##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033171_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3703012
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.650329785590756	33.0	31.0	34.0	30.0	34.0
2	31.736456160552546	34.0	31.0	34.0	30.0	34.0
3	31.887752456648805	34.0	31.0	34.0	30.0	34.0
4	35.26214173759091	37.0	35.0	37.0	33.0	37.0
5	35.34834696727961	37.0	35.0	37.0	33.0	37.0
6	35.48070759695081	37.0	37.0	37.0	35.0	37.0
7	35.384215606106594	37.0	36.0	37.0	33.0	37.0
8	35.398651152089165	37.0	36.0	37.0	33.0	37.0
9	37.074652472095686	39.0	38.0	39.0	35.0	39.0
10-11	37.0598555986316	39.0	38.0	39.0	34.5	39.0
12-13	37.02188556234762	39.0	38.0	39.0	34.5	39.0
14-15	38.47887044384409	41.0	39.0	41.0	35.0	41.0
16-17	38.482701919410474	41.0	39.0	41.0	34.5	41.0
18-19	38.4620742519873	41.0	39.0	41.0	34.0	41.0
20-21	38.49729828042685	41.0	39.0	41.0	34.0	41.0
22-23	38.45811220703578	41.0	39.0	41.0	34.0	41.0
24-25	38.44997099658332	41.0	39.0	41.0	34.0	41.0
26-27	38.40959265052341	40.5	39.0	41.0	34.0	41.0
28-29	38.37891519120111	40.0	39.0	41.0	34.0	41.0
30-31	38.3713875353361	40.0	39.0	41.0	34.0	41.0
32-33	38.446691909180956	41.0	39.0	41.0	34.0	41.0
34-35	38.42024073916045	41.0	39.0	41.0	34.0	41.0
36-37	38.342464188611864	41.0	39.0	41.0	34.0	41.0
38-39	38.264214239651395	41.0	39.0	41.0	33.5	41.0
40-41	38.20273293740339	41.0	38.0	41.0	33.0	41.0
42-43	38.08831000277611	40.5	38.0	41.0	33.0	41.0
44-45	37.95065435920812	40.0	38.0	41.0	33.0	41.0
46-47	37.82217637966067	40.0	38.0	41.0	33.0	41.0
48-49	37.70425831728333	40.0	38.0	41.0	33.0	41.0
50-51	37.575729163178515	40.0	37.5	41.0	32.0	41.0
52-53	37.42894068396214	40.0	37.0	41.0	32.0	41.0
54-55	37.27575038914267	40.0	37.0	41.0	32.0	41.0
56-57	37.11140188041519	40.0	36.5	41.0	31.0	41.0
58-59	36.912141521550566	40.0	36.0	41.0	31.0	41.0
60-61	36.68780373922634	39.0	35.5	41.0	31.0	41.0
62-63	36.42582997300576	39.0	35.0	41.0	31.0	41.0
64-65	36.15348383964189	39.0	35.0	41.0	30.5	41.0
66-67	35.85771353157916	38.0	35.0	40.0	30.0	41.0
68-69	35.54979014380726	37.0	35.0	40.0	30.0	41.0
70-71	35.19964923149047	37.0	35.0	40.0	30.0	41.0
72-73	34.83002242498809	36.0	34.0	39.0	29.0	41.0
74-75	34.45383379800011	36.0	34.0	39.0	29.0	41.0
76-77	34.12159277366641	35.5	34.0	38.5	29.0	40.0
78-79	33.750748444779546	35.0	34.0	37.0	29.0	39.0
80-81	33.39148077294915	35.0	34.0	37.0	28.0	39.0
82-83	33.173303246114244	35.0	34.0	36.5	28.5	39.0
84-85	32.905590638107576	35.0	34.0	36.0	28.0	37.0
86-87	32.64992241451013	35.0	34.0	36.0	28.0	37.0
88-89	32.29330879835118	35.0	34.0	35.0	27.0	37.0
90-91	32.10296901549333	35.0	34.0	35.0	27.0	36.0
92-93	31.926177527915115	35.0	33.5	35.0	26.0	36.0
94-95	31.75769913789099	35.0	33.0	35.0	26.0	36.0
96-97	31.582825278449004	35.0	33.0	35.0	25.5	36.0
98-99	31.389784316118877	35.0	33.0	35.0	25.0	35.0
100	31.2302161051598	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.08718949219333894
1101	2	0.11520477017763397
1101	3	0.08535963044975148
1101	4	0.041170657022242096
1101	5	0.10784620130102951
1101	6	0.043137728508582995
1101	7	0.04023824151877875
1101	8	0.022013961333207988
1101	9	0.110873707913548
1101	10-11	0.07025394776314187
1101	12-13	0.06808976917945841
1101	14-15	0.04729851912674832
1101	16-17	0.02799292937363873
1101	18-19	-0.008068731776717186
1101	20-21	-0.010485200908547654
