##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033167_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	195486
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51818544550505	33.0	31.0	34.0	31.0	34.0
2	32.51674288695866	34.0	31.0	34.0	31.0	34.0
3	32.60166968478561	34.0	31.0	34.0	31.0	34.0
4	36.09570506327819	37.0	35.0	37.0	35.0	37.0
5	36.08971997994741	37.0	35.0	37.0	35.0	37.0
6	36.270802001166324	37.0	37.0	37.0	35.0	37.0
7	36.26113890508783	37.0	37.0	37.0	35.0	37.0
8	36.271707436849695	37.0	37.0	37.0	35.0	37.0
9	38.0261450947894	39.0	39.0	39.0	35.0	39.0
10-11	38.00559630868707	39.0	38.5	39.0	35.0	39.0
12-13	37.9756325261144	39.0	38.5	39.0	35.0	39.0
14-15	39.16224435509449	41.0	39.0	41.0	36.0	41.0
16-17	39.239411006414784	40.5	39.0	41.0	36.0	41.0
18-19	39.424176155837245	41.0	39.0	41.0	36.0	41.0
20-21	39.42299448553861	41.0	39.0	41.0	36.0	41.0
22-23	39.23589413052597	41.0	39.0	41.0	35.5	41.0
24-25	39.04114105357928	41.0	39.0	41.0	35.0	41.0
26-27	38.75987282976786	40.5	38.0	41.0	35.0	41.0
28-29	38.61929243014845	40.0	38.0	41.0	35.0	41.0
30-31	38.26427723724461	40.0	37.5	41.0	35.0	41.0
32-33	37.893347861227916	40.0	35.5	41.0	34.5	41.0
34-35	37.56453659085561	40.0	35.0	41.0	34.0	41.0
36-37	37.2265149422465	40.0	35.0	41.0	33.0	41.0
38-39	36.964130423662056	40.0	35.0	41.0	33.0	41.0
40-41	36.789698495032894	40.0	35.0	41.0	33.0	41.0
42-43	36.43091321117625	40.0	35.0	41.0	31.5	41.0
44-45	35.907980622653284	40.0	35.0	41.0	30.0	41.0
46-47	35.08919308799607	40.0	35.0	41.0	22.0	41.0
48-49	34.32776260192546	40.0	35.0	41.0	3.5	41.0
50-51	33.935673142833764	39.0	35.0	41.0	2.0	41.0
52-53	33.80820877198367	40.0	35.0	41.0	2.0	41.0
54-55	33.59357447592155	39.0	35.0	41.0	2.0	41.0
56-57	33.366530083995784	39.0	35.0	41.0	2.0	41.0
58-59	33.145972090072945	39.0	35.0	41.0	2.0	41.0
60-61	32.70852644179122	38.0	34.0	40.5	2.0	41.0
62-63	32.561966074296876	37.5	34.0	40.0	2.0	41.0
64-65	32.21192566219576	37.0	34.0	39.5	2.0	41.0
66-67	31.685841952876423	36.5	33.0	39.0	2.0	41.0
68-69	31.121080281963927	36.0	33.0	39.0	2.0	40.5
70-71	30.407719734405532	35.0	32.0	37.5	2.0	39.5
72-73	29.91816293749936	35.0	31.5	37.0	2.0	39.0
74-75	29.617059533675047	35.0	31.5	37.0	2.0	39.0
76-77	29.25577790736933	35.0	31.0	36.0	2.0	37.0
78-79	28.91636741250013	35.0	31.0	35.5	2.0	37.0
80-81	28.60988766458979	35.0	30.5	35.0	2.0	36.5
82-83	28.284063820427036	35.0	29.5	35.0	2.0	36.0
84-85	28.132820253112754	35.0	29.5	35.0	2.0	36.0
86-87	28.097700091055117	35.0	30.0	35.0	2.0	35.5
88-89	27.895327542637325	35.0	29.0	35.0	2.0	35.0
90-91	27.697587039481085	35.0	29.0	35.0	2.0	35.0
92-93	27.51265052228804	35.0	29.0	35.0	2.0	35.0
94-95	27.32258576061713	34.0	29.0	35.0	2.0	35.0
96-97	27.22236119210583	34.0	29.0	35.0	2.0	35.0
98-99	27.156228579028678	35.0	29.0	35.0	2.0	35.0
100	27.01051737720348	34.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0056908308320871015
1101	2	0.18352463815519116
1101	3	0.13749797704728906
1101	4	0.0965705056677777
1101	5	0.0929583385339896
1101	6	0.021809519353830353
1101	7	0.013892157741949518
1101	8	-0.012993686719603659
1101	9	0.16848187505087253
1101	10-11	0.013784614894134961
1101	12-13	-0.20038710156786976
1101	14-15	0.04152200418261742
1101	16-17	0.1485413570541425
1101	18-19	0.26578819210092064
1101	20-21	0.2778550156929782
1101	22-23	0.21514637443970486
1101	24-25	0.06804376809652979
1101	26-27	-0.00538383464069625
1101	28-29	0.23984396074279601
1101	30-31	0.2757299492158438
1101	32-33	0.6166366789133946
1101	34-35	0.7801131970464397
1101	36-37	0.6227355969354917
1101	38-39	0.48403323851444924
1101	40-41	0.575935132888695
1101	42-43	0.7837147798488928
1101	44-45	0.8535871514363507
1101	46-47	1.066250608292222
