FastQCFastQC Report
Wed 25 May 2016
SRR1033168_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033168_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3332364
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56370.16915919149288614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47940.1438618350216243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54250.041.7229841
GTATCAA87900.037.4212531
TCAACGC101750.031.3238624
ATCAACG102400.031.2168453
CAACGCA104600.030.6047575
AACGCAG108600.029.5137086
TATCAAC110350.029.2660712
ACGCAGA123250.025.8911937
CGCAGAG124450.025.6777618
GTACATG91700.025.1452961
TATACCG2101.4297257E-924.6233355
TACATGG89850.024.2238372
TATAACG2653.6379788E-1123.0609652
ACATGGG90200.022.7227023
GCAGAGT141050.022.4558759
AGAGTAC117800.020.50505810-11
GAGTACT86800.020.46516412-13
CATGGGG61600.019.6887914
CAGAGTA141000.018.4643410-11
GAGTACA64800.018.2275681
Produced by FastQC (version 0.11.5)