##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033170_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3249945
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.77461710890492	34.0	31.0	34.0	31.0	34.0
2	32.87013410996186	34.0	31.0	34.0	31.0	34.0
3	32.97469864874636	34.0	31.0	34.0	31.0	34.0
4	36.39239587131475	37.0	37.0	37.0	35.0	37.0
5	36.32126666758976	37.0	37.0	37.0	35.0	37.0
6	36.40420468654085	37.0	37.0	37.0	35.0	37.0
7	36.384215732881636	37.0	37.0	37.0	35.0	37.0
8	36.37456787730254	37.0	37.0	37.0	35.0	37.0
9	38.198853826757066	39.0	39.0	39.0	37.0	39.0
10-11	38.18748901904494	39.0	39.0	39.0	37.0	39.0
12-13	38.148710208942	39.0	39.0	39.0	37.0	39.0
14-15	39.70230280820137	41.0	40.0	41.0	37.0	41.0
16-17	39.70939323588553	41.0	40.0	41.0	37.0	41.0
18-19	39.71862939218971	41.0	40.0	41.0	37.0	41.0
20-21	39.71343238116337	41.0	40.0	41.0	37.0	41.0
22-23	39.64488645192458	41.0	40.0	41.0	37.0	41.0
24-25	39.60204264995254	41.0	40.0	41.0	37.0	41.0
26-27	39.50884722664537	41.0	39.5	41.0	37.0	41.0
28-29	39.42381747998812	41.0	39.0	41.0	36.0	41.0
30-31	39.34026745067993	41.0	39.0	41.0	36.0	41.0
32-33	39.38385849606685	41.0	39.0	41.0	36.0	41.0
34-35	39.33091960017785	41.0	39.0	41.0	36.0	41.0
36-37	39.25047500803859	41.0	39.0	41.0	35.0	41.0
38-39	39.16664851251329	41.0	39.0	41.0	35.0	41.0
40-41	39.061771506902424	41.0	39.0	41.0	35.0	41.0
42-43	38.95749851151327	41.0	39.0	41.0	35.0	41.0
44-45	38.82856525264274	41.0	39.0	41.0	35.0	41.0
46-47	38.63918235539371	41.0	39.0	41.0	35.0	41.0
48-49	38.47092689260895	41.0	39.0	41.0	35.0	41.0
50-51	38.346922486380535	41.0	38.0	41.0	35.0	41.0
52-53	38.215244873374786	40.5	38.0	41.0	34.0	41.0
54-55	38.08029335881069	40.0	38.0	41.0	34.0	41.0
56-57	37.923621015124866	40.0	37.5	41.0	34.0	41.0
58-59	37.736708928920336	40.0	37.0	41.0	34.0	41.0
60-61	37.55022438841273	40.0	36.5	41.0	33.5	41.0
62-63	37.31742075635126	39.5	36.0	41.0	33.0	41.0
64-65	37.0650618702778	39.0	35.5	41.0	33.0	41.0
66-67	36.78583837572636	39.0	35.0	41.0	33.0	41.0
68-69	36.46539510668642	38.5	35.0	41.0	32.5	41.0
70-71	36.12869756257413	37.0	35.0	40.0	32.0	41.0
72-73	35.77552835509524	37.0	35.0	39.5	32.0	41.0
74-75	35.43457750823475	36.0	35.0	39.0	31.5	41.0
76-77	35.08741301775876	36.0	35.0	39.0	31.0	40.5
78-79	34.744599370143185	35.5	35.0	37.5	31.0	39.5
80-81	34.402776200828015	35.0	35.0	37.0	31.0	39.0
82-83	34.07903595291613	35.0	34.5	37.0	31.0	39.0
84-85	33.804695156379566	35.0	34.0	36.0	31.0	37.5
86-87	33.56989979830428	35.0	34.0	36.0	30.5	37.0
88-89	33.352878279478574	35.0	34.0	36.0	30.0	37.0
90-91	33.15489292895725	35.0	34.0	35.0	30.0	36.0
92-93	32.99647624805958	35.0	34.0	35.0	30.0	36.0
94-95	32.850725166118195	35.0	34.0	35.0	30.0	36.0
96-97	32.72945188303187	35.0	34.0	35.0	30.0	36.0
98-99	32.62834786434847	35.0	34.0	35.0	30.0	36.0
100	32.534359812242975	35.0	34.0	35.0	30.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.14620158157312346
1101	2	0.15744823899699867
1101	3	0.15575814765971074
1101	4	0.14058984685360798
1101	5	0.08925303738328694
1101	6	0.13442399549234807
1101	7	0.14063915033263896
1101	8	0.11809722264662526
1101	9	0.20761340909526638
1101	10-11	0.20774164605474965
1101	12-13	0.20024204895896958
1101	14-15	0.21914407589363094
