FastQCFastQC Report
Wed 25 May 2016
SRR1033168_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033168_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3332364
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54550.027.2920861
GTACATG92000.026.526891
TCAACGC53200.026.1382984
ATCAACG53650.025.922173
TACATGG92000.025.1908782
CAACGCA55250.025.0830525
ACATGGG88950.024.6672233
GGTATCA37850.024.3968961
AACGCAG58950.023.339176
TATCAAC64350.022.281042
TAGACCG1554.2572123E-521.2156855
CGCAGAG65000.021.0223488
ACGCAGA65750.020.9253867
GTACCGT2601.7034836E-819.8664846
CATGGGG58200.019.3724924
GAGTACA63200.018.8602681
ACGTGCG2001.4198766E-518.7828569
GTACGAC2603.6838537E-718.0707453
GTATAGA14500.017.2207581
GTACTAG7450.017.0746541
Produced by FastQC (version 0.11.5)