1101	48-49	1.16074717525035
1101	50-51	1.2645393601354513
1101	52-53	1.4410191575200813
1101	54-55	1.3478633004557423
1101	56-57	1.4698883582825886
1101	58-59	1.392465979973693
1101	60-61	1.5767532256548904
1101	62-63	1.7942261659372605
1101	64-65	1.9500893317817258
1101	66-67	2.1994620850979416
1101	68-69	2.1968247531549068
1101	70-71	1.9280612599120666
1101	72-73	1.692385873273743
1101	74-75	1.5928444033788232
1101	76-77	1.6383023534295447
1101	78-79	1.5006978623022391
1101	80-81	1.41378682980854
1101	82-83	1.5223750224763606
1101	84-85	1.6926744410808858
1101	86-87	1.5493794001133132
1101	88-89	1.3871483268645974
1101	90-91	1.6702760513243788
1101	92-93	1.681204337112927
1101	94-95	1.6219604887699006
1101	96-97	1.679793626629042
1101	98-99	1.5656686261231094
1101	100	1.7043017685047204
1102	1	0.22032068351438028
1102	2	0.19905939833925856
1102	3	0.09812060282325064
1102	4	0.10322605401701423
1102	5	0.18886926845770802
1102	6	0.07249051629629122
1102	7	0.17708435021237534
1102	8	0.010771716782663532
1102	9	0.08921753705401869
1102	10-11	0.011198061881380283
1102	12-13	0.0613180159465756
1102	14-15	0.04908863687665388
1102	16-17	-0.1291695049242989
1102	18-19	-0.05895160838704783
1102	20-21	0.25851377600058356
1102	22-23	0.2581140386665126
1102	24-25	0.28697424772839497
1102	26-27	0.5618819857984789
1102	28-29	0.3684149481111447
1102	30-31	0.5611464228561616
1102	32-33	0.2603177685085001
1102	34-35	0.5445592886490189
1102	36-37	0.81391660932006
1102	38-39	0.6570620534871239
1102	40-41	0.7546234802743399
1102	42-43	0.7319065090560883
1102	44-45	0.8621189158515534
1102	46-47	0.720679404734355
1102	48-49	0.24924589355542537
1102	50-51	0.021781708571161573
1102	52-53	-0.21970229774413497
1102	54-55	-0.31045690708618423
1102	56-57	0.01060147480872331
1102	58-59	0.19020525543000844
1102	60-61	0.3138513448061886
1102	62-63	0.2167762755762226
1102	64-65	0.14704569238581655
1102	66-67	0.22310395173990116
1102	68-69	0.4243410445783624
1102	70-71	0.519486257595748
1102	72-73	0.6611001634000608
1102	74-75	0.5743061532784459
1102	76-77	0.5027669755611726
1102	78-79	0.27068767051126486
1102	80-81	0.33664578159828196
1102	82-83	0.4337373252034382
1102	84-85	0.5280229694480383
1102	86-87	0.4985517031492286
1102	88-89	0.5847995823079373
1102	90-91	0.3881564676436007
1102	92-93	0.33601146518976677
1102	94-95	0.6274578790537255
1102	96-97	0.3524441099670099
1102	98-99	0.4644919375286527
1102	100	0.34009966220125065
1103	1	-0.07990662895283407
1103	2	-0.003306982729689878
1103	3	0.04706272557198332
1103	4	0.13886748317129616
1103	5	0.1242017857975668
1103	6	0.17548054951888759
1103	7	0.2091412366144283
1103	8	0.11105148597308556
1103	9	0.055295522715844925
1103	10-11	-0.017638564568471793
1103	12-13	0.25170677855075496
1103	14-15	0.11927864678514055
1103	16-17	0.232461316314847
1103	18-19	0.2009423124615779
1103	20-21	0.05183428657935707
1103	22-23	0.3232478033106858
1103	24-25	0.34772204746028024
1103	26-27	0.46256392320012196
1103	28-29	0.40717463803363074
1103	30-31	0.06604238368846893
1103	32-33	0.16795022173458563
1103	34-35	0.1621789191205565
1103	36-37	0.011226745124687909
1103	38-39	0.257997531717578
1103	40-41	0.5190199365312722
1103	42-43	-0.07127877974731689
1103	44-45	0.17932180679986232
1103	46-47	0.6415134666332918
1103	48-49	0.26654204090053213
1103	50-51	-0.19033288022641415
1103	52-53	0.06991709958639802
1103	54-55	0.28330948563981906
1103	56-57	0.015570292201047664
1103	58-59	0.2309466437846126
1103	60-61	0.23280570745700402
1103	62-63	0.25355426706657624
1103	64-65	0.25445083314468775
1103	66-67	0.13098411727000325
1103	68-69	0.3775379904313638
1103	70-71	0.24885327980242877
1103	72-73	0.13138925823016123