1101	16-17	0.23469372968484947
1101	18-19	0.2541799415136836
1101	20-21	0.2937370949848699
1101	22-23	0.3153055503451725
1101	24-25	0.24353613807415542
1101	26-27	0.33308896764956586
1101	28-29	0.320606495946798
1101	30-31	0.3302678249454587
1101	32-33	0.2944871130526394
1101	34-35	0.27605294220595766
1101	36-37	0.28266210808333625
1101	38-39	0.23305220466436793
1101	40-41	0.26660741035881586
1101	42-43	0.22402440390786893
1101	44-45	0.19756522164243506
1101	46-47	0.15485266723946012
1101	48-49	0.06825220515275277
1101	50-51	0.12918116663823298
1101	52-53	0.20960093571560634
1101	54-55	0.18933555409164882
1101	56-57	0.1633778606581302
1101	58-59	0.15867983491911986
1101	60-61	0.20570223113002584
1101	62-63	0.11753452464967751
1101	64-65	0.10290258079380266
1101	66-67	0.13257935231916917
1101	68-69	0.1292690108126351
1101	70-71	0.11320980226455646
1101	72-73	0.14464985811639508
1101	74-75	0.13940436005746193
1101	76-77	0.1186575449258811
1101	78-79	0.1030095477819657
1101	80-81	0.09303602196268201
1101	82-83	0.11836315485393101
1101	84-85	0.1764439065915795
1101	86-87	0.15688235880998036
1101	88-89	0.2130105585543447
1101	90-91	0.1847949740912327
1101	92-93	0.1737745258363077
1101	94-95	0.23402464321522842
1101	96-97	0.3101303264354769
1101	98-99	0.3359365016342153
1101	100	0.3418663332207146
1102	1	0.07064374976094712
1102	2	0.10228961632877542
1102	3	0.08942066391053771
1102	4	0.06529557956181264
1102	5	0.034482333873761206
1102	6	0.044276283304341746
1102	7	0.056474561659250355
1102	8	0.07699438791794222
1102	9	0.11443151336654722
1102	10-11	0.07285777344124966
1102	12-13	0.08117518109659727
1102	14-15	0.12171520523800439
1102	16-17	0.10197660462946345
1102	18-19	0.13154653852318887
1102	20-21	0.1743908208773064
1102	22-23	0.12872615475397708
1102	24-25	0.13101538425409842
1102	26-27	0.16572790537893667
1102	28-29	0.15903105788537886
1102	30-31	0.1356045944823734
1102	32-33	0.20452851508125747
1102	34-35	0.23465036231434055
1102	36-37	0.2417858738504961
1102	38-39	0.20639394745835205
1102	40-41	0.244319641307726
1102	42-43	0.20033821095171334
1102	44-45	0.25538757520977384
1102	46-47	0.20827087624019924
1102	48-49	0.2048747505848425
1102	50-51	0.27118209369828605
1102	52-53	0.27229398134928573
1102	54-55	0.20537440101834648
1102	56-57	0.22941060881092312
1102	58-59	0.2173091879987865
1102	60-61	0.1938256378180867
1102	62-63	0.1450237760684061
1102	64-65	0.18796469312126618
1102	66-67	0.11461802794633513
1102	68-69	0.17746928642382898
1102	70-71	0.1805690482495237
1102	72-73	0.2236189123224932
1102	74-75	0.21175963922811292
1102	76-77	0.1826828041789028
1102	78-79	0.22199816448719645
1102	80-81	0.17833509283681082
1102	82-83	0.227277072894303
1102	84-85	0.1956753252928891
1102	86-87	0.17872571815624383
1102	88-89	0.13987674275054474
1102	90-91	0.15224385638501303
1102	92-93	0.14531483820958613
1102	94-95	0.19430430362435658
1102	96-97	0.16488445706366406
1102	98-99	0.18327154053523032
1102	100	0.20177697428715646
1103	1	0.08735099660808032
1103	2	0.13191608759915852
1103	3	0.06946839890152745
1103	4	0.05851893928328877
1103	5	0.006743921271649356
1103	6	0.07275259085771779
1103	7	0.09670471786281354
1103	8	0.06758363683039192
1103	9	0.10511754622987723
