FastQCFastQC Report
Wed 25 May 2016
SRR1033171_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033171_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3703012
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47000.035.982151
TCAACGC47100.034.505984
ATCAACG49100.033.105363
CAACGCA50150.032.4069675
GGTATCA30950.031.2020951
GTACATG111750.030.3511751
AACGCAG54350.029.9813886
TACATGG112000.028.746672
ACATGGG110150.027.9357593
TATCAAC61200.027.0337452
ACGCAGA61800.025.4556397
CGCAGAG60850.025.3128438
CATGGGG74500.021.752144
GCAGAGT77600.018.8202929
GAGTACA72450.017.9457361
TATACTG20750.017.656715
CATGGGA42350.017.080734
AGAGTAC65100.016.80748210-11
AGTACAT76350.016.2521132
CTATACT19750.016.1726424
Produced by FastQC (version 0.11.5)