##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033170_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3249945
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.620326190135525	33.0	31.0	34.0	30.0	34.0
2	31.80192280177049	34.0	31.0	34.0	30.0	34.0
3	32.012141128542176	34.0	31.0	34.0	30.0	34.0
4	35.379811966048656	37.0	35.0	37.0	33.0	37.0
5	35.50747012641752	37.0	35.0	37.0	35.0	37.0
6	35.5660191172466	37.0	37.0	37.0	35.0	37.0
7	35.49366404662233	37.0	37.0	37.0	35.0	37.0
8	35.517027826624755	37.0	37.0	37.0	35.0	37.0
9	37.210526639681596	39.0	38.0	39.0	35.0	39.0
10-11	37.205290089524595	39.0	38.0	39.0	35.0	39.0
12-13	37.16695282535551	39.0	38.0	39.0	35.0	39.0
14-15	38.64917329370189	41.0	39.0	41.0	35.5	41.0
16-17	38.666235120902044	41.0	39.0	41.0	35.5	41.0
18-19	38.648477281923235	41.0	39.0	41.0	35.0	41.0
20-21	38.67233107021811	41.0	39.0	41.0	35.0	41.0
22-23	38.63925881822615	41.0	39.0	41.0	35.0	41.0
24-25	38.632277930857285	41.0	39.0	41.0	34.5	41.0
26-27	38.585408983844346	41.0	39.0	41.0	34.0	41.0
28-29	38.559831320222344	40.5	39.0	41.0	34.0	41.0
30-31	38.55769789950291	41.0	39.0	41.0	34.0	41.0
32-33	38.62190360144557	41.0	39.0	41.0	35.0	41.0
34-35	38.60215126717529	41.0	39.0	41.0	35.0	41.0
36-37	38.53018635699989	41.0	39.0	41.0	34.0	41.0
38-39	38.452149805612095	41.0	39.0	41.0	34.0	41.0
40-41	38.39240417914765	41.0	39.0	41.0	34.0	41.0
42-43	38.28875242504104	41.0	38.5	41.0	34.0	41.0
44-45	38.173508320910045	40.0	38.0	41.0	33.0	41.0
46-47	38.053178592253104	40.0	38.0	41.0	33.0	41.0
48-49	37.942275176964536	40.0	38.0	41.0	33.0	41.0
50-51	37.82364932329624	40.0	38.0	41.0	33.0	41.0
52-53	37.67897502880818	40.0	37.5	41.0	33.0	41.0
54-55	37.52963096298552	40.0	37.0	41.0	32.0	41.0
56-57	37.378652869510105	40.0	37.0	41.0	32.0	41.0
58-59	37.18678162245823	40.0	36.0	41.0	32.0	41.0
60-61	36.96829392497412	39.5	36.0	41.0	31.0	41.0
62-63	36.71460024708111	39.0	35.0	41.0	31.0	41.0
64-65	36.44433567337293	39.0	35.0	41.0	31.0	41.0
66-67	36.15529755118933	38.5	35.0	41.0	31.0	41.0
68-69	35.85082178313787	37.5	35.0	40.0	30.5	41.0
70-71	35.50025754281995	37.0	35.0	40.0	30.0	41.0
72-73	35.133276563141834	36.5	35.0	39.0	30.0	41.0
74-75	34.759471775676204	36.0	34.5	39.0	30.0	41.0
76-77	34.422165605879485	36.0	34.0	39.0	29.0	40.0
78-79	34.046176166058196	35.0	34.0	37.5	29.0	39.5
80-81	33.681559687933174	35.0	34.0	37.0	29.0	39.0
82-83	33.4504945468308	35.0	34.0	37.0	29.0	39.0
84-85	33.17326985533602	35.0	34.0	36.0	29.0	37.5
86-87	32.91524041176081	35.0	34.0	36.0	29.0	37.0
88-89	32.54954237687099	35.0	34.0	35.5	28.0	37.0
90-91	32.355583556029416	35.0	34.0	35.0	27.5	36.0
92-93	32.17474941883632	35.0	34.0	35.0	27.0	36.0
94-95	32.008917843225035	35.0	34.0	35.0	27.0	36.0
96-97	31.839811289114124	35.0	34.0	35.0	27.0	36.0
98-99	31.65277043149961	35.0	33.5	35.0	26.0	35.5
100	31.496989949060676	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.10981727291114396
1101	2	0.12207261125589497
1101	3	0.20668232672991138
1101	4	0.12808221846279366
1101	5	0.19764653245383812
1101	6	0.12724670863653387
1101	7	0.17078906694755602
1101	8	0.14788888770656428
1101	9	0.26174037771982483
1101	10-11	0.26591961472644243
1101	12-13	0.27905305742082476
1101	14-15	0.23348461271325505