1103	74-75	0.2686000274988807
1103	76-77	0.06390820071708703
1103	78-79	0.026938763359662943
1103	80-81	0.031114511562730485
1103	82-83	-0.05470762437982302
1103	84-85	0.020708170823567684
1103	86-87	0.2624818774221218
1103	88-89	0.2547156515508142
1103	90-91	0.20448507987184428
1103	92-93	0.1482309674337472
1103	94-95	0.24187182088504855
1103	96-97	0.35146783206294785
1103	98-99	0.3854571412355945
1103	100	0.314687294071625
1104	1	0.1373975804758487
1104	2	0.3097932861907893
1104	3	0.1668695418828321
1104	4	0.11768313544065734
1104	5	0.05966087146794763
1104	6	0.057128099049648995
1104	7	0.2247276478981064
1104	8	0.14708134163413433
1104	9	0.2824784435215122
1104	10-11	0.11214300614663841
1104	12-13	0.2211239345795164
1104	14-15	0.32953055392525954
1104	16-17	0.2554567611312706
1104	18-19	0.38726578537745127
1104	20-21	0.47218717610243033
1104	22-23	0.340782516004829
1104	24-25	0.4213458880837351
1104	26-27	0.3507972803155326
1104	28-29	0.49476587890953994
1104	30-31	0.5359809161510185
1104	32-33	0.9167428236774029
1104	34-35	1.0594266003674662
1104	36-37	1.0601538073056886
1104	38-39	1.0592433469856743
1104	40-41	0.9504589354885127
1104	42-43	0.960758430065539
1104	44-45	1.0509946019263623
1104	46-47	1.0304740066695075
1104	48-49	1.3667698000794957
1104	50-51	1.186581120102879
1104	52-53	1.539704707291797
1104	54-55	1.4792647021482423
1104	56-57	1.4527307114974661
1104	58-59	1.420768826979824
1104	60-61	1.4995583555004757
1104	62-63	1.6640970473750087
1104	64-65	1.7825914401366205
1104	66-67	1.8015428132801077
1104	68-69	1.908285479592223
1104	70-71	1.588914198522584
1104	72-73	1.5121724205154337
1104	74-75	1.4241760112538877
1104	76-77	1.2692893543310468
1104	78-79	1.3649416172096167
1104	80-81	1.3529135346679375
1104	82-83	1.257267569078273
1104	84-85	1.2966672290530852
1104	86-87	1.4106351072119239
1104	88-89	1.5205490306189233
1104	90-91	1.378254357079463
1104	92-93	1.5845544403496135
1104	94-95	1.5704075763320624
1104	96-97	1.8177018477591247
1104	98-99	1.688774741806366
1104	100	1.5900289328400774
1105	1	0.25644581800084865
1105	2	0.27108897156886513
1105	3	0.26877358635383075
1105	4	0.08768079759614977
1105	5	0.09872885079101223
1105	6	0.02448919212183398
1105	7	0.045466926283438625
1105	8	-0.13045976546511895
1105	9	0.07163084500734129
1105	10-11	-0.12221941170410133
1105	12-13	0.03631732627237483
1105	14-15	-0.1475650639150956
1105	16-17	-0.009304129327730948
1105	18-19	0.33341093954530265
1105	20-21	0.195139613954737
1105	22-23	0.28101811877907323
1105	24-25	0.16159837529052368
1105	26-27	0.28802615529280473
1105	28-29	0.35913193340729066
1105	30-31	0.20258480736332274
1105	32-33	0.06431925820476891
1105	34-35	-0.10686557824385545
1105	36-37	-0.2218292043575616
1105	38-39	-0.25351479001201227
1105	40-41	0.1336615227031004
1105	42-43	0.1721255495813523
1105	44-45	0.15533390109253276
1105	46-47	-0.5510453324997684
1105	48-49	-0.6282817623799133
1105	50-51	-0.6962863760656575
1105	52-53	-0.588967656788526
1105	54-55	-0.5946371603345497
1105	56-57	-0.614443354014135
1105	58-59	-0.8581684928113233
1105	60-61	-0.7665922536379028
1105	62-63	-0.6208537280100828
1105	64-65	-0.6605145161716628
1105	66-67	-0.4889018966972998
1105	68-69	-0.4592626410405529
1105	70-71	-0.21015456008198186
1105	72-73	-0.17754366125716814
1105	74-75	-0.15092074953266277
1105	76-77	-0.16268586451717226
1105	78-79	-0.0225755919235624
1105	80-81	-0.06871022822267747
1105	82-83	0.054375135901661764
1105	84-85	0.014788121590164849
1105	86-87	0.1681356916730543
1105	88-89	0.06702667822782615
1105	90-91	-0.19734244267116452
1105	92-93	-0.06271677558929056
1105	94-95	-0.08518246964554876
1105	96-97	-0.03255271753167932
