##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033167_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 195486 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.031567477977962 33.0 31.0 34.0 15.0 34.0 2 29.106943719754867 33.0 30.0 34.0 12.0 34.0 3 29.4343022006691 34.0 31.0 34.0 16.0 34.0 4 32.256790767625304 37.0 35.0 37.0 16.0 37.0 5 32.90256591264848 37.0 35.0 37.0 20.0 37.0 6 32.71307408203145 37.0 35.0 37.0 17.0 37.0 7 33.04437657939699 37.0 35.0 37.0 22.0 37.0 8 32.6398309853391 37.0 35.0 37.0 15.0 37.0 9 33.74092773907083 39.0 35.0 39.0 13.0 39.0 10-11 34.00137349989257 39.0 35.0 39.0 13.0 39.0 12-13 34.098792240876584 39.0 35.0 39.0 14.5 39.0 14-15 35.41420869013638 40.0 36.5 41.0 17.0 41.0 16-17 35.6437596554229 40.0 36.5 41.0 22.5 41.0 18-19 35.64311255025935 40.0 35.5 41.0 24.5 41.0 20-21 35.6426240242268 40.0 35.0 41.0 25.5 41.0 22-23 35.59053077969778 40.0 35.0 41.0 25.5 41.0 24-25 35.495951116704006 40.0 35.0 41.0 26.0 41.0 26-27 35.07208444594498 40.0 35.0 41.0 25.0 41.0 28-29 34.80009821675209 40.0 35.0 41.0 24.0 41.0 30-31 34.5990889373152 40.0 35.0 41.0 18.5 41.0 32-33 34.430565360179244 40.0 35.0 41.0 5.5 41.0 34-35 34.149292020911986 40.0 35.0 41.0 2.0 41.0 36-37 33.84195543414874 40.0 34.5 41.0 2.0 41.0 38-39 33.6011734855693 40.0 34.0 41.0 2.0 41.0 40-41 33.526610601270676 40.0 34.0 41.0 2.0 41.0 42-43 33.31922490613138 40.0 33.5 41.0 2.0 41.0 44-45 33.15133308779146 40.0 33.0 41.0 2.0 41.0 46-47 32.943596983927236 39.5 33.0 41.0 2.0 41.0 48-49 32.71349354941019 39.0 33.0 41.0 2.0 41.0 50-51 32.52745720921192 39.0 33.0 41.0 2.0 41.0 52-53 32.47118975271887 39.0 32.5 41.0 2.0 41.0 54-55 32.295207840970704 39.0 32.0 41.0 2.0 41.0 56-57 32.02279447121533 38.0 31.5 41.0 2.0 41.0 58-59 31.805799392283845 38.0 31.0 40.0 2.0 41.0 60-61 31.380661530749006 37.0 31.0 40.0 2.0 41.0 62-63 31.207426618786002 37.0 30.5 40.0 2.0 41.0 64-65 30.894212373264583 36.5 30.0 39.5 2.0 41.0 66-67 30.406699200965797 36.0 30.0 39.0 2.0 41.0 68-69 29.939647852020094 35.0 29.5 38.5 2.0 40.0 70-71 29.330964877280216 35.0 28.5 37.0 2.0 39.0 72-73 28.894532089254476 35.0 28.5 37.0 2.0 39.0 74-75 28.55992244968949 35.0 28.0 36.0 2.0 38.5 76-77 28.198914500271123 34.5 28.0 35.5 2.0 37.0 78-79 27.749805612678145 34.0 26.5 35.0 2.0 37.0 80-81 27.26907041936507 34.0 26.0 35.0 2.0 36.0 82-83 27.000498756944232 34.0 25.5 35.0 2.0 36.0 84-85 26.759023664098706 34.0 25.0 35.0 2.0 36.0 86-87 26.659244140245338 34.0 25.0 35.0 2.0 35.0 88-89 26.21103301515198 34.0 24.5 35.0 2.0 35.0 90-91 25.861261164482364 34.0 21.5 35.0 2.0 35.0 92-93 25.524868788557747 34.0 19.5 35.0 2.0 35.0 94-95 25.20989226850005 34.0 13.5 35.0 2.0 35.0 96-97 24.93720010640148 34.0 2.0 35.0 2.0 35.0 98-99 24.675582906192773 34.0 2.0 35.0 2.0 35.0 100 24.426460206869034 33.0 2.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6570080836284156 1101 2 0.1901363271416372 1101 3 0.3842380624747612 1101 4 0.5773444675906205 1101 5 0.21948064289161806 1101 6 -0.06422927313369797 1101 7 0.08756030190200192 1101 8 0.07331568826715795 1101 9 0.8288445043745938 1101 10-11 0.5627509204196173 1101 12-13 0.4888742532569452 1101 14-15 -0.04617627743258623 1101 16-17 -0.12312879433927293 1101 18-19 -0.04153817747047839 1101 20-21 -0.23222817447973654 1101 22-23 0.0686950042624801 1101 24-25 0.12963846147899716 1101 26-27 0.16508029237384392 1101 28-29 0.378221978952503 1101 30-31 0.46648902714929363 1101 32-33 0.5288809209891596 1101 34-35 0.3534094369170049 1101 36-37 0.24408111105852015 1101 38-39 0.33479027641685377 1101 40-41 0.6084826874259619 1101 42-43 0.4734251889200607 1101 44-45 0.6290183375948288 1101 46-47 0.7489827686522048 1101 48-49 0.7873448186458916 1101 50-51 0.776779783673966 1101 52-53 0.7844832360223677 1101 54-55 0.5890021334294531 1101 56-57 0.6072670753213245 1101 58-59 0.7642789533575858 1101 60-61 0.41413099134635445 1101 62-63 0.6288942567227132 1101 64-65 0.5954251066307741 1101 66-67 0.6278177314123177 1101 68-69 0.9166312521855495 1101 70-71 1.0025785837151098 1101 72-73 0.9263053517060733 1101 74-75 0.7691495427561748 1101 76-77 0.9695165228596423 