1101	16-17	0.1975497933041268
1101	18-19	0.2368310777887075
1101	20-21	0.1996368756372746
1101	22-23	0.2407028164680156
1101	24-25	0.2210010996479781
1101	26-27	0.2258095676805283
1101	28-29	0.2547826057372262
1101	30-31	0.2509402869219244
1101	32-33	0.25543057545782233
1101	34-35	0.27632626138912997
1101	36-37	0.2457574472516484
1101	38-39	0.24096757525767742
1101	40-41	0.2811733135275105
1101	42-43	0.23561639168213588
1101	44-45	0.23746221013202984
1101	46-47	0.22680305560245984
1101	48-49	0.12798101836759201
1101	50-51	0.190603802572042
1101	52-53	0.18697595658618127
1101	54-55	0.13103213204273345
1101	56-57	0.17124771804049033
1101	58-59	0.13447442864364234
1101	60-61	0.19417347065054713
1101	62-63	0.22395999574797543
1101	64-65	0.18754545058803984
1101	66-67	0.1878582829409723
1101	68-69	0.13933219533499397
1101	70-71	0.10918627515452783
1101	72-73	0.1927991032937939
1101	74-75	0.14541121298563553
1101	76-77	0.12898110559279274
1101	78-79	0.07823484991669005
1101	80-81	0.07386463725742942
1101	82-83	0.0010741280691206612
1101	84-85	0.0052509750252980325
1101	86-87	0.12002087090458247
1101	88-89	0.26121061516190736
1101	90-91	0.2758968112040563
1101	92-93	0.32619660975552733
1101	94-95	0.28266750888670344
1101	96-97	0.28137617049542385
1101	98-99	0.36430885453651385
1101	100	0.4107190848769484
1102	1	0.07981780445033593
1102	2	0.10158862277149439
1102	3	0.17066503383347964
1102	4	0.13432438029071392
1102	5	0.07402183208294133
1102	6	0.020677162768301116
1102	7	0.16636968995327095
1102	8	0.1875746134188816
1102	9	0.14168575220311652
1102	10-11	0.18597800627956218
1102	12-13	0.20743830634944516
1102	14-15	0.1649225852231595
1102	16-17	0.21094548036263205
1102	18-19	0.27495917896591493
1102	20-21	0.1855613290629492
1102	22-23	0.20713170638903478
1102	24-25	0.12829100297980034
1102	26-27	0.18660016723584505
1102	28-29	0.17158505861650752
1102	30-31	0.17227344408135536
1102	32-33	0.21347724900896736
1102	34-35	0.202202395813309
1102	36-37	0.22965270919485192
1102	38-39	0.16564185662945619
1102	40-41	0.19134974740089916
1102	42-43	0.15944227035239322
1102	44-45	0.15647757207888446
1102	46-47	0.1306840286580666
1102	48-49	0.1387923572359
1102	50-51	0.10683580828759176
1102	52-53	0.07541516486667632
1102	54-55	0.15162329878982916
1102	56-57	0.1390795000453693
1102	58-59	0.16011524672191513
1102	60-61	0.1402273492270396
1102	62-63	0.13670856285526156
1102	64-65	0.19087186462735417
1102	66-67	0.11617363678707449
1102	68-69	0.14180176355572627
1102	70-71	0.18201309225838713
1102	72-73	0.17765545888934042
1102	74-75	0.16236554332401454
1102	76-77	0.14127471925385038
1102	78-79	0.20674605506459187
1102	80-81	0.184305976104838
1102	82-83	0.21155723568399054
1102	84-85	0.17106929701873952
1102	86-87	0.15315917693792613
1102	88-89	0.24740205677742466
1102	90-91	0.24683905494472214
1102	92-93	0.23815097258974305
1102	94-95	0.21076696845758391
1102	96-97	0.24612203673370558
1102	98-99	0.27839891979049014
1102	100	0.29034042935542814
1103	1	0.17674064802013234
1103	2	0.24170973719349576
1103	3	0.21905149766097765
1103	4	0.248543111137856
1103	5	0.21433871905251323
1103	6	0.29064882866040165
1103	7	0.23368083337958723
1103	8	0.23255644272814635
1103	9	0.30403741261005734