1101	22-23	0.03382141216835066
1101	24-25	0.03487552424434881
1101	26-27	0.03200625018988745
1101	28-29	0.06502757435131201
1101	30-31	0.053668080803980445
1101	32-33	0.1090090834562929
1101	34-35	0.10900617438655047
1101	36-37	0.12023780343179169
1101	38-39	0.09883337499660882
1101	40-41	0.06124822044311884
1101	42-43	0.014624972749651022
1101	44-45	-0.011945098886378958
1101	46-47	0.008104544759277132
1101	48-49	-0.021559855240923298
1101	50-51	0.035580645636876795
1101	52-53	5.1600122696982E-4
1101	54-55	-0.06421674558790613
1101	56-57	-0.024122105611262157
1101	58-59	0.0021843736096585076
1101	60-61	0.0048771290812510415
1101	62-63	0.015495930969578353
1101	64-65	-0.007363266287221393
1101	66-67	0.009793278505995318
1101	68-69	0.050579905743695974
1101	70-71	-0.0015988093221253052
1101	72-73	0.05801047248127844
1101	74-75	0.040049762195351946
1101	76-77	-0.029585145273500757
1101	78-79	-0.005599535269276146
1101	80-81	-0.013304680442438155
1101	82-83	-0.008916494364150651
1101	84-85	-0.11406856416144961
1101	86-87	0.0032800481349752886
1101	88-89	0.0636113732932273
1101	90-91	0.136897699822498
1101	92-93	0.11960856666767228
1101	94-95	0.16838547107515822
1101	96-97	0.1759495204338748
1101	98-99	0.22214889460670406
1101	100	0.26229352141157136
1102	1	0.1793202535376679
1102	2	0.2185703123827274
1102	3	0.2362107525192343
1102	4	0.24500484649659882
1102	5	0.12205546467965434
1102	6	0.12814547013508104
1102	7	0.09186771398794491
1102	8	0.10944998869672418
1102	9	0.19192246512265854
1102	10-11	0.1742421131823022
1102	12-13	0.1722529444683829
1102	14-15	0.22338207082585626
1102	16-17	0.2592696905222809
1102	18-19	0.2259624837100418
1102	20-21	0.23879945529051838
1102	22-23	0.2523449334263219
1102	24-25	0.25743786365841714
1102	26-27	0.26003284163949303
1102	28-29	0.24898599913304764
1102	30-31	0.2600052502430046
1102	32-33	0.2873825919035511
1102	34-35	0.2904349318164705
1102	36-37	0.24563974175080716
1102	38-39	0.16406728556682992
1102	40-41	0.23314124323323426
1102	42-43	0.2560631183531399
1102	44-45	0.19823842353654442
1102	46-47	0.16709436814664258
1102	48-49	0.16160651377825985
1102	50-51	0.1734224088470384
1102	52-53	0.07058185168851594
1102	54-55	0.21851215586253403
1102	56-57	0.1508634752048792
1102	58-59	0.1460647197005258
1102	60-61	0.12802731435111525
1102	62-63	0.12858422891198273
1102	64-65	0.166378229249581
1102	66-67	0.1458815166777896
1102	68-69	0.06442774728228784
1102	70-71	0.12963563651866394
1102	72-73	0.17908391457174133
1102	74-75	0.18342512277012446
1102	76-77	0.15086739058030219
1102	78-79	0.09109954711860979
1102	80-81	0.13949665765782981
1102	82-83	0.14372924979708301
1102	84-85	0.13096114792314495
1102	86-87	0.16344207978751513
1102	88-89	0.24438541411770132
1102	90-91	0.25929507996625034
1102	92-93	0.3135434677168547
1102	94-95	0.2672226852469599
1102	96-97	0.29250948196748894
1102	98-99	0.3846182240984781
1102	100	0.33693071951764253
1103	1	0.16430493042759053
1103	2	0.1599028930535269
1103	3	0.15022609918067786
1103	4	0.19397918433511308
1103	5	0.11912721981605756
1103	6	0.12678824884346795
1103	7	0.12325087122572143
1103	8	0.08651551743970032
1103	9	0.1127549766113134
1103	10-11	0.1602213938645889
1103	12-13	0.14295695379473017
1103	14-15	0.20776493661542617
1103	16-17	0.2048725025558582
1103	18-19	0.17803077237918075
1103	20-21	0.22926747988992702