1101 78-79 1.0675101918734953 1101 80-81 1.0438905372209533 1101 82-83 1.1118684666990752 1101 84-85 1.0082118710705998 1101 86-87 1.1829238755676599 1101 88-89 1.327047005914629 1101 90-91 1.4321862757561554 1101 92-93 1.5723185911337545 1101 94-95 1.4653953126695356 1101 96-97 1.2813929145476024 1101 98-99 1.6449619814405274 1101 100 1.823740926743234 1102 1 0.11957641495427396 1102 2 -0.002720153910896528 1102 3 0.06352093100065659 1102 4 0.04786548429898119 1102 5 0.4499849821238371 1102 6 0.34073999707230485 1102 7 0.09315034483105933 1102 8 0.4701006414775719 1102 9 0.21982322515039954 1102 10-11 0.1646533880684018 1102 12-13 0.3090123056983387 1102 14-15 0.23118713712623418 1102 16-17 -0.06315936971220282 1102 18-19 0.12722089476771004 1102 20-21 0.14229698071343932 1102 22-23 0.25370689468529406 1102 24-25 0.3317216017859863 1102 26-27 0.29985900697280954 1102 28-29 0.2775394377992981 1102 30-31 0.3463339883896026 1102 32-33 0.3179726162237202 1102 34-35 0.015451192375792289 1102 36-37 0.04898552441285631 1102 38-39 0.02628013095220183 1102 40-41 0.07142935038652354 1102 42-43 0.02463893857260757 1102 44-45 -0.0689572021068301 1102 46-47 0.17736227388707704 1102 48-49 0.2579507669457186 1102 50-51 0.2529526117415415 1102 52-53 0.3834517032387694 1102 54-55 -0.12389820320849054 1102 56-57 0.030210958702532764 1102 58-59 -0.09194730440207266 1102 60-61 0.09518970223921741 1102 62-63 0.1418270217864901 1102 64-65 0.14666974049840675 1102 66-67 -0.22705734193769445 1102 68-69 0.09590639965767878 1102 70-71 0.15461385348872625 1102 72-73 0.14477256811176886 1102 74-75 0.3559419081782096 1102 76-77 0.34196471458885114 1102 78-79 0.03452585017054233 1102 80-81 -0.18450595433221295 1102 82-83 -0.03597427087774818 1102 84-85 0.12138707722273523 1102 86-87 -0.027281813304753655 1102 88-89 0.13883859923128838 1102 90-91 0.23587076261492257 1102 92-93 0.40321207147595217 1102 94-95 0.09306293674686827 1102 96-97 0.18043241545868938 1102 98-99 0.32817486633505766 1102 100 0.18073156882606511 1103 1 -0.09479672720344468 1103 2 0.10079444654372693 1103 3 0.03720514152697163 1103 4 -0.08059520230123951 1103 5 -0.1870436359329375 1103 6 -0.1570160208795528 1103 7 -0.13497811585554587 1103 8 0.1375250163682864 1103 9 -0.042751817106399415 1103 10-11 -0.18141527195374607 1103 12-13 0.03260908835265042 1103 14-15 -0.07147358444513685 1103 16-17 0.05897736747961346 1103 18-19 0.04873980833068714 1103 20-21 0.3085410793320804 1103 22-23 0.01740392796616419 1103 24-25 -0.23230474314610916 1103 26-27 0.006698780826049244 1103 28-29 -0.24994205663446678 1103 30-31 -0.23275909315145782 1103 32-33 -0.27279208623008344 1103 34-35 -0.04637488444416249 1103 36-37 -0.3262840448591646 1103 38-39 -0.6337565887540393 1103 40-41 -0.3643403063133661 1103 42-43 -0.3231928502016501 1103 44-45 -0.4121553136647904 1103 46-47 -0.30427787299174014 1103 48-49 -0.20205098161172685 1103 50-51 -0.467963049504597 1103 52-53 -0.4888744610090612 1103 54-55 -0.10376065002319734 1103 56-57 -0.093703478553401 1103 58-59 0.04081191923842198 1103 60-61 -0.021584792136252418 1103 62-63 -0.25158383092668757 1103 64-65 -0.22825011728558664 1103 66-67 -0.19810414499183793 1103 68-69 -0.10361857557109388 1103 70-71 -0.006617713801592373 1103 72-73 0.07800681652349795 1103 74-75 -0.09020252266634543 1103 76-77 -0.18844561355709644 1103 78-79 -0.14255930457679256 1103 80-81 -0.1297906777687139 1103 82-83 -0.16989919947832277 1103 84-85 -0.2133305307506852 1103 86-87 -0.1718000857300055 1103 88-89 -0.03458915620381475 1103 90-91 -0.06472083265231632 1103 92-93 0.37524972374616183 1103 94-95 0.16709977302360812 1103 96-97 0.34599166241328305 1103 98-99 0.32623688183893407 1103 100 0.3836516597510524 1104 1 1.3529833068232264 1104 2 1.1873154936871337 1104 3 1.4065866244313128 1104 4 1.2437009395131469 1104 5 1.070790731006312 1104 6 1.042177217691325 1104 7 1.048025012403059 1104 8 1.1601362890756022 1104 9 1.064020588359817 1104 10-11 1.1003886905071525 1104 12-13 1.2267257669103557 1104 14-15 1.1818604806445947 1104 16-17 1.2652229083390623 1104 18-19 1.0971043327804963 