1103	10-11	0.28549836353801084
1103	12-13	0.32921202767693103
1103	14-15	0.327340385004284
1103	16-17	0.3356559001360111
1103	18-19	0.3634430793194525
1103	20-21	0.33083370864299155
1103	22-23	0.4171726113873149
1103	24-25	0.40279409786158027
1103	26-27	0.44240984986385
1103	28-29	0.4222616644143713
1103	30-31	0.38708873003714217
1103	32-33	0.3685012179181655
1103	34-35	0.3817214126470958
1103	36-37	0.46390393211188297
1103	38-39	0.36667832676223355
1103	40-41	0.42464161869543915
1103	42-43	0.34540348269595
1103	44-45	0.3255449143078195
1103	46-47	0.34530431369230996
1103	48-49	0.36293583029380727
1103	50-51	0.2565632258801358
1103	52-53	0.26209972149917604
1103	54-55	0.2715333227851957
1103	56-57	0.3133584044951334
1103	58-59	0.3106800990238696
1103	60-61	0.34396021141286326
1103	62-63	0.3510538183252123
1103	64-65	0.322666142072336
1103	66-67	0.31604362882389125
1103	68-69	0.2818451642130668
1103	70-71	0.28493554869238125
1103	72-73	0.3722189787033727
1103	74-75	0.3254319499012652
1103	76-77	0.20832328936467803
1103	78-79	0.22180786451441037
1103	80-81	0.22626688949841878
1103	82-83	0.28126648258579934
1103	84-85	0.3294368317573486
1103	86-87	0.3168124602287392
1103	88-89	0.4353402852475199
1103	90-91	0.4224007325567172
1103	92-93	0.4377301718447484
1103	94-95	0.40997435419740924
1103	96-97	0.43447121118203214
1103	98-99	0.4671117505645235
1103	100	0.4830599834173128
1104	1	0.16366898828558263
1104	2	0.08957563673487812
1104	3	0.16328907415429228
1104	4	0.14744155319428387
1104	5	0.16548461279084847
1104	6	0.1370170663052619
1104	7	0.13611415355322976
1104	8	0.09028512850846937
1104	9	0.11112594954550303
1104	10-11	0.1909402957365529
1104	12-13	0.20085453442735002
1104	14-15	0.1587213209877305
1104	16-17	0.19205320930553427
1104	18-19	0.17153115184422063
1104	20-21	0.1345726731572583
1104	22-23	0.13538888043534314
1104	24-25	0.2057932465809742
1104	26-27	0.23279091049612788
1104	28-29	0.25564954029415077
1104	30-31	0.22770634204078277
1104	32-33	0.16273529257156127
1104	34-35	0.14356487498293546
1104	36-37	0.24833608583931976
1104	38-39	0.20150607375763485
1104	40-41	0.23654268421361024
1104	42-43	0.23387983422897918
1104	44-45	0.161674908025347
1104	46-47	0.1152463019619816
1104	48-49	0.12232154295367081
1104	50-51	0.10393819039274632
1104	52-53	0.051812566456469256
1104	54-55	0.03172273897428113
1104	56-57	0.11132178233657442
1104	58-59	0.10060305437666273
1104	60-61	0.17027624662970453
1104	62-63	0.13715497397407717
1104	64-65	0.051284751683468244
1104	66-67	0.1790513382912451
1104	68-69	0.14209668595010783
1104	70-71	0.11350651868953321
1104	72-73	0.18390972538318806
1104	74-75	0.22079556595119953
1104	76-77	0.1322478493105521
1104	78-79	0.14971691381072816
1104	80-81	0.19098345516061954
1104	82-83	0.13570541904366706
1104	84-85	0.17302339953316448
1104	86-87	0.2280840179096657
1104	88-89	0.2926136276161557
1104	90-91	0.30122930665336156
1104	92-93	0.20852802646753332
1104	94-95	0.27421792133830536
1104	96-97	0.35346396112273126
1104	98-99	0.3555356787201056
1104	100	0.342136148018799
1105	1	0.13320634987639224
1105	2	0.165489149442255
1105	3	0.1736049653989582
1105	4	0.24567849577871215
1105	5	0.1514256713335982
1105	6	0.2030517655386035