1103	10-11	0.05736958549255178
1103	12-13	0.1131057276988443
1103	14-15	0.1005894072664546
1103	16-17	0.13069828926105487
1103	18-19	0.1342648668020061
1103	20-21	0.16866017480144535
1103	22-23	0.15252839273402685
1103	24-25	0.1574221907893758
1103	26-27	0.18174631064398028
1103	28-29	0.16750593682828452
1103	30-31	0.22810326508095358
1103	32-33	0.22150230360634282
1103	34-35	0.20612478092719755
1103	36-37	0.2734734684849158
1103	38-39	0.2432729318044906
1103	40-41	0.3172894491243454
1103	42-43	0.23921129742938518
1103	44-45	0.21533279858007148
1103	46-47	0.25836296429552164
1103	48-49	0.23245773565763272
1103	50-51	0.19828823592968803
1103	52-53	0.2184671130662963
1103	54-55	0.20502375210289614
1103	56-57	0.21160081859619595
1103	58-59	0.24524502663282277
1103	60-61	0.2179476596722978
1103	62-63	0.1938744280703517
1103	64-65	0.21941396554405657
1103	66-67	0.24607091598825548
1103	68-69	0.29803023209341717
1103	70-71	0.29430187516101824
1103	72-73	0.29308542772105994
1103	74-75	0.23873965367294403
1103	76-77	0.15198355225932403
1103	78-79	0.19550074700929088
1103	80-81	0.20718343436995923
1103	82-83	0.17285417861765495
1103	84-85	0.1881172483084228
1103	86-87	0.24926347095195212
1103	88-89	0.21757827162831944
1103	90-91	0.14270214933658565
1103	92-93	0.16873093247670568
1103	94-95	0.14918424302305056
1103	96-97	0.24212080567637173
1103	98-99	0.1988356785319425
1103	100	0.19612933312588865
1104	1	0.03267372659246348
1104	2	0.06059955420390395
1104	3	0.07713294681607863
1104	4	0.06188904332545064
1104	5	0.021670930837927926
1104	6	0.05274687118584609
1104	7	0.07475975011100644
1104	8	0.11981441308407881
1104	9	0.11014289496519325
1104	10-11	0.07011518849479614
1104	12-13	0.07881343912555394
1104	14-15	0.1331044078563437
1104	16-17	0.16449526550564997
1104	18-19	0.14879896159096972
1104	20-21	0.12437956528118832
1104	22-23	0.10763496122689276
1104	24-25	0.12579817917326608
1104	26-27	0.13241966443528952
1104	28-29	0.11636364915130315
1104	30-31	0.12372274473255374
1104	32-33	0.07525348287177991
1104	34-35	0.04672717660259451
1104	36-37	0.048174154970602956
1104	38-39	0.06061013480989885
1104	40-41	0.06241089626802676
1104	42-43	0.023847725152457144
1104	44-45	0.09362650831673847
1104	46-47	0.13939441457001323
1104	48-49	0.0927413575939866
1104	50-51	0.13937702257967288
1104	52-53	0.06647761983672495
1104	54-55	0.02212405980623089
1104	56-57	0.0887815477209557
1104	58-59	0.06864510267278234
1104	60-61	0.02626085047796778
1104	62-63	0.10224816110258672
1104	64-65	0.08244272773952588
1104	66-67	0.10372727209869481
1104	68-69	0.12301228516380291
1104	70-71	0.14647243227457096
1104	72-73	0.2198082808534707
1104	74-75	0.15219610933569783
1104	76-77	0.1861825105453221
1104	78-79	0.15588394209638068
1104	80-81	0.20227981626061364
1104	82-83	0.20343899839171087
1104	84-85	0.1594825156426296
1104	86-87	0.14158601000268334
1104	88-89	0.14709160134030697
1104	90-91	0.1642979231994275
1104	92-93	0.18319400036362765
1104	94-95	0.16933529947396408
1104	96-97	0.11636832697818988
1104	98-99	0.15649363965235352
1104	100	0.16112708819636623
1105	1	0.032413870429707003
1105	2	0.044950832122701456
1105	3	0.05670002831804766
1105	4	0.03312577469989719
1105	5	0.017415841508494623