1104 20-21 1.2880701940897765 1104 22-23 1.311323032296336 1104 24-25 1.2822251948375367 1104 26-27 1.3474457211696063 1104 28-29 1.5763709887527213 1104 30-31 1.7110145745945502 1104 32-33 1.7804294713134041 1104 34-35 1.5413947220538304 1104 36-37 1.6761993534175446 1104 38-39 1.7447489817415445 1104 40-41 1.7873802359181212 1104 42-43 1.9478268523393751 1104 44-45 1.998263982751432 1104 46-47 2.0049806748376824 1104 48-49 2.09656051062251 1104 50-51 2.272926583336627 1104 52-53 2.3028768149911585 1104 54-55 2.2181206956768236 1104 56-57 2.0231946060742274 1104 58-59 2.359249436388968 1104 60-61 1.9834231657145764 1104 62-63 2.3852774829419268 1104 64-65 2.4862414034871456 1104 66-67 2.1868225878987815 1104 68-69 2.184784914906924 1104 70-71 1.9875511023994932 1104 72-73 1.976818627071765 1104 74-75 2.1210921373413356 1104 76-77 2.308467289137596 1104 78-79 2.3093094183253555 1104 80-81 2.0695397651268443 1104 82-83 1.9503466706715074 1104 84-85 1.9587236923984293 1104 86-87 2.360003876730893 1104 88-89 2.3787525923281017 1104 90-91 2.1905615338478768 1104 92-93 1.9529348123801533 1104 94-95 2.3408278889338234 1104 96-97 2.1369197124654207 1104 98-99 2.1510544000871086 1104 100 2.118518022738815 1105 1 0.4614203278898046 1105 2 0.39821504430146604 1105 3 0.29661701640990756 1105 4 0.054127726015991584 1105 5 0.19716421465438572 1105 6 0.17291467775314828 1105 7 0.10752315511548005 1105 8 0.443989576344741 1105 9 -0.18072575551113346 1105 10-11 0.05066403663801822 1105 12-13 -0.04220427958677675 1105 14-15 -0.09051139248837359 1105 16-17 -0.20362531360030545 1105 18-19 -0.3635029872704152 1105 20-21 0.10652358006790763 1105 22-23 -0.026631873280720697 1105 24-25 0.4782015046798591 1105 26-27 0.45419429898086605 1105 28-29 0.8330857976988781 1105 30-31 0.6764534399606816 1105 32-33 0.612546259634577 1105 34-35 0.35452951177770586 1105 36-37 0.4258097126663216 1105 38-39 0.4317051081929577 1105 40-41 0.4410740302397613 1105 42-43 0.15029068068963625 1105 44-45 0.17028388040579756 1105 46-47 0.1606031908778931 1105 48-49 -0.3135753441194211 1105 50-51 -0.09405006419434869 1105 52-53 -0.003510695723953461 1105 54-55 -0.04462705090083574 1105 56-57 -0.17001525443438226 1105 58-59 -0.14100383009933282 1105 60-61 0.005587230446892022 1105 62-63 -0.09677956046405356 1105 64-65 -0.2006432888265408 1105 66-67 -0.3112941484702887 1105 68-69 -0.34234706940354087 1105 70-71 -0.07710978026668514 1105 72-73 -0.0996548643691284 1105 74-75 -0.00905436758112188 1105 76-77 0.18113269163649193 1105 78-79 0.1270939210138735 1105 80-81 0.06916311240066264 1105 82-83 0.24567487806429256 1105 84-85 0.7631565053448881 1105 86-87 0.8565425680574883 1105 88-89 0.7936290762099603 1105 90-91 0.6462269103707214 1105 92-93 0.2543803795672055 1105 94-95 0.6604372091405892 1105 96-97 0.5709535332826263 1105 98-99 0.7586212234759451 1105 100 0.42115199421711225 1106 1 -0.1280488716490673 1106 2 -0.41297236485095823 1106 3 -0.28154949153455533 1106 4 -0.7269734719189493 1106 5 -0.437202483756721 1106 6 -0.5617602758832589 1106 7 -0.467473085674456 1106 8 -0.44015156946248624 1106 9 -0.43685326791652557 1106 10-11 -0.2857915294990221 1106 12-13 -0.8206552344261269 1106 14-15 -0.46344382695281894 1106 16-17 -0.49507060456316765 1106 18-19 -0.26396537010837307 1106 20-21 -0.026389557118200457 1106 22-23 -0.12361322759880267 1106 24-25 -0.08412619819218037 1106 26-27 0.1922462763253563 1106 28-29 -0.3140371535316362 1106 30-31 -0.19724336721689895 1106 32-33 -0.3047072614921831 1106 34-35 -0.33163910132439867 1106 36-37 -0.4146431468394667 1106 38-39 -0.429623897871906 1106 40-41 -0.5517145517862048 1106 42-43 -0.5258032494391855 1106 44-45 -0.41427328464859414 1106 46-47 -0.5149895112422982 1106 48-49 -0.5467904711807208 1106 50-51 -0.6814015753967908 1106 52-53 -0.5809003002550597 1106 54-55 -0.4684090668398895 1106 56-57 -0.4895919673811022 1106 58-59 -0.733160864834435 1106 60-61 -0.546365052646685 1106 62-63 -0.41531622529288725 1106 64-65 -0.6521620096926632 1106 66-67 -0.5170043885585045 1106 68-69 -0.37896804078341617 1106 70-71 -0.26883575891437417 1106 