1103	22-23	0.24256537424908942
1103	24-25	0.25298786910467896
1103	26-27	0.2942019051487037
1103	28-29	0.3418649480354006
1103	30-31	0.33521861093517913
1103	32-33	0.30069474617680214
1103	34-35	0.3124928137240133
1103	36-37	0.332066756055319
1103	38-39	0.31091232196841645
1103	40-41	0.3184275984526579
1103	42-43	0.24793704934173633
1103	44-45	0.23778371282097055
1103	46-47	0.2385658945403648
1103	48-49	0.2502441921430858
1103	50-51	0.20355631310483346
1103	52-53	0.16063643934818828
1103	54-55	0.19527258396756508
1103	56-57	0.16643001076892716
1103	58-59	0.16209164774814866
1103	60-61	0.138604418592422
1103	62-63	0.19842095903977963
1103	64-65	0.2162891756319496
1103	66-67	0.15444957866499465
1103	68-69	0.1414371207275309
1103	70-71	0.1058775664945486
1103	72-73	0.1963411809053568
1103	74-75	0.16191164549866954
1103	76-77	0.11098910700414422
1103	78-79	0.14503016036683647
1103	80-81	0.20856200682160164
1103	82-83	0.17242718286997416
1103	84-85	0.14804457570728147
1103	86-87	0.16113452391925165
1103	88-89	0.18003696276746695
1103	90-91	0.13875758618527811
1103	92-93	0.14222325887508447
1103	94-95	0.20148466627044215
1103	96-97	0.19589462340818287
1103	98-99	0.2300483015530439
1103	100	0.2836848991498293
1104	1	0.1388982361846054
1104	2	0.14065129937096188
1104	3	0.10879010545238543
1104	4	0.16436590197704248
1104	5	0.0422272199571907
1104	6	0.12983353384344554
1104	7	0.047746622581598785
1104	8	0.08350082183060437
1104	9	0.15450268575877857
1104	10-11	0.1901073345994888
1104	12-13	0.24387414157477139
1104	14-15	0.21109995642267876
1104	16-17	0.20315025281376364
1104	18-19	0.1906213056859869
1104	20-21	0.20197877954741017
1104	22-23	0.18704700579616684
1104	24-25	0.15659972819166512
1104	26-27	0.20230749393979153
1104	28-29	0.25852568734605796
1104	30-31	0.24980385831055685
1104	32-33	0.2675289135737984
1104	34-35	0.22556046390840123
1104	36-37	0.23485248989727836
1104	38-39	0.19875522657044797
1104	40-41	0.19205852033998383
1104	42-43	0.2142827038135806
1104	44-45	0.20537488825799954
1104	46-47	0.13287639132337148
1104	48-49	0.09561533283452661
1104	50-51	0.1469201793780357
1104	52-53	0.07166059826342774
1104	54-55	0.06999458038574602
1104	56-57	0.13241448462434136
1104	58-59	0.12919909095490567
1104	60-61	0.11863986629832368
1104	62-63	0.1277869154116189
1104	64-65	0.039584884367322104
1104	66-67	0.05636661967241707
1104	68-69	0.03171989089148042
1104	70-71	0.10133821731824355
1104	72-73	0.11139652406398426
1104	74-75	0.04565772391759282
1104	76-77	0.04769718754279495
1104	78-79	0.03715995464524724
1104	80-81	0.0842449229114095
1104	82-83	0.10833080123857286
1104	84-85	0.09658369414009371
1104	86-87	0.18297596644013225
1104	88-89	0.19903763171193134
1104	90-91	0.16041676250860348
1104	92-93	0.15659965515552443
1104	94-95	0.20697436819500226
1104	96-97	0.2817749910171621
1104	98-99	0.345970202992401
1104	100	0.3762519947030398
1105	1	0.11872247827956528
1105	2	0.12198401728557329
1105	3	0.12066250161568348
1105	4	0.15017107485815728
1105	5	0.15212305578581464
1105	6	0.14393600738021917
1105	7	0.0770936552334831
1105	8	0.047686286171874315
1105	9	0.042907788664557245
1105	10-11	0.03943919312970934
1105	12-13	0.11211352722433787
1105	14-15	0.07492721594497453
1105	16-17	0.08601714789075032
1105	18-19	0.13010301928596846
1105	20-21	0.1455767854032004
