FastQCFastQC Report
Wed 25 May 2016
SRR1033171_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033171_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3703012
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62020.16748527955080889No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49760.1343770962664987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47250.050.507211
GTATCAA87700.043.366961
TCAACGC105450.035.891994
ATCAACG105750.035.790173
CAACGCA109000.034.809775
AACGCAG113350.033.4170576
TATCAAC115750.032.7392042
ACGCAGA127650.029.3421697
CGCAGAG129850.028.4831148
GTACATG106900.027.5200711
TACATGG109150.025.6729982
ACATGGG107400.025.3029883
GCAGAGT147700.024.6905089
GTGGTAT21950.023.374331
AGAGTAC134900.020.91943210-11
GAGTACT95400.020.17197812-13
CAGAGTA147750.019.33859410-11
GAGTACA74250.019.2084961
CATGGGG71800.018.989864
AGTACTT102950.018.64698812-13
Produced by FastQC (version 0.11.5)