##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033168_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3332364 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.740356095552585 34.0 31.0 34.0 31.0 34.0 2 32.83611694280697 34.0 31.0 34.0 31.0 34.0 3 32.942277014155714 34.0 31.0 34.0 31.0 34.0 4 36.37276900122556 37.0 37.0 37.0 35.0 37.0 5 36.301272910162275 37.0 37.0 37.0 35.0 37.0 6 36.386959827917956 37.0 37.0 37.0 35.0 37.0 7 36.36323462863 37.0 37.0 37.0 35.0 37.0 8 36.357581284637575 37.0 37.0 37.0 35.0 37.0 9 38.17110255662347 39.0 39.0 39.0 37.0 39.0 10-11 38.15777778177894 39.0 39.0 39.0 37.0 39.0 12-13 38.1184076829542 39.0 39.0 39.0 37.0 39.0 14-15 39.66089343781172 41.0 40.0 41.0 37.0 41.0 16-17 39.67049337947475 41.0 40.0 41.0 37.0 41.0 18-19 39.683060884105096 41.0 40.0 41.0 37.0 41.0 20-21 39.67327218755214 41.0 40.0 41.0 37.0 41.0 22-23 39.60565622483018 41.0 40.0 41.0 37.0 41.0 24-25 39.559577075013415 41.0 40.0 41.0 37.0 41.0 26-27 39.46036582438173 41.0 39.0 41.0 36.5 41.0 28-29 39.37326549560612 41.0 39.0 41.0 36.0 41.0 30-31 39.28682910990516 41.0 39.0 41.0 36.0 41.0 32-33 39.33402023308378 41.0 39.0 41.0 36.0 41.0 34-35 39.281029323327225 41.0 39.0 41.0 35.5 41.0 36-37 39.206444434041416 41.0 39.0 41.0 35.0 41.0 38-39 39.125006601919836 41.0 39.0 41.0 35.0 41.0 40-41 39.0220008978611 41.0 39.0 41.0 35.0 41.0 42-43 38.916181725645814 41.0 39.0 41.0 35.0 41.0 44-45 38.78314148754458 41.0 39.0 41.0 35.0 41.0 46-47 38.589735395052884 41.0 39.0 41.0 35.0 41.0 48-49 38.4171226492664 41.0 39.0 41.0 35.0 41.0 50-51 38.29340897332945 41.0 38.0 41.0 34.5 41.0 52-53 38.16643079807608 40.0 38.0 41.0 34.0 41.0 54-55 38.03277673147352 40.0 38.0 41.0 34.0 41.0 56-57 37.87454671818565 40.0 37.0 41.0 34.0 41.0 58-59 37.69265452393556 40.0 37.0 41.0 34.0 41.0 60-61 37.49741954960503 40.0 36.5 41.0 33.0 41.0 62-63 37.2684637692641 39.5 36.0 41.0 33.0 41.0 64-65 37.0220225041442 39.0 35.5 41.0 33.0 41.0 66-67 36.741237451851 39.0 35.0 41.0 33.0 41.0 68-69 36.41836050923608 38.5 35.0 40.5 32.0 41.0 70-71 36.08292086338707 37.0 35.0 40.0 32.0 41.0 72-73 35.730102113694656 37.0 35.0 39.5 31.5 41.0 74-75 35.39422644104906 36.0 35.0 39.0 31.0 41.0 76-77 35.052734485188296 36.0 35.0 39.0 31.0 40.5 78-79 34.706354107774544 35.5 35.0 37.5 31.0 39.5 80-81 34.36527237120555 35.0 35.0 37.0 31.0 39.0 82-83 34.03889971203626 35.0 34.5 37.0 31.0 39.0 84-85 33.76466016317545 35.0 34.0 36.0 30.0 38.0 86-87 33.527992440201615 35.0 34.0 36.0 30.0 37.0 88-89 33.30603109384209 35.0 34.0 36.0 30.0 37.0 90-91 33.105741299569914 35.0 34.0 35.0 30.0 36.5 92-93 32.94250087925569 35.0 34.0 35.0 30.0 36.0 94-95 32.79426407199213 35.0 34.0 35.0 30.0 36.0 96-97 32.67252677078494 35.0 34.0 35.0 29.5 36.0 98-99 32.566493486305816 35.0 34.0 35.0 29.5 36.0 100 32.471158913011905 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11556937762972552 1101 2 0.11514389403937741 1101 3 0.13469117601285774 1101 4 0.09683395857472732 1101 5 0.06255353762237803 1101 6 0.11254415216396296 1101 7 0.12535082303239875 1101 8 0.14565739145514556 1101 9 0.16303131070760202 1101 10-11 0.17379640199458635 1101 12-13 0.17938025840759053 1101 14-15 0.21239663962139588 1101 16-17 0.21752238316865657 1101 18-19 0.18450805551602656 1101 20-21 0.19912318921208794 1101 22-23 0.24683649340680347 1101 24-25 0.15372845960767734 1101 26-27 0.15158142622454562 1101 28-29 0.1578531202441269 1101 30-31 0.17215966936507243 1101 32-33 0.17077688673694524 1101 34-35 0.11443923202387651 1101 36-37 0.17027475569426542 1101 38-39 0.10315226537667144 1101 40-41 0.15280913331190504 1101 42-43 0.08099543770980944 1101 44-45 0.08873436220157771 1101 46-47 0.10610187416968131 1101 48-49 0.13532276949851507 1101 50-51 0.13169284093402212 1101 52-53 0.19067507836482633 1101 54-55 0.20238512325795455 1101 56-57 0.1306912410858203 1101 58-59 