1105	22-23	0.19113462019203808
1105	24-25	0.20909577402785118
1105	26-27	0.20301616667168787
1105	28-29	0.24602273503852246
1105	30-31	0.21737655507151032
1105	32-33	0.2398498008858212
1105	34-35	0.23100529879986453
1105	36-37	0.20993441972289162
1105	38-39	0.17974331747749517
1105	40-41	0.15045671439592923
1105	42-43	0.18324816258848386
1105	44-45	0.12955692960448317
1105	46-47	0.12598588988249304
1105	48-49	0.1644166788183341
1105	50-51	0.15030207717210686
1105	52-53	0.1580434431404356
1105	54-55	0.07398290958406761
1105	56-57	0.11443057827236913
1105	58-59	0.03667299996365614
1105	60-61	0.023803644571032123
1105	62-63	0.05607692427967237
1105	64-65	0.04138931298322035
1105	66-67	0.10683859982326283
1105	68-69	0.037292977657735094
1105	70-71	0.042811886914435604
1105	72-73	0.08367035114375909
1105	74-75	0.11046975532637049
1105	76-77	0.13155121601250386
1105	78-79	0.16464296377775156
1105	80-81	0.11220598925073944
1105	82-83	0.10131016211577304
1105	84-85	0.10407126590651927
1105	86-87	0.1869387268119027
1105	88-89	0.20142149580823343
1105	90-91	0.19681780117718972
1105	92-93	0.12328002535407734
1105	94-95	0.1868635432168979
1105	96-97	0.2617526970391246
1105	98-99	0.34282705517798107
1105	100	0.31471576320013384
1106	1	0.17328204325922414
1106	2	0.15331187118598422
1106	3	0.12952211652788748
1106	4	0.16621243360608418
1106	5	0.15584202408216896
1106	6	0.13188552100839956
1106	7	0.10131688170745434
1106	8	0.16557685124433164
1106	9	0.11323657918892138
1106	10-11	0.11520871653491582
1106	12-13	0.12792157282907368
1106	14-15	0.1687988017395199
1106	16-17	0.11305022988111091
1106	18-19	0.15799285934634355
1106	20-21	0.14329139348016184
1106	22-23	0.14571567367877236
1106	24-25	0.20154620521365274
1106	26-27	0.16065184499086627
1106	28-29	0.19038853194708594
1106	30-31	0.1464589499942619
1106	32-33	0.16598715745249137
1106	34-35	0.21544148243446415
1106	36-37	0.2032333932661743
1106	38-39	0.1897368480130197
1106	40-41	0.20066149348572537
1106	42-43	0.22204683229769273
1106	44-45	0.13479799775839751
1106	46-47	0.14797439344936691
1106	48-49	0.20637731769963352
1106	50-51	0.19346729989729994
1106	52-53	0.14158416320672274
1106	54-55	0.10620508143549756
1106	56-57	0.10027295802715486
1106	58-59	0.11091381166296088
1106	60-61	0.1150178728758604
1106	62-63	0.11599452493810247
1106	64-65	0.19119044200541424
1106	66-67	0.15679692390301625
1106	68-69	0.15608861311773126
1106	70-71	0.10072599933189252
1106	72-73	0.13920279068830865
1106	74-75	0.13920350617719635
1106	76-77	0.15937045331615707
1106	78-79	0.14896327640688867
1106	80-81	0.14607177822182393
1106	82-83	0.053716735798680304
1106	84-85	0.0824942844345884
1106	86-87	0.13482728050772153
1106	88-89	0.26615664627605895
1106	90-91	0.22675669231458073
1106	92-93	0.25256930239265074
1106	94-95	0.21788744848548092
1106	96-97	0.18266040889281143
1106	98-99	0.19751191795675993
1106	100	0.23644434257087354
1107	1	0.16531724552021743
1107	2	0.16726882263790444
1107	3	0.1936894877214499
1107	4	0.13472557064774549
1107	5	0.09258079489308102
1107	6	0.13149918066670807
1107	7	0.07276200789831222
1107	8	0.1410500884492265
1107	9	0.1404663481635282
1107	10-11	0.13325018806874311
1107	12-13	0.11482542656772665
1107	14-15	0.047753527793496175
1107	16-17	0.06778820061220614
1107	18-19	0.14847552204201264