1105	6	0.025708844829551936
1105	7	-0.0167802028600903
1105	8	-0.013059049113905985
1105	9	0.036687957014393646
1105	10-11	0.034289321537805506
1105	12-13	0.027466687711253712
1105	14-15	0.03420538049457633
1105	16-17	0.020717288421202795
1105	18-19	0.014956461675332378
1105	20-21	0.012037937110918051
1105	22-23	0.04347685338505869
1105	24-25	-0.009281379703807602
1105	26-27	-0.04457521487842797
1105	28-29	-0.05012859957989235
1105	30-31	0.03178538349589388
1105	32-33	0.08950509072871426
1105	34-35	0.07799928933894762
1105	36-37	-0.047553574598765636
1105	38-39	-0.04387990718517898
1105	40-41	0.058275651636790826
1105	42-43	0.040674112608762414
1105	44-45	0.10363084761461039
1105	46-47	0.17627527393790388
1105	48-49	0.11376046496568648
1105	50-51	0.0787094130920849
1105	52-53	0.11910734649637789
1105	54-55	0.13948192517467106
1105	56-57	0.11290754220077304
1105	58-59	0.0655758192058542
1105	60-61	0.07990808887541334
1105	62-63	0.038215652865453364
1105	64-65	-0.0045272884106140054
1105	66-67	0.03642290276708593
1105	68-69	0.019154249951846225
1105	70-71	0.04544841798429644
1105	72-73	0.03568946672623241
1105	74-75	0.0817264676479752
1105	76-77	0.03292996527805769
1105	78-79	0.04731893442661317
1105	80-81	0.08144114242323752
1105	82-83	0.049415271059771726
1105	84-85	0.025611929815774204
1105	86-87	0.07225421662329978
1105	88-89	0.11366853081535311
1105	90-91	0.06304993483438182
1105	92-93	0.10506231551096334
1105	94-95	0.08573396949046952
1105	96-97	0.13552395135652517
1105	98-99	0.10557501393189028
1105	100	0.14523764378684234
1106	1	0.011741104409267678
1106	2	0.039835915305708625
1106	3	0.03220761410054962
1106	4	0.02403852764189196
1106	5	-0.02992037624425592
1106	6	0.002323067879565599
1106	7	0.0157379286452084
1106	8	0.012534671485056492
1106	9	0.0363981755164815
1106	10-11	0.023236412767175807
1106	12-13	0.03676129256751892
1106	14-15	0.04709607805741456
1106	16-17	0.05118569424160313
1106	18-19	0.08855522119181813
1106	20-21	0.12014980971859757
1106	22-23	0.09389835560961046
1106	24-25	0.08240357258544861
1106	26-27	0.04766039060156402
1106	28-29	0.08630517707812402
1106	30-31	0.09757630860554656
1106	32-33	0.06557139437570925
1106	34-35	0.07676881148788794
1106	36-37	0.0617155103491811
1106	38-39	0.03310355490272343
1106	40-41	0.09227228388370179
1106	42-43	0.02984503270739225
1106	44-45	-0.0032575866453825597
1106	46-47	-0.017782168682963118
1106	48-49	-0.013474184372043396
1106	50-51	-0.10394731461596507
1106	52-53	-0.11290204078388655
1106	54-55	-0.06072127726626775
1106	56-57	-0.10210889401442103
1106	58-59	-0.10333063973668999
1106	60-61	-0.09013589913583786
1106	62-63	-0.16833642982287955
1106	64-65	-0.14403466259794584
1106	66-67	-0.14162192506374538
1106	68-69	-0.10991805148871236
1106	70-71	-0.11430750671460999
1106	72-73	-0.05921028322786981
1106	74-75	-0.0848778686587579
1106	76-77	-0.0524038513889451
1106	78-79	-0.10063317836072372
1106	80-81	-0.08500545433228268
1106	82-83	-0.08030425462259672
1106	84-85	-0.08227057800251458
1106	86-87	-0.02962291137414752
1106	88-89	-0.06590714979185464
1106	90-91	-0.15224097366752432
1106	92-93	-0.09053923875670478
1106	94-95	-0.0987761295997629
1106	96-97	-0.07268234627887438
1106	98-99	-0.01968044144400949