0.10200299282986691 1101 60-61 0.16846316407026762 1101 62-63 0.1410663223178119 1101 64-65 0.18559511605123902 1101 66-67 0.15586428359844717 1101 68-69 0.17221908583675827 1101 70-71 0.1706638541774126 1101 72-73 0.1942252924854131 1101 74-75 0.21863876348738387 1101 76-77 0.15389564571145087 1101 78-79 0.1238946345935048 1101 80-81 0.11303272881370674 1101 82-83 0.11138607154877178 1101 84-85 0.13959547890894441 1101 86-87 0.072679323396585 1101 88-89 0.10504584921800841 1101 90-91 0.11474013769603886 1101 92-93 0.09455893222158807 1101 94-95 0.17390886022626262 1101 96-97 0.24274001705462211 1101 98-99 0.27237223998824334 1101 100 0.27952624138791293 1102 1 0.04733466259705921 1102 2 0.05478219410664309 1102 3 0.05653109774817722 1102 4 0.05974313388867358 1102 5 -3.051786804419976E-4 1102 6 0.03566918236458605 1102 7 0.0831627561714754 1102 8 0.11533692352379887 1102 9 0.137078140658339 1102 10-11 0.09292869395925862 1102 12-13 0.10152508660343074 1102 14-15 0.12594505189309047 1102 16-17 0.14669281133106438 1102 18-19 0.09103168275863283 1102 20-21 0.08131704979909671 1102 22-23 0.1251403937550961 1102 24-25 0.1405004424023346 1102 26-27 0.12254570722726754 1102 28-29 0.14961657112276328 1102 30-31 0.14826541488521627 1102 32-33 0.11225802409821029 1102 34-35 0.18774065304473453 1102 36-37 0.13240446727925814 1102 38-39 0.15026082885474068 1102 40-41 0.11899649050609185 1102 42-43 0.11056108178653545 1102 44-45 0.1854841470580837 1102 46-47 0.17350264450610808 1102 48-49 0.20310600916836563 1102 50-51 0.18876312823687869 1102 52-53 0.2074853210359464 1102 54-55 0.2117673306608836 1102 56-57 0.2672615795859201 1102 58-59 0.2570775893303505 1102 60-61 0.24432880983439276 1102 62-63 0.27995415125533896 1102 64-65 0.24876250190822446 1102 66-67 0.18535596011467703 1102 68-69 0.25361698260776677 1102 70-71 0.2669050461414031 1102 72-73 0.2519978881130598 1102 74-75 0.2466427476687727 1102 76-77 0.24322774979948036 1102 78-79 0.16942897855158634 1102 80-81 0.14429957884451028 1102 82-83 0.09856535530117583 1102 84-85 0.15201378382364794 1102 86-87 0.14473602329480428 1102 88-89 0.14960682226486455 1102 90-91 0.18444706125145416 1102 92-93 0.14184575128859933 1102 94-95 0.1615831226215576 1102 96-97 0.16793189648068818 1102 98-99 0.1779015476327075 1102 100 0.1583656994717444 1103 1 0.13199116529946053 1103 2 0.14545354576896585 1103 3 0.12473854621207181 1103 4 0.08779894612820272 1103 5 0.09185361830191141 1103 6 0.10107793831150502 1103 7 0.0964837300202035 1103 8 0.15660961973103582 1103 9 0.14014607716019611 1103 10-11 0.12452169847502148 1103 12-13 0.13082362710407835 1103 14-15 0.21412648340874085 1103 16-17 0.2272101076686468 1103 18-19 0.17129694537459983 1103 20-21 0.16922590111572333 1103 22-23 0.16876822705796002 1103 24-25 0.13812119253144317 1103 26-27 0.20965559277947676 1103 28-29 0.23910312960805413 1103 30-31 0.24818074867297923 1103 32-33 0.2210590076049357 1103 34-35 0.22362478821451504 1103 36-37 0.22293767666801756 1103 38-39 0.21549439904700307 1103 40-41 0.29727730853107204 1103 42-43 0.2311780154247245 1103 44-45 0.2526479817237828 1103 46-47 0.1663871900072138 1103 48-49 0.10729842929904976 1103 50-51 0.1426280034654539 1103 52-53 0.09788134529947001 1103 54-55 0.05455544116678368 1103 56-57 0.05849462086170121 1103 58-59 0.07995915432677236 1103 60-61 0.09122023364269438 1103 62-63 0.00513549059944296 1103 64-65 0.025740498402569756 1103 66-67 0.038890614567336 1103 68-69 0.06977524782059419 1103 70-71 0.10458028193926339 1103 72-73 0.07733842699401094 1103 74-75 0.11654295897307776 1103 76-77 0.0341711846799555 1103 78-79 0.06001392456864352 1103 80-81 0.08450077050338223 1103 82-83 0.10906403004188547 1103 84-85 0.08836790643407255 1103 86-87 0.09466128979733668 1103 88-89 0.10692368976318534 1103 90-91 0.012636842045615992 1103 92-93 0.0430168958985675 1103 94-95 