1105	98-99	-0.21501646270307617
1105	100	-0.2753242115607826
1106	1	0.1497343749654121
1106	2	0.002067368641696987
1106	3	0.13988280393691355
1106	4	0.08583878466446748
1106	5	-0.11801467083697759
1106	6	0.021557008271400946
1106	7	0.13382089350312754
1106	8	0.11444023954715732
1106	9	0.08454999692955312
1106	10-11	0.12192248594514155
1106	12-13	-0.048396472534705026
1106	14-15	-0.0774808576096504
1106	16-17	0.05350600550260509
1106	18-19	-0.14107028946577316
1106	20-21	0.06247709077293706
1106	22-23	0.0523328398456826
1106	24-25	-0.10953465062296175
1106	26-27	-0.2686126096813837
1106	28-29	-0.22134648872692964
1106	30-31	-0.23380935834266126
1106	32-33	-0.21671302087898425
1106	34-35	-0.2681795474684563
1106	36-37	-0.18273968503521587
1106	38-39	-0.2852487883078325
1106	40-41	-0.6170464618590756
1106	42-43	-0.8463634985866619
1106	44-45	-1.2643004530751796
1106	46-47	-1.3262007131824447
1106	48-49	-1.1818191790507626
1106	50-51	-1.7185283689482205
1106	52-53	-1.681406632278474
1106	54-55	-1.6214215320452396
1106	56-57	-1.6507698539389537
1106	58-59	-1.469228088821552
1106	60-61	-1.431972435082443
1106	62-63	-1.387820296101907
1106	64-65	-1.407613659071771
1106	66-67	-1.0687537838207497
1106	68-69	-1.1212971255275477
1106	70-71	-1.4781799723420193
1106	72-73	-1.2396395461752192
1106	74-75	-1.1538028105822349
1106	76-77	-1.0153436260328874
1106	78-79	-1.152625464110418
1106	80-81	-1.301142186520508
1106	82-83	-1.096669924496311
1106	84-85	-0.9951236622803208
1106	86-87	-0.9167911290376871
1106	88-89	-0.8279911153664798
1106	90-91	-1.0437411194292245
1106	92-93	-1.3346267049161433
1106	94-95	-1.1527855973083199
1106	96-97	-0.8798410740050393
1106	98-99	-1.0894713772437008
1106	100	-1.0893686203623467
1107	1	0.05729896782055732
1107	2	-0.01637773909079243
1107	3	-0.03332865021848619
1107	4	-0.037027552430956234
1107	5	-0.1525496955548178
1107	6	-0.2266008128273782
1107	7	-0.24377914380682597
1107	8	-0.1309193351193798
1107	9	-0.038689439690166694
1107	10-11	-0.2501859927415495
1107	12-13	0.01508874339664601
1107	14-15	0.11510845308897899
1107	16-17	-0.04451423514659325
1107	18-19	-0.1542337070574149
1107	20-21	-0.10530110155986705
1107	22-23	-0.40151941274269376
1107	24-25	-0.14473425886845348
1107	26-27	-0.27852306579554664
1107	28-29	-0.1573948983902156
1107	30-31	-0.2379834092707398
1107	32-33	-0.1631382610618033
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2216	68-69	-0.4567268159696667
2216	70-71	-0.49677415777547296
2216	72-73	-0.49222860996975015
2216	74-75	-0.12785568397231017
2216	76-77	-0.5471749885394779
2216	78-79	-0.7885089468248871
2216	80-81	-0.6885791075116963
2216	82-83	-0.7980331842688528
2216	84-85	-0.674418683746012
2216	86-87	-0.9901416600311386
2216	88-89	-1.0860125057408112
2216	90-91	-1.2513369618635082
2216	92-93	-1.0062863418823262
2216	94-95	-1.1083855069264565
2216	96-97	-0.9564208736585726
2216	98-99	-0.803789370766335
2216	100	-0.804326818750539
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	3.0
4	11.0
5	41.0
6	97.0
7	191.0
8	245.0
9	275.0
10	397.0
11	707.0
12	1088.0
13	1685.0
14	1744.0
15	2211.0
16	3109.0
17	4548.0
18	5281.0
19	2718.0
20	1271.0
21	1001.0
22	953.0
23	1088.0
24	1305.0
25	1593.0
26	1896.0
27	2293.0
28	1763.0
29	2059.0
30	2341.0
31	2943.0
32	3515.0
33	4576.0
34	6301.0
35	12550.0
36	18587.0
37	43022.0
38	53158.0
39	8891.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.180035331165676	16.88676105379042	14.257917509408843	16.675286105635063
2	15.944097440698169	25.138247315623353	35.12990899464404	23.78774624903444
3	26.353293842014264	26.723652844704993	31.538831425268306	15.384221888012442
4	11.649546765975732	17.342595813468108	39.386855190194595	31.621002230361565