1107	20-21	0.11131192469187567
1107	22-23	0.12605408742513902
1107	24-25	0.12552991148489667
1107	26-27	0.12022782770814189
1107	28-29	0.14160633913410692
1107	30-31	0.11176912480139123
1107	32-33	0.15615573095520574
1107	34-35	0.15990870215083675
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2216	8	-0.035948466948752866
2216	9	-0.1127957322218407
2216	10-11	-0.08962306460984593
2216	12-13	-0.08137582471643867
2216	14-15	-0.1372017740543825
2216	16-17	-0.09038893347697297
2216	18-19	-0.14842463139743955
2216	20-21	-0.1600259827743642
2216	22-23	-0.12789321497205464
2216	24-25	-0.1608342338626656
2216	26-27	-0.2062044542612682
2216	28-29	-0.1888283883590205
2216	30-31	-0.17966401547818833
2216	32-33	-0.12047224573043991
2216	34-35	-0.13403749104553953
2216	36-37	-0.14466730237319325
2216	38-39	-0.12196964439787195
2216	40-41	-0.12972053906671022
2216	42-43	-0.13535988574406588
2216	44-45	-0.12690735645527695
2216	46-47	0.014941231738326621
2216	48-49	-0.015676860488490263
2216	50-51	0.007282135293891656
2216	52-53	0.0026485784746270724
2216	54-55	0.021650216981392134
2216	56-57	-0.06273302085593713
2216	58-59	-0.08402067504356836
2216	60-61	-0.07244950438295916
2216	62-63	0.014823989516735026
2216	64-65	0.040933611148226134
2216	66-67	0.06828387815417614
2216	68-69	-0.053782703642554
2216	70-71	-0.07066708712422809
2216	72-73	-0.005536796480861028
2216	74-75	0.03337881533165188
2216	76-77	-0.012646030457830193
2216	78-79	0.002729035417054604
2216	80-81	0.01317573580054443
2216	82-83	0.009076439599589037
2216	84-85	-0.010163540001840943
2216	86-87	-0.05619615965992608
2216	88-89	-0.08576255511120223
2216	90-91	-0.1159082291163287
2216	92-93	-0.04685195779254059
2216	94-95	-0.06579321354359635
2216	96-97	-0.07458147140901161
2216	98-99	-0.10342555484367111
2216	100	-0.07011984170995333
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	36303.0
3	6025.0
4	4076.0
5	3391.0
6	2922.0
7	3327.0
8	3826.0
9	4292.0
10	4674.0
11	5148.0
12	5665.0
13	6248.0
14	6867.0
15	7103.0
16	7250.0
17	7488.0
18	7549.0
19	7289.0
20	7430.0
21	7811.0
22	9118.0
23	11928.0
24	17995.0
25	24930.0
26	27463.0
27	24128.0
28	25185.0
29	32309.0
30	39764.0
31	48398.0
32	63492.0
33	87108.0
34	122676.0
35	188499.0
36	355394.0
37	760498.0
38	1209688.0
39	508772.0
40	983.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.16973617367155	19.59754800690893	13.169397500592678	24.063318318826838
2	15.199971449258356	20.21333027638524	40.59363913268471	23.9930591416717
3	18.017698617640033	25.76057361074931	30.027822304754757	26.193905466855895
4	11.963083862319298	16.77654319953728	39.27748131270466	31.982891625438764
5	12.694301078057594	38.410124404561216	35.914333540818355	12.98124097656284
6	30.7005148747599	38.5356642057252	18.044302347941258	12.71951857157364
7	27.230967336310208	32.660962102385085	22.846947390140233	17.261123171164474
8	25.19584999234291	36.79349166268507	20.385225894954452	17.62543245001757
9	25.294765088707056	16.717359626507992	20.824853370354816	37.16302191443014
10-11	23.827049850280073	26.992236604482795	29.241074201984418	19.93963934325272
12-13	24.472764614336654	24.31345015403414	29.08213425467518	22.131650976954027
14-15	21.714138548352768	26.245134147883007	25.995318626206405	26.04540867755782
16-17	21.74884709884327	29.55566290651693	26.57781716480649	22.117672829833314