0.10060195999096777 1103 96-97 0.19082665447838565 1103 98-99 0.1810588256482646 1103 100 0.17853341302706838 1104 1 0.09138486183449146 1104 2 0.11230006191035358 1104 3 0.06249774692235377 1104 4 0.04977715395580162 1104 5 0.019526015765400473 1104 6 0.04695558658127652 1104 7 0.07470496189400677 1104 8 0.07760311795338026 1104 9 0.11276026657032645 1104 10-11 0.10408503313349371 1104 12-13 0.09858386047245915 1104 14-15 0.11872472649663024 1104 16-17 0.19432709028224338 1104 18-19 0.1363347004482094 1104 20-21 0.13871830600800195 1104 22-23 0.16586095499320663 1104 24-25 0.14023798730885773 1104 26-27 0.08317640485262956 1104 28-29 0.08605343273861621 1104 30-31 0.11471418686534918 1104 32-33 0.06581471385665338 1104 34-35 0.131753205201683 1104 36-37 0.07829025400978651 1104 38-39 0.08422327188526424 1104 40-41 0.13437346513812543 1104 42-43 0.12153704092705908 1104 44-45 0.12027686644950109 1104 46-47 0.09857301326059087 1104 48-49 0.08711505620559024 1104 50-51 0.07588497800827554 1104 52-53 0.03889624097366351 1104 54-55 0.014657844517572016 1104 56-57 0.06523275905058057 1104 58-59 0.11076746370585511 1104 60-61 0.14836782826363049 1104 62-63 0.12120028632361368 1104 64-65 0.1545521840709725 1104 66-67 0.15868680260388146 1104 68-69 0.17530342554149314 1104 70-71 0.16907970114624504 1104 72-73 0.19890719779046862 1104 74-75 0.25645816636714613 1104 76-77 0.17576805506453042 1104 78-79 0.15160683981095957 1104 80-81 0.2058763698595527 1104 82-83 0.18676489394724882 1104 84-85 0.17963585625412293 1104 86-87 0.135846093444151 1104 88-89 0.12751465856403144 1104 90-91 0.15532951170754217 1104 92-93 0.1317927754040369 1104 94-95 0.12524732438787822 1104 96-97 0.13442506935260923 1104 98-99 0.1543781944340168 1104 100 0.1709854848161214 1105 1 0.02442744498151228 1105 2 -5.93138456736142E-4 1105 3 0.02313652135993749 1105 4 0.02265106023856589 1105 5 -0.0020620494069376605 1105 6 -0.0022310527078488462 1105 7 -0.06764507663969255 1105 8 -0.054507575898384175 1105 9 -0.009762955773233273 1105 10-11 -0.0017515975774600179 1105 12-13 -0.013942214454488067 1105 14-15 0.009439805837935467 1105 16-17 0.003990505866205751 1105 18-19 0.038098264175864927 1105 20-21 0.03943648983147341 1105 22-23 0.07471019289729952 1105 24-25 -0.014036905055476723 1105 26-27 0.026522328585855348 1105 28-29 0.011330555428301636 1105 30-31 -0.008334278458406175 1105 32-33 -0.01984805904503162 1105 34-35 0.047137684424797044 1105 36-37 -0.02451286871801983 1105 38-39 -0.0051185847040073895 1105 40-41 0.04619970400352713 1105 42-43 0.00951765697615059 1105 44-45 0.13466045653804315 1105 46-47 0.14826668392950637 1105 48-49 0.07301144819820138 1105 50-51 0.009179459927388223 1105 52-53 0.02052737402795657 1105 54-55 0.04882144528116328 1105 56-57 0.038920740892081085 1105 58-59 0.05193156962860712 1105 60-61 -0.024518638917363944 1105 62-63 0.0018290324668441826 1105 64-65 -0.030847184412003514 1105 66-67 -0.0677405783841607 1105 68-69 -0.07917016048942571 1105 70-71 -0.010086364140470039 1105 72-73 -0.026984217499375518 1105 74-75 -0.05218420818045644 1105 76-77 -0.07554488218408295 1105 78-79 -0.052626216932985415 1105 80-81 -0.023028469592418332 1105 82-83 -0.016951770313895054 1105 84-85 -0.09305511705550629 1105 86-87 -0.12105014604758679 1105 88-89 -0.07511050108532658 1105 90-91 -0.08318237246803761 1105 92-93 -0.02454666568024777 1105 94-95 -0.045350608408249116 1105 96-97 -0.051625321294729076 1105 98-99 -0.06997748935540926 1105 100 -0.05820393216757935 1106 1 -0.04622721309220168 1106 2 0.010427399622081168 1106 3 0.021916798470464016 1106 4 0.024736713457429005 1106 5 0.0019396128972175575 1106 6 -0.002193549895139313 1106 7 -0.026035545668463556 1106 8 0.031344776489589776 1106 9 0.013660515730720135 1106 10-11 0.029138459561444563 1106 12-13 0.03444074119026652 1106 14-15 0.026561001786610916 1106 16-17 