1105	7	0.1878656667247327
1105	8	0.1881801214348542
1105	9	0.18242860165235442
1105	10-11	0.16994629844834463
1105	12-13	0.23652428110030144
1105	14-15	0.25563082306633333
1105	16-17	0.27137753704638357
1105	18-19	0.28966442937955605
1105	20-21	0.289706236667179
1105	22-23	0.3336938588032865
1105	24-25	0.3204029227528835
1105	26-27	0.32097245743270264
1105	28-29	0.33519796507787447
1105	30-31	0.31979871684869465
1105	32-33	0.35975586926434744
1105	34-35	0.2984431321493446
1105	36-37	0.2840978372354215
1105	38-39	0.27985162470827873
1105	40-41	0.28077364130178495
1105	42-43	0.2540314509297801
1105	44-45	0.25990181641382293
1105	46-47	0.23237438916190456
1105	48-49	0.2112615324610445
1105	50-51	0.18821852556243357
1105	52-53	0.19654600558170188
1105	54-55	0.12614103056585435
1105	56-57	0.1994810966429128
1105	58-59	0.195642138061757
1105	60-61	0.1771584999638307
1105	62-63	0.21422578854124907
1105	64-65	0.21793457172212527
1105	66-67	0.25968431701411987
1105	68-69	0.2212882819371771
1105	70-71	0.22106643439835238
1105	72-73	0.15376205743362448
1105	74-75	0.18034430356572528
1105	76-77	0.22470088951943978
1105	78-79	0.17282792969535166
1105	80-81	0.15449621664119206
1105	82-83	0.16791786874602366
1105	84-85	0.15731218506984135
1105	86-87	0.20234529696075043
1105	88-89	0.23902470068585302
1105	90-91	0.16492896978966343
1105	92-93	0.20824650332774297
1105	94-95	0.170465491119991
1105	96-97	0.19922299836720825
1105	98-99	0.2555937836008688
1105	100	0.23012559912762143
1106	1	0.005184301331702557
1106	2	0.020382978012165154
1106	3	0.031273998568956074
1106	4	0.05299621672377697
1106	5	0.02305465478693236
1106	6	0.057086091250546644
1106	7	-0.012468620837196909
1106	8	0.0824562123972612
1106	9	0.06639175183421742
1106	10-11	0.04101868272266529
1106	12-13	0.03952172897277251
1106	14-15	0.09472629745847172
1106	16-17	0.08747092159449465
1106	18-19	0.06043195531670875
1106	20-21	0.032177009400982115
1106	22-23	0.0021029734155604274
1106	24-25	0.05832269073084717
1106	26-27	0.11798687419890541
1106	28-29	0.10554612797935903
1106	30-31	0.03952123192792101
1106	32-33	0.035477592638656574
1106	34-35	0.05381697283540632
1106	36-37	0.037673870774625584
1106	38-39	0.07175016783180155
1106	40-41	0.0054341971327644956
1106	42-43	-0.0046376974693771444
1106	44-45	0.005510225654198564
1106	46-47	0.015170854429058522
1106	48-49	-0.021125254213430367
1106	50-51	-0.04420796937862548
1106	52-53	-0.015516573006152612
1106	54-55	0.06453799485862532
1106	56-57	-0.05973275895869534
1106	58-59	-0.06817780811816476
1106	60-61	-0.034777162529302075
1106	62-63	-0.0014138829285457177
1106	64-65	-0.03608659080195764
1106	66-67	0.0014354201316422177
1106	68-69	-0.10962284713491499
1106	70-71	-0.039759604164181894
1106	72-73	0.007227071853662892
1106	74-75	-0.018672161627613093
1106	76-77	-0.07911047530084403
1106	78-79	-0.019121420872878048
1106	80-81	-0.08263382841025901
1106	82-83	-0.1160931500587381
1106	84-85	-0.10917894508757797
1106	86-87	-0.08871540857244753
1106	88-89	0.09800444837639333
1106	90-91	0.0880771395802995
1106	92-93	0.08774831699085439
1106	94-95	0.10904104009532034
1106	96-97	0.13686687248886287
1106	98-99	0.15219646459914316
1106	100	0.18320108980492478
1107	1	0.21553598113950656