18-19	21.735277959196242	28.62795511770232	28.257159186733887	21.37960773636755
20-21	22.753342465005986	27.991701000477292	27.747409398851957	21.507547135664765
22-23	22.280509539035627	27.84264558185815	27.758268895050282	22.118575984055937
24-25	22.227860177918394	27.904463707734863	27.70126770266691	22.166408411679832
26-27	22.030174343502416	27.980960574018845	27.695207123646203	22.29365795883254
28-29	22.119976733441653	27.959899084611916	27.81634004396445	22.10378413798198
30-31	22.23855301962266	28.337072700289323	27.461786676869398	21.962587603218616
32-33	21.9464967375203	27.972819151982176	27.948103396674096	22.132580713823426
34-35	22.058681333758997	27.962682967277235	27.874092692436715	22.10454300652705
36-37	22.391410470480917	28.022097701712084	27.476407084390292	22.110084743416703
38-39	22.158454669940124	28.20273274076235	27.60035759525295	22.03845499404458
40-41	21.950853261309174	27.98923688772342	27.921501553010796	22.13840829795661
42-43	21.958819448917538	27.953413385846442	27.839752915073063	22.248014250162957
44-45	22.0177928624103	28.035688893768256	27.86732365300718	22.079194590814264
46-47	22.090842260142857	28.15582882131545	27.761184799000947	21.992144119540747
48-49	22.130918763056652	28.004753631219813	27.64012942991128	22.224198175812248
50-51	22.02400050124826	27.979024272730907	27.71346532150647	22.283509904514368
52-53	22.11258963340743	27.944233443459552	27.718858644764214	22.2243182783688
54-55	22.17113741613294	28.227497809815265	27.613152342876525	21.988212431175267
56-57	21.944666642541442	28.020752372058453	27.689093581196584	22.345487404203514
58-59	21.896900100299927	27.74324622849047	27.926589310955162	22.43326436025444
60-61	22.144638100928674	28.017783539086317	27.64131803435855	22.196260325626458
62-63	22.00538714210059	28.01235575122249	27.652636818096994	22.329620288579925
64-65	22.038725696181064	27.856924110619847	27.700979444308217	22.403370748890875
66-67	21.8419536054884	27.92185556441939	27.702543223786087	22.53364760630613
68-69	22.14314707185791	27.998053945621205	27.61495993520714	22.243839047313752
70-71	22.09943886002044	27.770615990106158	27.74270549758386	22.387239652289544
72-73	21.958198175523158	27.93026160808842	27.775492046894286	22.336048169494138
74-75	21.96311257980367	28.019786030764138	27.622977083868104	22.39412430556409
76-77	22.036199321092255	27.88646180067054	27.609176097553796	22.468162780683414
78-79	22.158019874281504	27.898017648984197	27.747675050420266	22.196287426314033
80-81	22.130218375530617	27.68661937265055	27.844119059717148	22.33904319210168
82-83	21.924148845097506	27.862802498575444	27.90633582776789	22.30671282855916
84-85	21.952122235401124	27.76490944365587	27.723401301902733	22.559567019040276
86-87	22.083152957227437	27.753354050367403	27.76687044328098	22.39662254912419
88-89	21.964560817864477	27.727642401772574	27.808984375902075	22.498812404460878
90-91	21.94779629332761	27.69485027401987	27.866842916104	22.490510516548518
92-93	22.1102964910355	27.798710832937555	27.739710953572818	22.351281722454132
94-95	21.911850880488743	27.613260319910506	27.845984492661845	22.62890430693891
96-97	21.97403347471521	27.63061704998605	27.814749444926946	22.580600030371794
98-99	21.862700834305855	27.693184510032754	27.863987390876314	22.580127264785077