0.004849424451762729 1106 18-19 0.009083548008057107 1106 20-21 -0.01990457933845846 1106 22-23 0.004708909147169038 1106 24-25 -0.026437274185731496 1106 26-27 -0.030972848937075526 1106 28-29 -0.03186699528544068 1106 30-31 -0.02485530920237977 1106 32-33 -0.0014087754577403189 1106 34-35 0.014658125060407201 1106 36-37 0.06440341538790761 1106 38-39 0.012385011719572958 1106 40-41 0.01517542205417044 1106 42-43 0.058777240868018055 1106 44-45 0.13185756202607735 1106 46-47 0.13767895074694536 1106 48-49 0.10763587996648027 1106 50-51 0.05009458785685439 1106 52-53 0.07693841575352423 1106 54-55 0.051055026261281 1106 56-57 0.06391147737680569 1106 58-59 0.0292585149828426 1106 60-61 0.060619156673716645 1106 62-63 0.019775410496478685 1106 64-65 -0.00949053458634097 1106 66-67 0.060896072569789794 1106 68-69 0.03551608520977112 1106 70-71 0.04695477291916461 1106 72-73 0.06459007845743514 1106 74-75 0.060644686678969606 1106 76-77 0.006294749378021436 1106 78-79 0.03922245857665274 1106 80-81 0.09299377374249218 1106 82-83 0.054149876412168396 1106 84-85 -0.00974971049616613 1106 86-87 0.022882844439514827 1106 88-89 -0.02472699475824669 1106 90-91 0.005654779100382257 1106 92-93 0.008969670160325904 1106 94-95 -0.05636265226025472 1106 96-97 0.002934019053235204 1106 98-99 -0.009193763909337349 1106 100 0.018329772603870254 1107 1 -0.048867017782271205 1107 2 -0.06387686193991016 1107 3 -0.054756642124829114 1107 4 -0.03427054945525754 1107 5 -0.12109746455229242 1107 6 -0.16282307890473646 1107 7 -0.12588377019686448 1107 8 -0.03871670228978985 1107 9 -0.09675294060487971 1107 10-11 -0.08725510684838156 1107 12-13 -0.041196682256135375 1107 14-15 -0.05034243785353709 1107 16-17 -0.03364527323574862 1107 18-19 -0.03484237649598043 1107 20-21 -0.041701488986959134 1107 22-23 -0.05361174860615847 1107 24-25 -0.05303995740769807 1107 26-27 -0.03369172904871931 1107 28-29 -0.07578602020358005 1107 30-31 -0.0531042182005379 1107 32-33 -0.048308884024066856 1107 34-35 -0.04460475016431786 1107 36-37 -0.030232807993087363 1107 38-39 -0.011056736078899121 1107 40-41 -0.00944709928887022 1107 42-43 -0.00925009351822581 1107 44-45 0.001169672541564637 1107 46-47 -0.04030484840865256 1107 48-49 3.2843800426007874E-6 1107 50-51 -0.052924420386645465 1107 52-53 0.00500562666260862 1107 54-55 -0.012126428299396252 1107 56-57 -0.07591044238402134 1107 58-59 -0.013230098580450544 1107 60-61 -0.009830240722614292 1107 62-63 0.011956618599320734 1107 64-65 -0.02743676222040392 1107 66-67 -0.01367378450394341 1107 68-69 -0.017605599664477722 1107 70-71 -0.010268600133422012 1107 72-73 9.194007363788614E-5 1107 74-75 -0.03335877914877017 1107 76-77 -0.05120747872567932 1107 78-79 -0.10894593649409501 1107 80-81 -0.09109546511600541 1107 82-83 -0.03395053114135749 1107 84-85 -0.07011168204473961 1107 86-87 -0.066514434718421 1107 88-89 -0.04009352889890039 1107 90-91 -0.05708064999566176 1107 92-93 -0.06280130629605196 1107 94-95 -0.10920326573597805 1107 96-97 -0.07943811331119122 1107 98-99 -0.09154930257696492 1107 100 -0.10156140203945085 1108 1 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-0.07799334152612403 2214 12-13 -0.08046071010224409 2214 14-15 -0.10607472974331955 2214 16-17 -0.12168615062616084 2214 18-19 -0.10698486109880889 2214 20-21 -0.10643595752603119 2214 22-23 -0.15224089633241533 2214 24-25 -0.1350591360119111 2214 26-27 -0.21687075191213268 2214 28-29 -0.12827619435862658 2214 30-31 -0.09922054240148981 2214 32-33 -0.1346820679418883 2214 34-35 -0.2053793917225235 2214 36-37 -0.24756694332786822 2214 38-39 -0.20103005291439047 2214 40-41 -0.12392046402588619 2214 42-43 -0.19029564567659918 2214 44-45 -0.1790047579465721 2214 46-47 -0.17711931755722787 2214 48-49 -0.2516188087051461 2214 50-51 -0.19286167577043045 2214 52-53 -0.2310856001744881 2214 54-55 -0.2337054762046762 2214 