FastQCFastQC Report
Wed 25 May 2016
SRR1033170_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033170_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3249945
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48420.1489871367053904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40890.12581751383484951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42700.049.371771
GTATCAA77250.041.9709971
TCAACGC88600.035.9763074
ATCAACG88700.035.9357453
CAACGCA91600.034.798045
AACGCAG95750.033.3266146
TATCAAC97850.032.912212
ACGCAGA111300.028.4593457
CGCAGAG113700.027.77518
GCAGAGT127650.024.4816659
GTACATG87550.024.4559081
TACATGG88250.023.706782
ACATGGG91000.021.8534283
GAGTACT84850.021.5165512-13
CAGAGTA125150.020.1264610-11
AGAGTAC117750.019.8747610-11
GTGGTAT25200.019.4205421
TGGTATC23700.019.242022
GTACTTT95200.019.07857914-15
GAGTACA69000.018.8911841
Produced by FastQC (version 0.11.5)