1106	100	0.0012852674623786697
1107	1	-0.012145690133216647
1107	2	-0.0020824775993588673
1107	3	0.013297242187221059
1107	4	0.021589036887547763
1107	5	-0.0768798964330486
1107	6	-0.10047401338249529
1107	7	-0.08204511694622596
1107	8	-0.0380681595033181
1107	9	-0.03740619407105328
1107	10-11	-0.02338510121099091
1107	12-13	0.0021695197281417222
1107	14-15	0.02765117924629834
1107	16-17	0.02147846256996644
1107	18-19	0.07433169834498443
1107	20-21	0.03693444843318616
1107	22-23	0.07894346570124355
1107	24-25	0.06918001140142849
1107	26-27	0.09903396946329224
1107	28-29	0.04455137850171553
1107	30-31	0.08428895859979946
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2216	1	-0.006221722302399257
2216	2	-0.04466586880435841
2216	3	-0.08950568798594816
2216	4	-0.04187923077078182
2216	5	-0.014880971725951042
2216	6	-0.006535081058743231
2216	7	-0.04811850568701459
2216	8	-0.031216079483598946
2216	9	-0.03160319669208178
2216	10-11	-0.07358291030169539
2216	12-13	-0.0013961963497308716
2216	14-15	-0.09588059789925296
2216	16-17	-0.07065308159544514
2216	18-19	-0.07866008014467951
2216	20-21	-0.09183960564507743
2216	22-23	-0.08239090558843998
2216	24-25	-0.174791627748192
2216	26-27	-0.18043264045180507
2216	28-29	-0.2079928093161172
2216	30-31	-0.11953897213341946
2216	32-33	-0.13729714143906335
2216	34-35	-0.033466629113831914
2216	36-37	-0.11579382637402347
2216	38-39	-0.06153661299087787
2216	40-41	-0.11522162973111705
2216	42-43	-0.05462991209774515
2216	44-45	0.04519758714942412
2216	46-47	-0.03724627810066039
2216	48-49	-0.06012996279093841
2216	50-51	0.03228091269344446
2216	52-53	0.08815808230313138
2216	54-55	0.1129642440448464
2216	56-57	0.09337143576993867
2216	58-59	0.07964826911922529
2216	60-61	0.06685775111012049
2216	62-63	0.19967649310606106
2216	64-65	0.1794880537806094
2216	66-67	0.1514292610584249
2216	68-69	0.19925149557543165
2216	70-71	0.107042852688231
2216	72-73	0.1273702803301049
2216	74-75	0.18046175148717936
2216	76-77	0.17577110500619142
2216	78-79	0.1920189531203249
2216	80-81	0.24500037797960061
2216	82-83	0.2122898123133936
2216	84-85	0.2076457936690943
2216	86-87	0.2203253329539976
2216	88-89	0.2128207175599215
2216	90-91	0.22625930947077677
2216	92-93	0.21023852502317908
2216	94-95	0.16709687263539053
2216	96-97	0.175930020742598
2216	98-99	0.19918179983368134
2216	100	0.2162235532628074
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	13.0
3	3.0
4	12.0
5	27.0
6	57.0
7	135.0
8	285.0
9	609.0
10	1174.0
11	1831.0
12	2791.0
13	4287.0
14	5288.0
15	6985.0
16	8563.0
17	12147.0
18	14835.0
19	8271.0
20	5720.0
21	5324.0
22	5608.0
23	6070.0
24	6837.0
25	8562.0
26	10689.0
27	13559.0
28	17681.0
29	23095.0
30	29791.0
31	37600.0
32	48586.0
33	64109.0
34	88863.0
35	137462.0
36	261225.0
37	620888.0
38	1177272.0
39	611173.0
40	2518.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.89425356809476	19.76291645224067	13.505557829420695	23.83727215024387
2	15.549180686011999	20.152748657349086	40.286235266533204	24.011835390105706
3	18.189292434179656	25.280550901630644	30.198726439985908	26.331430224203796
4	12.217798549449697	16.732990988446243	38.99513190862967	32.05407855347439