56-57 -0.2564499862367384 2214 58-59 -0.3525951109019516 2214 60-61 -0.33767551442819155 2214 62-63 -0.38256790130901663 2214 64-65 -0.2803195840658006 2214 66-67 -0.36135416665707965 2214 68-69 -0.23380952452799875 2214 70-71 -0.2064876445720074 2214 72-73 -0.3090783762615317 2214 74-75 -0.28534683020975393 2214 76-77 -0.28012459509610466 2214 78-79 -0.3825768053311762 2214 80-81 -0.34445218280373524 2214 82-83 -0.3251682740168178 2214 84-85 -0.3642839266790361 2214 86-87 -0.2815926996690976 2214 88-89 -0.40877570338599156 2214 90-91 -0.23986629087406897 2214 92-93 -0.29745524615400143 2214 94-95 -0.3186128276795728 2214 96-97 -0.4521034226014464 2214 98-99 -0.5154965093425119 2214 100 -0.643350127955145 2215 1 -0.06897497255535256 2215 2 -0.055460380365111916 2215 3 -0.1286186656197188 2215 4 -0.10643506825346094 2215 5 -0.1187044757159228 2215 6 -0.12973971643872773 2215 7 -0.1327525001892127 2215 8 -0.13777593183289127 2215 9 -0.14027539353129015 2215 10-11 -0.15307104553786388 2215 12-13 -0.12318193762909146 2215 14-15 -0.19700043574791692 2215 16-17 -0.18858273129637837 2215 18-19 -0.1776040015271647 2215 20-21 -0.22117736721480696 2215 22-23 -0.2015887478463867 2215 24-25 -0.1857581839729292 2215 26-27 -0.1816007810089033 2215 28-29 -0.19699322069121905 2215 30-31 -0.21629700168811183 2215 32-33 -0.24286772974913617 2215 34-35 -0.2055577121124017 2215 36-37 -0.1676372272774529 2215 38-39 -0.2199677292121578 2215 40-41 -0.26349668149551064 2215 42-43 -0.2034211520066691 2215 44-45 -0.2489405988301172 2215 46-47 -0.26801180833936655 2215 48-49 -0.2597098213485083 2215 50-51 -0.22250846967519777 2215 52-53 -0.2616332915009778 2215 54-55 -0.2290263060649309 2215 56-57 -0.3460409761728087 2215 58-59 -0.29619654631447645 2215 60-61 -0.31615217002441653 2215 62-63 -0.2882278380836283 2215 64-65 -0.1951499907922667 2215 66-67 -0.2595097683137908 2215 68-69 -0.2562705889975945 2215 70-71 -0.23243193658167627 2215 72-73 -0.15639638101668396 2215 74-75 -0.2236106587067681 2215 76-77 -0.2743528551941452 2215 78-79 -0.2376294064782698 2215 80-81 -0.2579460323294711 2215 82-83 -0.12954222934268245 2215 84-85 -0.16197403323388926 2215 86-87 -0.2786584738027713 2215 88-89 -0.2725811518141015 2215 90-91 -0.2513252731877955 2215 92-93 -0.2674931097113813 2215 94-95 -0.2009023752666863 2215 96-97 -0.16866441277013422 2215 98-99 -0.26643781437713443 2215 100 -0.2504143157214074 2216 1 -0.05628265094834006 2216 2 -0.07292471276843315 2216 3 -0.13878202677929607 2216 4 -0.09181761675922928 2216 5 -0.06283422877284295 2216 6 -0.04199936794596226 2216 7 -0.05884788687496467 2216 8 -0.052671490722488556 2216 9 -0.09367238769866049 2216 10-11 -0.11589242646436304 2216 12-13 -0.10248509725890642 2216 14-15 -0.19926076715639596 2216 16-17 -0.13798788575304854 2216 18-19 -0.12137771372105988 2216 20-21 -0.15182833324729472 2216 22-23 -0.15604267940511818 2216 24-25 -0.1955009023099663 2216 26-27 -0.2511978740256353 2216 28-29 -0.2652434684667355 2216 30-31 -0.2147945560283233 2216 32-33 -0.15517059099359187 2216 34-35 -0.2159010996673345 2216 36-37 -0.26411607883295574 2216 38-39 -0.28050726459066766 2216 40-41 -0.3121093654019944 2216 42-43 -0.23660810148654576 2216 44-45 -0.13975024496731692 2216 46-47 -0.19358183096606751 2216 48-49 -0.17699595044446426 2216 50-51 -0.09433942955399033 2216 52-53 -0.0769385433439993 2216 54-55 -0.0739083253364754 2216 56-57 -0.04010490809785239 2216 58-59 -0.10545188206491218 2216 60-61 -0.09606283474327881 2216 62-63 -0.061952833283498876 2216 64-65 -0.017381247491336183 2216 66-67 -0.0185029508152752 2216 68-69 0.008586429868501 2216 70-71 2.1824815979698542E-4 2216 72-73 0.05561375410503899 2216 74-75 0.01764431707593417 2216 76-77 0.05232429786710924 2216 78-79 0.03507722537715807 2216 80-81 2.0706952291504876E-4 2216 82-83 0.007609687020504907 2216 84-85 -0.015027470296303136 