5	9.597502622757862	41.213889102121236	33.5595302064942	15.629078068626697
6	24.85906919165567	37.92650113051574	14.978054694453824	22.23637498337477
7	21.109280839787598	37.469178117230584	22.27258310398101	19.14895793900081
8	24.907154476535403	25.409492239853492	21.01685031153126	28.666502972079844
9	23.058940282168543	18.876543588799198	24.8406535506379	33.22386257839436
10-11	24.93995718352512	23.80764813758565	25.787334610495407	25.465060068393818
12-13	31.947300573954145	23.337732625354246	26.67786951495248	18.037097285739133
14-15	25.05703733259671	23.97690883234605	33.74512752831405	17.220926306743195
16-17	17.515832335819447	27.378942737587348	33.33716992521204	21.768055001381175
18-19	16.715562023781814	26.819892012451053	37.44702292497398	19.017523038793158
20-21	21.101511105654623	20.24595111670401	36.591622929519254	22.060914848122117
22-23	18.265246616125964	21.523024666728052	38.463879766325974	21.747848950820007
24-25	22.478905085031883	21.629225697046582	37.095334436569466	18.79653478135207
26-27	20.567667942133372	18.37541691391623	43.09329663808803	17.96361850586237
28-29	17.55950325493356	24.630318842803973	39.636777551829546	18.173400350432924
30-31	19.47398317401183	20.943442572615805	38.35869854461343	21.22387570875893
32-33	19.083692145530645	21.000171411174474	40.71859657740494	19.197539865889944
34-35	21.30815775671285	19.68274679868493	39.728799682746796	19.28029576185542
36-37	20.16818977250632	19.206739871260886	42.064328622450546	18.560741733782248
38-39	19.94079903395485	21.05753290079617	40.21700198530465	18.78466607994433
40-41	18.975870476833766	22.279959779863834	38.80398824098309	19.94018150231931
42-43	18.659435120753173	22.63277732296357	36.06170691772411	22.646080638559148
44-45	20.224776592124886	19.950777354513058	36.81541763777352	23.009028415588535
46-47	22.708145207294645	22.643425586505575	35.656418114331174	18.99201109186861
48-49	21.625133654274123	21.79012314099055	35.064230790875186	21.52051241386014
50-51	17.633170906393485	23.09429715177327	38.202560462469144	21.069971479364106
52-53	20.422351478690587	24.80828946627992	32.52633302127616	22.24302603375333
54-55	18.129731941886636	21.875895232248823	34.18866380192347	25.80570902394107
56-57	21.51926254456909	21.11334489444095	32.667034985139374	24.700357575850585
58-59	23.971660229685142	20.7742282016523	32.58741079878252	22.66670076988004
60-61	20.157864178489852	22.80180781596495	35.78339970688213	21.256928298663066
62-63	18.386300563989103	23.913649559423476	34.453211924341055	23.246837952246366
64-65	17.4623109151273	27.07731186151084	32.485510100725904	22.97486712263596
66-67	19.285367163721002	23.10714377062179	30.79392280737652	26.813566258280687
68-69	18.501769965828405	23.87868060813161	32.386538028687774	25.23301139735221
70-71	22.249021664066294	23.68033352942681	30.459881832365653	23.610762974141238
72-73	20.482645727293654	20.994961250223803	35.141826738624445	23.380566283858094
74-75	18.716014016420697	26.588996598204467	31.175281990945596	23.51970739442924
76-77	20.02148502442643	21.891705245926797	31.473514591912423	26.613295137734355
78-79	20.78128357017894	22.868901097776824	32.9458375535844	23.40397777845984
80-81	22.241973767674082	22.28545558715803	31.62611773853615	23.846452906631747
82-83	20.26355098115447	21.342411655173827	34.635315422234044	23.758721941437663
84-85	19.95882036984935	23.36419674143796	32.42576156738369	24.251221321329
86-87	20.104252989983937	24.289718956856245	31.268479584215747	24.337548468944068
88-89	19.78735050080313	23.512681215023072	29.5399670564644	27.1600012277094
90-91	20.84011131231904	21.004061671935588	31.042120663372312	27.11370635237306