5	12.48947246588094	37.80742246563468	36.024944715054744	13.678160353429636
6	30.84768897414169	38.331299450882504	17.547113148480804	13.273898426494995
7	27.172259768041613	32.562487556953386	22.524445297828454	17.74080737717655
8	25.119248575509534	36.34492269689169	20.274073258103087	18.26175546949569
9	25.024023513042238	16.280634017663704	21.30235595046187	37.39298651883219
10-11	23.847504882417358	26.58282786044117	29.038436863351087	20.531230393790384
12-13	24.831589457667747	23.858926843377347	29.269326096287784	22.040157602667122
14-15	21.86875277504867	25.87993081724818	26.628497792115123	25.622818615588027
16-17	21.09894167439757	29.44397520573425	27.112166513587155	22.34491660628103
18-19	20.888049694425316	27.99537653287351	29.792485527434025	21.32408824526715
20-21	22.089050737781715	27.30227742315639	29.467652529504345	21.141019309557546
22-23	21.624470739984773	27.108528361382838	29.544035987142525	21.722964911489864
24-25	21.54858787458174	27.220197655865498	29.485315351060937	21.745899118491824
26-27	21.28861642209221	27.193312156248666	29.63152826559246	21.88654315606666
28-29	21.300027141083785	27.24846339000137	29.610953181322746	21.840556287592094
30-31	21.61418358945157	27.638915607950565	29.222934445530306	21.523966357067557
32-33	21.30554980621959	27.36951849973878	29.694637164750663	21.630294529290964
34-35	21.364443168181406	27.178154726784808	29.680040651645136	21.777361453388647
36-37	21.78287299228198	27.53371729028124	29.10929908108246	21.57411063635432
38-39	21.573855941208304	27.682468719285392	29.19037851707507	21.553296822431232
40-41	21.46765192190022	27.421550570457363	29.361841222392805	21.74895628524961
42-43	21.538752257645758	27.25224833587829	29.248994340670492	21.96000506580546
44-45	21.607702979852817	27.578380248226686	29.097343787315864	21.716572984604625
46-47	21.703266436352468	27.81345534692692	28.783458492051327	21.699819724669283
48-49	21.715286098221956	27.751442134816507	28.550322738778306	21.98294902818323
50-51	21.75940585376211	27.669019187895515	28.482431482242234	22.089143476100137
52-53	21.757400584717043	27.6951926239862	28.49995553541058	22.047451255886173
54-55	21.864455178720775	28.115312919061928	28.289261121699738	21.730970780517556
56-57	21.646947398155064	27.81259971561913	28.385638550746844	22.15481433547896
58-59	21.49702161257855	27.364917153166868	28.83098716796419	22.30707406629039
60-61	21.841726144980623	27.84075873774279	28.29341547679269	22.0240996404839
62-63	21.62614567004303	27.76990488475105	28.414663284203094	22.189286161002826
64-65	21.655881814907545	27.51550340069385	28.573905607687	22.25470917671161
66-67	21.439870569559787	27.698818836461452	28.39995679908281	22.461353794895953
68-69	21.999751378036464	27.74458031812534	28.279717555603014	21.975950748235185
70-71	21.92350000846171	27.576766919953783	28.294319732548495	22.205413339036014
72-73	21.727987721583585	27.703761665057147	28.384191600404996	22.184059012954275
74-75	21.824341673978363	27.720025225170335	28.21074371328477	22.244889387566527
76-77	21.786521334573976	27.725576749819076	28.135230970702192	22.352670944904762
78-79	21.90124902209808	27.839477159980525	28.202929744141624	22.056344073779766
80-81	21.773505815288942	27.658196367330994	28.290918758266535	22.277379059113528