72-73 -0.4079351510715483 1106 74-75 -0.19667027655811609 1106 76-77 -0.6137209497849909 1106 78-79 -0.30866117898111156 1106 80-81 -0.17772300859578039 1106 82-83 -0.1518302595227965 1106 84-85 0.024597034641665516 1106 86-87 0.19037295249937358 1106 88-89 0.26673192819732705 1106 90-91 0.39524951575391043 1106 92-93 0.20849922072805427 1106 94-95 0.13313281337095617 1106 96-97 0.42999568655815423 1106 98-99 0.6607439240924542 1106 100 0.5704302258846958 1107 1 0.23339129251472457 1107 2 0.39609395358948873 1107 3 0.5469646396099286 1107 4 0.789792687954197 1107 5 0.08418078272961793 1107 6 0.6247894862657901 1107 7 0.7065545236903432 1107 8 0.8656034906998116 1107 9 0.48092492441627854 1107 10-11 0.7067115019346204 1107 12-13 0.6844165872322989 1107 14-15 0.6565426481894363 1107 16-17 1.0334831684057733 1107 18-19 0.9010029164320628 1107 20-21 1.0163536571342036 1107 22-23 0.8597955029300692 1107 24-25 0.5894350367672558 1107 26-27 0.5162715538181288 1107 28-29 0.37785259555892736 1107 30-31 0.6701314112552552 1107 32-33 0.3327449351312666 1107 34-35 0.3111414228804392 1107 36-37 0.4396498295619864 1107 38-39 0.523353902706404 1107 40-41 0.4653664108105673 1107 42-43 0.35037434593438377 1107 44-45 0.23286784024647744 1107 46-47 0.39047639678866375 1107 48-49 0.5518826936359957 1107 50-51 0.2878876700094253 1107 52-53 0.3363881989521005 1107 54-55 0.4935231223215695 1107 56-57 0.41502280736006014 1107 58-59 0.5040021796576788 1107 60-61 0.6354510092878343 1107 62-63 0.743449281656094 1107 64-65 0.8039288400415039 1107 66-67 0.6888649333331109 1107 68-69 0.5987042845593642 1107 70-71 0.7535152344631086 1107 72-73 0.6938510629010288 1107 74-75 0.6400170660405209 1107 76-77 0.905738522279183 1107 78-79 0.617884852172697 1107 80-81 0.4406544748479284 1107 82-83 0.6024953630870336 1107 84-85 0.6596590624375835 1107 86-87 0.5474525159729282 1107 88-89 1.2506769892923764 1107 90-91 0.7010512133567453 1107 92-93 0.40599712204322813 1107 94-95 0.49063211380934746 1107 96-97 0.45266148265926276 1107 98-99 0.4172964844879381 1107 100 0.5064669761878413 1108 1 -0.7615773150366643 1108 2 -0.9439765719115023 1108 3 -0.5533621858824596 1108 4 -0.671504293210333 1108 5 -0.921151633198825 1108 6 -0.6836735738529001 1108 7 -0.5667962976737257 1108 8 -1.0068700622373186 1108 9 -0.6211865402779679 1108 10-11 -0.9411555316940081 1108 12-13 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48-49 -0.9251644756532365 2215 50-51 -0.8628622054725099 2215 52-53 -1.0576745950488942 2215 54-55 -0.9532799915525061 2215 56-57 -0.7361836343747044 2215 58-59 -0.439019274344421 2215 60-61 -1.0170190602578124 2215 62-63 -0.6513052793946521 2215 64-65 -0.741072912435019 2215 66-67 -1.0744262175827899 2215 68-69 -1.0301165754454296 2215 70-71 -1.0233622213099167 2215 72-73 -0.9014421540924573 2215 74-75 -0.7426361834199007 2215 76-77 -0.9238792533669269 2215 78-79 -0.6754566593844231 2215 80-81 -0.7790461283729364 2215 82-83 -0.9122023808300277 2215 84-85 -0.8121168419325393 2215 86-87 -1.080773424369081 2215 88-89 -1.1453919291529004 2215 90-91 -1.050756604533273 2215 92-93 -1.2840220629629506 2215 94-95 -1.3468487473022748 2215 96-97 -1.530251871211867 2215 98-99 -1.666799329860229 2215 100 -1.678373179505865 2216 1 -0.326512379971728 2216 2 -0.40845327168231904 2216 3 -0.4150886946677943 2216 4 -0.46676785317977476 2216 5 -0.8802807393867553 2216 6 -0.3807705944793298 2216 7 -0.6498749836935858 2216 8 -0.617450658112034 2216 9 -0.8784439887118864 2216 10-11 -0.7452044239781941 2216 12-13 -0.7547235480511958 2216 14-15 -0.44959468934642643 2216 16-17 -0.38513424845862687 2216 18-19 -0.5471148014558125 2216 20-21 -0.7067578632114433 2216 22-23 -0.706642149795087 2216 24-25 -0.5304431372597378 2216 26-27 -0.9687746621396585 2216 28-29 -0.9056052788611524 2216 30-31 -0.907842523652846 2216 32-33 -1.0278280719162254 2216 34-35 -0.916936984882426 2216 36-37 -0.9908934292827922 2216 38-39 -0.8654621896546857 2216 40-41 -0.9386336600184819 2216 42-43 -0.8706477173159612 2216 44-45 -0.8576339836016658 2216 46-47 -0.7429212729354546 2216 48-49 -0.5775485490597561 2216 