92-93	22.409021617916373	23.497590620300173	30.935974954728216	23.157412807055238
94-95	20.81734753383874	22.43971435294599	31.363627062807563	25.3793110504077
96-97	17.30712173761804	22.929775022252233	34.05461260652937	25.708490633600363
98-99	20.27971312523659	24.31580778163142	29.3560664190786	26.048412674053385
100	19.54257593894192	20.30324422209263	30.343349395864667	29.810830443100784
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	539.0
1	353.5
2	561.5
3	1169.0
4	826.0
5	351.5
6	709.5
7	1372.5
8	2075.0
9	2232.0
10	1396.0
11	546.5
12	360.5
13	322.0
14	302.0
15	296.5
16	316.0
17	339.0
18	353.5
19	370.5
20	384.0
21	446.0
22	492.5
23	575.0
24	725.0
25	862.5
26	1033.5
27	1240.0
28	1476.0
29	1689.5
30	1902.0
31	2185.5
32	2386.0
33	2523.0
34	2647.0
35	2625.0
36	2640.5
37	2768.5
38	2760.5
39	2997.0
40	3474.5
41	3892.5
42	4238.0
43	4265.5
44	4116.0
45	4057.5
46	4840.5
47	11093.5
48	24261.0
49	24135.5
50	13774.5
51	8841.0
52	5348.5
53	4040.0
54	3691.5
55	2992.5
56	2282.5
57	1939.5
58	1746.0
59	1634.5
60	1541.5
61	1597.0
62	1693.5
63	1724.0
64	1423.5
65	917.0
66	577.0
67	379.5
68	279.5
69	234.0
70	159.5
71	142.5
72	172.0
73	169.5
74	154.5
75	175.0
76	165.0
77	94.5
78	63.0
79	80.5
80	75.0
81	50.5
82	49.5
83	22.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09770520651095219
2	0.0015346367514809244
3	0.0
4	0.0010230911676539496
5	0.04143519228998496
6	0.0
7	0.004092364670615798
8	0.0
9	0.0
10-11	2.557727919134874E-4
12-13	0.0
14-15	0.0
16-17	0.0
18-19	2.557727919134874E-4
20-21	0.0
22-23	0.0
24-25	2.557727919134874E-4
26-27	0.0010230911676539496
28-29	0.00690586538166416
30-31	0.03785437320319614
32-33	0.025321506399435256
34-35	0.029925416653878024
36-37	0.02660037035900269
38-39	0.02557727919134874
40-41	0.03146005340535895
42-43	0.02353109685604084
44-45	0.024298415231781303
46-47	0.014067503555241808
48-49	0.009719366092712522
50-51	0.00690586538166416
52-53	0.004603910254442773
54-55	0.003069273502961849
56-57	0.0015346367514809244
58-59	5.115455838269748E-4
60-61	7.673183757404622E-4
62-63	0.001790409543394412
64-65	0.002557727919134874
66-67	5.115455838269748E-4
68-69	0.0010230911676539496
70-71	5.115455838269748E-4
72-73	5.115455838269748E-4
74-75	5.115455838269748E-4
76-77	5.115455838269748E-4
78-79	0.0
80-81	0.0010230911676539496
82-83	0.0010230911676539496
84-85	5.115455838269748E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	195486.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.197927217294332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.19463744716361	21.76728768300543
2	9.050668504979772	5.104201835425555
3	3.615550677575604	3.0585310457014825
4	2.151551983745442	2.4267722496751687
5	1.4331585726466267	2.0206050561165507
6	0.9977686265261324	1.688100426629017
7	0.7002521633437948	1.382196167500486
8	0.6331295466502186	1.4282352700449137
9	0.4771148159570415	1.2108283969184495
>10	3.3162200896177643	17.172073703487715
>50	0.2539774685702883	4.850475225847376
>100	0.11247573608112765	6.6541849544212885
>500	0.021769497306024708	4.411057569340004
>1k	0.0399107450610453	22.884503238083546
>5k	0.0018141247755020591	3.940947177803014
