 1.7674568025616448 0.0 74-75 0.0 5.830400641810502E-5 0.0 1.9532133670097276 0.0 76-77 0.0 5.830400641810502E-5 0.0 2.1516219008505386 0.0 78-79 0.0 5.830400641810502E-5 0.0 2.3833949023641106 0.0 80-81 0.0 5.830400641810502E-5 0.0 2.6394515225508233 0.0 82-83 0.0 5.830400641810502E-5 0.0 2.9058279518735413 0.0 84-85 0.0 5.830400641810502E-5 0.0 3.171169485082337 0.0 86-87 0.0 5.830400641810502E-5 0.0 3.450358219815433 0.0 88 0.0 5.830400641810502E-5 0.0 3.6805278611525067 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4745 0.0 52.062332 1 GTATCAA 8530 0.0 42.641357 1 ATCAACG 10020 0.0 35.51904 3 TCAACGC 10140 0.0 35.145065 4 CAACGCA 10345 0.0 34.448116 5 AACGCAG 10770 0.0 33.24977 6 TATCAAC 10775 0.0 33.117985 2 ACGCAGA 12265 0.0 28.813747 7 CGCAGAG 12215 0.0 28.776539 8 GTACATG 8840 0.0 26.188667 1 GCAGAGT 13870 0.0 25.173452 9 TACATGG 8905 0.0 25.131155 2 ACATGGG 8980 0.0 23.612028 3 GAGTACT 9720 0.0 21.417458 12-13 AGAGTAC 13040 0.0 20.66742 10-11 CAGAGTA 13920 0.0 20.559309 10-11 CATGGGG 5730 0.0 19.774044 4 GTGGTAT 2350 0.0 19.622702 1 GTATAGA 1390 0.0 18.957182 1 AGTACTT 10645 0.0 18.87213 12-13 >>END_MODULE