50-51 -1.032666921484008 2216 52-53 -1.0004039531887052 2216 54-55 -0.9001801747708953 2216 56-57 -1.0899440800571583 2216 58-59 -1.1004972360026208 2216 60-61 -1.191974000451161 2216 62-63 -1.1098726009801645 2216 64-65 -1.0353246217285133 2216 66-67 -1.1844838643436972 2216 68-69 -1.3581519678753828 2216 70-71 -1.5141566145293446 2216 72-73 -1.4969801160485758 2216 74-75 -1.5017561393283216 2216 76-77 -1.5672401935900666 2216 78-79 -1.3699325505258884 2216 80-81 -1.400316591483481 2216 82-83 -1.5354353660980777 2216 84-85 -1.4421311419042375 2216 86-87 -1.3345197190378713 2216 88-89 -1.0146663545472663 2216 90-91 -1.1454976409833044 2216 92-93 -1.1010680761010505 2216 94-95 -1.1561079789767135 2216 96-97 -1.1817012329266703 2216 98-99 -1.0935492144177523 2216 100 -1.2573358357256055 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6671.0 3 904.0 4 733.0 5 865.0 6 2072.0 7 4600.0 8 4392.0 9 2348.0 10 1385.0 11 1356.0 12 1281.0 13 940.0 14 755.0 15 737.0 16 685.0 17 673.0 18 654.0 19 582.0 20 609.0 21 648.0 22 762.0 23 1482.0 24 3254.0 25 2119.0 26 2256.0 27 1748.0 28 1953.0 29 2301.0 30 2754.0 31 3351.0 32 4332.0 33 5628.0 34 7903.0 35 15060.0 36 21791.0 37 38653.0 38 39821.0 39 7415.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.48961524841804 21.457714276916445 12.203558580057788 16.84911189460773 2 14.94348242500602 31.804516284334362 29.681586077265443 23.570415213394178 3 25.018810173360702 33.26918253801702 25.334104507787668 16.377902780834606 4 11.733038574513596 23.837084344008105 34.16643638633875 30.263440695139547 5 10.511905919439938 47.972692892271 27.962662545354057 13.552738642935003 6 24.361020729735813 41.93883397794012 13.481490576462644 20.21865471586142 7 22.346705955779285 43.31675823894738 17.352179829542546 16.98435597573079 8 21.518799531354723 35.35969548289394 17.0318791345411 26.08962585121024 9 20.006344236210243 30.785406210188636 19.291082766700946 29.917166786900175 10-11 22.261789641181498 33.43336045065926 20.791058445512085 23.513791462647163 12-13 27.52020873836079 33.494576895528496 19.979279648009822 19.00593471810089 14-15 22.841494268457716 35.21630464535043 24.165338558787642 17.776862527404216 16-17 18.77084910873258 37.65016474633158 23.914311442195526 19.664674702740314 18-19 18.167934244556832 40.707797074977684 22.59742186680583 18.526846813659656 20-21 23.18185423110477 34.015897502807775 20.05863707183041 22.743611194257046 22-23 20.447359535040775 34.791106017661086 21.48134126001494 23.280193187283203 24-25 24.368341878242717 34.87116118666789 20.078490365230916 20.682006569858473 26-27 22.31562467223159 31.645864298121516 26.23912448420444 19.799386545442452 28-29 19.205482676053457 38.94025131490085 22.359453983586764 19.494812025458934 30-31 20.77528383072995 35.23491821479553 21.321968165934173 22.66782978854035 32-33 20.17483716146726 35.58476046234375 23.660067847256688 20.5803345289323 34-35 22.588591615228573 34.082671011938416 22.570685275616935 20.758052097216076 36-37 20.936312151799005 33.661092040170054 25.658544321605987 19.744051486424958 38-39 20.960220601589537 35.49731282502539 23.97379588725376 19.568670686131313 40-41 19.568681719236405 36.92168383464512 23.00040159512561 20.509232850992863 42-43 18.496274450120094 37.08258621968594 20.786598763521585 23.634540566672378 44-45 20.227607587942785 33.92319970532261 22.184193832238524 23.66499887449608 46-47 22.14559955390601 36.285295524480176 22.43336087315737 19.13574404845645 48-49 20.606102461396013 34.69818931493745 23.030409993937955 21.665298229728588 50-51 16.651020977160705 35.130925005563384 26.91322909126254 21.304824926013367 52-53 19.672076634820755 36.154702068065845 21.102712657790736 23.070508639322668 54-55 17.612919892675265 32.79312876154521 23.463089857608008 26.130861488171515 56-57 21.271267942828494 31.700616936597743 22.4936311271626 24.534483993411158 58-59 24.439908635855566 30.975309684036596 21.93583536037937 22.648946319728466 60-61 20.606764392174114 32.31131653191255 25.792985965280245 21.288933110633085 62-63 19.39160483217082 32.75706396769004 24.43300261150032 