1107	2	0.2055397132172061
1107	3	0.23030317316929683
1107	4	0.25419169804717967
1107	5	0.18341289451363707
1107	6	0.23934372567563855
1107	7	0.14214389668076421
1107	8	0.24385172865009963
1107	9	0.2391443619577842
1107	10-11	0.17185415654901703
1107	12-13	0.21280006973925936
1107	14-15	0.24606954734451847
1107	16-17	0.25540735853621044
1107	18-19	0.23339267247772
1107	20-21	0.20874862113898018
1107	22-23	0.2635486048929465
1107	24-25	0.26588949328154854
1107	26-27	0.31275646769692855
1107	28-29	0.21660363802157434
1107	30-31	0.2385973482855519
1107	32-33	0.23695276504950868
1107	34-35	0.2623699995564266
1107	36-37	0.2701042568301091
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2216	18-19	-0.16881806252978748
2216	20-21	-0.13077007327621715
2216	22-23	-0.17598890714808846
2216	24-25	-0.17118739016117956
2216	26-27	-0.22142250136599984
2216	28-29	-0.1400967808946163
2216	30-31	-0.14883538990302725
2216	32-33	-0.12247083314937157
2216	34-35	-0.15731234099779812
2216	36-37	-0.12131636191134731
2216	38-39	-0.1559767521907034
2216	40-41	-0.19413521531590305
2216	42-43	-0.13987437611586273
2216	44-45	-0.12829164666226944
2216	46-47	-0.07172244267383121
2216	48-49	-0.021580982338086585
2216	50-51	-0.05477406367092641
2216	52-53	-0.03459738769164744
2216	54-55	-0.05822294021883323
2216	56-57	-0.12073506232748343
2216	58-59	-0.13025196531407346
2216	60-61	-0.11988088351416337
2216	62-63	-0.10820443328060492
2216	64-65	-0.033821844428644
2216	66-67	-0.042115868978179094
2216	68-69	-0.040378880569747366
2216	70-71	-0.01398969824543883
2216	72-73	0.010461944542242918
2216	74-75	-0.0034645041251692987
2216	76-77	-0.01887700346032517
2216	78-79	-0.0018379395191345793
2216	80-81	0.07580439832733532
2216	82-83	0.07548790331654942
2216	84-85	-0.009116402960913206
2216	86-87	-0.019040654797706225
2216	88-89	-0.014726458428476974
2216	90-91	0.005519764919931447
2216	92-93	0.0398600907818647
2216	94-95	0.02371037969491141
2216	96-97	0.030522411023280682
2216	98-99	0.036770711559437785
2216	100	0.04546321705484857
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26100.0
3	4751.0
4	3117.0
5	2635.0
6	2296.0
7	2530.0
8	3010.0
9	3393.0
10	3752.0
11	4126.0
12	4510.0
13	5087.0
14	5690.0
15	6006.0
16	5868.0
17	6145.0
18	5951.0
19	6130.0
20	6154.0
21	6616.0
22	7533.0
23	9785.0
24	14594.0
25	20765.0
26	23013.0
27	20660.0
28	21173.0
29	26678.0
30	32914.0
31	40080.0
32	52406.0
33	72438.0
34	102191.0
35	155775.0
36	294640.0
37	646442.0
38	1092529.0
39	501216.0
40	1246.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.15400120588643	19.70423366164358	13.294110637147044	23.847654495322946
2	15.329828951109834	20.02996890664322	40.80118150442802	23.839020637818926
3	18.191573184027963	25.22711870460512	29.932066836404637	26.649241274962275
4	12.08153166145571	16.59756516878178	39.344450522270655	31.97645264749186
5	12.74397474274217	38.00021671594081	36.038629616449725	13.217178924867293
6	30.924699404147365	38.310188639995516	17.908875136207854	12.856236819649258
7	27.518491632953435	32.34977981132487	22.567715979841914	17.56401257587978
8	24.864637798013806	36.88659241774711	20.43675352292556	17.812016261313524
9	24.848324071002246	17.02635778962147	21.143535253997552	36.98178288537873