2216 86-87 -4.85937775657419E-4 2216 88-89 -0.0026213153215621787 2216 90-91 0.03459662016535958 2216 92-93 0.00882833988571008 2216 94-95 9.795562244434564E-4 2216 96-97 0.025380905511546814 2216 98-99 -0.008058995518140932 2216 100 -0.0021212704590425346 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15.0 3 3.0 4 15.0 5 19.0 6 60.0 7 145.0 8 386.0 9 652.0 10 1195.0 11 1903.0 12 2915.0 13 4669.0 14 5365.0 15 7156.0 16 9006.0 17 13214.0 18 15467.0 19 8552.0 20 5841.0 21 5776.0 22 6049.0 23 6474.0 24 7166.0 25 8886.0 26 11420.0 27 14651.0 28 18778.0 29 24359.0 30 30942.0 31 39686.0 32 51260.0 33 67151.0 34 94187.0 35 144976.0 36 270202.0 37 637071.0 38 1197735.0 39 616407.0 40 2610.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.61825488720788 20.119812996811696 13.54208247543443 23.71984964054599 2 15.379382263134596 20.356897961633393 40.319056514595665 23.944663260636343 3 17.952840686071507 25.835773042800845 30.503180324838464 25.70820594628918 4 12.038446060851223 16.9335698426069 39.211254976582644 31.816729119959238 5 12.354604431894971 38.22676770420972 36.16322908670855 13.25539877718676 6 30.50232582984006 38.83273080721495 17.62136072373955 13.04358263920544 7 26.73251103399825 32.91359074677668 23.050710326297843 17.303187892927227 8 25.11007994055848 36.664054896978136 20.24951753281692 17.976347629646458 9 25.078998114848407 16.235200419642787 21.37921555847241 37.3065859070364 10-11 23.81611289813882 26.793337072437488 29.23289247314732 20.15765755627637 12-13 24.658260622188934 24.102904124519412 29.476296707082422 21.762538546209235 14-15 21.619574944138723 26.02340321969822 26.838680791582668 25.51834104458039 16-17 21.030070544514345 29.577996281318608 27.358835949494114 22.033097224672936 18-19 20.959568906249952 28.257892827337734 29.86720226290009 20.915336003512223 20-21 21.991265059879414 27.47683026224026 29.546172026825403 20.985732651054928 22-23 21.517165922748998 27.298252468672334 29.664549847285315 21.520031761293353 24-25 21.451532412004582 27.456299002419005 29.58492492259992 21.507243662976492 26-27 21.23557517086077 27.430451200868106 29.676154905360935 21.65781872291019 28-29 21.291994402296943 27.546659279891454 29.739132992265194 21.42221332554641 30-31 21.479236012046282 27.847033098459633 29.406703746931996 21.267027142562092 32-33 21.23278748557338 27.545786226386493 29.764386472820924 21.457039815219204 34-35 21.337963198515226 27.41532162989625 29.73624266809189 21.51047250349664 36-37 21.667055630304947 27.621426068267997 29.271972630967557 21.439545670459502 38-39 21.466794976120575 27.87916599255177 29.32446333200161 21.329575699326046 40-41 21.400858336117505 27.59284657864774 29.49414482928585 21.512150255948903 42-43 21.397252379480193 27.583105465525676 29.313094870047813 21.706547284946318 44-45 21.468664301764942 27.74368295249231 29.170758120739055 21.616894625003695 46-47 21.669748516825223 27.885936828924752 28.899060678483245 21.545253975766784 48-49 21.667186122033346 27.830662852558756 28.71439223497009 21.7877587904378 50-51 21.649003431609913 27.892465718788657 28.610569370544397 21.847961479057034 52-53 21.69620585177769 27.946070805213637 28.529742055060588 21.827981287948084 54-55 21.72051252195228 28.23925913833027 28.384372257840703 21.655856081876752 56-57 21.559888520872928 27.919042290225647 28.56040438342122 21.960664805480206 58-59 21.522557462727328 27.610617663175418 28.825141777504896 22.041683096592358 60-61 21.682433841634044 27.94976210446397 28.59108225588887 21.77672179801311 62-63 21.481608297887536 27.942216199054233 28.600526720439046 21.975648782619185 64-65 21.513828727958693 27.796580465064913 28.641350960577576 22.04823984639882 66-67 21.446853255766428 27.81072750783306 28.582375375448372 22.16004386095214 68-69 21.684985675192532 27.9064114579254 28.5645543614965 21.84404850538557 70-71 