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	7704	3.940947177803014	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	4647	2.377152328043952	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4181	2.1387720859805817	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3729	1.9075534820907891	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	3117	1.5944875847886806	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2443	1.2497058612892995	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	2306	1.1796241163050039	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	1980	1.01286025597741	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	1958	1.0016062531332166	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	1936	0.9903522502890233	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	1913	0.9785867018610028	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1807	0.9243628699753434	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	1765	0.9028779554546106	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	1678	0.8583734896616637	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	1585	0.810799750365755	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	1373	0.7023520865944364	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1341	0.6859826279119732	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	1259	0.6440358900381613	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT	1256	0.6425012532866804	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	1199	0.6133431550085428	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1121	0.5734425994700387	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	1081	0.5529807761169598	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	1061	0.5427498644404203	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	984	0.5033608544857432	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	893	0.4568102063574885	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	858	0.4389061109235444	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	806	0.41230574056454167	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	774	0.39593628188207847	No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC	716	0.366266638020114	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	691	0.3534779984244396	No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	612	0.3130658973021086	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	602	0.3079504414638389	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	598	0.3059042591285309	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	580	0.2966964386196454	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	509	0.2603767021679302	No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT	482	0.24656497140460187	No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	460	0.23531096856040842	TruSeq Adapter, Index 1 (95% over 21bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	417	0.2133145084558485	No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT	409	0.20922214378523268	No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG	383	0.19592195860573136	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	358	0.183133319010057	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	353	0.1805755910909221	No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT	331	0.16932158824672866	No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA	317	0.162159950073151	No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG	316	0.16164840448932405	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	314	0.1606253133216701	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	308	0.15755603981870825	No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT	305	0.1560214030672273	No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC	304	0.15550985748340035	No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT	299	0.15295212956426546	RNA PCR Primer, Index 1 (95% over 23bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	281	0.14374430905537994	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	266	0.1360711252979753	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	