10-11	23.724125153630638	26.979071148431625	29.140048512427636	20.1567551855101
12-13	24.41976550269448	24.19167857392352	29.143948502793883	22.24460742058811
14-15	21.64321762149082	26.351604728391102	26.042850717397215	25.962326932720863
16-17	21.466485972366208	29.73240388715357	26.56747868822339	22.233631452256837
18-19	21.372999430035293	28.77369262037273	28.424727727982805	21.428580221609174
20-21	22.60202774394443	28.075966917781496	27.90932474449303	21.41268059378104
22-23	22.154748798057504	27.851706185166464	27.96917303124935	22.02437198552668
24-25	22.077138056324603	27.93128722802297	27.867424342100705	22.124150373551714
26-27	21.79609887607253	27.931167136508563	28.00281289623675	22.269921091182155
28-29	21.78415989551051	27.956493693145834	28.062315769258937	22.197030642084716
30-31	22.062287166024735	28.366558387101243	27.71603465971954	21.855119787154482
32-33	21.753892774165394	28.049610345563053	28.211544457169097	21.984952423102456
34-35	21.842076553770855	27.83756839444563	28.233974976930654	22.086380074852862
36-37	22.192693974278217	28.17165643110777	27.65310333303689	21.982546261577127
38-39	21.94799199876904	28.342391137098016	27.860101554085244	21.8495153100477
40-41	21.756808696125805	28.06084752391887	28.146457243914767	22.035886536040554
42-43	21.793444427929913	27.830778145303064	28.170459521495	22.20531790527202
44-45	21.840595745348683	28.156365459780435	28.04684575400348	21.956193040867404
46-47	21.902807708250183	28.339709895893755	27.91385009082486	21.843632305031203
48-49	21.890862553664974	28.143816588877073	27.878138046988855	22.0871828104691
50-51	21.83095720225355	28.012052131948167	28.009836553384442	22.14715411241384
52-53	21.854719244335005	27.949923900527253	28.083382620025148	22.111974235112598
54-55	21.98623406399128	28.353191017324423	27.847042009998358	21.813532908685936
56-57	21.803520137655816	28.131592142331733	27.88278023763906	22.182107482373386
58-59	21.66495427625273	27.716862014565013	28.276319809910817	22.34186389927144
60-61	22.047048929797704	28.187940321176853	27.743609318581175	22.021401430444275
62-63	21.79968503181068	28.154112864216586	27.87860801045709	22.167594093515643
64-65	21.83913899431178	27.860126269510754	28.030025164052514	22.27070957212495
66-67	21.652039741092416	27.985078243525813	27.91226029347803	22.45062172190374
68-69	22.124443049503974	28.12076902247495	27.801491765748466	21.953296162272604
70-71	22.06782478336083	27.90589727478665	27.857541553918708	22.16873638793381
72-73	21.79755729630225	28.000104619166997	28.05442966014925	22.147908424381495
74-75	21.91195487200632	28.023742506425265	27.903383753337636	22.16091886823078
76-77	21.88889068386148	28.069560829185026	27.799253342687706	22.242295144265796
78-79	22.026365160644332	28.141921134684733	27.84315064931309	21.98856305535785
80-81	21.919733081586987	27.925997405404342	27.976830802330703	22.17743871067797
82-83	21.79873960898985	27.99472101067598	28.10654105732716	22.099998323007007
84-85	21.79015954582519	27.913057525731954	27.9721679462129	22.32461498222995
86-87	22.008129568458997	28.05896552626032	27.817232408115416	22.115672497165267
88-89	21.732161683035585	27.98034584538668	27.918291988087024	22.36920048349072
