FastQCFastQC Report
Wed 25 May 2016
SRR1033170_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033170_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3249945
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC39000.033.2444344
GGTATCA27000.032.9780241
ATCAACG40150.032.0636563
GTATCAA43700.031.5873171
CAACGCA41350.031.1288385
AACGCAG43300.029.8235156
GTACATG89900.028.5603391
TACATGG90850.027.007282
TATCAAC49700.026.1028542
ACGCAGA51150.024.6956637
ACATGGG96550.024.6232993
CGCAGAG53050.023.6341448
CATGGGG62450.020.9868014
GCAGAGT66150.018.7407999
GTATAGA13050.018.0503651
GAGTACA64850.017.8711151
AGTACAT65850.016.131992
GTGTAGG12500.015.4525581
CAGAGTA65600.015.0324410-11
AGAGTAC56600.014.97527510-11
Produced by FastQC (version 0.11.5)