264	0.13504803413032135	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	254	0.1299325782920516	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	234	0.1197016666155121	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	227	0.11612084752872329	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	221	0.11305157402576145	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	214	0.10947075493897261	No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA	214	0.10947075493897261	No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG	207	0.1058899358521838	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	201	0.10282066234922195	No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA	198	0.10128602559774103	No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA	197	0.10077448001391404	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	197	0.10077448001391404	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.115455838269748E-4	0.0
3	0.0	0.0	0.0	5.115455838269748E-4	0.0
4	0.0	0.0	0.0	0.0010230911676539496	0.0
5	0.0	0.0	0.0	0.0010230911676539496	0.0
6	0.0	0.0	0.0	0.0010230911676539496	0.0
7	0.0	0.0	0.0	0.0010230911676539496	0.0
8	0.0	0.0	0.0	0.0010230911676539496	0.0
9	0.0	0.0	0.0	0.0010230911676539496	0.0
10-11	0.0	0.0	0.0	0.0010230911676539496	0.0
12-13	0.0	0.0	0.0	0.0015346367514809244	0.0
14-15	0.0	0.0	0.0	0.0015346367514809244	0.0
16-17	0.0	0.0	0.0	0.0015346367514809244	0.0
18-19	0.0	0.0	0.0	0.0015346367514809244	0.0
20-21	0.0	0.0	0.0	0.003069273502961849	0.0
22-23	0.0	0.0	0.0	0.009207820508885547	0.0
24-25	0.0	0.0	0.0	0.021229141728819456	0.0
26-27	0.0	0.0	0.0	0.04782951208782214	0.0
28-29	0.0	0.0	0.0	0.10870343656323214	0.0
30-31	0.0	0.0	0.0	0.24349569790164	0.0
32-33	0.0	0.0	0.0	0.4488812498081704	0.0
34-35	0.0	0.0	0.0	0.7466007795954697	0.0
36-37	0.0	0.0	0.0	1.1683701134608104	0.0
38-39	0.0	0.0	0.0	1.707539158814442	0.0
40-41	0.0	0.0	0.0	2.31832458590385	0.0
42-43	0.0	0.0	0.0	2.810687210337313	0.0
44-45	0.0	0.0	0.0	3.2708224629896767	0.0
46-47	0.0	0.0	0.0	3.785948865903441	0.0
48-49	0.0	0.0	0.0	4.314886999580533	0.0
50-51	0.0	0.0	0.0	4.817992081274363	0.0
52-53	0.0	0.0	0.0	5.27991774347012	0.0
54-55	0.0	0.0	0.0	5.734937540284214	0.0
56-57	0.0	0.0	0.0	6.305055093459378	0.0
58-59	0.0	0.0	0.0	6.805602447234072	0.0
60-61	0.0	0.0	0.0	7.323798123650798	0.0
62-63	0.0	0.0	0.0	7.793652742395874	0.0
64-65	0.0	0.0	0.0	8.24432440174744	0.0
66-67	0.0	0.0	0.0	8.777354900095148	0.0
68-69	0.0	0.0	0.0	9.415763788711212	0.0
70-71	0.0	0.0	0.0	10.074378727888442	0.0
72-73	0.0	0.0	0.0	10.727366665643576	0.0
74-75	0.0	0.0	0.0	11.384191195277412	0.0
76-77	0.0	0.0	0.0	11.969910888759298	0.0
78-79	0.0	0.0	0.0	12.813961102073804	0.0
80-81	0.0	0.0	0.0	13.636014855283754	0.0
82-83	0.0	0.0	0.0	14.360619174774664	0.0
84-85	0.0	0.0	0.0	15.149422465035858	0.0
86-87	0.0	0.0	0.0	15.896279017423243	0.0
88	0.0	0.0	0.0	16.50859908126413	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	25	4.8868424E-7	94.06119	2
TACGAGT	25	4.8868424E-7	94.06119	4
GGGTACG	30	1.4350226E-6	78.48479	1
ACGAGTG	30	1.4460002E-6	78.38432	5
GTACGAG	30	1.4460002E-6	78.38432	3
CGAGTGT	30	1.4504094E-6	78.34421	6
TCCTAAC	30	1.3639285E-4	62.70746	2
TAACAGT	30	1.3639285E-4	62.70746	4
ACAGTGT	30	1.3673886E-4	62.675365	6
ACGTGCG	30	1.3691212E-4	62.659332	9
GTGTGCG	40	8.014669E-6	58.743126	9
TTATGCC	25	0.0052161026	56.436714	4
ATAACGC	25	0.0052161026	56.436714	3
ATTTGAT	25	0.0052161026	56.436714	2
TATAACG	25	0.0052161026	56.436714	2
TGATGTT	25	0.0052161026	56.436714	5
CTAACAC	35	2.9220598E-4	53.749252	4
TAACACG	35	2.9220598E-4	53.749252	5
GTCCTAA	45	1.5906828E-5	52.323193	1
CTGTAAC	40	5.611382E-4	47.090874	1
>>END_MODULE