90-91	21.780502662232067	27.7973561121427	28.0300566348365	22.39208459078873
92-93	21.913386750678086	28.02469445396446	27.861259793618466	22.20065900173899
94-95	21.68672647566666	27.78389824999551	28.065912894141043	22.463462380196788
96-97	21.730703422803554	27.785888702973033	28.054789991869818	22.428617882353596
98-99	21.75115247313166	27.85802967256546	28.002303553359514	22.388514300943367
100	21.944271445322027	27.650539158815672	27.899843619799242	22.505345776063056
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	271.0
1	245.5
2	232.5
3	228.5
4	199.0
5	188.0
6	170.5
7	150.0
8	156.5
9	160.0
10	166.0
11	184.5
12	199.0
13	230.0
14	258.0
15	302.5
16	421.5
17	589.0
18	859.5
19	1233.0
20	1841.0
21	2754.0
22	4148.5
23	6375.0
24	9303.0
25	13031.5
26	18137.5
27	24871.0
28	33153.0
29	42423.0
30	51578.5
31	61178.5
32	71154.5
33	80291.0
34	89627.0
35	98426.5
36	105155.5
37	111715.0
38	116237.0
39	117709.0
40	119538.0
41	120888.5
42	118907.5
43	116683.0
44	115076.0
45	112023.5
46	110449.0
47	108976.0
48	106157.5
49	103859.5
50	101582.5
51	98532.5
52	92616.0
53	87041.0
54	83889.0
55	79838.5
56	73740.0
57	67010.5
58	60522.5
59	54553.5
60	48842.0
61	41617.5
62	34811.0
63	28954.0
64	23496.0
65	18877.0
66	14637.5
67	10954.0
68	8214.0
69	6192.0
70	4517.0
71	3236.5
72	2426.5
73	1765.5
74	1268.0
75	936.0
76	627.0
77	403.0
78	276.5
79	177.5
80	114.0
81	64.0
82	43.5
83	34.0
84	32.5
85	21.5
86	21.0
87	22.0
88	11.5
89	7.5
90	7.0
91	5.5
92	7.5
93	7.0
94	3.5
95	2.5
96	2.5
97	1.0
98	1.0
99	1.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3333594876220982
2	0.12043280732443164
3	0.06750883476489603
4	0.041293006497033026
5	0.04470844891221236
6	0.009723241470240266
7	0.0071385823452396885
8	0.015846422016372586
9	0.017938765117563527
10-11	0.02075419737872487
12-13	0.01618488928274171
14-15	0.0182926172596767
16-17	0.01927724930729597
18-19	0.019108015674111408
20-21	0.023615784267118366
22-23	0.01592334639509284
24-25	0.024508107060273326
26-27	0.01923109468006382
28-29	0.01916955517708761
30-31	0.01969264095238535
32-33	0.02412348516667205
34-35	0.015492569874259409
36-37	0.010030938985121286
38-39	0.013692539412205437
40-41	0.009661701967264062
42-43	0.008677069919644795
44-45	0.009277080073662784
46-47	0.008738609422620999
48-49	0.004707771977679622
50-51	0.007061657966519433
52-53	0.007723207623513628
54-55	0.0034308272909233852
56-57	0.0030923600245542616
58-59	0.003138514651786415
60-61	0.0035539062968757934
62-63	0.0028615868883934957
64-65	0.0021846523556552496
66-67	0.0018308002135420756
68-69	0.0032308239062507215
70-71	0.0021846523556552496
72-73	0.0019231094680063816
74-75	0.0023846557403279134
76-77	0.003923143314733019
78-79	0.0038769886875008653
80-81	0.003476981918155538
82-83	0.0024769649947922194
84-85	0.0029231263913697
86-87	0.0024615801190481686
88-89	0.6057487126705221
90-91	0.6167027442002865
92-93	0.6125642126251367
94-95	0.5981331991772169
96-97	0.5966716359815321
98-99	0.593363887696561
100	0.5956100795551924
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3249945.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.51260645380748
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.71366453647633	49.489984498711046