82-83	21.66935698267777	27.671905346826147	28.412460214503216	22.24627745599287
84-85	21.629335698096394	27.586957043488212	28.276847127545203	22.506860130870187
86-87	21.807392533065595	27.739283922495222	28.104182798367294	22.349140746071892
88-89	21.47902502965435	27.703668831318684	28.216323660861953	22.600982478165015
90-91	21.481669063118954	27.50879015416578	28.3032194718492	22.706321310866066
92-93	21.57329812041348	27.68486590613037	28.145550838037646	22.59628513541851
94-95	21.379823350856707	27.45383383411104	28.31198681823846	22.854355996793792
96-97	21.331730229280804	27.436972010295555	28.336448770671502	22.894848989752134
98-99	21.351269021475748	27.525527355078317	28.209585085286058	22.913618538159877
100	21.488548267739915	27.36809392158944	28.09447544496907	23.048882365701573
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	38.0
1	32.5
2	102.0
3	190.0
4	145.5
5	119.5
6	265.0
7	600.5
8	1071.0
9	1287.5
10	1123.5
11	987.0
12	1036.5
13	1163.5
14	1333.0
15	1528.0
16	1769.5
17	2084.0
18	2412.5
19	2869.5
20	3470.5
21	4252.5
22	5658.0
23	7775.0
24	10487.5
25	13910.0
26	18619.5
27	25166.5
28	33173.5
29	42179.5
30	51283.0
31	60444.0
32	69813.0
33	79058.5
34	88580.0
35	96971.5
36	103313.0
37	109730.5
38	114833.5
39	116736.5
40	118604.0
41	119921.5
42	118310.5
43	116436.0
44	114679.0
45	112052.0
46	110634.5
47	108272.0
48	105287.0
49	103393.5
50	101240.5
51	98636.0
52	93002.5
53	87391.5
54	83718.0
55	79445.5
56	73316.0
57	66715.0
58	60633.5
59	54597.0
60	48660.5
61	41161.0
62	34587.0
63	28914.5
64	23199.5
65	18414.5
66	14175.5
67	10533.5
68	7894.0
69	6050.5
70	4264.5
71	2982.5
72	2244.5
73	1626.0
74	1182.0
75	848.5
76	537.0
77	353.0
78	222.5
79	108.0
80	51.0
81	22.5
82	12.0
83	5.5
84	4.5
85	4.5
86	2.5
87	0.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08935535832144852
2	9.538622961311654E-4
3	0.0
4	1.8461850892861266E-4
5	0.04064684171578289
6	9.230925446430633E-5
7	0.004246225705358091
8	3.3846726636912317E-4
9	1.230790059524084E-4
10-11	5.53855526785838E-4
12-13	0.0
14-15	6.15395029762042E-5
16-17	0.0
18-19	9.230925446430633E-5
20-21	0.0
22-23	2.000033846726637E-4
24-25	1.0769413020835736E-4
26-27	0.0014923329471729521
28-29	0.007969365635418446
30-31	0.03446212166667436
32-33	0.02392348178199939
34-35	0.026954302303577447
36-37	0.024492722184529275
38-39	0.02395425153348749
40-41	0.02993896819792335
42-43	0.02180036892932034
44-45	0.020215726727683084
46-47	0.014492552950896092
48-49	0.010323251624258257
50-51	0.007384740357144506
52-53	0.0051693182500011535
54-55	0.0039077584389889675
56-57	0.0024000406160719643
58-59	9.846320476192673E-4
60-61	6.461647812501442E-4
62-63	0.001400023692708646
64-65	0.002876971764137547
66-67	4.3077652083342946E-4
68-69	9.538622961311654E-4
70-71	3.0769751488102107E-4
72-73	4.4616139657748053E-4
74-75	5.077008995536847E-4
76-77	2.76927763392919E-4
78-79	7.692437872025527E-5
80-81	6.307799055060932E-4
82-83	5.230857752977358E-4
84-85	2.1538826041671473E-4
86-87	1.0769413020835736E-4
88-89	0.0
90-91	6.15395029762042E-5
92-93	4.6154627232153165E-5
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3249945.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.48916607835518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.2770185439115	47.039981136739094
