FastQCFastQC Report
Wed 25 May 2016
SRR1033169_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033169_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2559377
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG35020.13683017390560281No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA33340.13026607647095367No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA33100.12932834826600378No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG33060.12917206023184546No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC32880.1284687640781331No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT32350.12639794762553544No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT31410.12272517882281508No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA29860.11666901749918046No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA29460.11510613715759735No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28730.11225388053420814No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC28660.11198037647443108No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG28260.11041749613284796No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC27220.10635400724473183No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA26140.10213423032245739No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTAG3800.049.5975881
TAGCGCA500.001709671137.5674639
GACTCGT801.1660988E-535.21957
TACATGG93400.031.6108172
GTACACG600.00411128731.4118021
AATACCG753.2876726E-431.31545
GTACATG111900.030.3591291
CATGGGT11150.029.9111254
ACTCGTC1102.990424E-629.8832138
TAGATGG11550.027.6470537
ACATGGG103300.027.513563
GTTGTCG2750.027.3298055
GATGACC6750.027.132069
ACGCAGT3350.026.6336528
ATGGGAT24850.025.896645
CATGGGA48700.025.6567654
ATACGCT1659.378891E-825.621694
CATACGC1501.168286E-625.054773
GGCCCGC1501.1722732E-625.0449778
CATGGGG31900.025.0326884
Produced by FastQC (version 0.11.5)