100	22.059845050614317	27.535884238101225	27.718874360818162	22.68539635046629
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	446.0
1	445.5
2	435.0
3	389.0
4	321.0
5	255.0
6	225.5
7	241.5
8	248.0
9	272.0
10	281.0
11	258.0
12	273.5
13	303.0
14	329.0
15	401.0
16	503.0
17	655.0
18	927.5
19	1309.0
20	1907.5
21	2890.0
22	4446.5
23	7334.5
24	11020.5
25	15145.0
26	20909.0
27	29259.0
28	39746.5
29	49820.5
30	58474.5
31	68682.5
32	79262.5
33	89235.5
34	99504.0
35	108330.5
36	116086.5
37	122736.0
38	127026.5
39	128684.0
40	131446.5
41	133574.5
42	132413.0
43	132157.5
44	130753.0
45	128211.0
46	126381.5
47	122081.5
48	118343.5
49	118137.5
50	116674.0
51	111601.5
52	103722.0
53	99620.5
54	99104.0
55	95336.0
56	87409.5
57	77657.5
58	69406.5
59	62728.5
60	56971.0
61	49904.5
62	42758.0
63	36565.5
64	30061.5
65	24129.5
66	18928.0
67	14374.0
68	11020.5
69	8600.0
70	6429.0
71	4699.0
72	3751.0
73	2927.0
74	2150.0
75	1478.0
76	873.0
77	559.0
78	392.5
79	254.0
80	179.0
81	113.0
82	69.5
83	55.0
84	46.5
85	35.5
86	24.0
87	21.0
88	18.0
89	11.0
90	6.5
91	9.5
92	8.5
93	6.0
94	4.5
95	3.5
96	4.0
97	3.0
98	1.5
99	3.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.327760212497286
2	0.11703985836394805
3	0.06438002361321
4	0.038698227280926985
5	0.0415877669313521
6	0.009829835820137769
7	0.00664324069163157
8	0.015149829382135407
9	0.017742313554479434
10-11	0.02068586329182838
12-13	0.01582495546868333
14-15	0.018363429554103524
16-17	0.019484138857773076
18-19	0.019065560684113365
20-21	0.023737433203025
22-23	0.015325362164637866
24-25	0.02488514755015647
26-27	0.01875500268430132
28-29	0.018971043031996655
30-31	0.01988921450970183
32-33	0.02484463998496359
34-35	0.01550089494714033
36-37	0.009843338341868728
38-39	0.013556531817882309
40-41	0.009870343385330644
42-43	0.00857410129915863
44-45	0.00938425260301614
46-47	0.00831755338627042
48-49	0.004280299388713836
50-51	0.006913291126250739
52-53	0.007858467647417832
54-55	0.0034566455631253696
56-57	0.002970554780810864
58-59	0.0031865951285061996
60-61	0.004037253997556584
62-63	0.0032676102588919506
64-65	0.002592484172344027
66-67	0.001998373216181854
68-69	0.003645680867358788
70-71	0.0027950219983084042
72-73	0.002362941302917733
74-75	0.0026059866940749852
76-77	0.004118269127942335
78-79	0.0036726859108207046
80-81	0.00398324391063275
82-83	0.0027545144331155287
84-85	0.0033486253892777013
86-87	0.002727509389653612
88-89	0.6010107447666926
90-91	0.6109080931954852
92-93	0.6075054577192837
94-95	0.595326183117959
96-97	0.5931657796410058
98-99	0.5905462904251998
100	0.5924636485109959
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3703012.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.927025282029625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.59591780634237	33.07267179472909
2	15.930914040890693	15.589044680889991
3	6.4786034973684625	9.50936391323977
4	3.2648230472691817	6.389523191003689
5	1.8657304774627317	4.564232112013614
6	1.1613915966196398	3.4094061606087522
7	0.8012829138105125	2.744307256744572
8	0.5529344736099561	2.164275117569605
9	0.39026493135747564	1.7185051946895087
>10	1.8381419841084996	15.350474299880968
>50	0.08783900607337057	2.9470998941158935
>100	0.031990740017103725	2.4886298190309843
>500	1.654850700238937E-4	0.05246656548348856