23.418328588638822 64-65 18.865723866452836 35.72435243232736 22.76021004545187 22.649713655767936 66-67 20.64844845049672 31.83873707042081 21.35055913076396 26.162255348318514 68-69 19.99416818643387 32.78962755685379 23.00317936151873 24.213024895193612 70-71 23.971689981251135 32.46111464943715 21.351964538299608 22.215230831012107 72-73 21.889475770868767 29.758923661290943 26.65114910923252 21.700451458607766 74-75 20.33543245054456 35.08407552729449 23.173333196916293 21.407158825244654 76-77 21.86388913046815 30.17782507047009 23.4753648841528 24.482920914908966 78-79 22.787941107257232 30.747567451425766 25.275995784605627 21.18849565671138 80-81 24.340390483717908 30.033431299574627 24.17796603666444 21.448212180043022 82-83 22.640597303539774 28.756103612417732 27.166158938195252 21.43714014584724 84-85 22.343949435601246 30.876052252607135 25.32414879766311 21.455849514128513 86-87 22.609458571110665 31.42855681204869 24.348824776760022 21.613159840080627 88-89 22.1098830987075 30.772001317197663 22.53745780851239 24.58065777558245 90-91 23.373281943879835 28.153221532112404 24.215907745982413 24.257588778025347 92-93 25.171221422359896 30.978341969445143 23.89458626420842 19.95585034398654 94-95 23.154851024639715 30.609793116931893 24.074788666450576 22.160567191977815 96-97 19.363128434136602 31.29283024303912 26.863179881875993 22.480861440948285 98-99 21.77981972341126 33.8414759631215 21.990502551860388 22.388201761606847 100 20.57127275907662 30.046868649452342 23.43020892819476 25.951649663276278 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5509.0 1 3476.0 2 1448.5 3 1329.5 4 1003.5 5 655.0 6 452.0 7 419.0 8 467.0 9 448.0 10 342.5 11 258.5 12 215.0 13 199.0 14 184.5 15 171.5 16 173.0 17 187.0 18 214.0 19 226.0 20 250.5 21 293.5 22 369.5 23 487.0 24 619.5 25 781.5 26 998.5 27 1190.0 28 1360.0 29 1616.0 30 1776.5 31 1904.0 32 2097.5 33 2348.0 34 2499.0 35 2492.0 36 2610.5 37 2748.0 38 2815.5 39 3133.5 40 3609.0 41 4051.0 42 4357.0 43 4234.0 44 4003.5 45 4025.0 46 5221.0 47 11353.5 48 24235.0 49 24094.5 50 12573.5 51 7399.0 52 5274.5 53 4728.5 54 4334.5 55 3352.5 56 2491.5 57 2092.0 58 1882.5 59 1836.5 60 1743.0 61 1745.0 62 1771.0 63 1778.5 64 1514.5 65 982.5 66 655.0 67 461.5 68 336.0 69 289.5 70 229.5 71 231.5 72 247.0 73 217.0 74 212.5 75 216.0 76 180.5 77 111.0 78 77.0 79 93.5 80 82.5 81 58.0 82 51.0 83 22.0 84 5.0 85 4.0 86 2.5 87 1.0 88 0.5 89 0.5 90 0.5 91 1.5 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3238083545624751 2 0.12123630336699302 3 0.05780465097244815 4 0.03683128203554219 5 0.04041210112233101 6 0.009207820508885547 7 0.006138547005923698 8 0.014834821930982268 9 0.01688100426629017 10-11 0.01995027776925202 12-13 0.013300185179501345 14-15 0.01662523147437668 16-17 0.017392549850117144 18-19 0.01764832264203063 20-21 0.02378686964795433 22-23 0.014323276347155295 24-25 0.02455418802369479 26-27 0.018159868225857606 28-29 0.017392549850117144 30-31 0.01790409543394412 32-33 0.024042642439867815 34-35 0.012532866803760884 36-37 0.008696274925058572 38-39 0.010486684468452984 40-41 0.00792895654931811 42-43 0.00588277421401021 44-45 0.009207820508885547 46-47 0.00588277421401021 48-49 0.003836591878702311 50-51 0.00588277421401021 52-53 0.00690586538166416 54-55 0.0023019551272213867 56-57 0.002046182335307899 58-59 0.0023019551272213867 60-61 0.0033250462948753363 62-63 0.0023019551272213867 64-65 0.0023019551272213867 66-67 0.002046182335307899 68-69 0.0033250462948753363 70-71 0.003836591878702311 72-73 0.004348137462529286 74-75 0.003580819086788824 76-77 0.006650092589750673 78-79 0.006138547005923698 80-81 0.005371228630183236 82-83 0.003836591878702311 84-85 0.006394319797837185 86-87 0.006394319797837185 88-89 0.5800926920597894 90-91 0.5898120581525019 92-93 0.5867427846495401 94-95 0.5754887818053467 96-97 0.56986178038325 98-99 0.5719079627185579 100 0.5693502347994229 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 195486.