2	15.029602259059738	18.783746233160127
3	4.644597720366364	8.70711114945371
4	1.9281228931173275	4.819471667499495
5	0.9417580602080746	2.9424837914985997
6	0.5679215073563496	2.129336483559645
7	0.34807098000026054	1.5225465693404492
8	0.2461869204250484	1.230721228540773
9	0.16860422009489848	0.9482343399919496
>10	0.7963611264478141	8.593766773851632
>50	0.03978083336087474	1.6751745549526207
>100	0.0114826298213367	1.1224043059342637
>500	2.953834999051901E-4	0.10567358752375723
>1k	1.969223305733585E-4	0.37934817795389186
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4842	0.1489871367053904	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4089	0.12581751383484951	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	3.07697514881021E-5	0.0
9	0.0	0.0	0.0	6.15395029762042E-5	0.0
10-11	0.0	0.0	0.0	9.230925446430632E-5	0.0
12-13	0.0	0.0	0.0	9.230925446430632E-5	0.0
14-15	0.0	0.0	0.0	9.230925446430632E-5	0.0
16-17	0.0	0.0	0.0	9.230925446430632E-5	0.0
18-19	0.0	0.0	0.0	9.230925446430632E-5	0.0
20-21	0.0	0.0	0.0	1.2307900595240844E-4	0.0
22-23	0.0	0.0	0.0	1.8461850892861263E-4	0.0
24-25	0.0	0.0	0.0	2.1538826041671475E-4	0.0
26-27	0.0	0.0	0.0	3.0769751488102107E-4	0.0
28-29	0.0	0.0	0.0	0.0012154051837800332	0.0
30-31	0.0	0.0	0.0	0.004430844214286704	0.0
32-33	0.0	0.0	0.0	0.012477134228425404	0.0
34-35	0.0	0.0	0.0	0.023292701876493294	0.0
36-37	0.0	0.0	0.0	0.04232379317188445	0.0
38-39	0.0	0.0	0.0	0.07501665412799294	0.0
40-41	0.0	0.0	0.0	0.11590965385568064	0.0
42-43	0.0	0.0	0.0	0.15554109377235614	0.0
44-45	0.0	0.0	0.0	0.20031108218754473	0.0
46-47	0.0	0.0	0.0	0.2549889305819022	0.0
48-49	0.0	0.0	0.0	0.318836164919714	0.0
50-51	0.0	0.0	0.0	0.3875757897441341	0.0
52-53	0.0	0.0	0.0	0.45553078590560764	0.0
54-55	0.0	0.0	0.0	0.5307935980455054	0.0
56-57	0.0	0.0	0.0	0.6189181663074298	0.0
58-59	0.0	0.0	0.0	0.721735290904923	0.0
60-61	0.0	0.0	0.0	0.8321371592442334	0.0
62-63	0.0	0.0	0.0	0.9433236562464904	0.0
64-65	0.0	0.0	0.0	1.0576332830247896	0.0
66-67	0.0	0.0	0.0	1.1917278600099386	0.0
68-69	0.0	0.0	0.0	1.3438227416156274	0.0
70-71	0.0	0.0	0.0	1.5111332653321825	0.0
72-73	0.0	0.0	0.0	1.6879670271343055	0.0
74-75	0.0	0.0	0.0	1.8647084796819637	0.0
76-77	0.0	0.0	0.0	2.0573732786247154	0.0
78-79	0.0	0.0	0.0	2.279007798593515	0.0
80-81	0.0	0.0	0.0	2.5209349696687173	0.0
82-83	0.0	0.0	0.0	2.7734930898830594	0.0
84-85	0.0	0.0	0.0	3.0236973241085616	0.0
86-87	0.0	0.0	0.0	3.2876556372492454	0.0
88	0.0	0.0	0.0	3.505597787039473	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4270	0.0	49.37177	1
GTATCAA	7725	0.0	41.970997	1
TCAACGC	8860	0.0	35.976307	4
ATCAACG	8870	0.0	35.935745	3
CAACGCA	9160	0.0	34.79804	5
AACGCAG	9575	0.0	33.326614	6
TATCAAC	9785	0.0	32.91221	2
ACGCAGA	11130	0.0	28.459345	7
CGCAGAG	11370	0.0	27.7751	8
GCAGAGT	12765	0.0	24.481665	9
GTACATG	8755	0.0	24.455908	1
TACATGG	8825	0.0	23.70678	2
ACATGGG	9100	0.0	21.853428	3
GAGTACT	8485	0.0	21.51655	12-13
CAGAGTA	12515	0.0	20.12646	10-11
AGAGTAC	11775	0.0	19.87476	10-11
GTGGTAT	2520	0.0	19.420542	1
TGGTATC	2370	0.0	19.24202	2
GTACTTT	9520	0.0	19.078579	14-15
GAGTACA	6900	0.0	18.891184	1
>>END_MODULE