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.7005043461916947E-5	0.0	0.0	0.0
5	0.0	2.7005043461916947E-5	0.0	0.0	0.0
6	0.0	2.7005043461916947E-5	0.0	0.0	0.0
7	0.0	2.7005043461916947E-5	0.0	0.0	0.0
8	0.0	2.7005043461916947E-5	0.0	5.4010086923833894E-5	0.0
9	0.0	2.7005043461916947E-5	0.0	5.4010086923833894E-5	0.0
10-11	0.0	2.7005043461916947E-5	0.0	8.101513038575084E-5	0.0
12-13	0.0	2.7005043461916947E-5	0.0	8.101513038575084E-5	0.0
14-15	0.0	2.7005043461916947E-5	0.0	2.8355295635012795E-4	0.0
16-17	0.0	2.7005043461916947E-5	0.0	3.645680867358788E-4	0.0
18-19	0.0	2.7005043461916947E-5	0.0	3.780706084668373E-4	0.0
20-21	0.0	2.7005043461916947E-5	0.0	4.725882605835466E-4	0.0
22-23	0.0	2.7005043461916947E-5	0.0	5.13095825776422E-4	0.0
24-25	0.0	2.7005043461916947E-5	0.0	6.346185213550483E-4	0.0
26-27	0.0	2.7005043461916947E-5	0.0	9.046689559742177E-4	0.0
28-29	0.0	2.7005043461916947E-5	0.0	0.001795835390217477	0.0
30-31	0.0	2.7005043461916947E-5	0.0	0.005414511214114348	0.0
32-33	0.0	2.7005043461916947E-5	0.0	0.013921099904618187	0.0
34-35	0.0	2.7005043461916947E-5	0.0	0.027869204852698292	0.0
36-37	0.0	2.7005043461916947E-5	0.0	0.05144460779495179	0.0
38-39	0.0	2.7005043461916947E-5	0.0	0.09145257968378175	0.0
40-41	0.0	2.7005043461916947E-5	0.0	0.14070977895831827	0.0
42-43	0.0	2.7005043461916947E-5	0.0	0.18919733449419013	0.0
44-45	0.0	2.7005043461916947E-5	0.0	0.24597543837287053	0.0
46-47	0.0	2.7005043461916947E-5	0.0	0.31759281363387426	0.0
48-49	0.0	2.7005043461916947E-5	0.0	0.39547535897804276	0.0
50-51	0.0	2.7005043461916947E-5	0.0	0.47836733988439684	0.0
52-53	0.0	2.7005043461916947E-5	0.0	0.5627986082680801	0.0
54-55	0.0	2.7005043461916947E-5	0.0	0.6546562636037907	0.0
56-57	0.0	2.7005043461916947E-5	0.0	0.7639726795376305	0.0
58-59	0.0	2.7005043461916947E-5	0.0	0.8875207533759004	0.0
60-61	0.0	2.7005043461916947E-5	0.0	1.0201830293825673	0.0
62-63	0.0	2.7005043461916947E-5	0.0	1.1574091577342984	0.0
64-65	0.0	2.7005043461916947E-5	0.0	1.2990776157355148	0.0
66-67	0.0	2.7005043461916947E-5	0.0	1.4550047366846233	0.0
68-69	0.0	2.7005043461916947E-5	0.0	1.6339806622284776	0.0
70-71	0.0	2.7005043461916947E-5	0.0	1.8335074258468511	0.0
72-73	0.0	2.7005043461916947E-5	0.0	2.042094381546698	0.0
74-75	0.0	2.7005043461916947E-5	0.0	2.2473327118572666	0.0
76-77	0.0	2.7005043461916947E-5	0.0	2.472851829807735	0.0
78-79	0.0	2.7005043461916947E-5	0.0	2.727387866958033	0.0
80-81	0.0	2.7005043461916947E-5	0.0	3.0048781910509605	0.0
82-83	0.0	2.7005043461916947E-5	0.0	3.2971537764392878	0.0
84-85	0.0	2.7005043461916947E-5	0.0	3.589402356784153	0.0
86-87	0.0	2.7005043461916947E-5	0.0	3.8929120402526376	0.0
88	0.0	2.7005043461916947E-5	0.0	4.139225041668782	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4700	0.0	35.98215	1
TCAACGC	4710	0.0	34.50598	4
ATCAACG	4910	0.0	33.10536	3
CAACGCA	5015	0.0	32.406967	5
GGTATCA	3095	0.0	31.202095	1
GTACATG	11175	0.0	30.351175	1
AACGCAG	5435	0.0	29.981388	6
TACATGG	11200	0.0	28.74667	2
ACATGGG	11015	0.0	27.935759	3
TATCAAC	6120	0.0	27.033745	2
ACGCAGA	6180	0.0	25.455639	7
CGCAGAG	6085	0.0	25.312843	8
CATGGGG	7450	0.0	21.75214	4
GCAGAGT	7760	0.0	18.820292	9
GAGTACA	7245	0.0	17.945736	1
TATACTG	2075	0.0	17.65671	5
CATGGGA	4235	0.0	17.08073	4
AGAGTAC	6510	0.0	16.807482	10-11
AGTACAT	7635	0.0	16.252113	2
CTATACT	1975	0.0	16.172642	4
>>END_MODULE