21.718487633893695 27.73366399043079 28.5332337841007 22.014614591574816 72-73 21.57874867810925 27.859230188443707 28.582594014790775 21.979427118656275 74-75 21.553381480543983 27.951119773000997 28.41250595113148 22.082992795323538 76-77 21.69006206128946 27.820613734867667 28.34276761361573 22.14655659022714 78-79 21.677953704016296 27.97882883389787 28.41140482165431 21.931812640431524 80-81 21.665994217588118 27.727593754130908 28.516227229071166 22.090184799209805 82-83 21.505539940930504 27.880433184309194 28.539293338686917 22.074733536073385 84-85 21.436176517807976 27.736975802398007 28.44266292156748 22.38418475822654 86-87 21.6170274443514 27.850159714064638 28.31931649672462 22.213496344859344 88-89 21.38913996190092 27.770255590325664 28.426456413525052 22.41414803424836 90-91 21.338911757212525 27.720314383517042 28.490608616168167 22.450165243102266 92-93 21.409924217451067 27.84327099969736 28.42574046918951 22.32106431366206 94-95 21.291371530841168 27.6111493222229 28.48449329064892 22.612985856287008 96-97 21.214804265080286 27.580885521509657 28.541464857980703 22.662845355429358 98-99 21.13657151499656 27.737200978044413 28.408871299773974 22.71735620718505 100 21.250589671476465 27.547170717244573 28.35737632503532 22.844863286243637 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 75.0 1 70.0 2 160.5 3 287.0 4 214.5 5 142.0 6 361.5 7 863.0 8 1485.0 9 1754.5 10 1455.5 11 1141.0 12 1200.5 13 1381.5 14 1569.0 15 1828.0 16 2086.5 17 2345.0 18 2678.0 19 3130.5 20 3820.5 21 4820.0 22 6411.0 23 9167.0 24 12814.5 25 17034.0 26 22871.5 27 30445.5 28 39720.0 29 49827.5 30 58704.5 31 67272.0 32 75905.5 33 84168.5 34 92122.5 35 99272.5 36 105311.0 37 111040.5 38 115424.0 39 116607.5 40 118115.0 41 119682.5 42 119521.5 43 119654.0 44 117989.5 45 115676.5 46 114065.0 47 110449.5 48 107226.0 49 104923.5 50 101625.5 51 96967.0 52 89713.5 53 84510.5 54 83102.5 55 79897.5 56 73520.0 57 66936.5 58 60236.5 59 52813.0 60 46703.0 61 40436.5 62 34119.0 63 28550.0 64 23179.5 65 18701.0 66 14556.5 67 10904.5 68 8397.5 69 6610.0 70 4947.5 71 3481.5 72 2487.5 73 1883.5 74 1418.0 75 946.5 76 534.0 77 347.5 78 255.5 79 161.5 80 90.5 81 41.0 82 16.5 83 6.0 84 6.0 85 3.0 86 1.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09029625815187056 2 0.0011703403349694092 3 0.0 4 2.100610857637401E-4 5 0.03580041075944885 6 1.5004363268838577E-4 7 0.004891422425641377 8 3.90113444989803E-4 9 1.8005235922606295E-4 10-11 4.5013089806515737E-4 12-13 0.0 14-15 6.001745307535431E-5 16-17 0.0 18-19 9.002617961303147E-5 20-21 0.0 22-23 1.8005235922606295E-4 24-25 9.002617961303147E-5 26-27 0.0013653970574643106 28-29 0.008492469610162635 30-31 0.036370576563664714 32-33 0.025477408830487908 34-35 0.02849328584752446 36-37 0.02585251791220887 38-39 0.025057286658960422 40-41 0.03105903196649586 42-43 0.022626579809408576 44-45 0.0217413223765471 46-47 0.014734284729999483 48-49 0.010458041198380489 50-51 0.0076372209038388355 52-53 0.0054015707767818884 54-55 0.004081186809124093 56-57 0.00243070684955185 58-59 9.002617961303147E-4 60-61 5.70165804215866E-4 62-63 0.0013804014207331491 64-65 0.002940855200692361 66-67 4.5013089806515737E-4 68-69 9.902879757433462E-4 70-71 2.85082902107933E-4 72-73 4.801396246028345E-4 74-75 4.3512653479631876E-4 76-77 3.90113444989803E-4 78-79 4.501308980651574E-5 80-81 5.251527144093502E-4 82-83 5.401570776781888E-4 84-85 2.700785388390944E-4 86-87 7.502181634419289E-5 88-89 0.0 90-91 3.0008726537677156E-5 92-93 1.5004363268838578E-5 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3332364.