FastQCFastQC Report
Wed 25 May 2016
SRR1033169_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033169_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2559377
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71360.27881785293843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64150.25064693478139405No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG39530.15445164975695255No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA36680.14331612732317278No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35490.138666558306963No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG34000.13284482903456585No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA33280.1300316444197162No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT33010.1289767001891476No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC32680.1276873239073415No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT32140.12557743544620428No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA31590.12342847497652748No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA30540.11932591407987178No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC28090.10975327198767514No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG27030.10561163908247985No Hit
GTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGT25970.10147000617728454No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTAG4150.044.2214241
GGTATCA71500.036.855151
CGTTCGG300.00415453831.34530424-25
TACATGG98600.031.1829992
GTATCAA121900.030.9975951
GTACATG112650.030.2429081
ATGGGAT23150.029.6501835
TACACGG650.006147311528.9310172
CATGGGT15150.028.8588394
ACATGGG107700.028.0238553
GATGACC6750.027.846989
CATGGGA49650.027.1751044
AGCGCGG350.00881859126.86976426-27
ATTGCGA350.00883920726.85690148-49
CTCGCGA350.00884215526.85506660-61
ATCAACG139350.026.7194063
CAACGCA139900.026.682615
TCAACGC140200.026.5909444
ACGCAGT3200.026.4344237
TAGATGG12500.025.5650257
Produced by FastQC (version 0.11.5)