2	14.166332881078404	18.278141161012872
3	4.407187060112601	8.52957373132071
4	1.800186184652041	4.645388114961532
5	0.9375597843656285	3.024221269784819
6	0.5120272579373613	1.9819327790961097
7	0.3153178254698187	1.4239382347683334
8	0.20838229797674918	1.075462814505125
9	0.15253989098869797	0.8856671360274508
>10	0.7433860819180054	8.281889070114127
>50	0.034689917416767024	1.5144716546712795
>100	0.008630787666609609	0.8316268892962544
>500	9.549394093968316E-5	0.037702645730401045
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.15395029762042E-5	0.0
10-11	0.0	0.0	0.0	7.692437872025527E-5	0.0
12-13	0.0	0.0	0.0	9.230925446430632E-5	0.0
14-15	0.0	0.0	0.0	9.230925446430632E-5	0.0
16-17	0.0	0.0	0.0	9.230925446430632E-5	0.0
18-19	0.0	0.0	0.0	9.230925446430632E-5	0.0
20-21	0.0	0.0	0.0	1.2307900595240844E-4	0.0
22-23	0.0	0.0	0.0	2.0000338467266368E-4	0.0
24-25	0.0	0.0	0.0	2.461580119048168E-4	0.0
26-27	0.0	0.0	0.0	3.2308239062507215E-4	0.0
28-29	0.0	0.0	0.0	0.0012154051837800332	0.0
30-31	0.0	0.0	0.0	0.004400074462798602	0.0
32-33	0.0	0.0	0.0	0.012461749352681354	0.0
34-35	0.0	0.0	0.0	0.023292701876493294	0.0
36-37	0.0	0.0	0.0	0.04226225366890825	0.0
38-39	0.0	0.0	0.0	0.07489357512204053	0.0
40-41	0.0	0.0	0.0	0.11550964708633531	0.0
42-43	0.0	0.0	0.0	0.15501800799705842	0.0
44-45	0.0	0.0	0.0	0.1996495325305505	0.0
46-47	0.0	0.0	0.0	0.2540966077887472	0.0
48-49	0.0	1.538487574405105E-5	0.0	0.317943842126559	0.0
50-51	0.0	3.07697514881021E-5	0.0	0.38643730893907435	0.0
52-53	0.0	3.07697514881021E-5	0.0	0.45391537395248227	0.0
54-55	0.0	3.07697514881021E-5	0.0	0.5289320280804752	0.0
56-57	0.0	3.07697514881021E-5	0.0	0.616841208081983	0.0
58-59	0.0	3.07697514881021E-5	0.0	0.7192275561586428	0.0
60-61	0.0	3.07697514881021E-5	0.0	0.8287524865805422	0.0
62-63	0.0	3.07697514881021E-5	0.0	0.9390620456653882	0.0
64-65	0.0	3.07697514881021E-5	0.0	1.0529870505500862	0.0
66-67	0.0	3.07697514881021E-5	0.0	1.1868200846475863	0.0
68-69	0.0	3.07697514881021E-5	0.0	1.3384841897324415	0.0
70-71	0.0	3.07697514881021E-5	0.0	1.5050100847860501	0.0
72-73	0.0	3.07697514881021E-5	0.0	1.6814746095703157	0.0
74-75	0.0	3.07697514881021E-5	0.0	1.8578006704728849	0.0
76-77	0.0	3.07697514881021E-5	0.0	2.0493116037348327	0.0
78-79	0.0	3.07697514881021E-5	0.0	2.268807625975209	0.0
80-81	0.0	3.07697514881021E-5	0.0	2.5087039934521966	0.0
82-83	0.0	3.07697514881021E-5	0.0	2.7583236023994253	0.0
84-85	0.0	3.07697514881021E-5	0.0	3.0055124009790934	0.0
86-87	0.0	3.07697514881021E-5	0.0	3.2662245053377825	0.0
88	0.0	6.15395029762042E-5	0.0	3.4812281438608963	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	3900	0.0	33.244434	4
GGTATCA	2700	0.0	32.978024	1
ATCAACG	4015	0.0	32.063656	3
GTATCAA	4370	0.0	31.587317	1
CAACGCA	4135	0.0	31.128838	5
AACGCAG	4330	0.0	29.823515	6
GTACATG	8990	0.0	28.560339	1
TACATGG	9085	0.0	27.00728	2
TATCAAC	4970	0.0	26.102854	2
ACGCAGA	5115	0.0	24.695663	7
ACATGGG	9655	0.0	24.623299	3
CGCAGAG	5305	0.0	23.634144	8
CATGGGG	6245	0.0	20.986801	4
GCAGAGT	6615	0.0	18.740799	9
GTATAGA	1305	0.0	18.050365	1
GAGTACA	6485	0.0	17.871115	1
AGTACAT	6585	0.0	16.13199	2
GTGTAGG	1250	0.0	15.452558	1
CAGAGTA	6560	0.0	15.03244	10-11
AGAGTAC	5660	0.0	14.975275	10-11
>>END_MODULE