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.49870578967292 #Duplication Level Percentage of deduplicated Percentage of total 1 82.09236976727429 29.141728819455103 2 7.333381367533684 5.20651095219095 3 2.844585344765473 3.029372947423345 4 1.5779234815188412 2.2405696571621494 5 1.1340874702788386 2.012931872359146 6 0.8040925138698753 1.7126546146527115 7 0.5576770660710426 1.3857769865872747 8 0.4841847395345486 1.3750345293269084 9 0.36313855465091144 1.1601853841195788 >10 2.5232365444196265 16.446702065621068 >50 0.15995388716766337 3.7276326693471655 >100 0.08357950861013042 5.93699804589587 >500 0.017292312126233878 4.304144542320166 >1k 0.021615390157792347 14.700285442435776 >5k 0.00288205202103898 7.6194714711027896 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7536 3.855007519720082 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7359 3.764463951382708 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4626 2.3664098707835852 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3196 1.6348996859110114 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2264 1.1581392017842709 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2020 1.0333220793304891 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1938 0.9913753414566773 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1923 0.9837021576992726 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1878 0.9606826064270588 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1855 0.9489170579990384 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1635 0.8363770295571038 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1476 0.7550412817286148 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1383 0.7074675424327062 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1329 0.6798440809060495 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1169 0.5979967874937335 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1023 0.5233111322549953 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1022 0.5227995866711682 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 952 0.48699139580328005 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 944 0.48289903113266425 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 903 0.4619256621957583 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 786 0.40207482888800217 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 710 0.3631973645171521 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 707 0.3616627277656712 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 620 0.3171582619727244 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 620 0.3171582619727244 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 593 0.3033465312093961 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 543 0.27776925201804736 No Hit GAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 519 0.26549215800619996 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 517 0.264469066838546 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 477 0.24400724348546698 No Hit GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 452 0.2312186038897926 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 400 0.20461823353078992 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 338 0.1729024073335175 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 332 0.16983313383055565 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 323 0.16522922357611286 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 309 0.1580675854025352 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 306 0.1565329486510543 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 291 0.14885976489364966 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 287 0.1468135825583418 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 285 0.1457904913906878 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 277 0.141698126720072 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 273 0.13965194438476414 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 270 0.1381173076332832 No Hit AAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 259 0.1324903062111865 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 254 0.1299325782920516 