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.70069452275255 #Duplication Level Percentage of deduplicated Percentage of total 1 73.14636946998492 42.93742689705978 2 15.503191169978722 18.200961779935113 3 5.248920091084506 9.243457645232702 4 2.2791769468167704 5.351570788735644 5 1.2002978070374983 3.5229157453618987 6 0.677295008323665 2.3854612431235562 7 0.4452947620305305 1.8297378258975177 8 0.2891668537841482 1.357943612007099 9 0.1930456599606119 1.0198722882861937 >10 0.9420498484052955 9.57655923706472 >50 0.0549396877886203 2.1818938207573404 >100 0.01974165439758667 1.8462577501924509 >500 3.0662424605485804E-4 0.11741384293889529 >1k 1.53312120769854E-4 0.25942639288933916 >5k 5.110404025661801E-5 0.16910113051771564 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5637 0.16915919149288614 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4794 0.1438618350216243 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 3.0008726537677156E-5 0.0 7 0.0 0.0 0.0 3.0008726537677156E-5 0.0 8 0.0 0.0 0.0 3.0008726537677156E-5 0.0 9 6.001745307535431E-5 0.0 0.0 3.0008726537677156E-5 0.0 10-11 6.001745307535431E-5 0.0 0.0 3.0008726537677156E-5 0.0 12-13 6.001745307535431E-5 0.0 0.0 4.501308980651574E-5 0.0 14-15 6.001745307535431E-5 0.0 0.0 1.350392694195472E-4 0.0 16-17 6.001745307535431E-5 0.0 0.0 2.100610857637401E-4 0.0 18-19 6.001745307535431E-5 0.0 0.0 2.100610857637401E-4 0.0 20-21 6.001745307535431E-5 0.0 0.0 2.85082902107933E-4 0.0 22-23 6.001745307535431E-5 0.0 0.0 3.90113444989803E-4 0.0 24-25 6.001745307535431E-5 0.0 0.0 4.0511780825864163E-4 0.0 26-27 6.001745307535431E-5 0.0 0.0 5.551614409470274E-4 0.0 28-29 6.001745307535431E-5 0.0 0.0 0.0012603665145824406 0.0 30-31 6.001745307535431E-5 0.0 0.0 0.00411119553566177 0.0 32-33 6.001745307535431E-5 0.0 0.0 0.011718407712962928 0.0 34-35 6.001745307535431E-5 0.0 0.0 0.021291191478481943 0.0 36-37 6.001745307535431E-5 0.0 0.0 0.038651239780528174 0.0 38-39 6.001745307535431E-5 0.0 0.0 0.06933516266530307 0.0 40-41 6.001745307535431E-5 0.0 0.0 0.10389021127343831 0.0 42-43 6.001745307535431E-5 0.0 0.0 0.14270649904992372 0.0 44-45 6.001745307535431E-5 0.0 0.0 0.184973790378242 0.0 46-47 6.001745307535431E-5 0.0 0.0 0.23718897455380022 0.0 48-49 6.001745307535431E-5 0.0 0.0 0.29896193813160865 0.0 50-51 6.001745307535431E-5 0.0 0.0 0.3644709881633579 0.0 52-53 6.001745307535431E-5 0.0 0.0 0.43013008182779555 0.0 54-55 6.001745307535431E-5 0.0 0.0 0.5007406153709498 0.0 56-57 6.001745307535431E-5 0.0 0.0 0.5847800540397148 0.0 58-59 6.001745307535431E-5 0.0 0.0 0.682548485099467 0.0 60-61 6.001745307535431E-5 0.0 0.0 0.7863186614667546 0.0 62-63 9.002617961303147E-5 0.0 0.0 0.8950552820760278 0.0 64-65 9.002617961303147E-5 0.0 0.0 1.0047821906610443 0.0 66-67 9.002617961303147E-5 0.0 0.0 1.1329044486136568 0.0 68-69 9.002617961303147E-5 0.0 0.0 1.2764661963699044 0.0 70-71 9.002617961303147E-5 0.0 0.0 1.4337269277906013 0.0 72-73 9.002617961303147E-5 0.0 0.0 1.5964642518044247 0.0 74-75 9.002617961303147E-5 0.0 0.0 1.76589352183615 0.0 76-77 9.002617961303147E-5 0.0 0.0 1.9462609726908586 0.0 78-79 9.002617961303147E-5 0.0 0.0 2.149690129889772 0.0 80-81 9.002617961303147E-5 0.0 0.0 2.3806973067768107 0.0 82-83 9.002617961303147E-5 0.0 0.0 2.620121931457668 0.0 84-85 9.002617961303147E-5 0.0 0.0 2.8587213161587393 0.0 86-87 9.002617961303147E-5 0.0 0.0 3.1068034584457163 0.0 88 9.002617961303147E-5 0.0 0.0 3.311012842534609 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5425 0.0 41.722984 1 GTATCAA 8790 0.0 37.421253 1 TCAACGC 10175 0.0 31.323862 4 ATCAACG 10240 0.0 31.216845 3 CAACGCA 10460 0.0 30.604757 5 AACGCAG 10860 0.0 29.513708 6 TATCAAC 11035 0.0 29.266071 2 ACGCAGA 12325 0.0 25.891193 7 CGCAGAG 12445 0.0 25.677761 8 GTACATG 9170 0.0 25.145296 1 TATACCG 210 1.4297257E-9 24.623335 5 TACATGG 8985 0.0 24.223837 2 TATAACG 265 3.6379788E-11 23.060965 2 ACATGGG 9020 0.0 22.722702 3 GCAGAGT 14105 0.0 22.455875 9 AGAGTAC 11780 0.0 20.505058 10-11 GAGTACT 8680 0.0 20.465164 12-13 CATGGGG 6160 0.0 19.688791 4 CAGAGTA 14100 0.0 18.46434 10-11 GAGTACA 6480 0.0 18.227568 1 >>END_MODULE