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 229 0.11714393869637722 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 211 0.10793611818749169 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 211 0.10793611818749169 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 207 0.1058899358521838 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 202 0.10333220793304891 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 200 0.10230911676539496 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 199 0.10179757118156799 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 196 0.10026293443008708 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 196 0.10026293443008708 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.115455838269748E-4 0.0 3 0.0 0.0 0.0 5.115455838269748E-4 0.0 4 0.0 0.0 0.0 0.0010230911676539496 0.0 5 0.0 0.0 0.0 0.0010230911676539496 0.0 6 0.0 0.0 0.0 0.0010230911676539496 0.0 7 0.0 0.0 0.0 0.0010230911676539496 0.0 8 0.0 0.0 0.0 0.0010230911676539496 0.0 9 0.0 0.0 0.0 0.0010230911676539496 0.0 10-11 0.0 2.557727919134874E-4 0.0 0.0010230911676539496 0.0 12-13 0.0 5.115455838269748E-4 0.0 0.0015346367514809244 0.0 14-15 0.0 5.115455838269748E-4 0.0 0.0015346367514809244 0.0 16-17 0.0 5.115455838269748E-4 0.0 0.0015346367514809244 0.0 18-19 0.0 5.115455838269748E-4 0.0 0.0015346367514809244 0.0 20-21 0.0 5.115455838269748E-4 0.0 0.0028135007110483617 0.0 22-23 0.0 5.115455838269748E-4 0.0 0.008696274925058572 0.0 24-25 0.0 5.115455838269748E-4 0.0 0.020206050561165506 0.0 26-27 0.0 5.115455838269748E-4 0.0 0.04578332975251424 0.0 28-29 0.0 5.115455838269748E-4 0.0 0.10742457260366471 0.0 30-31 0.0 5.115455838269748E-4 0.0 0.24477456186120744 0.0 32-33 0.0 5.115455838269748E-4 0.0 0.4491370226000839 0.0 34-35 0.0 5.115455838269748E-4 0.0 0.7460892340116427 0.0 36-37 0.0 5.115455838269748E-4 0.0 1.1683701134608104 0.0 38-39 0.0 5.115455838269748E-4 0.0 1.7067718404387016 0.0 40-41 0.0 5.115455838269748E-4 0.0 2.3162784035685418 0.0 42-43 0.0 5.115455838269748E-4 0.0 2.805060208915217 0.0 44-45 0.0 5.115455838269748E-4 0.0 3.2621261880646184 0.0 46-47 0.0 5.115455838269748E-4 0.0 3.776485272602642 0.0 48-49 0.0 5.115455838269748E-4 0.0 4.302098359984859 0.0 50-51 0.0 5.115455838269748E-4 0.0 4.800343758632332 0.0 52-53 0.0 5.115455838269748E-4 0.0 5.254084691486858 0.0 54-55 0.0 5.115455838269748E-4 0.0 5.704756350838423 0.0 56-57 0.0 5.115455838269748E-4 0.0 6.268735357007663 0.0 58-59 0.0 5.115455838269748E-4 0.0 6.762376845400693 0.0 60-61 0.0 5.115455838269748E-4 0.0 7.269062746181312 0.0 62-63 0.0 5.115455838269748E-4 0.0 7.732778817920465 0.0 64-65 0.0 5.115455838269748E-4 0.0 8.178590794225673 0.0 66-67 0.0 5.115455838269748E-4 0.0 8.70113460810493 0.0 68-69 0.0 5.115455838269748E-4 0.0 9.325987538749578 0.0 70-71 0.0 5.115455838269748E-4 0.0 9.97079074716348 0.0 72-73 0.0 5.115455838269748E-4 0.0 10.612780454866332 0.0 74-75 0.0 5.115455838269748E-4 0.0 11.262699119118505 0.0 76-77 0.0 5.115455838269748E-4 0.0 11.8410014016349 0.0 78-79 0.0 5.115455838269748E-4 0.0 12.664845564388242 0.0 80-81 0.0 5.115455838269748E-4 0.0 13.476412633129739 0.0 82-83 0.0 5.115455838269748E-4 0.0 14.193343768863244 0.0 84-85 0.0 5.115455838269748E-4 0.0 14.970637283488331 0.0 86-87 0.0 5.115455838269748E-4 0.0 15.701380149985166 0.0 88 0.0 5.115455838269748E-4 0.0 16.30244621098186 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAGAGG 35 3.644027E-6 67.09076 3 TAAGTTC 25 0.0052456446 56.356243 5 CGAGTGT 25 0.0052509606 56.341827 6 TACATGA 60 2.5363988E-8 54.84693 2 TACAAGA 45 1.606617E-5 52.235176 2 GGAAACA 220 0.0 47.0961 1 CAAGAAA 125 0.0 45.084995 4 ACATGAA 85 7.925337E-9 44.200974 3 AGAGGTC 60 8.837528E-5 39.13628 5 ACAAGAA 145 0.0 38.86638 3 ATATCCA 25 0.0016499789 37.83221 90-91 ACAACAC 615 0.0 33.599926 5 TGGGATG 70 2.1916429E-4 33.5368 6 AACAACA 720 0.0 33.26584 4 GAAAACA 375 0.0 32.653297 1 TAACAAC 90 2.5471678E-5 31.397402 1 TACAAGG 60 0.0041475664 31.341105 2 AACAAGA 105 2.04981E-6 31.341103 2 ATGGGAT 135 1.3253157E-8 31.309025 5 CATGGGT 120 1.651606E-7 31.309023 4 >>END_MODULE